FastQCFastQC Report
Wed 25 May 2016
SRR1780298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176165
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTCG150.002220018570.0139127
TAGTTAG200.00694465652.495539
ATACGTA200.00694465652.4955370
AGAGCGG200.00694465652.495535
TATGGCA305.868245E-446.6626948
CTTCCGA404.5812034E-543.74627718
GGATAAT400.002414903634.997026
TGTAAGT400.002414903634.997023
AGTTGAA450.004300451431.10846320
ACGCTTC604.9698586E-429.1641815
AGGTAGT500.00719705727.9976166
TGGAATT500.00719705727.99761623
CCCTATA657.936411E-426.9207842
GGGTTAG750.001827809823.3313471
CTAGGGA750.001827809823.3313474
TGACTCT750.001827809823.33134710
ACCCTAT900.00526208619.442791
AATATCT900.00526208619.4427938
GGGACGA900.00526208619.442795
CTCCTAG900.00526208619.442791
Produced by FastQC (version 0.11.5)