FastQCFastQC Report
Wed 25 May 2016
SRR1780301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681958
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAG300.005724129.35341339
CGCAAAG407.009924E-427.5127612
ACTCGCA755.4148368E-520.55947121
CTATAAC755.516798E-520.5097244
ATCGATA654.9283466E-420.30816723
AATCGAT654.9332285E-420.30518722
TACACAG654.967517E-420.2843425
TGTATAA1201.4477337E-820.1434762
CCGTATT550.00446392420.01368914
CCCGAAA550.00449437619.99019410
TTACTCG808.898935E-519.27308719
TAATCGA708.049201E-418.88114721
ATTCGCA600.00737967718.34588239
TTAAACG600.00737967718.34588214
CAAAGGC600.00737967718.34588214
TCCGTAT600.00738593418.34318713
TTGGTAA600.007445588718.31762533
ACCGACA600.00745819818.3122527
GCTCTAG600.00745819818.3122521
TGCGTGT1456.359187E-918.19129833
Produced by FastQC (version 0.11.5)