##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780304_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 289028 Sequences flagged as poor quality 0 Sequence length 76 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.708772160482724 34.0 34.0 34.0 34.0 34.0 2 33.70758542424955 34.0 34.0 34.0 34.0 34.0 3 33.700589562256944 34.0 34.0 34.0 34.0 34.0 4 33.73719155237555 34.0 34.0 34.0 34.0 34.0 5 33.72162212657597 34.0 34.0 34.0 34.0 34.0 6 37.440331040591225 38.0 38.0 38.0 37.0 38.0 7 37.464982631440556 38.0 38.0 38.0 37.0 38.0 8 37.349353695835696 38.0 38.0 38.0 36.0 38.0 9 37.36298905296373 38.0 38.0 38.0 37.0 38.0 10-11 37.31460100751484 38.0 38.0 38.0 36.0 38.0 12-13 37.299218414824864 38.0 38.0 38.0 36.0 38.0 14-15 37.223701509888315 38.0 38.0 38.0 36.0 38.0 16-17 37.26807956322571 38.0 38.0 38.0 36.0 38.0 18-19 37.23270928768147 38.0 38.0 38.0 36.0 38.0 20-21 37.25506179332106 38.0 38.0 38.0 36.0 38.0 22-23 37.21606384156553 38.0 38.0 38.0 36.0 38.0 24-25 37.23717252307735 38.0 38.0 38.0 36.0 38.0 26-27 37.1434203606571 38.0 38.0 38.0 36.0 38.0 28-29 37.105996650843515 38.0 38.0 38.0 36.0 38.0 30-31 37.06839821747374 38.0 38.0 38.0 36.0 38.0 32-33 37.072259435072034 38.0 38.0 38.0 36.0 38.0 34-35 37.07006587597049 38.0 38.0 38.0 36.0 38.0 36-37 37.06174834272112 38.0 38.0 38.0 36.0 38.0 38-39 36.78613663728082 38.0 38.0 38.0 35.0 38.0 40-41 36.91439929695393 38.0 38.0 38.0 35.0 38.0 42-43 36.985892370289385 38.0 38.0 38.0 35.5 38.0 44-45 37.035472341780036 38.0 38.0 38.0 36.0 38.0 46-47 36.98595810786498 38.0 38.0 38.0 35.5 38.0 48-49 37.01961401663507 38.0 38.0 38.0 36.0 38.0 50-51 36.88113954357363 38.0 38.0 38.0 35.0 38.0 52-53 36.899680999764726 38.0 38.0 38.0 35.0 38.0 54-55 36.87789418326252 38.0 37.5 38.0 35.0 38.0 56-57 36.91597007902349 38.0 38.0 38.0 35.0 38.0 58-59 36.8308260791342 38.0 37.5 38.0 35.0 38.0 60-61 36.858197129689856 38.0 38.0 38.0 35.0 38.0 62-63 36.802965456633956 38.0 37.0 38.0 35.0 38.0 64-65 36.79281765088504 38.0 37.5 38.0 34.5 38.0 66-67 36.588745727057585 38.0 37.0 38.0 34.0 38.0 68-69 36.55717093153605 38.0 37.0 38.0 34.0 38.0 70-71 36.191695960252986 38.0 37.0 38.0 33.0 38.0 72-73 35.712624728400016 38.0 37.0 38.0 31.0 38.0 74-75 35.55059890391242 38.0 37.0 38.0 30.5 38.0 76 34.587780422658014 38.0 35.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.016857890372904194 1101 2 0.037534833539162094 1101 3 -0.01388243732496619 1101 4 0.0348577279808282 1101 5 -0.08358369752168215 1101 6 -0.10826740401660118 1101 7 0.0659585168650807 1101 8 -0.17350302842943677 1101 9 -0.16540680188435886 1101 10-11 -0.026280984361235937 1101 12-13 0.09526146654570056 1101 14-15 0.1663831130770319 1101 16-17 0.09670709707038583 1101 18-19 -0.012160774286570586 1101 20-21 -0.10580505931500994 1101 22-23 -0.09941389175734372 1101 24-25 -0.06754973331859304 1101 26-27 -0.02038579196743484 1101 28-29 -0.23896544678939335 1101 30-31 -0.12072252810284567 1101 32-33 -0.09348797604325654 1101 34-35 -0.03847299166146456 1101 36-37 -0.1818152754468514 1101 38-39 0.02207379433285439 1101 40-41 -0.176684319761506 1101 42-43 7.528926278794756E-4 1101 44-45 -0.011570595531452454 1101 46-47 -0.08517049699975132 1101 48-49 0.05624846264371541 1101 50-51 0.049519812998582324 1101 52-53 -0.22721725666252013 1101 54-55 -0.08140492882138517 1101 56-57 -0.1657178160013899 1101 58-59 -0.09158863643138204 1101 60-61 -0.20584766120786213 1101 62-63 -0.027160108138005512 1101 64-65 -0.19951537072518022 1101 66-67 -0.20375161585322132 1101 68-69 -0.5159524356376863 1101 70-71 -0.41623220728777 1101 72-73 -0.08371935636753136 1101 74-75 0.12460807802554541 1101 76 -1.325597085473575 1102 1 0.07249943343521181 1102 2 0.11615519190323909 1102 3 0.0391435011452117 1102 4 0.07097976196715905 1102 5 0.0306312592529423 1102 6 