##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780302_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 145822 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.053633882404576 34.0 31.0 34.0 12.0 34.0 2 30.24431155792679 34.0 31.0 34.0 12.0 34.0 3 30.129472919038278 34.0 31.0 34.0 11.0 34.0 4 29.876362963064558 34.0 31.0 34.0 11.0 34.0 5 29.74915307703913 34.0 31.0 34.0 11.0 34.0 6 32.97959155682956 37.0 34.0 38.0 21.0 38.0 7 33.24332405261209 37.0 34.0 38.0 21.0 38.0 8 33.22205154229129 37.0 34.0 38.0 21.0 38.0 9 33.69244009820192 38.0 34.0 38.0 21.0 38.0 10-11 33.41008901263184 38.0 34.0 38.0 21.0 38.0 12-13 33.50653193619618 38.0 34.0 38.0 21.0 38.0 14-15 33.51248782762546 38.0 34.0 38.0 21.0 38.0 16-17 33.607295195512336 38.0 34.0 38.0 21.0 38.0 18-19 33.62331129733511 38.0 34.0 38.0 21.0 38.0 20-21 33.64772462317072 38.0 34.0 38.0 21.0 38.0 22-23 33.676742878303685 38.0 34.0 38.0 21.0 38.0 24-25 33.70078931848418 38.0 34.0 38.0 21.0 38.0 26-27 33.69605752218458 38.0 34.0 38.0 21.0 38.0 28-29 33.823311983102684 38.0 34.0 38.0 21.0 38.0 30-31 33.84807847924182 38.0 34.0 38.0 21.0 38.0 32-33 33.91068220158824 38.0 34.0 38.0 21.0 38.0 34-35 33.82676139402834 38.0 34.0 38.0 21.0 38.0 36-37 33.77050445063159 38.0 34.0 38.0 21.0 38.0 38-39 33.79469490200381 38.0 34.0 38.0 21.0 38.0 40-41 33.87304384797904 38.0 34.0 38.0 21.0 38.0 42-43 33.93785231309404 38.0 34.0 38.0 21.5 38.0 44-45 33.986061773943575 38.0 34.0 38.0 22.0 38.0 46-47 33.92644114056864 38.0 34.0 38.0 21.5 38.0 48-49 33.89571532416234 38.0 34.0 38.0 21.0 38.0 50-51 33.828750120009325 38.0 34.0 38.0 21.0 38.0 52-53 33.865311818518464 38.0 34.0 38.0 21.0 38.0 54-55 33.851061568213304 38.0 34.0 38.0 21.0 38.0 56-57 33.79780828681543 38.0 34.0 38.0 21.0 38.0 58-59 33.867211394714104 38.0 34.0 38.0 21.5 38.0 60-61 33.88050842808356 38.0 34.0 38.0 22.0 38.0 62-63 33.76510403094183 38.0 34.0 38.0 21.5 38.0 64-65 33.734227345668 38.0 34.0 38.0 21.0 38.0 66-67 33.58449685232681 38.0 34.0 38.0 20.5 38.0 68-69 33.50994362990495 37.5 34.0 38.0 20.0 38.0 70-71 33.514298254035744 37.5 34.0 38.0 20.0 38.0 72-73 33.58526491201602 38.0 34.0 38.0 20.0 38.0 74-75 33.60684258890977 38.0 34.0 38.0 20.0 38.0 76 31.820850077491738 36.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5602695847472603 1101 2 0.36083610374500097 1101 3 0.3657181441080013 1101 4 0.6356016074820836 1101 5 0.22267797761922736 1101 6 0.7426471197336681 1101 7 -0.3069507466474306 1101 8 0.045893181521400095 1101 9 -0.33915414025089774 1101 10-11 -0.2497654606951656 1101 12-13 0.03574021105867331 1101 14-15 -0.02978740718165085 1101 16-17 -0.008616300207485494 1101 18-19 -0.19326542008552394 1101 20-21 0.3413738950647769 1101 22-23 -0.06456937905429072 1101 24-25 0.1430403956258388 1101 26-27 -0.23949558525582404 1101 28-29 -0.6003272657482484 1101 30-31 -0.3387054255837043 1101 32-33 -0.031179446183223547 1101 34-35 -0.4524333799812865 1101 36-37 -0.40214738175083653 1101 38-39 0.05136347027131194 1101 40-41 -0.30669087138538487 1101 42-43 -0.20174057729758488 1101 44-45 0.13181387672996436 1101 46-47 -0.09210856398691192 1101 48-49 -0.5791180347253828 1101 50-51 -0.4195490573583385 1101 52-53 -0.30048210125909236 1101 54-55 -0.3698266141048592 1101 56-57 -0.34121981557007075 1101 58-59 -0.0577097877300119 1101 60-61 -0.40305889829172514 1101 62-63 -0.056519864898866956 1101 64-65 -0.4225266015169282 1101 66-67 -0.4738350733355077 1101 68-69 -0.512761271402546 1101 70-71 -0.4255753441449741 1101 72-73 -0.16794418965112357 1101 74-75 -0.5717028022532773 1101 76 -0.914191801494038 1102 1 0.1468081644064867 