##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780304_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 289028 Sequences flagged as poor quality 0 Sequence length 76 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.236987419903954 34.0 33.0 34.0 21.0 34.0 2 31.25441133730988 34.0 33.0 34.0 23.0 34.0 3 31.12392224974743 34.0 33.0 34.0 23.0 34.0 4 30.88565813692791 34.0 32.0 34.0 21.0 34.0 5 30.970729479496796 34.0 32.0 34.0 21.0 34.0 6 34.0663084545442 38.0 34.0 38.0 23.0 38.0 7 34.37939576788408 38.0 35.0 38.0 24.0 38.0 8 34.41426782180273 38.0 35.0 38.0 25.0 38.0 9 34.757594419917794 38.0 36.0 38.0 26.0 38.0 10-11 34.51170128845648 38.0 35.5 38.0 24.5 38.0 12-13 34.574617338112574 38.0 35.5 38.0 25.0 38.0 14-15 34.57420388336078 38.0 35.5 38.0 25.0 38.0 16-17 34.64793895401138 38.0 36.0 38.0 25.0 38.0 18-19 34.65663534328854 38.0 36.0 38.0 25.0 38.0 20-21 34.68471739762238 38.0 36.0 38.0 25.0 38.0 22-23 34.72091112279779 38.0 36.0 38.0 25.0 38.0 24-25 34.71270430546521 38.0 36.0 38.0 25.5 38.0 26-27 34.716825013493505 38.0 36.0 38.0 25.0 38.0 28-29 34.82804434172468 38.0 36.0 38.0 26.0 38.0 30-31 34.82549095589355 38.0 36.0 38.0 26.0 38.0 32-33 34.90305783522704 38.0 36.0 38.0 26.0 38.0 34-35 34.826807437341714 38.0 36.0 38.0 25.5 38.0 36-37 34.749136761836226 38.0 36.0 38.0 25.0 38.0 38-39 34.75809956128818 38.0 36.0 38.0 25.5 38.0 40-41 34.82506539158835 38.0 36.0 38.0 25.5 38.0 42-43 34.863023651687726 38.0 36.0 38.0 26.0 38.0 44-45 34.95486423460703 38.0 36.0 38.0 27.0 38.0 46-47 34.91800621393083 38.0 36.0 38.0 26.5 38.0 48-49 34.89603602419143 38.0 36.0 38.0 26.0 38.0 50-51 34.82664655327511 38.0 36.0 38.0 26.0 38.0 52-53 34.85419059745077 38.0 36.0 38.0 26.0 38.0 54-55 34.851908811603025 38.0 36.0 38.0 26.0 38.0 56-57 34.786605449991 38.0 36.0 38.0 25.5 38.0 58-59 34.86559087700846 38.0 36.0 38.0 26.0 38.0 60-61 34.8714000027679 38.0 36.0 38.0 26.0 38.0 62-63 34.72994311969775 38.0 36.0 38.0 25.0 38.0 64-65 34.71986624133302 38.0 36.0 38.0 25.0 38.0 66-67 34.579807146712426 38.0 36.0 38.0 25.0 38.0 68-69 34.52627427100489 38.0 36.0 38.0 25.0 38.0 70-71 34.52880689760162 38.0 35.5 38.0 25.0 38.0 72-73 34.576267697247324 38.0 36.0 38.0 25.0 38.0 74-75 34.601943410327024 38.0 36.0 38.0 25.0 38.0 76 32.60160261289564 37.0 31.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09848846082270768 1101 2 0.3946291425947983 1101 3 0.48659462100936324 1101 4 0.49674126692988096 1101 5 -0.35051161750624615 1101 6 0.08807968357352536 1101 7 -0.3533326634172198 1101 8 -0.5108618763057677 1101 9 -0.3375105358031192 1101 10-11 -0.41220290655761005 1101 12-13 0.23011607742239448 1101 14-15 -0.21624828869249058 1101 16-17 -0.3779375937452656 1101 18-19 -0.2916938106311946 1101 20-21 -0.4542438992045774 1101 22-23 -0.35038565527913335 1101 24-25 -0.3329466467199964 1101 26-27 -0.5617535065895822 1101 28-29 -0.1931237965750583 1101 30-31 -0.30413178249940387 1101 32-33 -0.1093798269148536 1101 34-35 -0.27612576714218307 1101 36-37 -0.16771991674340114 1101 38-39 0.17010030655725927 1101 40-41 0.08135008968834256 1101 42-43 -0.3324382464815372 1101 44-45 0.024081211637977162 1101 46-47 0.268388281608388 1101 48-49 0.027993618842693024 1101 50-51 -0.12599511784836182 1101 52-53 -0.1489706850912853 1101 54-55 -0.19903977092251068 1101 56-57 -0.01469919661608543 1101 58-59 -0.16137965388711706 1101 60-61 -0.24786317089440502 1101 62-63 -0.48571008794471027 1101 64-65 -0.10878869237169653 1101 66-67 -0.47421651057469916 1101 68-69 -0.24465333527069788 1101 70-71 -0.24707354266702453 1101 72-73 -0.13942605757471682 1101 74-75 -0.4440496809687957 1101 76 -1.4631677327668804 1102 1 0.2661756477085504 1102 2 