0.07149886899378544 1102 7 0.06196136664006957 1102 8 -0.015186343126714519 1102 9 0.02268646170846722 1102 10-11 -0.09574100393315632 1102 12-13 -2.9200811872698296E-4 1102 14-15 0.11626375034904157 1102 16-17 0.07190220766145927 1102 18-19 0.1293794025098336 1102 20-21 0.11088981839296252 1102 22-23 0.049626418926550286 1102 24-25 0.020038652712322857 1102 26-27 0.092779162291734 1102 28-29 -0.017956957510946836 1102 30-31 0.10544833830340394 1102 32-33 0.15391176656818573 1102 34-35 0.17384822796433497 1102 36-37 0.03392210383125871 1102 38-39 0.17855906102862207 1102 40-41 0.27350060726575975 1102 42-43 0.23737672070659954 1102 44-45 0.17927831608496092 1102 46-47 0.20526230851933747 1102 48-49 0.27071756222193244 1102 50-51 0.18600877985777942 1102 52-53 0.23282990419235006 1102 54-55 0.07212426433201102 1102 56-57 0.03397578450105243 1102 58-59 0.2963872930318132 1102 60-61 0.30779344566031597 1102 62-63 0.007756126978165412 1102 64-65 0.057480453607936965 1102 66-67 0.04494318395657615 1102 68-69 0.17265933769809294 1102 70-71 0.15575544585112056 1102 72-73 0.28575169877950657 1102 74-75 0.3342807596216133 1102 76 0.6226038723494298 1103 1 -0.08197547828717688 1103 2 0.08651135389809639 1103 3 0.052428735898253365 1103 4 -0.001996392180799944 1103 5 0.11585729351772756 1103 6 -0.08297604272861037 1103 7 0.03523831855602566 1103 8 0.02862550568971045 1103 9 0.08570141440910817 1103 10-11 0.0611969619764281 1103 12-13 0.09328770328978919 1103 14-15 0.15373268970995468 1103 16-17 0.103687594993076 1103 18-19 0.1959799517927152 1103 20-21 0.09446037508680405 1103 22-23 0.13205154643864603 1103 24-25 0.03931804588551557 1103 26-27 0.12174729516588201 1103 28-29 0.06645169151473596 1103 30-31 0.22592547331365154 1103 32-33 0.20873651917291625 1103 34-35 0.06602421645554557 1103 36-37 0.16489991454663766 1103 38-39 0.031026474603763177 1103 40-41 0.2420530724996368 1103 42-43 0.1866715897069966 1103 44-45 0.1594812455936534 1103 46-47 0.1794710796078789 1103 48-49 0.10815598586724917 1103 50-51 0.06571146960013863 1103 52-53 0.1954645002950528 1103 54-55 0.13868666897040072 1103 56-57 -0.019617441010090886 1103 58-59 0.27703161056963665 1103 60-61 0.17136640001752568 1103 62-63 0.13072356235024074 1103 64-65 0.27589123873370625 1103 66-67 -0.06677703052530859 1103 68-69 0.07352140666360896 1103 70-71 0.06848594848854361 1103 72-73 -0.10626626189881705 1103 74-75 0.08838948275688097 1103 76 0.0818217981526459 1104 1 0.011480357444177969 1104 2 -0.23510621426266454 1104 3 0.07712024445797283 1104 4 0.0400767190712088 1104 5 0.051933721357556806 1104 6 0.10560001931109753 1104 7 0.10693514341092225 1104 8 -0.17880145621830223 1104 9 -0.02971462226845034 1104 10-11 0.033347378988572984 1104 12-13 0.061133872143123824 1104 14-15 0.026873205731995142 1104 16-17 0.03425873092439957 1104 18-19 0.032217331688336515 1104 20-21 0.031134276467085442 1104 22-23 0.042689623793179976 1104 24-25 0.022610125093436295 1104 26-27 0.14100972017684654 1104 28-29 0.1105306067954217 1104 30-31 0.06266582838363632 1104 32-33 0.1505407673317194 1104 34-35 0.20765288055348918 1104 36-37 0.0805403086353067 1104 38-39 -0.07939943867722121 1104 40-41 0.022551658072480052 1104 42-43 -0.003690796285304998 1104 44-45 0.08794293412704235 1104 46-47 -4.2027775445774296E-4 1104 48-49 0.11000327650764774 1104 50-51 -0.13571941856256586 1104 52-53 0.018350175562090953 1104 54-55 -0.10689934283274738 1104 56-57 -0.34622818090181795 1104 58-59 0.10666629817632156 1104 60-61 -0.027793479024566636 1104 62-63 0.03203373647110652 1104 64-65 -0.3198508477902138 1104 66-67 -0.1911887019880254 1104 68-69 -0.09236895084504937 1104 70-71 -0.09678864547331045 1104 72-73 0.12577904854355637 1104 74-75 0.08167095376113309 1104 76 0.38333255267637156 1105 1 0.06786448570471748 1105 2 0.05960530003597597 