##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780298_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176165 Sequences flagged as poor quality 0 Sequence length 76 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.670734822467573 34.0 32.0 34.0 21.0 34.0 2 30.715045553884142 34.0 32.0 34.0 21.0 34.0 3 30.458763091419975 34.0 32.0 34.0 11.0 34.0 4 30.279703686884456 34.0 31.0 34.0 11.0 34.0 5 30.338165923991713 34.0 31.0 34.0 11.0 34.0 6 33.396741691028296 37.0 34.0 38.0 21.0 38.0 7 33.80274742428973 38.0 34.0 38.0 21.0 38.0 8 33.76915959469815 38.0 34.0 38.0 21.0 38.0 9 34.051343910538414 38.0 35.0 38.0 21.0 38.0 10-11 33.781205120199814 38.0 34.0 38.0 21.0 38.0 12-13 33.87201487242074 38.0 34.0 38.0 21.0 38.0 14-15 33.798180682882524 38.0 34.0 38.0 21.0 38.0 16-17 33.86621916952856 38.0 34.0 38.0 21.0 38.0 18-19 33.88125337041978 38.0 34.0 38.0 21.0 38.0 20-21 33.89721000198678 38.0 34.0 38.0 21.0 38.0 22-23 33.91010132546192 38.0 34.0 38.0 21.0 38.0 24-25 33.899057701586585 38.0 34.0 38.0 21.0 38.0 26-27 33.857525615190305 38.0 34.0 38.0 21.0 38.0 28-29 33.93664462293872 38.0 34.5 38.0 21.0 38.0 30-31 33.95493997104987 38.0 34.0 38.0 21.0 38.0 32-33 34.01921777878694 38.0 35.0 38.0 21.0 38.0 34-35 33.94952459342094 38.0 34.5 38.0 21.0 38.0 36-37 33.86047455510459 38.0 34.0 38.0 21.0 38.0 38-39 33.84651321204552 38.0 34.0 38.0 21.0 38.0 40-41 33.95941588851361 38.0 34.5 38.0 21.0 38.0 42-43 34.0219339823461 38.0 35.0 38.0 21.0 38.0 44-45 34.13108733289813 38.0 35.0 38.0 22.0 38.0 46-47 34.104720006811796 38.0 35.0 38.0 21.5 38.0 48-49 34.05608946158431 38.0 35.0 38.0 21.0 38.0 50-51 33.9973065024267 38.0 35.0 38.0 21.0 38.0 52-53 34.056747935174414 38.0 35.0 38.0 21.0 38.0 54-55 34.03844123407033 38.0 35.0 38.0 21.0 38.0 56-57 34.01472199358556 38.0 35.0 38.0 21.0 38.0 58-59 34.07144438452587 38.0 35.0 38.0 21.5 38.0 60-61 34.09457043112991 38.0 35.0 38.0 22.0 38.0 62-63 33.96801861890841 38.0 35.0 38.0 21.5 38.0 64-65 33.946107342548174 38.0 35.0 38.0 21.0 38.0 66-67 33.79707944256805 38.0 34.0 38.0 20.0 38.0 68-69 33.74818777850311 38.0 34.0 38.0 20.0 38.0 70-71 33.739096869412194 38.0 34.0 38.0 20.0 38.0 72-73 33.744112054040244 38.0 34.0 38.0 20.0 38.0 74-75 33.722036159282496 38.0 34.0 38.0 20.0 38.0 76 31.772571169074446 36.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.44267221214286323 1101 2 -0.11184219586728616 1101 3 0.18088270371529447 1101 4 0.12678131605668597 1101 5 -0.5056370655347635 1101 6 0.09141065725461317 1101 7 -0.43047892574457336 1101 8 -0.8333181628278581 1101 9 -0.2996462636689685 1101 10-11 -0.42757380672421874 1101 12-13 -0.29120976333866366 1101 14-15 -0.036280275487186486 1101 16-17 -0.41025989869991264 1101 18-19 -0.2988257375808985 1101 20-21 -0.12230504503892803 1101 22-23 -0.273610905197998 1101 24-25 -0.3335356086331487 1101 26-27 -0.6904607026251313 1101 28-29 -0.7451275711449483 1101 30-31 -0.8007479555514152 1101 32-33 -0.3326703659826933 1101 34-35 -0.5062913961491375 1101 36-37 -0.31128053841846537 1101 38-39 -0.2183034408460358 1101 40-41 -0.42422172273607117 1101 42-43 -0.3001712479016234 1101 44-45 -0.4279949443205169 1101 46-47 -0.38855244983077597 1101 48-49 -0.38790094408351905 1101 50-51 0.015023937956662792 1101 52-53 -0.2623241900169049 1101 54-55 -0.2651885872584572 1101 56-57 -0.5500705529906895 1101 58-59 -0.28462751569490763 1101 60-61 -0.2287975638704367 1101 62-63 -0.5176710576538426 1101 64-65 -0.25709138605998305 1101 66-67 -0.5458981002279089 1101 68-69 -0.47267644425967603 1101 70-71 -0.19377934708266054 