1102 2 0.09176739620351171 1102 3 0.4455616388194983 1102 4 0.5376813834117158 1102 5 -0.4106770584359545 1102 6 0.22570671213198779 1102 7 -0.08783720046363186 1102 8 0.06484825688717422 1102 9 0.20207284155566896 1102 10-11 0.24827208091878816 1102 12-13 0.04355933320404404 1102 14-15 0.3323184120142031 1102 16-17 -0.11351108295504986 1102 18-19 -0.47485823607510724 1102 20-21 -0.04248173679153666 1102 22-23 0.04396777559602327 1102 24-25 -0.21895179139429644 1102 26-27 -0.161602264370849 1102 28-29 0.012346714326199049 1102 30-31 0.33361009104344674 1102 32-33 0.24529377961926002 1102 34-35 0.10992918650889294 1102 36-37 -0.16242034466375088 1102 38-39 0.14360515669564222 1102 40-41 0.3748902742726443 1102 42-43 -0.10897065481315593 1102 44-45 0.5363874182456243 1102 46-47 -0.30208854015486963 1102 48-49 0.17041822315209743 1102 50-51 0.39605356170975625 1102 52-53 0.43805640469277307 1102 54-55 -0.04242160446148091 1102 56-57 0.22623470084823794 1102 58-59 -0.08207314043200853 1102 60-61 0.3794583308030255 1102 62-63 -0.176721208829683 1102 64-65 0.16813037672711317 1102 66-67 0.434893720443597 1102 68-69 0.22531656356667895 1102 70-71 0.44867069101343304 1102 72-73 0.46367258122955235 1102 74-75 -0.16277438438170577 1102 76 0.32290019757233424 1103 1 0.7402064653444498 1103 2 0.8867185525914998 1103 3 0.6038774332577539 1103 4 0.6112622067670408 1103 5 0.36432397326375465 1103 6 -0.7386849092872794 1103 7 0.26860620638567667 1103 8 0.3072564208743671 1103 9 0.4199265524321447 1103 10-11 0.35986956475057497 1103 12-13 0.5937529979782781 1103 14-15 -2.2493411220381176E-4 1103 16-17 0.5221485858398651 1103 18-19 0.2403602189693217 1103 20-21 0.5334513619037935 1103 22-23 0.5612472718907568 1103 24-25 0.44266249440421035 1103 26-27 0.6125144532912614 1103 28-29 0.48000440225766994 1103 30-31 0.25861141751450845 1103 32-33 0.5306560241262019 1103 34-35 0.2938429246222398 1103 36-37 0.3585054731793491 1103 38-39 0.1346676894889569 1103 40-41 0.2569787882123791 1103 42-43 0.1975915448885388 1103 44-45 0.33206076443099164 1103 46-47 0.39698528827641866 1103 48-49 0.34093460359578387 1103 50-51 0.03555115342786053 1103 52-53 0.4642379956326863 1103 54-55 0.5959322744346522 1103 56-57 0.5425449889577578 1103 58-59 0.18251951504513642 1103 60-61 0.5426054276000372 1103 62-63 0.027599549558040337 1103 64-65 0.5223865801900942 1103 66-67 0.18252228496609035 1103 68-69 1.0324034562640136 1103 70-71 0.6052827070725826 1103 72-73 0.47095343347247365 1103 74-75 0.5899212995762468 1103 76 0.8417154525790274 1104 1 0.49570487996356505 1104 2 0.0150279651602645 1104 3 0.032412048340720645 1104 4 0.59591258266477 1104 5 -0.294096471987384 1104 6 -0.03190754606139734 1104 7 0.1040291053358473 1104 8 -0.7316498343775564 1104 9 -0.22660073516603063 1104 10-11 0.030135550207646133 1104 12-13 -0.17974201059286088 1104 14-15 0.02458655681807187 1104 16-17 -0.12212107456370802 1104 18-19 -0.08108555876363965 1104 20-21 -0.733830970342737 1104 22-23 -0.16214017727062213 1104 24-25 -0.37210563663900587 1104 26-27 -0.12374371170039211 1104 28-29 -0.49791790847698536 1104 30-31 -0.18660891121394485 1104 32-33 -0.31902409867079484 1104 34-35 -0.077844861782701 1104 36-37 -0.12446428694075706 1104 38-39 0.10855652235731839 1104 40-41 -0.690588800552149 1104 42-43 -0.12079831315544709 1104 44-45 0.004447126794161704 1104 46-47 -0.25977134735487084 1104 48-49 -0.22367947100114804 1104 50-51 -0.42922033884690336 1104 52-53 -0.25899539708240127 1104 54-55 -0.43007805575032165 1104 56-57 -0.22784928350367295 1104 58-59 -0.3160911759120282 1104 60-61 -0.10904493709358576 