FastQCFastQC Report
Wed 25 May 2016
SRR1780304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289028
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT81662.8253318017631512No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC23290.8058042819380822No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG22610.7822771496187221No Hit
CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG18540.6414603429425523TruSeq Adapter, Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC5720.19790470127461698No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTTC150.002223214870.041
GAGTAAC150.002223214870.011
TAACGTC207.918594E-570.014
GTAACGT207.918594E-570.013
AGTAACG150.002223214870.012
TATGCTG200.00694679952.55
GTAACTG200.00694679952.543
GTAGTGA200.00694679952.543
ATATTAA352.083562E-550.00000433
TCTTTAT1000.048.9999961
CTTTATA1100.047.7272762
ATCGTAT305.8720604E-446.66666441
GGACTAT459.1848124E-538.8888851
CTTAATA501.7073234E-435.02
GAATCTC400.00241644835.019
TTATCAA400.00241644835.032
TTATATA601.2134036E-535.02
GGTGAAT400.00241644835.08
TGTACTT400.00241644835.063
CTTGAAA16950.033.65781857
Produced by FastQC (version 0.11.5)