FastQCFastQC Report
Wed 25 May 2016
SRR1780302_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780302_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145822
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTG150.002220999470.067
CTCTGAA150.002220999470.021
GTCTGCA200.00693993152.557
TATGGAC200.00693993152.567
CCGAGTT200.00693993152.560
CCAGTAT200.00693993152.531
CGAAGAC200.00693993152.521
AGACGTA350.001252166540.05
GCCTAAA350.001252166540.01
TCGCATA400.002412535335.040
AGAAATC501.7031828E-435.047
GAAATCG552.9807424E-431.81818448
GCCTTAA450.00429625231.11111360
CGACGAT450.00429625231.11111319
ATAGACG450.00429625231.1111133
TTATCAA450.00429625231.11111332
CATCAAT450.00429625231.11111312
CTACTGA604.96323E-429.16666633
TCACATC500.007190061327.99999844
CTTCAGC500.007190061327.99999817