1104 62-63 -0.3732441195623224 1104 64-65 -0.15103616190260993 1104 66-67 0.11910144757126773 1104 68-69 -0.40352306721418785 1104 70-71 -0.13607892783044662 1104 72-73 -0.21958077826270284 1104 74-75 -0.497145801809701 1104 76 0.0876175511079289 1105 1 0.4005518245797255 1105 2 0.793286545190945 1105 3 0.18497518225559517 1105 4 0.6516055102925833 1105 5 0.7853847194863874 1105 6 1.2089972779878764 1105 7 0.36990130351796324 1105 8 0.18463333525405545 1105 9 0.5829443343213399 1105 10-11 0.5281551028325921 1105 12-13 -0.2630925154352255 1105 14-15 0.27570270490516435 1105 16-17 0.5706119428138621 1105 18-19 0.5221209343563018 1105 20-21 0.35678880665660273 1105 22-23 0.7353401899707208 1105 24-25 0.42457224874689103 1105 26-27 0.16249389619392218 1105 28-29 0.1294031071605346 1105 30-31 0.2671940056530602 1105 32-33 0.07272098455378284 1105 34-35 0.416620188472578 1105 36-37 0.3202949685026084 1105 38-39 0.26227587121369567 1105 40-41 0.3068297492566785 1105 42-43 0.4699473882434546 1105 44-45 0.4098950742264478 1105 46-47 0.5217154763738563 1105 48-49 0.13947504968479052 1105 50-51 0.6304735503854104 1105 52-53 0.3124495281642936 1105 54-55 0.38630127433187056 1105 56-57 0.548686421787437 1105 58-59 0.5684533211917113 1105 60-61 0.7262573959421061 1105 62-63 0.6280723627968072 1105 64-65 0.13431997519472105 1105 66-67 0.500463491667702 1105 68-69 0.46320312735045377 1105 70-71 0.4103757279380318 1105 72-73 0.3184197712255852 1105 74-75 0.2380351858954981 1105 76 0.2956675545422307 1106 1 0.0667949273569306 1106 2 -0.12004312488712898 1106 3 0.3380992521321886 1106 4 0.7499410186837245 1106 5 0.16798883606948678 1106 6 0.47520145867794383 1106 7 -0.5334116529579873 1106 8 -0.04918537940125134 1106 9 0.27339926483396937 1106 10-11 -0.01725781472126897 1106 12-13 0.31077931642135326 1106 14-15 0.002074708476833109 1106 16-17 0.2202959870476633 1106 18-19 0.18431728483825083 1106 20-21 0.6299131054866436 1106 22-23 0.4065802018763023 1106 24-25 0.3139133207069662 1106 26-27 0.4663036816645416 1106 28-29 0.24734275503012526 1106 30-31 -0.38447620979214037 1106 32-33 0.33855883971783385 1106 34-35 0.05604139414147369 1106 36-37 0.35776320121089356 1106 38-39 0.3751442000824312 1106 40-41 0.4622548013435903 1106 42-43 0.29863296646540505 1106 44-45 0.7034992594955867 1106 46-47 0.4049205957730848 1106 48-49 0.25262384653439796 1106 50-51 0.44163274172181133 1106 52-53 0.5004832759033775 1106 54-55 -0.06396431167449634 1106 56-57 0.27452038474277174 1106 58-59 0.3142405776424724 1106 60-61 0.23574179276423735 1106 62-63 0.3743862121912329 1106 64-65 0.5811913262490478 1106 66-67 0.2529877034954424 1106 68-69 0.19600299913652464 1106 70-71 0.14946609586623083 1106 72-73 0.3728362833486685 1106 74-75 0.01825704179219656 1106 76 0.45728579755342835 1107 1 0.4633537569468764 1107 2 0.33434934712331454 1107 3 0.197052476941586 1107 4 -0.432935552509349 1107 5 0.2902156373607845 1107 6 -0.02288677998313915 1107 7 0.541398888059021 1107 8 0.03729034008497223 1107 9 0.7616882956739985 1107 10-11 0.18878312215627346 1107 12-13 0.5026079676565658 1107 14-15 0.3300505309921178 1107 16-17 0.7107085611713444 1107 18-19 0.873570209718622 1107 20-21 0.8507501593136055 1107 22-23 0.5227798034972935 1107 24-25 0.7035577559932662 1107 26-27 0.5006581474016514 1107 28-29 0.7948620443586023 1107 30-31 0.6995611554115087 1107 32-33 0.7864891004958139 1107 34-35 0.8671032802600251 1107 36-37 0.8275413453142022 1107 38-39 0.3975415716967916 1107 40-41 0.8068297492566785 1107 42-43 0.9409618809970794 1107 44-45 0.6780110162554323 1107 46-47 0.23669132178449104 1107 48-49 