0.08622376697140766 1102 3 0.48413109115082875 1102 4 0.28990700017082105 1102 5 0.10910710337322271 1102 6 0.1405311229857844 1102 7 -0.24260689583616823 1102 8 0.08591119169771844 1102 9 0.16822877470063702 1102 10-11 0.13163429274325011 1102 12-13 0.2830511346856639 1102 14-15 0.059578608574419434 1102 16-17 0.10207178649358895 1102 18-19 0.07788922314578173 1102 20-21 0.1765406490427992 1102 22-23 0.011271238952993201 1102 24-25 0.2764004854099156 1102 26-27 -0.024432976687236874 1102 28-29 0.1449940536299934 1102 30-31 0.3810078418690992 1102 32-33 0.3674982088826084 1102 34-35 0.23695382369763962 1102 36-37 0.20775254381658925 1102 38-39 0.34293932185938303 1102 40-41 0.31384206861039843 1102 42-43 0.06122100417045573 1102 44-45 0.1856785462220074 1102 46-47 -0.3684244495503961 1102 48-49 -0.008560912315765279 1102 50-51 0.20906766321394343 1102 52-53 0.32559393712689655 1102 54-55 0.0175277108079257 1102 56-57 0.34576407033610224 1102 58-59 0.06553367952194833 1102 60-61 0.2484188140160697 1102 62-63 0.024563545631998807 1102 64-65 0.3442289694171379 1102 66-67 0.3709332730144297 1102 68-69 0.35436741972493024 1102 70-71 0.33298468622053434 1102 72-73 0.23443407300210595 1102 74-75 -0.07804184895621091 1102 76 -0.08728955253321402 1103 1 0.360817959927914 1103 2 0.29258629367265954 1103 3 0.6628768952399327 1103 4 0.6363148645066659 1103 5 0.29947067632758007 1103 6 -0.43273928984825005 1103 7 0.1489533291082452 1103 8 0.5961339564215535 1103 9 0.415395172538382 1103 10-11 0.085157768047317 1103 12-13 0.4482773869198553 1103 14-15 0.17613774367185187 1103 16-17 0.25447053970863465 1103 18-19 0.24240162560447231 1103 20-21 0.38256532317854663 1103 22-23 -0.0819815183598891 1103 24-25 0.24554146801595778 1103 26-27 -0.04136374960671674 1103 28-29 0.36089819068914863 1103 30-31 0.1601231040862885 1103 32-33 0.4005696156570906 1103 34-35 0.14084021804787739 1103 36-37 0.23406145004615553 1103 38-39 -0.3685781810832083 1103 40-41 0.11087986450385046 1103 42-43 0.054485749800171845 1103 44-45 0.27763983485707655 1103 46-47 0.26102757538530597 1103 48-49 0.10694652044067254 1103 50-51 -0.24441221821070513 1103 52-53 0.15758312584480194 1103 54-55 0.1876146805973704 1103 56-57 0.4400085884057603 1103 58-59 -0.005938485786046499 1103 60-61 0.45984912919981724 1103 62-63 0.11308636702787567 1103 64-65 0.21670597364573752 1103 66-67 0.19870475389851094 1103 68-69 0.3341889027887035 1103 70-71 0.4406070760370042 1103 72-73 0.4567737565105432 1103 74-75 0.2815150192594942 1103 76 0.3331734144662164 1104 1 0.14980615677715647 1104 2 0.1406573076706117 1104 3 -0.07802077827125942 1104 4 0.6339554536887384 1104 5 -0.17773997577211276 1104 6 0.2501241935444227 1104 7 0.037750090557793214 1104 8 -0.5584714671749964 1104 9 0.04369347034845816 1104 10-11 0.1450971671596406 1104 12-13 -0.02233057289090823 1104 14-15 -0.0589985778420683 1104 16-17 -0.10901894963438963 1104 18-19 0.282859119629741 1104 20-21 -0.2960274437336139 1104 22-23 -0.1070845520622683 1104 24-25 0.04935676333859362 1104 26-27 0.12601419508430922 1104 28-29 -0.050117880129754155 1104 30-31 -0.04403299502116198 1104 32-33 -0.16155543921292548 1104 34-35 0.15596300495710835 1104 36-37 0.01802294348613742 1104 38-39 0.16208196621231252 1104 40-41 0.01164438676887869 1104 42-43 0.02798353061273673 1104 44-45 0.12096459220965983 1104 46-47 0.21198902005193787 1104 48-49 -0.07607242800589376 1104 50-51 0.03407141833028504 1104 52-53 -0.06300349562522456 1104 54-55 -0.14681808533931928 1104 56-57 0.08721570443756121 1104 58-59 0.17924173174309743 1104 60-61 0.05821278329921853 1104 62-63 0.20283847647527153 1104 64-65 0.2076585171759575 1104 66-67 0.4080948431537408 1104 