1101 72-73 -0.2249647287518357 1101 74-75 -0.482075525593487 1101 76 -1.429859631871075 1102 1 0.4252427722684935 1102 2 0.3437186734180244 1102 3 0.7412691549306558 1102 4 0.6441541467520615 1102 5 0.056257667434987724 1102 6 0.30135611999362055 1102 7 -0.18220563244814514 1102 8 -0.01659278966607758 1102 9 0.8259043971239848 1102 10-11 0.6604169596484653 1102 12-13 0.4069453505642002 1102 14-15 0.17090153684209497 1102 16-17 0.11913090262076054 1102 18-19 0.049288450384125326 1102 20-21 0.14733480670801669 1102 22-23 0.20616064529724554 1102 24-25 0.13781853408341505 1102 26-27 0.23722224551389104 1102 28-29 -0.1137231217137753 1102 30-31 0.23018101725352835 1102 32-33 0.2351655256049554 1102 34-35 3.6481677610566976E-4 1102 36-37 0.22351800480208794 1102 38-39 0.04260631056338582 1102 40-41 -0.04534640429548631 1102 42-43 0.012229311266246157 1102 44-45 0.14923551929217638 1102 46-47 -0.16117207772663278 1102 48-49 0.05978743019107213 1102 50-51 0.17115869005584017 1102 52-53 0.2614772318145526 1102 54-55 0.20533783986117982 1102 56-57 0.30587934262517535 1102 58-59 -0.12345059608448139 1102 60-61 -0.23651371260173448 1102 62-63 -0.10576528847613531 1102 64-65 0.08991918110913844 1102 66-67 -0.11752768830144333 1102 68-69 -0.08786916404116596 1102 70-71 -0.12950263256176697 1102 72-73 0.138032245487814 1102 74-75 -0.45115804883850075 1102 76 0.34332330247268317 1103 1 0.21524418691999614 1103 2 0.4867656612820319 1103 3 0.5959158564349032 1103 4 0.6036693928371193 1103 5 -0.02959024466800031 1103 6 -0.10311846557779347 1103 7 0.3943634518436454 1103 8 0.1946061060483828 1103 9 0.4604013170418497 1103 10-11 0.4437502215347493 1103 12-13 0.5649083381366964 1103 14-15 0.4361979920683723 1103 16-17 0.610814466513709 1103 18-19 0.4781057588595985 1103 20-21 0.44645858014085604 1103 22-23 0.5172103595805169 1103 24-25 0.549930094358281 1103 26-27 0.22102877263018428 1103 28-29 0.4170991337551371 1103 30-31 0.3378059888533258 1103 32-33 0.4645191156046522 1103 34-35 -0.013665954037492156 1103 36-37 0.26285174681252244 1103 38-39 -0.07590504737345327 1103 40-41 0.47034765373951615 1103 42-43 0.08608054994368075 1103 44-45 0.5406524428109805 1103 46-47 0.26949285134789136 1103 48-49 0.40471590455373274 1103 50-51 -0.3106416040201978 1103 52-53 0.43987121654489414 1103 54-55 0.3510833826372419 1103 56-57 0.687095771444632 1103 58-59 0.3085325309812319 1103 60-61 0.276050964836287 1103 62-63 0.3948541574091564 1103 64-65 0.4674832369578681 1103 66-67 0.478475706862163 1103 68-69 0.7577259112568839 1103 70-71 0.7803497721314514 1103 72-73 0.5355193100002111 1103 74-75 0.4186811524406835 1103 76 0.3762761088411892 1104 1 0.2503517086513405 1104 2 0.07528418134317505 1104 3 0.18522990819027996 1104 4 0.6066224398068094 1104 5 0.002172711877644673 1104 6 0.059036717248716286 1104 7 0.29219802290212016 1104 8 -0.2047383091941697 1104 9 0.3715433423572492 1104 10-11 0.42202096054619886 1104 12-13 0.01850509936473088 1104 14-15 0.1972177720684556 1104 16-17 0.19400375724097074 1104 18-19 0.11639707340283678 1104 20-21 -0.5138148161963585 1104 22-23 0.20457265137820713 1104 24-25 0.09136230131314704 1104 26-27 0.14102369122355185 1104 28-29 0.1525411120050535 1104 30-31 0.1687679990911164 1104 32-33 0.23915456632958865 1104 34-35 0.2545073635795774 1104 36-37 0.07980032047833419 1104 38-39 0.4591858140082721 1104 40-41 0.05328495667881583 1104 42-43 0.31154922640384797 1104 44-45 0.338556154320365 1104 46-47 0.40558083465421646 1104 48-49 0.005710943934637669 1104 50-51 0.15824935815664531 