0.8955136970277948 1107 50-51 0.6026957726076319 1107 52-53 0.7327393832367548 1107 54-55 0.6121466849598889 1107 56-57 0.7129376295169152 1107 58-59 0.6674871376168312 1107 60-61 0.4267404877295462 1107 62-63 0.3780723627968072 1107 64-65 0.5425325355811879 1107 66-67 0.783072187319874 1107 68-69 0.7796282481234016 1107 70-71 0.6181051965370656 1107 72-73 0.7423328147038433 1107 74-75 0.7199192438665136 1107 76 0.3053293902910248 1108 1 -0.659199929713445 1108 2 -1.057907079407947 1108 3 -0.47373986411416524 1108 4 0.31994713023686927 1108 5 0.6216448132837726 1108 6 0.13995394077570467 1108 7 -0.102241578812162 1108 8 -0.6319680963902172 1108 9 -0.8112515117945307 1108 10-11 -0.3682887068927059 1108 12-13 -0.2650760127376728 1108 14-15 -0.32103907019268973 1108 16-17 0.06452921775302656 1108 18-19 -0.27375515436332165 1108 20-21 -0.03471829867151399 1108 22-23 0.03999656008250696 1108 24-25 -0.39428596205038247 1108 26-27 -0.21695354553533264 1108 28-29 0.08779374152705799 1108 30-31 -0.34016168389876356 1108 32-33 -0.28389419933844806 1108 34-35 0.08891117193462605 1108 36-37 -0.5241195030937078 1108 38-39 -0.20165092451253486 1108 40-41 0.0161460156976716 1108 42-43 -0.243591523565847 1108 44-45 -0.5658357343352094 1108 46-47 -0.28504780865029034 1108 48-49 -0.3785439213649582 1108 50-51 -0.07783774408900968 1108 52-53 -0.2193481374797699 1108 54-55 -0.24036045563040176 1108 56-57 -0.44231290823124425 1108 58-59 -0.28454748983829603 1108 60-61 -0.34171926049852885 1108 62-63 -0.11320906461600799 1108 64-65 0.24084700365128953 1108 66-67 0.35244169964400385 1108 68-69 0.14546449058611444 1108 70-71 0.1314254828659145 1108 72-73 0.12334942318792486 1108 74-75 0.18868577301069678 1108 76 -0.6199676935271654 1109 1 0.3336685995255877 1109 2 0.025860734286872855 1109 3 0.0800038583937166 1109 4 0.10850801142296973 1109 5 -0.5187013327256018 1109 6 -0.10956962903480161 1109 7 0.08960097749049822 1109 8 -0.43440238498106254 1109 9 0.3184813277294012 1109 10-11 0.39801779053636466 1109 12-13 -0.25490018303000284 1109 14-15 0.23499091794150218 1109 16-17 -0.06280349232571325 1109 18-19 0.519788767106597 1109 20-21 0.22807340245393704 1109 22-23 0.19774589188167369 1109 24-25 -0.12173792949732132 1109 26-27 0.301144808832845 1109 28-29 -0.015351896290120237 1109 30-31 0.07755073205176899 1109 32-33 -0.14940604716844774 1109 34-35 -0.00785209919310148 1109 36-37 0.02642404682059407 1109 38-39 -0.2257301257878055 1109 40-41 -0.3571834822228226 1109 42-43 0.00885055570734039 1109 44-45 -0.39281666514196445 1109 46-47 0.18005892369781407 1109 48-49 0.11915782734358515 1109 50-51 0.4191208933805797 1109 52-53 -0.1736348106186938 1109 54-55 0.1400256026439095 1109 56-57 0.08858314009495416 1109 58-59 0.35485178862466427 1109 60-61 0.3617194268314208 1109 62-63 0.11958850948537503 1109 64-65 0.1291975364141038 1109 66-67 0.32419448331474143 1109 68-69 0.29142592071568174 1109 70-71 0.5436364785151184 1109 72-73 0.1744203423685846 1109 74-75 0.477558281490559 1109 76 -0.537344510853643 1110 1 -0.3374060573207487 1110 2 0.014418293232782986 1110 3 0.5511048197022461 1110 4 -0.008283800715343403 1110 5 -0.34778211133768266 1110 6 0.6905881977383572 1110 7 0.021113723198084244 1110 8 0.4999679818466163 1110 9 0.5602671615808532 1110 10-11 0.37048777953631173 1110 12-13 0.881219430533541 1110 14-15 0.17859773797115253 1110 16-17 0.27045013051612443 1110 18-19 0.23756580091978918 1110 20-21 0.2634887738251237 1110 22-23 0.1487119830385879 1110 24-25 0.5502992315338204 1110 26-27 0.006421244817346405 1110 28-29 0.42656904684441344 1110 30-31 0.5554250923750672 