68-69 0.2193459529764823 1104 70-71 0.2536954045742945 1104 72-73 0.18983111397042052 1104 74-75 0.10420255279117185 1104 76 0.16247888195877636 1105 1 0.38367935374152395 1105 2 0.28300288740625135 1105 3 0.6668465384096471 1105 4 0.5228143268857011 1105 5 0.590692451226932 1105 6 0.5590401105168326 1105 7 0.576474521376511 1105 8 0.004094497543306375 1105 9 0.3367024666795686 1105 10-11 0.759125035682537 1105 12-13 -0.3626456957737503 1105 14-15 0.27456301435776 1105 16-17 0.2669108387759209 1105 18-19 0.15755128427767318 1105 20-21 0.21909104146515546 1105 22-23 0.4488566702325514 1105 24-25 0.059496482894303426 1105 26-27 0.1381391385431172 1105 28-29 0.0016267296104004458 1105 30-31 0.43836070754706213 1105 32-33 0.20744491259890196 1105 34-35 0.46484436898183645 1105 36-37 0.10290068887488957 1105 38-39 0.502792877404957 1105 40-41 0.3258626106217264 1105 42-43 0.5367411322612199 1105 44-45 0.5421556009044366 1105 46-47 0.4161812349587066 1105 48-49 0.5697944112161011 1105 50-51 0.3832383781734734 1105 52-53 0.49019233791752015 1105 54-55 0.47970852671274145 1105 56-57 0.5277752108958254 1105 58-59 0.27547483221050584 1105 60-61 0.42922348843064384 1105 62-63 0.7959775175367341 1105 64-65 0.164850556176809 1105 66-67 0.5637087547987178 1105 68-69 0.4214241557206151 1105 70-71 0.5355971988146351 1105 72-73 0.49974592524850436 1105 74-75 0.42985151997215354 1105 76 0.29316441244075975 1106 1 -0.09482261751466581 1106 2 -0.11639376964555836 1106 3 0.6343806417714895 1106 4 0.6153972393065068 1106 5 0.5477593731702264 1106 6 0.6443457496950771 1106 7 -0.24407188816613257 1106 8 0.09752133433840982 1106 9 0.5146476997664422 1106 10-11 3.5200095567944345E-4 1106 12-13 0.4512217451200513 1106 14-15 0.30084590536555567 1106 16-17 0.4079454018876092 1106 18-19 0.4881019764216248 1106 20-21 0.6589866215686939 1106 22-23 0.5862258304648194 1106 24-25 0.5066756341834946 1106 26-27 0.24847752702544312 1106 28-29 0.5108343224105951 1106 30-31 0.152756860763418 1106 32-33 0.5425726348848059 1106 34-35 0.5398213814895527 1106 36-37 0.6241499488665809 1106 38-39 0.3934070528152631 1106 40-41 0.5423024463713659 1106 42-43 0.3074377477466044 1106 44-45 0.49458723673217975 1106 46-47 0.5368529735688767 1106 48-49 0.42168725801233364 1106 50-51 0.4724266992678139 1106 52-53 0.5873585784233768 1106 54-55 0.4796959308265656 1106 56-57 0.5168262368307524 1106 58-59 0.5132158859823406 1106 60-61 0.6927483212202574 1106 62-63 0.4353935092738368 1106 64-65 0.6435477636688418 1106 66-67 0.2896238459299525 1106 68-69 0.5259511172149942 1106 70-71 0.7073106172121797 1106 72-73 0.9958128597876907 1106 74-75 0.4385836180689111 1106 76 0.8109875913905142 1107 1 -0.17767659149767212 1107 2 -0.27912217027718356 1107 3 -0.3449849746909024 1107 4 -0.4855689987565057 1107 5 -0.29135133690822457 1107 6 -0.9039912179967331 1107 7 -0.23268945339660263 1107 8 -0.7000856285911325 1107 9 0.03954305530695734 1107 10-11 -0.21325741829890177 1107 12-13 0.028762036370018507 1107 14-15 -0.20448187617190428 1107 16-17 -0.09085463320274556 1107 18-19 -0.18632529881210758 1107 20-21 -0.08841293228247338 1107 22-23 0.031760582451184405 1107 24-25 0.006796257287987828 1107 26-27 -0.2128196883040374 1107 28-29 -0.06019388773051304 1107 30-31 -0.4065317448961494 1107 32-33 -0.11203798746775817 1107 34-35 -0.09009910125350729 1107 36-37 -0.0390390127094804 1107 38-39 -0.4994279347777848 1107 40-41 0.0901584881790285 1107 42-43 -0.07060132787312057 1107 44-45 -0.23620862511207008 1107 46-47 -0.08697212606266902 1107 48-49 0.07677410664756934 1107 50-51 0.06502451363981976 1107 52-53 0.1434796526896065 1107 54-55 -0.12439084261504973 1107 56-57 -0.28067733486636826 