FastQCFastQC Report
Wed 25 May 2016
SRR1780299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363125
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT203325.599173838209983No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC62341.716764199655766No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG51111.4075043029259897No Hit
CACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTG37741.0393115318416524RNA PCR Primer, Index 36 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC11400.31394148020654045No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT4880.13438898450946643RNA PCR Primer, Index 15 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT1300.039.0123331
CTTTATA1350.037.4794852
TATGGCG250.00235124635.1981285
TCTTCTG4850.034.47239344
ATGCCGT27800.033.631344
GTCTTCT5050.033.1071543
TTTATAC1750.032.6839753
GAGTATA353.1705748E-431.500641
TTTGCAA353.214467E-431.42689740
CGTCTTC5500.030.39838242
CCGTCTT5600.029.85555341
TGGAATA300.00574128129.33177638
GGCAAAA300.00574128129.33177638
TCCTATA300.00574128129.3317762
ATCCATA300.00574128129.33177618
GGACCAA300.00574128129.33177613
TAGTTGC454.063273E-529.33177421
TATGCCG33350.027.96852743
ATAATGC407.024781E-427.49853738
GGATAAT407.024781E-427.49853736