1104 52-53 0.010790159778913733 1104 54-55 0.3546007989502229 1104 56-57 0.3211262015732075 1104 58-59 0.4296074567385446 1104 60-61 0.3658356714683606 1104 62-63 0.24900737431441655 1104 64-65 0.39296982329388186 1104 66-67 0.4079670247374665 1104 68-69 0.1657482861064139 1104 70-71 0.43119049502401907 1104 72-73 0.6887591232376877 1104 74-75 0.3537516048941285 1104 76 0.6644876619606279 1105 1 0.691375637146205 1105 2 0.7478422710214332 1105 3 0.5930903610341574 1105 4 0.7833032887654028 1105 5 1.1961196767022635 1105 6 1.2165673493501714 1105 7 0.8778194876360388 1105 8 0.4549773853258614 1105 9 0.5230588686686986 1105 10-11 0.9662561062445221 1105 12-13 -0.2123220086020865 1105 14-15 0.7197053625630261 1105 16-17 0.7287215131406342 1105 18-19 0.8613673070808261 1105 20-21 0.7157424819121161 1105 22-23 0.9792185063560268 1105 24-25 0.7916748064665953 1105 26-27 0.7397575918485586 1105 28-29 0.3946955010945814 1105 30-31 0.793030025179938 1105 32-33 0.651333609519611 1105 34-35 0.7671766028480391 1105 36-37 0.8247684927766912 1105 38-39 1.121418453068948 1105 40-41 0.6539318176099016 1105 42-43 0.9027405389254923 1105 44-45 0.6119033401248757 1105 46-47 0.9117546666765151 1105 48-49 1.128954648481212 1105 50-51 0.7226633491525405 1105 52-53 0.5841175563388887 1105 54-55 0.9968050734270477 1105 56-57 0.6033847315621585 1105 58-59 0.6420181990001268 1105 60-61 0.8078325711533836 1105 62-63 0.8755410407666986 1105 64-65 0.47301319472150993 1105 66-67 0.7317602930961158 1105 68-69 0.8729952723693799 1105 70-71 0.5606080315928921 1105 72-73 0.9072117692245953 1105 74-75 0.8295716217149902 1105 76 0.7019676665781205 1106 1 0.272701338158317 1106 2 0.33588773310870934 1106 3 0.5280911906342922 1106 4 0.8694406705473838 1106 5 0.508837446737104 1106 6 0.7253942947624807 1106 7 -0.21519527924632342 1106 8 0.29269100736004106 1106 9 0.43406766243010964 1106 10-11 0.22567621575174002 1106 12-13 0.5395238516932466 1106 14-15 0.6853640650684127 1106 16-17 0.7108270382775217 1106 18-19 0.9247155732165524 1106 20-21 0.6541985960650933 1106 22-23 0.8512609818828238 1106 24-25 0.7916913984692542 1106 26-27 0.36190376739195074 1106 28-29 0.8421735166910622 1106 30-31 0.4053205511464171 1106 32-33 0.45828980663260666 1106 34-35 0.5130783781923185 1106 36-37 0.6799493456056283 1106 38-39 0.5907273961583783 1106 40-41 0.6891110112385732 1106 42-43 0.6046071392241501 1106 44-45 0.4221489017641673 1106 46-47 0.71703507152138 1106 48-49 0.41674708252799775 1106 50-51 0.33799435960549573 1106 52-53 0.3990171747228217 1106 54-55 0.452342654313064 1106 56-57 0.3846233245603301 1106 58-59 0.300052876285676 1106 60-61 0.5962181692950779 1106 62-63 0.40974960224006907 1106 64-65 0.5126514890636358 1106 66-67 0.37208715554841376 1106 68-69 0.8032922692168967 1106 70-71 0.8455176051784221 1106 72-73 0.6151593384962126 1106 74-75 0.6727523086238989 1106 76 0.8508560024002563 1107 1 -0.15480475862001697 1107 2 -0.25516176845292193 1107 3 -0.41921980923735447 1107 4 -0.5677347027465878 1107 5 -0.3778052606603204 1107 6 -0.9994933961629471 1107 7 -0.22140329704463824 1107 8 -0.3697656734254835 1107 9 -0.21812076010871806 1107 10-11 -0.37521369996297693 1107 12-13 0.020452701220484926 1107 14-15 -0.11929729633148867 1107 16-17 -0.3504445143686894 1107 18-19 -0.14271051671627788 1107 20-21 0.10898403225215247 1107 22-23 0.05889585973334022 1107 24-25 0.24096406351011268 1107 26-27 0.15828420635999407 1107 28-29 0.024112078279216576 1107 30-31 -0.21972116365270722 1107 32-33 -0.2112156055951857 1107 34-35 