FastQCFastQC Report
Wed 25 May 2016
SRR1780305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452553
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCAAA300.00573653829.33827211
TATACCG450.001401798224.4296516
CGAAACT551.5944285E-423.99342725
GACGAAA1600.022.00370423
TATGCAG500.002582067621.99397730
GCTATCA500.00258538421.9891171
GTAGATC1800.020.78817438
GACCCAA1201.4300895E-820.16114428
AGATCTC2350.019.6694140
ACCCAAT901.0020563E-519.55020129
TAGATCT2050.019.32673639
TCAGATG809.014552E-519.238354
TAGAGGA708.145684E-418.8457325
ATACTGG708.145684E-418.8457326
GATGGAA600.007374358418.34656321
GATCACA600.00743101718.32223942
CGTCAGA851.4337523E-418.1086832
TTAGGCC851.4349664E-418.1066825
CAGTAGC750.001293461817.5912931
TAAGGAT750.001294420317.589354
Produced by FastQC (version 0.11.5)