1105 3 0.0596678647022344 1105 4 0.08552955115644778 1105 5 0.13247978357799184 1105 6 0.1203447044395034 1105 7 0.06517005319631863 1105 8 -6.219904385318387E-5 1105 9 0.09263422429133072 1105 10-11 0.10051830927957184 1105 12-13 0.06805702638748556 1105 14-15 0.03315555985573582 1105 16-17 0.13847042112895025 1105 18-19 0.17831347683584653 1105 20-21 0.2435657988071398 1105 22-23 0.10130712894470406 1105 24-25 -0.13580885766779005 1105 26-27 0.13175267137552282 1105 28-29 0.15933821598274278 1105 30-31 0.061607251713788 1105 32-33 0.08229869566262948 1105 34-35 0.1378716321240745 1105 36-37 0.08878903959977436 1105 38-39 -0.053801986800053214 1105 40-41 0.07626264465336874 1105 42-43 0.0510410728406967 1105 44-45 -0.13280014271872176 1105 46-47 0.0841496322822266 1105 48-49 0.004038809502567631 1105 50-51 -0.04981764945164713 1105 52-53 0.07915708112574293 1105 54-55 -0.16918260161548204 1105 56-57 0.10740176331087525 1105 58-59 0.11024095767272968 1105 60-61 0.2937939462154233 1105 62-63 0.2183526538958347 1105 64-65 0.10564043230225906 1105 66-67 -0.11138081637712816 1105 68-69 0.2718254000621201 1105 70-71 0.11448692370797175 1105 72-73 0.3581444805182272 1105 74-75 0.24692827898603298 1105 76 0.25913669400421213 1106 1 0.10880426477287841 1106 2 0.022863100038492234 1106 3 0.09017825001038915 1106 4 0.050723968659696084 1106 5 0.09107395672104701 1106 6 0.15034180738568637 1106 7 0.12443999563311792 1106 8 0.28184947860222564 1106 9 0.2316266793555073 1106 10-11 0.22527426398476535 1106 12-13 0.29028467183848505 1106 14-15 0.11061396194161688 1106 16-17 0.20244965051264074 1106 18-19 0.2723071285092118 1106 20-21 0.2830444550780058 1106 22-23 0.1735445362074941 1106 24-25 0.2409309491113234 1106 26-27 0.1497821079615349 1106 28-29 0.27293264104351067 1106 30-31 0.2428352876372486 1106 32-33 0.17209476826531045 1106 34-35 0.14748071879637337 1106 36-37 0.23717802575689007 1106 38-39 0.23315276877677604 1106 40-41 0.3195521862890729 1106 42-43 0.2493648752868225 1106 44-45 0.2085452238845491 1106 46-47 0.1769545471970133 1106 48-49 0.328055385688792 1106 50-51 0.23707097772272334 1106 52-53 0.3165407266271245 1106 54-55 0.3577930631324122 1106 56-57 0.22343191786239913 1106 58-59 0.2522391060774609 1106 60-61 0.30860985733884405 1106 62-63 0.3715894817478613 1106 64-65 0.4113882500649808 1106 66-67 0.3810679498558187 1106 68-69 0.5067607201865698 1106 70-71 0.40091422655086717 1106 72-73 0.5733105572775585 1106 74-75 0.5665000818352297 1106 76 0.7368230816300141 1107 1 0.07656186559498934 1107 2 0.13280557691645356 1107 3 0.0710571381473386 1107 4 0.11792200359966643 1107 5 0.10291381936735888 1107 6 0.055720031312297635 1107 7 0.11308575847242963 1107 8 0.06523544747206245 1107 9 0.09039738893267213 1107 10-11 0.062444038654533074 1107 12-13 0.05553331208712109 1107 14-15 0.16697471588301482 1107 16-17 0.2034443994912607 1107 18-19 -0.08760715075990078 1107 20-21 0.17484239727917128 1107 22-23 0.1358114359743965 1107 24-25 0.18452006608753635 1107 26-27 0.11078794799963276 1107 28-29 0.15619767350209202 1107 30-31 0.03644445624642145 1107 32-33 0.1738743506900562 1107 34-35 -0.005718456580225961 1107 36-37 0.07359586419086384 1107 38-39 0.06875287655429929 1107 40-41 0.12111151405807874 1107 42-43 0.08368669146347685 1107 44-45 0.0950686466982944 1107 46-47 -0.007727258452533192 1107 48-49 0.07970024620461658 1107 50-51 0.1562660299822909 1107 52-53 0.15753284382891763 1107 54-55 0.06082367946948608 1107 56-57 -0.16356616470019958 1107 58-59 0.11822370391689674 1107 60-61 -0.2959390435810221 1107 62-63 -0.1877194888514282 1107 64-65 0.34874726202076545 1107 66-67 0.30112302918509215 1107 68-69 0.2487776638164192 1107 70-71 0.02908019141037954 1107 72-73 0.11212143278198283 1107 74-75 -0.034265639898237055 1107 76 -0.09481526210510083 