FastQCFastQC Report
Wed 25 May 2016
SRR1780302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145822
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT58424.006254200326426No Hit
CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG17451.196664426492573RNA PCR Primer, Index 23 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG15281.04785286170811No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC15111.0361948128540275No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4240.2907654537724074No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT1980.13578198077107706RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT1860.1275527698152542No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGTAC150.002220999470.03
GCATGTA150.002220999470.02
TTAATAC252.3840954E-455.9999963
GATGTTA200.00693993152.533
CGTATGG200.00693993152.544
CCGTATG200.00693993152.543
ACAGCAT200.00693993152.565
ATACTAT352.0784333E-550.027
GGCACAC350.001252166540.012
CTAAAAT350.001252166540.063
TGCCTAC350.001252166540.045
ACGGTTT350.001252166540.07
CTTTATA951.8553692E-1036.8421062
CGTGCGA400.002412535335.012
TCTTCGT400.002412535335.020
GACAAAC400.002412535335.052
AGACGGT400.002412535335.05
TACGACA400.002412535335.049
GACGGTT400.002412535335.06
TCTTTAT1003.2196112E-1035.01