Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780302_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 145822 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT | 5842 | 4.006254200326426 | No Hit |
CACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTTCTG | 1745 | 1.196664426492573 | RNA PCR Primer, Index 23 (95% over 24bp) |
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG | 1528 | 1.04785286170811 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC | 1511 | 1.0361948128540275 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC | 424 | 0.2907654537724074 | No Hit |
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT | 198 | 0.13578198077107706 | RNA PCR Primer, Index 47 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT | 186 | 0.1275527698152542 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CATGTAC | 15 | 0.0022209994 | 70.0 | 3 |
GCATGTA | 15 | 0.0022209994 | 70.0 | 2 |
TTAATAC | 25 | 2.3840954E-4 | 55.999996 | 3 |
GATGTTA | 20 | 0.006939931 | 52.5 | 33 |
CGTATGG | 20 | 0.006939931 | 52.5 | 44 |
CCGTATG | 20 | 0.006939931 | 52.5 | 43 |
ACAGCAT | 20 | 0.006939931 | 52.5 | 65 |
ATACTAT | 35 | 2.0784333E-5 | 50.0 | 27 |
GGCACAC | 35 | 0.0012521665 | 40.0 | 12 |
CTAAAAT | 35 | 0.0012521665 | 40.0 | 63 |
TGCCTAC | 35 | 0.0012521665 | 40.0 | 45 |
ACGGTTT | 35 | 0.0012521665 | 40.0 | 7 |
CTTTATA | 95 | 1.8553692E-10 | 36.842106 | 2 |
CGTGCGA | 40 | 0.0024125353 | 35.0 | 12 |
TCTTCGT | 40 | 0.0024125353 | 35.0 | 20 |
GACAAAC | 40 | 0.0024125353 | 35.0 | 52 |
AGACGGT | 40 | 0.0024125353 | 35.0 | 5 |
TACGACA | 40 | 0.0024125353 | 35.0 | 49 |
GACGGTT | 40 | 0.0024125353 | 35.0 | 6 |
TCTTTAT | 100 | 3.2196112E-10 | 35.0 | 1 |