1107 58-59 0.0617737349434222 1107 60-61 -0.14445748372748568 1107 62-63 0.03745318794641861 1107 64-65 0.10805480680492252 1107 66-67 -0.016653881718745822 1107 68-69 0.09622739112030132 1107 70-71 -0.03050801576773665 1107 72-73 0.0578491657756075 1107 74-75 -0.08507887535164826 1107 76 0.15690332440302512 1108 1 -0.3394438782649587 1108 2 -0.1184663846213958 1108 3 -0.21440309410902003 1108 4 0.2107279475404411 1108 5 0.24589370033681135 1108 6 0.3434445294390187 1108 7 0.16670507099792076 1108 8 -0.0773671308726378 1108 9 -0.38663962087571946 1108 10-11 0.24895641891493625 1108 12-13 0.1299720125799979 1108 14-15 0.5015382673482449 1108 16-17 0.25029333294521905 1108 18-19 0.30132631383951036 1108 20-21 -0.030273624143163147 1108 22-23 0.08503463572523628 1108 24-25 0.0574451204368529 1108 26-27 0.2765089260245759 1108 28-29 -0.05561869565532618 1108 30-31 0.30216477630036565 1108 32-33 0.044586169156403344 1108 34-35 0.23999479134037927 1108 36-37 -0.04974452341499358 1108 38-39 0.28974516689531526 1108 40-41 0.2439624544829968 1108 42-43 0.2737641414923573 1108 44-45 -0.10790580930925842 1108 46-47 0.1145313254407796 1108 48-49 0.126967156840621 1108 50-51 0.14362421723951968 1108 52-53 0.07177564348559429 1108 54-55 0.27118655296234806 1108 56-57 0.34767179348276045 1108 58-59 0.2736292592989429 1108 60-61 0.10969602042602133 1108 62-63 0.21192473741796647 1108 64-65 0.4448713311214405 1108 66-67 0.35814868058381677 1108 68-69 0.5181468755397844 1108 70-71 0.31484707958735925 1108 72-73 0.02719269761913523 1108 74-75 0.14914804637528079 1108 76 0.14487181237151248 1109 1 0.6907295153137731 1109 2 0.32817539238886795 1109 3 0.1799883930397712 1109 4 0.25776972520483454 1109 5 0.01082545002078561 1109 6 0.4569819187070294 1109 7 0.5040024337082443 1109 8 0.5548285064055918 1109 9 0.4994566769105404 1109 10-11 0.5942987661632557 1109 12-13 0.009973451666567712 1109 14-15 0.4478170020640775 1109 16-17 0.29481752677055795 1109 18-19 0.3504833852329199 1109 20-21 0.3752356244410535 1109 22-23 0.44340562243167625 1109 24-25 -0.3149512367117282 1109 26-27 0.2821859590205449 1109 28-29 0.03322200563772526 1109 30-31 0.20492831311727144 1109 32-33 0.21142536267920775 1109 34-35 0.43403599173482377 1109 36-37 -0.14823773436055632 1109 38-39 0.4466489394280586 1109 40-41 -0.11737828360489289 1109 42-43 0.5295985745821881 1109 44-45 0.2079638814016036 1109 46-47 0.1472795392563384 1109 48-49 0.12696470385214553 1109 50-51 0.2571346236343004 1109 52-53 0.16223695249836823 1109 54-55 0.44967253576852784 1109 56-57 0.04025755404818909 1109 58-59 0.30875033063347956 1109 60-61 0.19140751954539326 1109 62-63 -0.07052462063494858 1109 64-65 0.40610920722590294 1109 66-67 0.72274535204199 1109 68-69 0.24482181695424288 1109 70-71 0.5681115984173104 1109 72-73 0.18033113284143099 1109 74-75 0.5055582776266832 1109 76 0.17496834564466468 1110 1 -0.040787902628785844 1110 2 0.2074889908389288 1110 3 0.5061376375703261 1110 4 0.011430701213491545 1110 5 0.04874517274274126 1110 6 0.34665884700977045 1110 7 -0.31744792924418874 1110 8 0.04425130510223596 1110 9 0.308544955496977 1110 10-11 -0.16802164472154146 1110 12-13 0.42259516968334765 1110 14-15 0.10808063007872448 1110 16-17 -0.07560310805022397 1110 18-19 -0.04139830611283912 1110 20-21 0.09444285329608704 1110 22-23 0.20232138853179293 1110 24-25 0.3550498326455198 1110 26-27 0.08269736340113809 1110 28-29 0.03218410006826389 1110 30-31 -0.16098844056572403 1110 32-33 0.12433564989598267 1110 34-35 -0.058145905933976394 1110 36-37 0.1807704682386131 1110 38-39 0.10081958997469087 1110 40-41 -0.031672444914285336 1110 42-43 -0.09516003374370285 1110 44-45 0.13581607735817158 