0.027530951674542337 1107 36-37 0.161329570651624 1107 38-39 -0.46933549692338516 1107 40-41 0.17549059451842908 1107 42-43 -0.07029980468548302 1107 44-45 -0.4323263474550174 1107 46-47 0.09342235599491744 1107 48-49 0.01236595900941495 1107 50-51 0.24958465610145453 1107 52-53 -0.07677112801965791 1107 54-55 0.09869314419454867 1107 56-57 0.2654430738436915 1107 58-59 -0.13622616363218043 1107 60-61 -0.019070839390153083 1107 62-63 0.12861538754790303 1107 64-65 -0.09214250400528812 1107 66-67 -0.01048033685735561 1107 68-69 0.38891029001012356 1107 70-71 -0.3418182096506541 1107 72-73 -0.0027309622781928056 1107 74-75 0.14967439821445794 1107 76 -0.19076894946728373 1108 1 0.08170493959889313 1108 2 0.3699813705636892 1108 3 0.3498198821108822 1108 4 0.8119976933565063 1108 5 0.42270299295559255 1108 6 1.2094279082078572 1108 7 0.9059331721342261 1108 8 0.6353080541787648 1108 9 0.38892960720801995 1108 10-11 0.6572788568081549 1108 12-13 0.1558203703006953 1108 14-15 0.7152560218511326 1108 16-17 0.41959054367968207 1108 18-19 0.5332689945851001 1108 20-21 0.3832802287181565 1108 22-23 0.3529716661565345 1108 24-25 0.37269379942963354 1108 26-27 0.5229781971638587 1108 28-29 0.5062791589479616 1108 30-31 0.6148343566686307 1108 32-33 0.5489560731392089 1108 34-35 0.6609475258513839 1108 36-37 0.12058560010743236 1108 38-39 0.6144668919566953 1108 40-41 0.7178024878292106 1108 42-43 0.4685327094522407 1108 44-45 0.2971188897593606 1108 46-47 0.48990421918044547 1108 48-49 0.24585872718586899 1108 50-51 0.39828847725255656 1108 52-53 0.5356018085763665 1108 54-55 0.36908935299253187 1108 56-57 0.41316474112696255 1108 58-59 0.5422197430323692 1108 60-61 0.7027307743370983 1108 62-63 0.5973846562616743 1108 64-65 0.735370576698692 1108 66-67 0.7792000006864654 1108 68-69 0.713886506911976 1108 70-71 0.7483087216250013 1108 72-73 0.5777043565034106 1108 74-75 0.7471520685278676 1108 76 0.9701237094830901 1109 1 0.1301183348077224 1109 2 0.15964649034294354 1109 3 0.1767357230741311 1109 4 0.00160637264911756 1109 5 -0.07005381125863153 1109 6 0.5134539962550164 1109 7 0.46281264033942193 1109 8 0.5754811383377998 1109 9 0.28645876812611704 1109 10-11 0.6380734134367785 1109 12-13 0.08437612095916336 1109 14-15 0.35507164959476256 1109 16-17 0.4103092192096014 1109 18-19 0.35756662408466156 1109 20-21 0.09410112424053807 1109 22-23 0.21527255666198641 1109 24-25 -0.04447978642261319 1109 26-27 0.1087207031214632 1109 28-29 -0.034326635608664446 1109 30-31 0.30264007597127573 1109 32-33 0.20825934668742718 1109 34-35 0.6267853535197645 1109 36-37 0.19938279062542819 1109 38-39 0.696164496371594 1109 40-41 0.41562639351088393 1109 42-43 0.5456366853709724 1109 44-45 0.3624017193090978 1109 46-47 0.44161624727526316 1109 48-49 0.476418115120147 1109 50-51 0.5177384960660518 1109 52-53 0.20236776869175088 1109 54-55 0.3218674791683753 1109 56-57 0.28361814636964766 1109 58-59 0.45763740263353014 1109 60-61 0.5483503654571251 1109 62-63 0.45393175271219377 1109 64-65 0.4242110382564448 1109 66-67 0.7157514869526125 1109 68-69 0.3196340298017333 1109 70-71 0.3089352110267285 1109 72-73 -0.20998534624335718 1109 74-75 0.521092241612024 1109 76 -0.11256160344805366 1110 1 -0.5136630463051475 1110 2 -0.10152174681229198 1110 3 -0.06477418132094215 1110 4 -0.35866169943286863 1110 5 -0.274572691511743 1110 6 0.12049798139842949 1110 7 -0.22065940037865062 1110 8 0.061914180500259874 1110 9 0.06179149389817695 1110 10-11 -0.2932836262495826 1110 12-13 0.25076479968534926 1110 14-15 0.001266797128984365 