FastQCFastQC Report
Wed 25 May 2016
SRR1780304_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780304_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289028
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGTA200.00694679952.526
TGTCTAG200.00694679952.512
AAGCAAG400.00241644835.025
ACGCTTC400.00241644835.016
CTAAAGC450.004303178731.1111093
GTTAGAC500.00720158227.9999983
TACGGAT500.00720158227.9999987
GTAAGGT500.00720158227.99999819
TAGATCT906.154627E-627.22222139
AGGAATT700.001224333425.00000215
CATTAAA750.001829727823.33333461
GGTCTAG750.001829727823.3333341
GTGTAGT800.002662365621.8751
CGAATGT7450.021.6107470
GCGTAGT850.003784003420.5882368
ACGAATG11100.020.1801870
TGTATGG900.005267501319.4444432
AGATCTC1451.112441E-519.31034540
CTATGAT950.00719787418.4210514
GTAGAAC950.00719787418.4210513
Produced by FastQC (version 0.11.5)