FastQCFastQC Report
Wed 25 May 2016
SRR1780301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681958
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT204733.002091037864502No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC55230.8098739218544251No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG52660.7721883165825462No Hit
CACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTG33980.498271154528578Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC12040.1765504620519152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA1550.035.4842262
CGGTAAG250.00235280735.1977729
ATGCCGT29700.035.10888744
TCTTCTG3950.033.97253444
GAATCAT502.3164357E-630.8727471
CGTCTTC4450.030.64974642
CCGTCTT4450.030.64974641
TCTTTAT1800.030.6277261
TATGCCG34850.030.04687543
GTATCAC300.00567743729.4026171
GTCTTCT4650.029.33147643
CGCAAAG300.005745057529.33147612
AACGCTA407.028555E-427.5002775
GCTAAAC407.028555E-427.5002773
TACGGTC655.9281047E-727.07520710
CCTTATA508.203463E-526.4623551
TTTATAC2700.026.0743353
TTAATAC701.1309603E-625.143113
GCCGTCT5400.024.85027740
GGCTTAT450.0013785124.502181
Produced by FastQC (version 0.11.5)