FastQCFastQC Report
Wed 25 May 2016
SRR1780300_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780300_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85385
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTATA150.002217834870.01
GATACGC200.00693011752.53
CCAGGCT200.00693011752.568
GCCATGG200.00693011752.532
CTGAAAA200.00693011752.51
TGTACGC200.00693011752.52
GTACGCG200.00693011752.53
TTAGAGG305.8486965E-446.6666644
GATGAGT305.8486965E-446.66666437
TATACCC753.4768163E-837.3333323
GCTTCCG450.00428636531.11111318
TCGAGTG450.00428636531.11111343
TACCCCT853.9028346E-628.8235285
TTATACC853.9028346E-628.8235282
CCGTATC700.001217272525.055
ATACCCC700.001217272525.04
TAGATCT1001.3791398E-524.50000239
AGATCTC1052.0121071E-523.33333240
ATACACC1355.9177637E-620.740744
TTAAAAA950.00715740218.42105163
Produced by FastQC (version 0.11.5)