1110 32-33 0.3917843493115356 1110 34-35 0.549001408865621 1110 36-37 0.43752094289406074 1110 38-39 0.4011530345737597 1110 40-41 0.4690934798509261 1110 42-43 0.584810668577397 1110 44-45 0.2646274976587506 1110 46-47 -0.07671447531696174 1110 48-49 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0.5558442551175489 2116 14-15 0.21369167502187736 2116 16-17 0.04111341774011379 2116 18-19 0.2208619031049679 2116 20-21 0.41951592392392456 2116 22-23 0.27912839911098786 2116 24-25 -0.15438974518239235 2116 26-27 0.21611494702543865 2116 28-29 -0.4813232405800463 2116 30-31 0.16961373973881422 2116 32-33 -0.11819646232748937 2116 34-35 -0.24034189095967662 2116 36-37 -0.3031820098223861 2116 38-39 0.06213479131116628 2116 40-41 -0.16300907764255612 2116 42-43 -0.16165237234822882 2116 44-45 0.3578772041054634 2116 46-47 0.20905248633495432 2116 48-49 0.11987349353071863 2116 50-51 -0.06981715971676294 2116 52-53 0.15545538511781842 2116 54-55 -0.3893603049080028 2116 56-57 0.16220230396434943 2116 58-59 0.1980010104739094 2116 60-61 -0.021412648091917674 2116 62-63 -0.06710954477447473 2116 64-65 -0.5764020973973061 2116 66-67 0.22340180127396536 2116 68-69 0.22053500713327878 2116 70-71 0.3861463234662281 2116 72-73 0.01208400105756624 2116 74-75 0.2938900018503503 2116 76 0.32176733650965517 2117 1 0.036439913255225775 2117 2 -1.2567860667648354 2117 3 -0.3634970029197113 2117 4 -0.8130369128721142 2117 5 -0.6319413822889004 2117 6 -0.5631493685252664 2117 7 -0.0037835309564187014 2117 8 -0.23135907753750473 2117 9 -0.6658034799106005 2117 10-11 -0.04759102205996868 2117 12-13 -0.4284699494176252 2117 14-15 -0.2590176649712461 2117 16-17 -0.5070976686380888 2117 18-19 -0.33038047342650856 2117 20-21 -0.7040792766841406 2117 22-23 -0.22139642127375936 2117 24-25 -0.7098487820464996 2117 26-27 0.03278523314303072 2117 28-29 -0.5423019401113294 2117 30-31 0.285761920059862 2117 32-33 -0.16353460953056498 2117 34-35 0.05559798996038978 2117 36-37 -0.27610851687126825 2117 38-39 -0.15316824721789146 2117 40-41 -0.18698341573546884 2117 42-43 -0.7087595261929849 2117 44-45 -0.8996467295138046 2117 46-47 -0.7983402421881323 2117 48-49 -0.6066498428791505 2117 50-51 -0.28179935201819717 2117 52-53 -0.7423132233842722 2117 54-55 -0.2681991850498875 2117 56-57 -1.0305198850795776 2117 58-59 -0.32248055747222537 2117 60-61 -0.28762778825515767 2117 62-63 -0.29336594667831406 2117 64-65 -0.39444325055436735 2117 66-67 -0.6121665430082146 2117 68-69 -0.9222478077137097 2117 70-71 -0.07231595280646275 2117 72-73 -1.530050932073074 2117 74-75 0.004017225550519754 2117 76 -0.554736997782836 2118 1 -0.379818693525138 2118 2 -0.06719300096869674 2118 3 0.37694876732850346 2118 4 -0.7919303670286943 2118 5 -0.42444996561754067 2118 6 0.21227698370076808 2118 7 0.3287055405431758 2118 8 0.15383069376134983 2118 9 0.3431269341529273 2118 10-11 -0.6009389892650816 2118 12-13 -0.15649099967934177 2118 14-15 -0.15263129323337665 2118 16-17 0.3229055207955085 2118 18-19 0.08160992074996187 2118 20-21 -0.0449640630858994 2118 22-23 0.3032278822102299 2118 24-25 0.37878932980881075 2118 26-27 -0.11467447680612253 2118 28-29 0.13875470047702976 2118 30-31 0.10183478923679701 2118 32-33 0.22716631540518506 2118 34-35 -0.17348307793702133 2118 36-37 0.09987880043985342 2118 38-39 -0.306664429278257 2118 40-41 -0.06827551156856515 2118 42-43 0.08869267920653101 2118 44-45 0.16605560252499174 2118 46-47 0.03605310878089796 2118 48-49 0.25945023029021286 2118 50-51 0.2883869484264494 2118 52-53 0.061696082257270746 2118 54-55 0.09675644430040364 2118 56-57 -0.003977231095596778 2118 58-59 0.08705678704288289 2118 60-61 0.05129174421139737 2118 62-63 -0.024323151984027902 2118 64-65 -0.1922988254966853 2118 66-67 -0.2343124689758298 2118 68-69 -0.667154094650158 2118 70-71 0.30851648936160103 2118 72-73 0.4222623453513634 2118 74-75 0.08758976640341132 2118 76 -0.10939826117376583 2119 1 -0.30336609413675575 2119 2 -0.027437649286738974 2119 3 -0.32947325102012215 2119 4 -0.15890284409291766 2119 5 0.642828321844231 2119 6 -0.22808998877629705 2119 7 0.6834456017052588 2119 8 0.857194608134435 2119 9 0.9455734173333497 2119 10-11 0.7797949556890416 2119 12-13 0.3939677159169861 2119 14-15 0.23880601563512016 2119 16-17 0.15165169816553714 2119 18-19 -0.2716010884243545 2119 20-21 -0.15964296216846918 2119 22-23 -0.14631340219344224 2119 24-25 0.28551715855498827 2119 26-27 0.37309310729173717 2119 28-29 0.40175164237304983 2119 30-31 0.48409778617870103 2119 32-33 0.5803773245794872 2119 34-35 0.21030897099264223 2119 36-37 0.11211121634199372 2119 38-39 0.2957820539021654 2119 40-41 -0.008642484045630283 2119 42-43 0.16208717461937994 2119 44-45 -0.18409730267379132 2119 46-47 -0.08627105024051218 2119 48-49 0.045382952002753996 2119 50-51 0.4886927588240013 2119 52-53 0.2176593850095685 2119 54-55 -0.004160986892252083 2119 56-57 0.2529035028493567 2119 58-59 -0.0582031517950341 2119 60-61 -0.06644525884670571 2119 62-63 -0.10383385534793632 2119 64-65 -0.2626352169339867 2119 66-67 -0.4346182793733817 2119 68-69 -0.6319858989315037 2119 70-71 -0.2862847338799881 2119 72-73 -0.1541902540370117 2119 74-75 -0.4858047290094305 2119 76 -0.1950251724887515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 7.0 13 120.0 14 640.0 15 1376.0 16 1848.0 17 2180.0 18 2231.0 19 2100.0 20 1796.0 21 1425.0 22 1299.0 23 1296.0 24 1304.0 25 1530.0 26 1725.0 27 2031.0 28 2418.0 29 2904.0 30 3488.0 31 4139.0 32 4979.0 33 6234.0 34 8546.0 35 12399.0 36 21857.0 37 55948.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.572300475922695 17.823785162732648 9.984090192152076 28.619824169192576 2 23.103509758472658 19.335902675865096 31.14619193263019 26.41439563303205 3 19.471684656636175 22.625529755455283 27.962858827886052 29.939926760022495 4 15.014881156478447 17.469929091632263 31.946482698083965 35.568707053805326 5 15.1966095650862 31.064585590651618 32.4621799179822 21.276624926279986 6 28.601994212121628 34.6250908642043 18.193413888165022 18.579501035509043 7 29.205469682215302 27.28120585371206 21.16278750805777 22.350536956014867 8 25.77594601637613 30.55711758170921 20.530509799618713 23.136426602295952 9 24.6128842012865 17.78538217827214 20.592228881787385 37.00950473865397 10-11 25.943273305811193 22.983843315823403 25.693996790607727 25.378886587757677 12-13 25.265392053325286 21.2543374799413 25.079891923029447 28.40037854370397 14-15 24.07147069715132 22.124576538519563 22.98967234025044 30.81428042407867 16-17 24.811756799385552 24.709920313807242 23.870197912523487 26.608124974283715 18-19 23.999122217498044 24.14450494438425 25.02468763286747 26.83168520525024 20-21 23.62160716489967 24.388638202740324 25.327454019283785 26.66230061307622 22-23 25.074062898602406 24.099244284127224 24.579281589883557 26.247411227386813 24-25 24.205881142763094 24.95611087490228 24.36395056987286 26.47405741246177 26-27 23.370616230747075 25.08777825019544 25.356256257629163 26.185349261428314 28-29 24.000836636447175 24.487388734210203 26.210036894295786 25.301737735046835 30-31 23.860254282618534 24.16404932040433 26.096885243653222 25.878811153323916 32-33 23.259864766633292 25.15566924058098 25.86578156931053 