1110 16-17 -0.1421724679765859 1110 18-19 -0.32831273488088186 1110 20-21 -0.2806022597611104 1110 22-23 -0.09860735254771669 1110 24-25 -0.3363821696676794 1110 26-27 -0.10944251305571129 1110 28-29 -0.2293538039145986 1110 30-31 -0.04677786886674795 1110 32-33 -0.009587908969763248 1110 34-35 0.13515293382101845 1110 36-37 0.16332245291307146 1110 38-39 -0.3149177651319448 1110 40-41 -0.2522690085112629 1110 42-43 0.14703636898057226 1110 44-45 0.06545041591822098 1110 46-47 -0.15513000418632572 1110 48-49 -0.14329653169188106 1110 50-51 0.003864175273704973 1110 52-53 0.25125137485448334 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-0.4568783616719756 2116 9 -0.8277653276466594 2116 10-11 -0.38410191568431884 2116 12-13 -0.0762550286662318 2116 14-15 -0.178603356762693 2116 16-17 0.15185868029212912 2116 18-19 -0.2954257860794627 2116 20-21 -0.11655594428315652 2116 22-23 -0.22279350229617023 2116 24-25 -0.6435828947342657 2116 26-27 -0.15623890252005168 2116 28-29 -0.6399412558234445 2116 30-31 -0.39360755220501176 2116 32-33 -0.37403560059451024 2116 34-35 -0.5693546867767907 2116 36-37 -0.49729274389071776 2116 38-39 -0.24344691576823152 2116 40-41 -0.8457045663686173 2116 42-43 -0.33783341497917974 2116 44-45 -0.16660788535435245 2116 46-47 -0.46107296068232984 2116 48-49 -0.16918251867027578 2116 50-51 -0.1297644870524266 2116 52-53 -0.28303206699221306 2116 54-55 -0.06288389502227432 2116 56-57 -0.10396963555949412 2116 58-59 -0.4572116809133533 2116 60-61 -0.581730716681335 2116 62-63 -0.11106110736487551 2116 64-65 -0.8817175429528419 2116 66-67 -0.6093301820288204 2116 68-69 -0.5623836341719795 2116 70-71 -0.477329239981664 2116 72-73 -0.4952179316779137 2116 74-75 -0.2428159370229821 2116 76 -0.5761333254409742 2117 1 -0.17555074045090535 2117 2 -0.6057033477680882 2117 3 0.08038710900163792 2117 4 -0.1722070996718159 2117 5 -0.10028953738988022 2117 6 0.12109535296353613 2117 7 -0.2455996632221371 2117 8 -0.29180445749256734 2117 9 -0.4518733806537938 2117 10-11 0.13518112882807998 2117 12-13 -0.482844817996849 2117 14-15 -0.18727860310995936 2117 16-17 -0.3084284416922358 2117 18-19 -0.5860412295045307 2117 20-21 -0.24767215223423023 2117 22-23 -0.1642435532645692 2117 24-25 -0.2042883823319599 2117 26-27 0.1310742133481142 2117 28-29 -0.2073257274510354 2117 30-31 -0.17879698475683625 2117 32-33 -0.38048097945960535 2117 34-35 0.020084236091037155 2117 36-37 -0.4225497095719959 2117 38-39 -0.07918001124902929 2117 40-41 -0.25779469548856326 2117 42-43 -0.24439134906005222 2117 44-45 -0.4468703726122527 2117 46-47 -0.3009260017968387 2117 48-49 -0.28181206335256803 2117 50-51 -0.07162865626751369 2117 52-53 -0.11374265142985962 2117 54-55 -0.13999483623872067 2117 56-57 -0.33016314558328475 2117 58-59 -0.21768143966293252 2117 60-61 -0.1637686031916985 2117 62-63 -0.2474546683570651 2117 64-65 -0.26614669687740644 2117 66-67 -0.20850619290207817 2117 68-69 -0.6076393256432624 2117 70-71 -0.1045060153809132 2117 72-73 -1.9311464060238208 2117 74-75 -0.48580778207904984 2117 76 -0.5094709061409404 2118 1 0.011453604440681886 2118 2 -0.04880846883448342 2118 3 -0.01742040808264278 2118 4 -0.5435898719657501 2118 5 -0.16005288844024435 2118 6 0.0938290490742304 2118 7 -0.1023553368912502 2118 8 0.38370490722522277 2118 9 -0.2150978807803412 2118 10-11 -0.7892789234793511 2118 12-13 0.12188868828847887 2118 14-15 0.2711364605869875 2118 16-17 0.21423459720086413 2118 18-19 0.24366169380601832 2118 20-21 0.0724683185326711 2118 22-23 0.07539514579896434 2118 