FastQCFastQC Report
Wed 25 May 2016
SRR1780300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85385
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT909810.655267318615682No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC25943.038004333313814No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG22842.6749429056625873No Hit
CACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTG20812.437196228845816Illumina PCR Primer Index 9 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC5700.6675645605199977No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT3020.35369210048603383No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT2850.33378228025999884Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1630.19090004099080635No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1380.16162089359957837No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1320.15459389822568365No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1040.12180125314750834No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT860.10072026702582422No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAAC150.002217834870.028
CAATACC150.002217834870.04
TTATATA308.314089E-658.3333322
TATTCTA200.00693011752.534
CCAAACG200.00693011752.59
CTTATAT352.0711179E-550.01
GTGTGGT305.8486965E-446.6666641
TCTTTAT551.2935743E-744.5454561
AGCCAGA459.130496E-538.888891
TCTCGTA19050.037.48031238
CCGTCTT20250.037.16049247
TCGTATG20450.037.13936640
CTCGTAT20000.037.139
ATCTCGT19250.037.09090837
CGTATGC20400.037.05882341
GTCTTCT20400.037.05882349
GCCGTCT20350.036.97788646
TATGCCG20450.036.96821643
TGAAAAA20100.036.91542459
CGTCTTC20400.036.88725748
Produced by FastQC (version 0.11.5)