25.718684423475196 34-35 23.95831904650876 24.409211230129884 25.916871253994593 25.715598469366764 36-37 24.140733222696163 23.935345832590418 25.930243721797808 25.99367722291561 38-39 23.179972843603846 24.18119350989563 27.252746499156505 25.38608714734402 40-41 23.173458051597155 23.383645814760463 27.530825252705355 25.912070880937033 42-43 22.302190341649407 23.188544938349494 27.403272482890102 27.105992237111 44-45 21.673684354898437 22.922467117444555 28.82829751340676 26.575551014250248 46-47 21.82455322242186 22.87206320034014 28.845441702898057 26.457941874339948 48-49 21.315370794530317 23.29758198351415 28.954135864272878 26.432911357682652 50-51 21.11924126674987 24.239140870376215 28.086296992223396 26.55532087065052 52-53 20.349467158590613 24.35880731302547 27.952572314191276 27.339153214192645 54-55 20.132764603420608 24.229197240471258 27.90491146740547 27.733126688702665 56-57 20.243516067534394 24.277886738626545 27.547969442196653 27.93062775164241 58-59 20.479420114934648 24.020381012467254 27.50785203878701 27.992346833811084 60-61 20.539767661944015 23.623664467638626 27.787302327495166 28.049265542922193 62-63 20.59428618452634 23.75464607535214 27.122107775232816 28.5289599648887 64-65 21.269081482903815 23.93946043806833 26.065339934989236 28.72611814403862 66-67 21.13741410761065 24.271029062830028 25.145382726886208 29.446174102673123 68-69 21.212505657582533 24.638943369313274 25.13063872392369 29.017912249180505 70-71 21.687742590281303 24.879304905981265 24.718492408552894 28.714460095184542 72-73 22.01245353924648 25.23041790676304 24.21136728340031 28.54576127059017 74-75 21.657225932986794 26.110600595246257 24.45275747143778 27.779416000329167 76 22.3985406865905 25.442662972665303 24.033410596480643 28.125385744263554 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.0 12 1.0 13 1.5 14 1.5 15 4.0 16 8.5 17 10.0 18 12.5 19 15.0 20 25.0 21 35.0 22 43.5 23 74.5 24 121.0 25 145.0 26 213.5 27 316.5 28 465.0 29 579.0 30 730.5 31 1033.0 32 1417.0 33 1650.0 34 1960.0 35 2518.5 36 3106.5 37 3446.0 38 3959.5 39 5116.5 40 6255.0 41 7123.0 42 7496.0 43 7762.5 44 8437.5 45 8851.0 46 8856.0 47 8842.5 48 8618.5 49 8074.5 50 7741.0 51 7240.5 52 6103.5 53 4961.0 54 4455.0 55 4175.0 56 3555.0 57 3050.5 58 2886.0 59 2647.0 60 2390.0 61 2296.0 62 2220.0 63 2207.0 64 2227.5 65 2252.0 66 2294.0 67 2345.0 68 2391.5 69 2359.5 70 2234.5 71 2188.0 72 2102.0 73 1806.5 74 1483.0 75 1369.0 76 1149.5 77 762.0 78 473.0 79 352.0 80 250.5 81 115.0 82 60.0 83 39.0 84 29.0 85 17.5 86 11.5 87 7.0 88 6.5 89 6.0 90 3.5 91 1.5 92 2.0 93 1.5 94 2.5 95 5.5 96 7.0 97 5.0 98 4.5 99 17.0 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 145822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.8035824498361 #Duplication Level Percentage of deduplicated Percentage of total 1 77.38115378108611 47.050513639917156 2 11.594202898550725 14.099381437643155 3 4.114363051937066 7.505040391710442 4 2.0131957367619693 4.896380518714597 5 1.201150397563864 3.6517123616463905 6 0.8887385101223708 3.2423091165942037 7 0.6327186601251904 2.6930092852930283 8 0.4376022105678678 2.128622567239511 9 0.3327130209214459 1.820712923975806 >10 1.4019060508656178 12.838940626242954 >50 0.0022556814977725144 0.07337713102275377 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.857675796519044E-4 0.0 2 0.0 0.0 0.0 0.0013715351593038088 0.0 3 0.0 0.0 0.0 0.0013715351593038088 0.0 4 0.0 0.0 0.0 0.006171908216867139 0.0 5 0.0 0.0 0.0 0.008229210955822852 0.0 6 0.0 0.0 0.0 0.008229210955822852 0.0 7 0.0 0.0 0.0 0.009600746115126662 0.0 8 0.0 0.0 0.0 