24-25 0.14125441274102712 2118 26-27 0.20745255638362892 2118 28-29 0.48524682802015207 2118 30-31 0.3917600472181917 2118 32-33 0.07462795920570642 2118 34-35 0.07932820386290729 2118 36-37 0.12379667952780693 2118 38-39 -0.008475337326821375 2118 40-41 -2.7813849876423546E-4 2118 42-43 0.4830088871670668 2118 44-45 0.524861467428245 2118 46-47 -0.03855044286660814 2118 48-49 -0.03793656769746434 2118 50-51 0.04743139182157563 2118 52-53 0.11024863769525695 2118 54-55 0.15547361056800213 2118 56-57 -0.030708366202532034 2118 58-59 -0.1402590571437301 2118 60-61 -0.16269819907459748 2118 62-63 -0.11945173660981823 2118 64-65 0.0013805114242870786 2118 66-67 0.09370632713504534 2118 68-69 0.014581841993617672 2118 70-71 0.07325225256813184 2118 72-73 0.3023124229646186 2118 74-75 0.21385685978377467 2118 76 -0.19708691052801441 2119 1 0.19897982157965544 2119 2 -0.4969556140203544 2119 3 -0.37022477911024865 2119 4 -0.22478415224886916 2119 5 0.31220075010040915 2119 6 -0.3434701741019879 2119 7 -0.17855097117344343 2119 8 -0.05525628344439326 2119 9 -0.02957345835387315 2119 10-11 0.14434326299021194 2119 12-13 0.11924175283972716 2119 14-15 0.25220436762300835 2119 16-17 0.28419652593272815 2119 18-19 0.15164816443486018 2119 20-21 0.08129055172847188 2119 22-23 -0.3512579277789456 2119 24-25 0.22292445113087922 2119 26-27 -0.07708105730715431 2119 28-29 0.1920688594435518 2119 30-31 0.013203144743300754 2119 32-33 -0.121699251927879 2119 34-35 -0.23749961524281105 2119 36-37 0.019329013556728114 2119 38-39 -0.37908612365514927 2119 40-41 0.19936504292117974 2119 42-43 -0.020777000445697524 2119 44-45 0.10931649607461935 2119 46-47 -0.21702610539693978 2119 48-49 0.5388059760154675 2119 50-51 0.31738163899277794 2119 52-53 0.06424718280997155 2119 54-55 -0.09008872383260069 2119 56-57 0.08279657987644384 2119 58-59 0.3675782080967238 2119 60-61 -0.2353336122567029 2119 62-63 -0.03819055284294848 2119 64-65 0.14100171027099861 2119 66-67 -0.20601331540920143 2119 68-69 -0.36154051704446033 2119 70-71 -0.17930043392532724 2119 72-73 0.2555118431344283 2119 74-75 -0.1681115893828533 2119 76 -0.0017818359518670945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 25.0 13 304.0 14 1216.0 15 2055.0 16 2529.0 17 3054.0 18 3126.0 19 2736.0 20 2186.0 21 1677.0 22 1303.0 23 1095.0 24 1184.0 25 1195.0 26 1457.0 27 1663.0 28 2060.0 29 2533.0 30 3181.0 31 4026.0 32 4959.0 33 6624.0 34 9073.0 35 13266.0 36 24340.0 37 79297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.582266625039026 15.05974512530866 8.776431186671587 30.58155706298073 2 25.763346862316578 16.898929980416085 27.383418953821703 29.95430420344563 3 22.040132830017313 18.457695910084297 24.21650157522777 35.28566968467062 4 18.660346833934096 15.098912950926687 25.30071240030653 40.94002781483269 5 19.47322112791985 28.015213010529898 28.830357903102204 23.681207958448045 6 37.22305792864644 26.608577186160705 14.891153180257144 21.277211704935713 7 35.018874350750714 24.026906593250647 15.76590128572645 25.18831777027219 8 29.316266000624413 27.721738143218005 17.754945647546336 25.207050208611243 9 25.96088893934664 15.151136718417394 16.68095251610706 42.207021826128916 10-11 30.62554991059518 19.505009508131582 21.12309482587347 28.746345755399766 12-13 28.773309113615074 17.086538188629977 21.663781114296256 32.47637158345869 14-15 27.411233786506966 18.023443930406152 19.146254931456305 35.419067351630574 16-17 29.57028921749496 20.20634064655295 19.535946413873358 30.687423722078734 18-19 28.72505889365084 