1110 46-47 -0.2706842472747937 1110 48-49 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-1.0287391292337915 2116 5 -0.9768387496046138 2116 6 -0.025791266164240767 2116 7 0.205275398488304 2116 8 0.35903821857618823 2116 9 -0.11411484203958366 2116 10-11 0.33223785703508923 2116 12-13 0.4744443149765658 2116 14-15 0.5197476207609597 2116 16-17 0.36114963103855047 2116 18-19 0.32068909477311536 2116 20-21 0.21619343999571328 2116 22-23 0.3089575167308638 2116 24-25 0.2337227805854809 2116 26-27 -0.08014476232616374 2116 28-29 -0.09316826686548296 2116 30-31 0.2338065964414895 2116 32-33 -0.021943494147450338 2116 34-35 0.1696627883295534 2116 36-37 0.05038255216790333 2116 38-39 0.19929226157564983 2116 40-41 -0.30428294579607496 2116 42-43 -0.04893834460424529 2116 44-45 0.03981865328196932 2116 46-47 0.24518181163123387 2116 48-49 0.25068715012583453 2116 50-51 0.4634990911115864 2116 52-53 0.32530226166859677 2116 54-55 0.1573040870462208 2116 56-57 0.02044043469459922 2116 58-59 0.1072929169457737 2116 60-61 -0.16136215455922098 2116 62-63 0.07186993607285075 2116 64-65 -0.41117766518001275 2116 66-67 0.1648800763755247 2116 68-69 -0.05256328563321233 2116 70-71 -0.11634889298309048 2116 72-73 -0.057698845897931506 2116 74-75 0.1943555460007289 2116 76 0.3176765546012206 2117 1 0.08413836659388352 2117 2 -0.5968365480979436 2117 3 -0.18430369379685274 2117 4 -0.3494954977893876 2117 5 -0.4568144789671109 2117 6 0.47600018539892375 2117 7 0.10987985672589673 2117 8 -0.08598182459371628 2117 9 -0.03029360921055968 2117 10-11 0.2298814168612111 2117 12-13 -0.23448210719327278 2117 14-15 -0.06214091335221639 2117 16-17 -0.22189374775249604 2117 18-19 0.07743464101166353 2117 20-21 -0.45109292368587006 2117 22-23 0.06152632498288568 2117 24-25 -0.148729747440143 2117 26-27 0.041376529190060296 2117 28-29 -0.48867819892759456 2117 30-31 0.21135725145028772 2117 32-33 -0.4946728466983572 2117 34-35 -0.059175503574593336 2117 36-37 -0.18520277802218033 2117 38-39 0.21644085954845593 2117 40-41 0.011765515903078949 2117 42-43 0.21821771360549747 2117 44-45 -0.16527320710218874 2117 46-47 0.1035178803847785 2117 48-49 -0.34521170895526865 2117 50-51 0.22051022178509783 2117 52-53 -0.061401606712934154 2117 54-55 -0.2514701462900959 2117 56-57 -0.545474240088808 2117 58-59 -0.04567576000607687 2117 60-61 -0.2698926868222671 2117 62-63 0.01401132248327741 2117 64-65 -0.3879773230038026 2117 66-67 -0.14915710544536864 2117 68-69 -0.48686147627707754 2117 70-71 -0.30926365728933547 2117 72-73 -2.3449259255633166 2117 74-75 -0.6598773924373944 2117 76 -1.0766019410171381 2118 1 0.2243796855586062 2118 2 0.02084175468674232 2118 3 0.1344522547463285 2118 4 -0.5595228227103313 2118 5 -0.07129361685050384 2118 6 0.14290632890081412 2118 7 0.15169005598290397 2118 8 0.40182634832083863 2118 9 -0.059663293899390624 2118 10-11 -0.9237552537967346 2118 12-13 -0.22574734313936062 2118 14-15 8.752666852416269E-4 2118 16-17 0.18313526578715056 2118 18-19 0.1951682076814052 2118 20-21 0.26115416728463003 2118 22-23 0.18976924045983878 2118 24-25 0.18491674790848123 2118 26-27 -0.017473367957713037 2118 28-29 0.36197422193759365 2118 30-31 0.07678355091913858 2118 32-33 0.13399375856398876 2118 34-35 -0.18362363227804224 2118 36-37 0.24595377228330761 2118 38-39 -0.029099651949508143 2118 40-41 0.2199384304589671 2118 42-43 0.07063243836065425 2118 44-45 0.23285703145359093 2118 46-47 -0.21143100052155006 2118 48-49 -0.13462560475213792 2118 50-51 0.1240071976225039 2118 52-53 0.15096522267518253 2118 54-55 -0.05151926974347987 2118 56-57 0.0930600388710019 2118 58-59 0.19299667866636128 2118 60-61 0.013190423920420358 2118 62-63 -0.15212112662789679 2118 64-65 0.09515798140809295 2118 66-67 0.09743341986855825 2118 68-69 0.17658453397744012 2118 70-71 0.2981355628758422 2118 72-73 0.08770125812769436 2118 74-75 -0.08210268487545846 2118 76 0.062386729886426906 2119 1 0.43749227271699453 2119 2 0.3001803973841639 2119 3 -0.36815946771272223 2119 4 0.12054943751460101 2119 5 0.7857170569540628 2119 6 0.5505927900223924 2119 7 0.7891067529415849 2119 8 0.37032265269500897 2119 9 0.7285283278800136 2119 10-11 0.8468647962126212 2119 12-13 0.4998257267085222 2119 14-15 0.45420904062969925 2119 16-17 0.5661794480679774 2119 18-19 0.11388096227202027 2119 20-21 0.02796072798432192 2119 22-23 -0.15506184024910397 2119 24-25 0.27275257620232196 2119 26-27 0.50993264418112 2119 28-29 0.23843457244374378 2119 30-31 0.12980779554644073 2119 32-33 0.2267390963475009 2119 34-35 0.2606555829935431 2119 36-37 0.20609168356625673 2119 38-39 0.09875908812297496 2119 40-41 0.4709501837607988 2119 42-43 0.30373555237979133 2119 44-45 0.12813808627662837 2119 46-47 -0.18719188086257788 2119 48-49 0.02868250359413338 2119 50-51 0.07269472722606451 2119 52-53 0.15749287653108013 2119 54-55 0.07375859064888601 2119 56-57 0.10340779120349453 2119 58-59 -0.16959433506968935 2119 60-61 -0.0981586171996085 2119 62-63 0.2133129304092165 2119 64-65 0.01719294390107251 2119 66-67 -0.23855129092530802 2119 68-69 -0.5238329450659975 2119 70-71 -0.1669757465102819 2119 72-73 0.04636312213307292 2119 74-75 0.08451110845589227 2119 76 0.06156058402211073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 15.0 13 281.0 14 1098.0 15 1854.0 16 2423.0 17 2989.0 18 2933.0 19 2718.0 20 2274.0 21 1843.0 22 1588.0 23 1549.0 24 1678.0 25 1995.0 26 2430.0 27 2841.0 28 3639.0 29 4548.0 30 5596.0 31 6976.0 32 8959.0 33 11799.0 34 15980.0 35 23851.0 36 44148.0 37 133022.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.00498221625586 17.625627966840582 9.33888758182598 28.03050223507757 2 24.04230731970605 17.948434061751804 27.835711418962866 30.173547199579282 3 23.157617947050113 17.974383104751094 24.206305271461588 34.6616936767372 4 18.531422561135948 15.340728233942732 26.789446005231326 39.33840319969 5 20.81390038335386 28.42458170142685 27.85958453852222 22.901933376697066 6 36.37225459125067 26.97904701274617 14.709993495439889 21.938704900563266 7 34.38351993578477 22.59677263102537 17.39969829912673 25.62000913406314 8 29.20478292760563 27.37381845357543 16.722601270465145 26.698797348353793 9 26.577009839877103 14.534577964764658 16.933999474099394 41.95441272125884 10-11 31.599879596440484 19.256092835296236 20.743145992775787 28.400881575487496 12-13 29.47447998117829 17.280678688569964 21.055572470487288 32.189268859764454 14-15 27.736240087465575 18.156372392986146 19.092613864400683 35.01477365514759 16-17 29.678785446392737 20.16621227009148 19.622493322446267 30.532508961069517 18-19 29.05081860580982 19.398466584552363 20.217764368850077 31.332950440787744 20-21 28.653279267060633 20.0184757186155 20.53192078276153 30.796324231562338 22-23 30.050029754902642 19.584088738807313 19.863819422339706 30.502062083950342 24-25 29.50250494761753 19.870220186279532 19.428221487191554 31.199053378911383 26-27 28.494817111144943 20.384876205765533 20.635543961138715 30.484762721950815 28-29 29.182120763386248 20.035256099755042 20.994332729008956 29.78829040784976 30-31 29.050991599429814 19.45330556209087 20.786913378634598 30.70878945984472 32-33 27.87895982396169 20.49161326930263 21.4192050597174 30.210221847018282 34-35 28.459353419045907 20.043213806274824 21.52352021257456 29.973912562104708 36-37 28.37752743678813 19.297957291335095 21.551199191773808 30.773316080102965 