FastQCFastQC Report
Wed 25 May 2016
SRR1780303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320950
Sequences flagged as poor quality0
Sequence length50
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT190021.4385101631401644No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC57020.4316590332715091No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG53320.403648889057118No Hit
CACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTTCTG33890.2565577803853287RNA PCR Primer, Index 31 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT29500.030.35089744
ACGATTT300.005745662429.33285521
CTTTATA1700.028.4755132
TATGCCG32900.027.08059943
TCTTCTG5350.026.31633244
GTCTTCT5700.025.0863643
TCTTTAT1950.024.8756661
TTTATAC2150.024.5623863
GTATAGA450.001380316624.4987621
CCGTCTT5850.024.4431241
GATACTA500.002547517222.0488851
GTTAATA602.869614E-422.0038052
GCTTATA602.869614E-422.0038053
ATAGGAA500.00258194321.99797433
CGTTATC40550.021.75385333
TCGTTAT40650.021.7003432
GTTATCT40550.021.69960434
CTCGTAT41000.021.67687439
TCGTATG41000.021.67687440
GTATGCC41200.021.62504242
Produced by FastQC (version 0.11.5)