19.10311355831181 20.304544035421337 31.867283512616012 20-21 28.109726676695146 19.62705418215877 20.938041041069454 31.325178100076634 22-23 29.697726563165215 19.55240825362586 19.666222007776803 31.083643175432123 24-25 29.079272273153013 20.196122952913463 19.05259273976102 31.672012034172507 26-27 27.985127579258084 20.397638577469984 20.472284506002893 31.14494933726904 28-29 28.80452984418017 19.90860840689127 21.348451735588796 29.938410013339766 30-31 28.443017747616572 19.456530685807866 21.142171095765605 30.958280470809953 32-33 27.33800698209065 20.43198138109159 21.813356796185392 30.41665484063236 34-35 27.784747253994833 20.047682570317598 21.60616467516249 30.561405500525073 36-37 27.820793006556354 19.059688360343998 21.93682059432918 31.182698038770475 38-39 26.313967019555527 19.088922317145858 24.496920500666988 30.100190162631623 40-41 26.058808503391706 18.238299321658673 25.292765305253596 30.410126869696025 42-43 24.213947151817898 18.049839638974824 26.020492152243634 31.71572105696364 44-45 22.808446626741976 18.037635171572106 28.382482331904747 30.771435869781172 46-47 22.37504612153379 19.485141770499247 28.72704566741407 29.41276644055289 48-49 21.5377628927426 20.639741151761132 28.020038032526323 29.802457922969943 50-51 20.622995487185307 22.117617006783412 27.01756875656345 30.241818749467832 52-53 19.745692958306133 22.447989101126783 27.23753299463571 30.56878494593137 54-55 19.620865724933232 21.5713154466422 27.52455801253942 31.283260815885157 56-57 20.01163681775608 22.251582323390004 27.015298157976897 30.72148270087702 58-59 20.624130786478588 22.018845968268387 26.27139329605767 31.085629949195358 60-61 20.910793858030825 21.142111089035847 26.315953793318762 31.631141259614566 62-63 21.029148809354865 21.255641018363466 25.60156671302472 32.11364345925695 64-65 21.638520705020863 21.243436550960748 24.316124088212753 32.801918655805636 66-67 21.8304482458157 22.391287688495694 22.99782305742644 32.78044100826216 68-69 21.968325149717593 23.54837794113473 22.399738881162545 32.08355802798513 70-71 22.379587318706896 24.937416626458152 21.578066017653903 31.10493003718105 72-73 22.809014276388613 25.241676837056172 21.32375897596004 30.62554991059518 74-75 22.209860074362105 26.451905883688585 22.34183861720546 28.99639542474385 76 23.12264070615616 25.166179434053305 22.847898277183322 28.863281582607215 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 1.0 14 0.5 15 1.0 16 2.5 17 3.0 18 4.0 19 15.5 20 26.5 21 27.0 22 34.0 23 54.5 24 92.5 25 117.0 26 162.0 27 241.5 28 338.5 29 401.0 30 515.0 31 711.5 32 994.5 33 1195.0 34 1362.0 35 1753.0 36 2190.5 37 2404.0 38 2715.0 39 3434.0 40 4231.0 41 4891.5 42 5163.0 43 5288.0 44 5728.5 45 6068.0 46 6092.0 47 6111.0 48 5931.0 49 5560.5 50 5389.0 51 5265.0 52 4690.0 53 4144.5 54 4050.0 55 4170.0 56 4520.0 57 5125.0 58 5500.0 59 6069.0 60 7037.0 61 7878.5 62 8321.0 63 8658.5 64 8964.5 65 8744.0 66 8280.0 67 8005.0 68 7528.5 69 6646.0 70 5753.0 71 5266.0 72 4728.0 73 3671.0 74 2652.0 75 2152.0 76 1676.0 77 849.5 78 342.0 79 185.0 80 123.5 81 44.5 82 18.5 83 10.0 84 9.5 85 9.5 86 9.0 87 8.0 88 5.0 89 3.0 90 6.5 91 6.5 92 4.0 93 5.5 94 8.0 95 7.0 96 5.0 97 6.0 98 7.5 99 23.5 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.838248233190475E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.838248233190475E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.838248233190475E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 176165.