38-39 27.328148137896672 19.607269883886683 23.18079909212948 29.88378288608716 40-41 27.223486997799522 19.108356283820253 23.503086206180715 30.16507051219951 42-43 26.328936988803854 18.735901019970385 23.805825041172483 31.129336950053283 44-45 25.132513112916392 19.147452841939188 25.396328383409223 30.323705661735197 46-47 25.103450184757186 19.624915233126202 25.56430518842465 29.70732939369196 48-49 24.551254549732207 20.039926927494914 25.23855820197351 30.17026032079937 50-51 23.69303320093555 21.23358290546245 24.9669582185809 30.106425675021104 52-53 23.296877811146324 21.48788352685553 24.94550700970148 30.26973165229666 54-55 23.341510165105113 21.007826231368586 24.831850201364574 30.818813402161727 56-57 23.15727195981012 21.445154102716693 24.827352367244696 30.57022157022849 58-59 23.82935217349184 21.21732150518289 24.2168578822813 30.736468439043968 60-61 23.800635232572624 20.52136817194182 24.313907303098663 31.3640892923869 62-63 23.719674218414823 20.818225223853744 24.21426297798137 31.24783757975006 64-65 24.256992402120208 20.971843558409567 23.230794248308122 31.540369791162103 66-67 24.336569467318046 21.42612480451721 22.238848831255105 31.998456896909644 68-69 24.224642595181088 22.222068450115557 22.411842451250397 31.14144650345295 70-71 24.589140152511174 22.78429771510027 21.936109996263337 30.690452136125216 72-73 24.59709785903096 23.01870407019389 21.86103076518538 30.523167305589773 74-75 24.22395062070111 23.860145037851005 22.60040549704527 29.315498844402622 76 24.854339371963963 23.387353474403863 22.360117358871808 29.39818979476037 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 3.0 15 5.0 16 13.0 17 21.0 18 21.5 19 28.0 20 35.5 21 37.0 22 56.0 23 118.5 24 167.5 25 173.0 26 274.0 27 455.0 28 602.0 29 669.0 30 844.5 31 1161.0 32 1635.5 33 1969.0 34 2226.5 35 2795.5 36 3412.5 37 3718.0 38 4171.5 39 5307.5 40 6494.0 41 7343.5 42 7689.0 43 7941.0 44 8637.0 45 9283.5 46 9486.0 47 9509.0 48 9333.5 49 8793.0 50 8451.0 51 8179.5 52 7355.0 53 6600.5 54 6399.0 55 6508.5 56 6800.5 57 7508.0 58 8033.0 59 8506.0 60 9663.0 61 10786.0 62 11225.0 63 11455.0 64 12390.5 65 13368.0 66 13909.0 67 14178.0 68 14145.0 69 13461.5 70 12226.5 71 11642.0 72 10786.5 73 8822.5 74 6651.5 75 5589.0 76 4483.0 77 2559.5 78 1250.0 79 758.0 80 502.0 81 170.0 82 65.0 83 36.0 84 35.0 85 24.5 86 14.5 87 14.0 88 12.0 89 10.5 90 10.0 91 10.5 92 12.0 93 11.0 94 8.5 95 8.0 96 9.0 97 7.5 98 5.5 99 26.0 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 289028.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.95036625162001 #Duplication Level Percentage of deduplicated Percentage of total 1 74.91990953983122 50.159153832299396 2 16.00943576015635 21.436751752485 3 4.907802150048988 9.857374543088035 4 1.6804016625898304 4.500140270408813 5 0.7142488826593771 2.390961214442783 6 0.4408426461820965 1.7708745972734818 7 0.29966155954557117 1.4043715814175337 8 0.21709007468425257 1.16274080077618 9 0.15599597877958615 0.9399589121765911 >10 0.6540964844954309 6.358354223515153 >50 5.152610273269663E-4 0.019318272117046766 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0017299361999529458 0.0 2 0.0 0.0 0.0 0.003805859639896481 0.0 3 0.0 0.0 0.0 0.006227770319830604 0.0 4 0.0 0.0 0.0 0.03252280055911538 0.0 5 0.0 0.0 0.0 0.035636685719030683 0.0 6 0.0 0.0 0.0 0.03598267295902127 0.0 7 0.0 0.0 0.0 0.03702063467899304 0.0 8 0.0 0.0 0.0 0.03736662191898363 0.0 9 0.0 0.0 0.0 0.043594392238814235 0.0 10 0.0 0.0 0.0 0.047054264638720124 0.0 11 0.0 0.0 0.0 0.0477462391187013 0.0 12 0.0 0.0 0.0 0.04809222635869189 0.0 13 0.0 0.0 0.0 0.04843821359868248 0.0 14 0.0 0.0 0.0 0.05051413703862601 0.0 15 