1108 1 -0.1553567413236152 1108 2 -0.07125725214637413 1108 3 -0.03532034323013988 1108 4 0.021435907113563246 1108 5 0.045471636925178416 1108 6 -0.10230325171098542 1108 7 0.023522168908840513 1108 8 0.13318326541988057 1108 9 0.07831225184753521 1108 10-11 0.005345544056041263 1108 12-13 0.04025992181373539 1108 14-15 0.014226188134486506 1108 16-17 0.0872949708418318 1108 18-19 0.22197515882240282 1108 20-21 0.20084323578000607 1108 22-23 0.06298617564913656 1108 24-25 0.17220092876839743 1108 26-27 0.18456627177795326 1108 28-29 0.19886859117301015 1108 30-31 0.1442064390083999 1108 32-33 0.10520503202073428 1108 34-35 0.1475102591484898 1108 36-37 0.24962010271509882 1108 38-39 0.1876079329093585 1108 40-41 0.18301188845845218 1108 42-43 0.25039479567157485 1108 44-45 0.15639133302097719 1108 46-47 0.29487404414877005 1108 48-49 0.1960714875758569 1108 50-51 0.10465435337060569 1108 52-53 0.05830455710063376 1108 54-55 -0.11088617974037618 1108 56-57 0.10777904414501904 1108 58-59 0.014505537698724424 1108 60-61 0.0905294053874286 1108 62-63 0.15824985711790873 1108 64-65 0.13847611424961315 1108 66-67 0.1749018404639031 1108 68-69 0.07742124245999804 1108 70-71 0.19574929557948195 1108 72-73 0.10069685885741109 1108 74-75 -0.06283316846576525 1108 76 0.8384897961999513 1109 1 0.0459812821236838 1109 2 0.06437829263783357 1109 3 0.09873384164996679 1109 4 0.002729416996658074 1109 5 0.021747967712308025 1109 6 -0.2499911465399265 1109 7 -0.02693580403960283 1109 8 -0.1889807937200345 1109 9 0.0792198575836025 1109 10-11 -0.14037914232937965 1109 12-13 -0.017896346634785232 1109 14-15 0.11162291323133644 1109 16-17 0.02714278743678733 1109 18-19 0.051759835493989215 1109 20-21 0.09487740427415048 1109 22-23 -0.05651606392934383 1109 24-25 0.20481995185756574 1109 26-27 0.020286362898303878 1109 28-29 0.01606861930061143 1109 30-31 0.11638137306390206 1109 32-33 0.019551580420653636 1109 34-35 -0.09887444466950512 1109 36-37 0.04829761486148243 1109 38-39 0.06593643385539849 1109 40-41 0.050843234053459696 1109 42-43 0.09492438607156117 1109 44-45 0.17391139437839342 1109 46-47 0.10583530491943804 1109 48-49 -0.006737511294133469 1109 50-51 0.07701898520157613 1109 52-53 0.1591845258332043 1109 54-55 -0.08886651985540794 1109 56-57 0.08837590530096406 1109 58-59 0.20988443447534166 1109 60-61 0.16436844682494467 1109 62-63 -0.12087570155021155 1109 64-65 0.03558305964118347 1109 66-67 -0.06125471907257918 1109 68-69 0.10513347093461078 1109 70-71 0.37723855394294503 1109 72-73 -0.026115286379130964 1109 74-75 0.258111755401643 1109 76 0.527030578768688 1110 1 -0.007083998991468832 1110 2 -0.016046808322066397 1110 3 0.017714303863911596 1110 4 -0.024279716572351617 1110 5 0.05688421640706309 1110 6 -0.2879643371245493 1110 7 -7.381239158092967E-4 1110 8 0.13679260318763653 1110 9 0.04949329852362894 1110 10-11 -0.045860541803506294 1110 12-13 -0.10408890013409433 1110 14-15 -0.33451293288185724 1110 16-17 0.0014617012214372949 1110 18-19 0.07553416337150765 1110 20-21 0.09672833587303131 1110 22-23 -0.06127077224642363 1110 24-25 0.011471511232052478 1110 26-27 0.07232160136496901 1110 28-29 0.06350090382512974 1110 30-31 -0.059858924091614085 1110 32-33 0.03915462871786701 1110 34-35 -0.014500584793047722 1110 36-37 0.16593634823927061 1110 38-39 -0.10538953768712389 1110 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2115 54-55 -0.19838093574342253 2115 56-57 -0.07808776188753797 2115 58-59 -0.2228493191312637 2115 60-61 -0.13632145889437908 2115 62-63 -0.14663879238399602 2115 64-65 -0.07826805691444605 2115 66-67 -0.05923817958771593 2115 68-69 -0.32546615559314773 2115 70-71 0.0016058624857748782 2115 72-73 0.05994566962555581 2115 74-75 0.03600195315941335 2115 76 0.22913557220484648 2116 1 -0.03263494118245802 2116 