0.011658048854082375 0.0 9 0.0 0.0 0.0 0.014401119172689991 0.0 10 0.0 0.0 0.0 0.015086886752341896 0.0 11 0.0 0.0 0.0 0.016458421911645704 0.0 12 0.0 0.0 0.0 0.01714418949129761 0.0 13 0.0 0.0 0.0 0.017829957070949514 0.0 14 0.0 0.0 0.0 0.019201492230253324 0.0 15 0.0 0.0 0.0 0.019887259809905227 0.0 16 0.0 0.0 0.0 0.02057302738955713 0.0 17 0.0 0.0 0.0 0.02194456254886094 0.0 18 0.0 0.0 0.0 0.022630330128512843 0.0 19 0.0 0.0 0.0 0.024001865287816653 0.0 20 0.0 0.0 0.0 0.02674493560642427 0.0 21 0.0 0.0 0.0 0.027430703186076176 0.0 22 0.0 0.0 0.0 0.03497414656224712 0.0 23 0.0 0.0 0.0 0.03565991414189903 0.0 24 0.0 0.0 0.0 0.03840298446050665 0.0 25 0.0 0.0013715351593038088 0.0 0.04046028719946236 0.0 26 0.0 0.0013715351593038088 0.0 0.054175638792500445 0.0 27 0.0 0.0013715351593038088 0.0 0.10286513694778565 0.0 28 0.0 0.0013715351593038088 0.0 0.18104264102810275 0.0 29 0.0 0.0013715351593038088 0.0 0.2482478638339894 0.0 30 0.0 0.0013715351593038088 0.0 0.3421980222463003 0.0 31 0.0 0.0013715351593038088 0.0 0.44369162403478213 0.0 32 0.0 0.0013715351593038088 0.0 0.5396990851860487 0.0 33 0.0 0.0013715351593038088 0.0 0.6734237632181701 0.0 34 0.0 0.0013715351593038088 0.0 0.8338933768567157 0.0 35 0.0 0.0013715351593038088 0.0 1.068425889097667 0.0 36 0.0 0.0013715351593038088 0.0 1.412681214082923 0.0 37 0.0 0.0013715351593038088 0.0 1.9633525805434022 0.0 38 0.0 0.0013715351593038088 0.0 2.540083115030654 0.0 39 0.0 0.0013715351593038088 0.0 3.203220364554045 0.0 40 0.0 0.0013715351593038088 0.0 4.007625735485729 0.0 41 0.0 0.0013715351593038088 0.0 4.963585741520484 0.0 42 0.0 0.0013715351593038088 0.0 6.029954327879195 0.0 43 0.0 0.0013715351593038088 0.0 7.138154736596673 0.0 44 0.0 0.0013715351593038088 0.0 8.15994843027801 0.0 45 0.0 0.0013715351593038088 0.0 9.120023041790676 0.0 46 0.0 0.0013715351593038088 0.0 10.13358752451619 0.0 47 0.0 0.0013715351593038088 0.0 11.038114962077053 0.0 48 0.0 0.0013715351593038088 0.0 11.947442772695478 0.0 49 0.0 0.0013715351593038088 0.0 12.745676235410295 0.0 50 0.0 0.0013715351593038088 0.0 13.537737789908244 0.0 51 0.0 0.0013715351593038088 0.0 14.399061869951035 0.0 52 0.0 0.0013715351593038088 0.0 15.34747843260962 0.0 53 0.0 0.0013715351593038088 0.0 16.50368257190273 0.0 54 0.0 0.0013715351593038088 0.0 17.528905103482327 0.0 55 0.0 0.0013715351593038088 0.0 18.400515697219898 0.0 56 0.0 0.0013715351593038088 0.0 19.213836046687057 0.0 57 0.0 0.0013715351593038088 0.0 19.99355378475127 0.0 58 0.0 0.0013715351593038088 0.0 20.738983143832893 0.0 59 0.0 0.0013715351593038088 0.0 21.461782172786 0.0 60 0.0 0.0013715351593038088 0.0 22.17086585014607 0.0 61 0.0 0.0013715351593038088 0.0 22.838117705147372 0.0 62 0.0 0.0013715351593038088 0.0 23.546515614927788 0.0 63 0.0 0.0013715351593038088 0.0 24.32554758541235 0.0 64 0.0 0.0013715351593038088 0.0 25.140925237618465 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGTG 15 0.0022209994 70.0 67 CTCTGAA 15 0.0022209994 70.0 21 GTCTGCA 20 0.006939931 52.5 57 TATGGAC 20 0.006939931 52.5 67 CCGAGTT 20 0.006939931 52.5 60 CCAGTAT 20 0.006939931 52.5 31 CGAAGAC 20 0.006939931 52.5 21 AGACGTA 35 0.0012521665 40.0 5 GCCTAAA 35 0.0012521665 40.0 1 TCGCATA 40 0.0024125353 35.0 40 AGAAATC 50 1.7031828E-4 35.0 47 GAAATCG 55 2.9807424E-4 31.818184 48 GCCTTAA 45 0.004296252 31.111113 60 CGACGAT 45 0.004296252 31.111113 19 ATAGACG 45 0.004296252 31.111113 3 TTATCAA 45 0.004296252 31.111113 32 CATCAAT 45 0.004296252 31.111113 12 CTACTGA 60 4.96323E-4 29.166666 33 TCACATC 50 0.0071900613 27.999998 44 CTTCAGC 50 0.0071900613 27.999998 17 >>END_MODULE