FastQCFastQC Report
Wed 25 May 2016
SRR1780305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452553
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT279266.170768948609334No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG75451.6672080397213143No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC69311.531533323168778No Hit
CACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCTG45831.01269906508188RNA PCR Primer, Index 13 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC14380.317752837789165No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT6550.14473442889562108TruSeq Adapter, Index 8 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG7200.037.5845644
GTCTTCT7200.037.5845643
CGTCTTC7450.036.32333442
GCTTATA505.9246304E-835.2050363
AACGATT250.00235214135.19725822
CCGTCTT8100.033.408541
ATGCCGT37100.032.7935244
TCTTTAT1900.032.4831281
TAACGGC300.00574344929.3310536
GCGCTAT300.00574344929.3310519
GCCGTCT9450.028.63585540
TATGCCG45950.026.4774743
TGCCGTC10400.026.4431739
CTTTATA2250.026.403782
TTAATAC508.322003E-526.4037783
TTTATAC2350.026.2165183
ATATCGT801.3070348E-724.74807437
CTTATAT450.001381380424.4912471
CAGCTTA752.0255357E-623.5115971
ATCGTAT852.3451685E-723.29487839
Produced by FastQC (version 0.11.5)