2 -0.05999373457149915 2116 3 -0.023264408313053764 2116 4 -0.16310708107598515 2116 5 -0.06940721708411246 2116 6 0.05011524572854853 2116 7 -0.03517808614485318 2116 8 0.07859130619959132 2116 9 -0.0964022342274049 2116 10-11 0.07110445062841109 2116 12-13 -0.01314611896958695 2116 14-15 0.03460792029742521 2116 16-17 -0.3876355798250444 2116 18-19 -0.14262436356113284 2116 20-21 -0.09561020022461975 2116 22-23 -0.13924540877038538 2116 24-25 -0.08024717150578198 2116 26-27 0.056298452766803564 2116 28-29 0.11307181463290306 2116 30-31 0.11636533402845117 2116 32-33 -0.05886326388485941 2116 34-35 0.11972691579713057 2116 36-37 -0.1580641794432367 2116 38-39 -0.013745494250521517 2116 40-41 -0.19355602387383897 2116 42-43 -0.027202176175045167 2116 44-45 -0.14521462075888536 2116 46-47 -0.1641016616712534 2116 48-49 -0.1783908328892707 2116 50-51 0.004926194035157039 2116 52-53 0.019016721880113607 2116 54-55 0.0014439424074197404 2116 56-57 0.03657249397633677 2116 58-59 0.042858374606964844 2116 60-61 -0.015939716509464574 2116 62-63 -0.08172263390647316 2116 64-65 -0.08248919086716455 2116 66-67 -0.20227437401946702 2116 68-69 -0.02199103296910465 2116 70-71 0.28968324154708824 2116 72-73 -0.051999616419713846 2116 74-75 0.2709180341493891 2116 76 -0.3265062958648599 2117 1 -0.10721741658854711 2117 2 0.012479980527025702 2117 3 -0.13915670238564815 2117 4 -0.15502018423614317 2117 5 0.01742596207099467 2117 6 -0.07073064567029519 2117 7 -0.08156285532512442 2117 8 -0.8643906002390906 2117 9 -0.1871386093480112 2117 10-11 -0.033045430505140416 2117 12-13 -0.22068095827608403 2117 14-15 -0.03341750419005507 2117 16-17 -0.25235562200024475 2117 18-19 -0.20127575166254985 2117 20-21 -0.08122089522029086 2117 22-23 0.03227339600018553 2117 24-25 -0.24543372716412648 2117 26-27 -0.15206397549058437 2117 28-29 -0.8704679185890285 2117 30-31 -0.24491703096612838 2117 32-33 -0.5591258642250523 2117 34-35 -0.20395883912911472 2117 36-37 -0.07282848569093403 2117 38-39 -0.16666244689293563 2117 40-41 -0.3080229153423062 2117 42-43 -0.15604842566526145 2117 44-45 -0.2700989870863566 2117 46-47 -0.05552437859007853 2117 48-49 -0.2901740284568888 2117 50-51 -0.20712855415698073 2117 52-53 -0.2832929674756173 2117 54-55 -0.0389318545945585 2117 56-57 -0.27314869210187 2117 58-59 -0.13257954130124006 2117 60-61 -0.36110399092682144 2117 62-63 -0.16921529800681867 2117 64-65 -0.4769377798876775 2117 66-67 -0.25423641633392435 2117 68-69 -0.18630722765144725 2117 70-71 -0.2057978442655326 2117 72-73 -0.46984450317331294 2117 74-75 -0.18354567428465884 2117 76 -0.9435633815979045 2118 1 -0.04771663368350687 2118 2 -0.1916893725784945 2118 3 -0.3548086627184617 2118 4 -0.11151522583756446 2118 5 -0.002966411512872469 2118 6 -0.058818208225943636 2118 7 0.011354156740829069 2118 8 -0.15229702006040213 2118 9 -0.24347706994178964 2118 10-11 -0.18115264244214302 2118 12-13 -0.21737433582053 2118 14-15 -0.03955486564657207 2118 16-17 -0.11320422965737009 2118 18-19 -0.19943976259251173 2118 20-21 -0.054685019748248465 2118 22-23 -0.1341200214570577 2118 24-25 -0.1544048978374306 2118 26-27 -0.4314198442081576 2118 28-29 0.030115422419846993 2118 30-31 -0.041297246495275886 2118 32-33 -0.2586653318496275 2118 34-35 -0.15912754748931945 2118 36-37 -0.20030384720884342 2118 38-39 -0.05994481464338719 2118 40-41 -0.10192239647582824 2118 42-43 -0.3610283157515326 2118 44-45 -0.19082601419636092 2118 46-47 -0.307150057875333 2118 48-49 -0.05395776245338624 2118 50-51 -0.08669933798307738 2118 52-53 -0.13332213452606112 2118 54-55 -0.08428815314235294 2118 56-57 -0.2518598154938445 2118 58-59 -0.21898769579450317 2118 60-61 -0.19249387763585446 2118 62-63 -0.19998500111694284 2118 64-65 -0.19499371922022135 2118 66-67 -0.20708404152198057 2118 68-69 -0.1967490317102758 