FastQCFastQC Report
Wed 25 May 2016
SRR1780303_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780303_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320950
Sequences flagged as poor quality0
Sequence length50
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA300.00571720129.36267334
TACGTTC300.005727809429.35153813
TCTTATG500.0025588522.03203417
CTGCTAT500.002575248222.0078122
CTTACAA654.975048E-420.2818372
ATGACAA600.007436218.3231730
GATAGTC600.007436218.3231726
CCATAGG600.00746712118.3099923
TACAGGA1002.510629E-517.5775932
TTCTAAC750.00130202817.577592
TTGTACA800.002005485116.4789922
ACGAGAG1401.5743062E-615.72582624
GCGATTT1004.89116E-415.42183915
TGTAGAA1452.3163302E-615.1530962
TACAGGC1452.3163302E-615.1530962
AGGATAG900.004338073514.67743924
AAGTAAA900.004386406414.65298533
GACAGAG1057.228901E-414.6479942
GATTAAG1057.228901E-414.6479942
TCTAACC900.004396348314.6479933
Produced by FastQC (version 0.11.5)