FastQCFastQC Report
Wed 25 May 2016
SRR1780299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363125
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAT250.00236719535.1496541
ATTCCAT300.005694984529.38038618
GGATAGC300.005780075729.291388
TCTACAG300.005780075729.291383
TACGTCA300.005780075729.291385
GATAGCG300.005780075729.291389
ATGCAGT450.001404637524.4195731
TCAGATG450.001408023624.4094834
TATGCAG651.8095994E-523.69104830
GTATAGA500.002598498721.9685341
GATGATT550.0044363120.03208415
TTATCCA550.004514915419.9713952
CATGTAC550.004514915419.9713953
GACGAAA1152.0128209E-719.12941623
CGAAGTA1051.7865514E-618.85613825
AAGTATG1102.7869028E-617.9990427
CTGCCGA4600.017.72403518
TTTTCGT750.001273551617.63066920
ACGAAGT1154.2193897E-617.23310924
ACGCTGC5350.016.88685615
Produced by FastQC (version 0.11.5)