2118 70-71 -0.3167892169590232 2118 72-73 0.09597785413840398 2118 74-75 0.3293707237381227 2118 76 -0.4795555218453629 2119 1 0.0594113528337914 2119 2 -0.15322842724732055 2119 3 -0.15243156568487137 2119 4 -0.043894636079571114 2119 5 -0.15516891638713304 2119 6 -0.002439811389535862 2119 7 0.018128552828201805 2119 8 0.11856646759697753 2119 9 -0.19911413855595583 2119 10-11 0.07078219117207851 2119 12-13 -0.1515239012678009 2119 14-15 -0.6893538368195848 2119 16-17 -0.22912461122660233 2119 18-19 -0.1048868176578921 2119 20-21 -0.1012631235782564 2119 22-23 -0.16817595703971477 2119 24-25 -0.25724683961066575 2119 26-27 0.1351267559262297 2119 28-29 0.07968637733431194 2119 30-31 -0.30669495311435924 2119 32-33 -0.392973517302849 2119 34-35 -0.276553717587241 2119 36-37 -0.2627803020517163 2119 38-39 -0.14519095103456436 2119 40-41 -0.1858367857337342 2119 42-43 -0.23016460778858772 2119 44-45 -0.2766118992188922 2119 46-47 -0.1381999240397036 2119 48-49 -0.11041657082059686 2119 50-51 -0.10994377802141742 2119 52-53 0.028855806277839235 2119 54-55 -0.17412215290001853 2119 56-57 -0.14291210501941976 2119 58-59 -0.19696154552407563 2119 60-61 -0.09413956019756853 2119 62-63 -0.008168251284928374 2119 64-65 -0.3782912528994302 2119 66-67 -0.27817664696572564 2119 68-69 -0.07903095674029004 2119 70-71 -0.10768729258992948 2119 72-73 0.03622988370341318 2119 74-75 0.07988348493790909 2119 76 -1.12602237136565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 3.0 20 4.0 21 5.0 22 16.0 23 36.0 24 67.0 25 147.0 26 270.0 27 490.0 28 862.0 29 1404.0 30 2300.0 31 3662.0 32 5393.0 33 8590.0 34 13549.0 35 22286.0 36 39277.0 37 190666.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.626126188466166 14.520738475165034 9.991073529208244 30.86206180716055 2 24.61353225293051 17.350914098288055 29.18090980804628 28.854643840735157 3 23.074926996692362 16.47349045767192 26.00613089389263 34.445451651743085 4 18.764963948129594 16.980361764258134 26.958979752826718 37.295694534785554 5 20.591430588039913 27.55995958869037 30.67315277412569 21.17545704914403 6 36.38747802981026 29.375008649681 14.76915731347828 19.46835600703046 7 34.37521624202499 22.814744592219437 17.03502774817665 25.77501141757892 8 28.92972307181311 30.515036605449993 15.460093831739485 25.09514649099741 9 26.012358664212464 14.452578988886891 15.683947576013397 43.85111477088725 10-11 31.41408444856554 21.123558963145438 20.22174322210997 27.240613366179055 12-13 29.492125330417814 16.82933833400224 21.551718172633795 32.12681816294615 14-15 27.052050320384186 16.792144705703254 18.241658247643826 37.91414672626873 16-17 30.807568816862034 21.05851336202721 17.895671007653238 30.23824681345752 18-19 31.50525208630306 19.937514704457698 18.31362359356187 30.243609615677375 20-21 28.84236129371549 19.533228614528696 18.567232240474972 33.05717785128085 22-23 29.142851211647315 20.873064201392253 18.48004345599734 31.50404113096309 24-25 31.413565467705553 20.88344381859197 18.07610335330833 29.62688736039415 26-27 30.406742599332937 22.015514067841178 18.801292608328605 28.77645072449728 28-29 30.71916907704444 19.811402355481132 19.11216214346015 30.357266424014284 30-31 30.911883969719195 20.660974023278023 19.10005259006048 29.327089416942304 32-33 28.66106397996042 21.1911994685636 19.639619690825803 30.50811686065018 34-35 29.417565080199843 19.91692846367826 19.864165409579694 30.801341046542202 36-37 29.10807949402826 19.798773821221474 21.85445700762556 29.238689677124707 38-39 26.71436677415337 20.067086925834175 23.593907856678246 29.624638443334213 40-41 26.858643453229448 18.19218207232517 23.889899940490196 31.05927453395519 42-43 28.49464411752495 17.593451153521457 23.779530010933197 30.1323747180204 44-45 25.268486098232696 20.3087590129676 