0.0 0.0 0.0 0.05120611151860719 0.0 16 0.0 0.0 0.0 0.052244073238578964 0.0 17 0.0 0.0 0.0 0.05328203495855073 0.0 18 0.0 0.0 0.0 0.05570394563848485 0.0 19 0.0 0.0 0.0 0.06020177975836251 0.0 20 0.0 0.0 0.0 0.0636616521582684 0.0 21 0.0 0.0 0.0 0.06815948627814607 6.919744799811783E-4 22 0.0 0.0 0.0 0.07819311623787314 6.919744799811783E-4 23 0.0 0.0 0.0 0.08303693759774139 6.919744799811783E-4 24 0.0 0.0 0.0 0.09410852927744025 6.919744799811783E-4 25 0.0 0.0 0.0 0.10587209543712028 6.919744799811783E-4 26 0.0 0.0 0.0 0.12317145743664974 6.919744799811783E-4 27 0.0 0.0 0.0 0.21831794843406174 6.919744799811783E-4 28 0.0 0.0 0.0 0.37089832126991157 6.919744799811783E-4 29 0.0 0.0 0.0 0.5362802219854131 6.919744799811783E-4 30 0.0 0.0 0.0 0.6888605948212629 6.919744799811783E-4 31 0.0 0.0 0.0 0.8839973981759552 6.919744799811783E-4 32 0.0 0.0 0.0 1.0808641377306005 6.919744799811783E-4 33 0.0 0.0 0.0 1.3375866698036176 6.919744799811783E-4 34 0.0 0.0 0.0 1.6814979863542632 6.919744799811783E-4 35 0.0 0.0 0.0 2.1478887858615776 6.919744799811783E-4 36 0.0 0.0 0.0 2.8748079770818054 6.919744799811783E-4 37 0.0 0.0 0.0 3.874019126174627 6.919744799811783E-4 38 0.0 0.0 0.0 4.849011168468107 6.919744799811783E-4 39 0.0 0.0 0.0 5.898736454599554 0.0017299361999529458 40 0.0 0.0 0.0 6.980292566810136 0.0017299361999529458 41 0.0 0.0 0.0 8.12170447153909 0.0017299361999529458 42 0.0 0.0 0.0 9.317782360186555 0.0017299361999529458 43 0.0 0.0 0.0 10.567142283792574 0.0017299361999529458 44 0.0 0.0 0.0 11.753878516960295 0.0017299361999529458 45 0.0 0.0 0.0 12.881796919329615 0.0017299361999529458 46 0.0 0.0 0.0 13.973386661499925 0.0017299361999529458 47 0.0 0.0 0.0 15.056326722670468 0.0017299361999529458 48 0.0 0.0 0.0 16.04861812696348 0.0017299361999529458 49 0.0 0.0 0.0 17.017036411697138 0.0017299361999529458 50 0.0 3.4598723999058916E-4 0.0 17.92213903151252 0.0017299361999529458 51 0.0 3.4598723999058916E-4 0.0 18.86841413288678 0.0017299361999529458 52 0.0 3.4598723999058916E-4 0.0 19.852055856180023 0.0017299361999529458 53 0.0 3.4598723999058916E-4 0.0 20.832237707073364 0.0017299361999529458 54 0.0 3.4598723999058916E-4 0.0 21.78058873188757 0.0017299361999529458 55 0.0 3.4598723999058916E-4 0.0 22.700914790262534 0.0017299361999529458 56 0.0 3.4598723999058916E-4 0.0 23.5551572857993 0.0017299361999529458 57 0.0 3.4598723999058916E-4 0.0 24.428429079535547 0.0017299361999529458 58 0.0 3.4598723999058916E-4 0.0 25.28751539643218 0.0017299361999529458 59 0.0 3.4598723999058916E-4 0.0 26.079826176010627 0.0017299361999529458 60 0.0 3.4598723999058916E-4 0.0 26.856221542549513 0.0017299361999529458 61 0.0 3.4598723999058916E-4 0.0 27.679325186487123 0.0017299361999529458 62 0.0 3.4598723999058916E-4 0.0 28.43565329310655 0.0017299361999529458 63 0.0 3.4598723999058916E-4 0.0 29.253221141204314 0.0017299361999529458 64 0.0 3.4598723999058916E-4 0.0 30.035152303583043 0.0017299361999529458 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTGTA 20 0.006946799 52.5 26 TGTCTAG 20 0.006946799 52.5 12 AAGCAAG 40 0.002416448 35.0 25 ACGCTTC 40 0.002416448 35.0 16 CTAAAGC 45 0.0043031787 31.111109 3 GTTAGAC 50 0.007201582 27.999998 3 TACGGAT 50 0.007201582 27.999998 7 GTAAGGT 50 0.007201582 27.999998 19 TAGATCT 90 6.154627E-6 27.222221 39 AGGAATT 70 0.0012243334 25.000002 15 CATTAAA 75 0.0018297278 23.333334 61 GGTCTAG 75 0.0018297278 23.333334 1 GTGTAGT 80 0.0026623656 21.875 1 CGAATGT 745 0.0 21.61074 70 GCGTAGT 85 0.0037840034 20.588236 8 ACGAATG 1110 0.0 20.18018 70 TGTATGG 90 0.0052675013 19.444443 2 AGATCTC 145 1.112441E-5 19.310345 40 CTATGAT 95 0.007197874 18.421051 4 GTAGAAC 95 0.007197874 18.421051 3 >>END_MODULE