26.04488146477158 28.37787342402812 46-47 26.529436594378396 19.074795521541166 25.06106674785834 29.334701136222098 48-49 26.106121206249917 18.844714006947424 23.392370289383727 31.656794497418932 50-51 24.563710090371867 19.739610003183085 25.19513680335469 30.501543103090356 52-53 22.676695683463194 19.945818398217476 28.175297895013635 29.202188023305702 54-55 22.738800393041505 19.93042196603789 26.228600689206583 31.10217695171402 56-57 23.889726946870198 20.531055814661556 23.935397262548957 31.64381997591929 58-59 23.0588385900328 20.649037463498345 26.050244266991434 30.241879679477425 60-61 24.669928173048977 21.914831781003915 23.394446212823674 30.02079383312343 62-63 22.567536709246163 25.62727486610294 21.2353128416624 30.569875582988498 64-65 22.609747152525014 26.591368310336716 20.342146781626695 30.456737755511575 66-67 22.884115033837553 26.65364601353502 19.617130520226414 30.845108432401013 68-69 22.60992014614501 27.196845980320244 19.814170253401052 30.37906362013369 70-71 23.18858380502927 26.926975933127583 19.61107574352658 30.273364518316566 72-73 23.554811298559308 25.65875970494208 19.5735361279876 31.212892868511013 74-75 23.332860484105346 25.700105180120957 19.85551572857993 31.111518607193766 76 23.65549358539657 25.686092696901337 19.481503522150103 31.176910195551987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.5 14 2.5 15 3.0 16 9.5 17 15.0 18 17.5 19 27.5 20 41.5 21 48.0 22 64.5 23 101.0 24 144.5 25 168.0 26 228.0 27 342.0 28 517.5 29 639.0 30 752.0 31 996.0 32 1402.5 33 1678.0 34 1952.5 35 2444.0 36 2927.5 37 3194.0 38 3650.5 39 4660.0 40 5854.0 41 8540.0 42 10585.0 43 11627.0 44 12383.5 45 11226.0 46 10354.0 47 9775.5 48 8789.0 49 7930.0 50 7479.0 51 7005.0 52 6172.5 53 5492.0 54 5170.0 55 5210.5 56 5284.0 57 5869.0 58 6421.0 59 7031.0 60 8593.0 61 10242.5 62 10940.0 63 11866.5 64 13300.0 65 14291.5 66 15019.0 67 15262.0 68 15028.5 69 14271.5 70 12803.5 71 11859.0 72 10940.0 73 8921.0 74 6707.0 75 5593.0 76 4407.0 77 2443.5 78 1136.5 79 607.0 80 403.5 81 124.5 82 34.0 83 19.0 84 13.5 85 4.5 86 1.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 289028.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.11557504796738 #Duplication Level Percentage of deduplicated Percentage of total 1 66.05344615123384 37.06627114676434 2 21.273571172005195 23.875573592818657 3 6.875554479808822 11.574770802243009 4 2.3572266054385267 5.29108505930204 5 1.0432310539506382 2.9270755250168574 6 0.5754875349654583 1.9376288374514354 7 0.4177079814262066 1.6407946505900086 8 0.29274172163472545 1.314189604005169 9 0.22195898095135544 1.1209820267831532 >10 0.8761477275077268 7.390878794397805 >50 0.0061555195607340225 0.24144894338885126 >100 0.0036933117364404133 0.37512668457838694 >500 6.155519560734022E-4 0.19758053736541192 >1k 0.0018466558682202067 2.2258898300397103 >5k 6.155519560734022E-4 2.8207039652551633 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 8166 2.8253318017631512 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 2329 0.8058042819380822 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 2261 0.7822771496187221 No Hit CACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTG 1854 0.6414603429425523 TruSeq Adapter, Index 2 (95% over 24bp) TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 572 0.19790470127461698 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002421910679934124 0.0 2 0.0 0.0 0.0 0.004497834119877659 0.0 3 0.0 0.0 0.0 0.006919744799811783 0.0 4 0.0 0.0 0.0 0.03702063467899304 0.0 5 0.0 0.0 0.0 0.04013451983890834 0.0 6 0.0 0.0 0.0 0.04048050707889893 0.0 7 0.0 0.0 0.0 0.041518468798870695 0.0 8 0.0 0.0 0.0 0.041864456038861284 0.0 9 0.0 0.0 0.0 0.04843821359868248 0.0 10 0.0 0.0 0.0 0.051898085998588375 0.0 11 0.0 0.0 0.0 0.05259006047856955 0.0 12 0.0 0.0 0.0 0.05293604771856014 0.0 13 0.0 0.0 0.0 0.05328203495855073 0.0 14 0.0 0.0 0.0 0.05570394563848485 0.0 15 0.0 0.0 0.0 0.05639592011846603 0.0 16 0.0 0.0 0.0 0.0574338818384378 0.0 17 0.0 0.0 0.0 0.058471843558409564 0.0 18 0.0 0.0 0.0 0.06089375423834369 0.0 19 0.0 0.0 0.0 0.06504560111823075 0.0 20 0.0 0.0 0.0 0.06954343523810842 0.0 21 0.0 0.0 0.0 0.0733492948780049 0.0 22 0.0 0.0 0.0 0.0826909503577508 0.0 23 0.0 0.0 0.0 0.08753477171761906 0.0 24 0.0 0.0 0.0 0.09583846547739319 0.0 25 0.0 3.4598723999058916E-4 0.0 0.10794801887706382 0.0 26 0.0 3.4598723999058916E-4 0.0 0.12559336811658386 0.0 27 0.0 3.4598723999058916E-4 0.0 0.22108584635398645 0.0 28 0.0 3.4598723999058916E-4 0.0 0.38093195122963863 0.0 29 0.0 3.4598723999058916E-4 0.0 0.5539255712249332 0.0 30 0.0 3.4598723999058916E-4 0.0 0.7151556250605478 0.0 31 0.0 3.4598723999058916E-4 0.0 0.9168661859750612 0.0 32 0.0 3.4598723999058916E-4 0.0 1.1182307596495842 0.0 33 0.0 3.4598723999058916E-4 0.0 1.3787591513624977 0.0 34 0.0 3.4598723999058916E-4 0.0 1.7282062637529927 0.0 35 0.0 3.4598723999058916E-4 0.0 2.2042847059800437 0.0 36 0.0 3.4598723999058916E-4 0.0 2.9471193102398385 0.0 37 0.0 3.4598723999058916E-4 0.0 3.960515936172274 0.0 38 0.0 3.4598723999058916E-4 0.0 4.953499314945264 0.0 39 0.0 3.4598723999058916E-4 0.0 6.024329822716139 0.0 40 0.0 3.4598723999058916E-4 0.0 7.1280291182861175 0.0 41 0.0 3.4598723999058916E-4 0.0 8.28120458917475 0.0 42 0.0 3.4598723999058916E-4 0.0 9.49527381430173 0.0 43 0.0 3.4598723999058916E-4 0.0 10.75881921474736 3.4598723999058916E-4 44 0.0 3.4598723999058916E-4 0.0 11.960778886474667 3.4598723999058916E-4 45 0.0 3.4598723999058916E-4 0.0 13.103228752923592 3.4598723999058916E-4 46 0.0 3.4598723999058916E-4 0.0 14.207274035733562 3.4598723999058916E-4 47 0.0 3.4598723999058916E-4 0.0 15.29886377790387 3.4598723999058916E-4 48 0.0 3.4598723999058916E-4 0.0 16.30949250591638 3.4598723999058916E-4 49 0.0 3.4598723999058916E-4 0.0 17.29382620368961 3.4598723999058916E-4 50 0.0 3.4598723999058916E-4 0.0 18.21103837690466 3.4598723999058916E-4 51 0.0 3.4598723999058916E-4 0.0 19.177726725438365 3.4598723999058916E-4 52 0.0 3.4598723999058916E-4 0.0 20.176937874531188 3.4598723999058916E-4 53 0.0 3.4598723999058916E-4 0.0 21.168191317104224 3.4598723999058916E-4 54 0.0 3.4598723999058916E-4 0.0 22.124846035678203 3.4598723999058916E-4 55 0.0 3.4598723999058916E-4 0.0 23.054167762292927 3.4598723999058916E-4 56 0.0 3.4598723999058916E-4 0.0 23.921903760189323 3.4598723999058916E-4 57 0.0 3.4598723999058916E-4 0.0 24.804171222165326 3.4598723999058916E-4 58 0.0 3.4598723999058916E-4 0.0 25.6691393221418 3.4598723999058916E-4 59 0.0 3.4598723999058916E-4 0.0 26.476327553039845 3.4598723999058916E-4 60 0.0 3.4598723999058916E-4 0.0 27.26275654953845 3.4598723999058916E-4 61 0.0 3.4598723999058916E-4 0.0 28.091741976555905 3.4598723999058916E-4 62 0.0 3.4598723999058916E-4 0.0 28.86502345793487 3.4598723999058916E-4 63 0.0 3.4598723999058916E-4 0.0 29.690549012552417 3.4598723999058916E-4 64 0.0 3.4598723999058916E-4 0.0 30.488395587970714 3.4598723999058916E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTTTC 15 0.0022232148 70.0 41 GAGTAAC 15 0.0022232148 70.0 11 TAACGTC 20 7.918594E-5 70.0 14 GTAACGT 20 7.918594E-5 70.0 13 AGTAACG 15 0.0022232148 70.0 12 TATGCTG 20 0.006946799 52.5 5 GTAACTG 20 0.006946799 52.5 43 GTAGTGA 20 0.006946799 52.5 43 ATATTAA 35 2.083562E-5 50.000004 33 TCTTTAT 100 0.0 48.999996 1 CTTTATA 110 0.0 47.727276 2 ATCGTAT 30 5.8720604E-4 46.666664 41 GGACTAT 45 9.1848124E-5 38.888885 1 CTTAATA 50 1.7073234E-4 35.0 2 GAATCTC 40 0.002416448 35.0 19 TTATCAA 40 0.002416448 35.0 32 TTATATA 60 1.2134036E-5 35.0 2 GGTGAAT 40 0.002416448 35.0 8 TGTACTT 40 0.002416448 35.0 63 CTTGAAA 1695 0.0 33.657818 57 >>END_MODULE