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.05683925275646 #Duplication Level Percentage of deduplicated Percentage of total 1 71.36371344969321 47.14061347826159 2 18.66598508803749 24.66031952909683 3 5.634071320391255 11.165068306489516 4 1.9176324104603248 5.066909435346142 5 0.811890753192279 2.681546848721087 6 0.4882035770071082 1.9349511125387557 7 0.2914927923432674 1.3478564769009433 8 0.22330459055318574 1.180063635405951 9 0.15907834024996986 0.9457391115438801 >10 0.44462767807189696 3.876932065695285 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.67649646638095E-4 0.0 3 0.0 0.0 0.0 5.67649646638095E-4 0.0 4 0.0 0.0 0.0 0.00681179575965714 0.0 5 0.0 0.0 0.0 0.009650043992847614 0.0 6 0.0 0.0 0.0 0.010217693639485709 0.0 7 0.0 0.0 0.0 0.010785343286123805 0.0 8 0.0 0.0 0.0 0.010785343286123805 0.0 9 0.0 0.0 0.0 0.014191241165952374 0.0 10 0.0 0.0 0.0 0.015894190105866658 0.0 11 0.0 0.0 0.0 0.016461839752504755 0.0 12 0.0 0.0 0.0 0.016461839752504755 0.0 13 0.0 0.0 0.0 0.01816478869241904 0.0 14 0.0 0.0 0.0 0.01816478869241904 0.0 15 0.0 0.0 0.0 0.019867737632333324 0.0 16 0.0 0.0 0.0 0.021003036925609515 0.0 17 0.0 0.0 0.0 0.023273635512161894 0.0 18 0.0 0.0 0.0 0.023841285158799987 0.0 19 0.0 0.0 0.0 0.03292367950500951 0.0 20 0.0 0.0 0.0 0.035761927738199986 0.0 21 0.0 0.0 0.0 0.038600175971390455 0.0 22 0.0 0.0 0.0 0.04654727102432379 0.0 23 0.0 0.0 0.0 0.051088468197428544 0.0 24 0.0 0.0 0.0 0.05562966537053331 0.0 25 0.0 0.0 0.0 0.06300911077682854 0.0 26 0.0 0.0 0.0 0.08117389946924758 0.0 27 0.0 0.0 0.0 0.21854511395566656 0.0 28 0.0 0.0 0.0 0.39224590582692365 0.0 29 0.0 0.0 0.0 0.5897879828569806 0.0 30 0.0 5.67649646638095E-4 0.0 0.7674623222547043 0.0 31 0.0 5.67649646638095E-4 0.0 0.9633014503448472 0.0 32 0.0 5.67649646638095E-4 0.0 1.183549513240428 0.0 33 0.0 5.67649646638095E-4 0.0 1.440694803167485 0.0 34 0.0 5.67649646638095E-4 0.0 1.8624584906195896 0.0 35 0.0 5.67649646638095E-4 0.0 2.492549598387875 0.0 36 0.0 5.67649646638095E-4 0.0 3.5194278091561886 0.0 37 0.0 5.67649646638095E-4 0.0 5.08046433741095 0.0 38 0.0 5.67649646638095E-4 0.0 6.837907643402492 0.0 39 0.0 5.67649646638095E-4 0.0 8.523259444270996 0.0 40 0.0 0.00113529929327619 0.0 10.265376209803309 0.0 41 0.0 0.00113529929327619 0.0 12.05347259671331 0.0 42 0.0 0.00113529929327619 0.0 13.949422416484547 0.0 43 0.0 0.00113529929327619 0.0 15.701756875656345 0.0 44 0.0 0.00113529929327619 0.0 17.2701728493174 0.0 45 0.0 0.00113529929327619 0.0 18.83858882297846 0.0 46 0.0 0.00113529929327619 0.0 20.400760650526497 0.0 47 0.0 0.00113529929327619 0.0 21.88913802401158 0.0 48 0.0 0.00113529929327619 0.0 23.317344534953026 0.0 49 0.0 0.00113529929327619 0.0 24.721709760735674 0.0 50 0.0 0.00113529929327619 0.0 26.14991627167712 0.0 51 0.0 0.00113529929327619 0.0 27.55314619816649 0.0 52 0.0 0.00113529929327619 0.0 28.9450231317231 0.0 53 0.0 0.00113529929327619 0.0 30.346550109272556 0.0 54 0.0 0.00113529929327619 0.0 31.648738398660345 0.0 55 0.0 0.00113529929327619 0.0 32.89189112479777 0.0 56 0.0 0.00113529929327619 0.0 34.027758067720605 0.0 57 0.0 0.00113529929327619 0.0 35.17668095251611 0.0 58 0.0 0.00113529929327619 0.0 36.274515369114184 0.0 59 0.0 0.00113529929327619 0.0 37.366673289245874 0.0 60 0.0 0.00113529929327619 0.0 38.39298385036755 0.0 61 0.0 0.00113529929327619 0.0 39.38239718445775 0.0 62 0.0 0.00113529929327619 0.0 40.34853688303579 0.0 63 0.0 0.00113529929327619 0.0 41.253938069423555 0.0 64 0.0 0.00113529929327619 0.0 42.17182754803735 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTTCG 15 0.0022200185 70.01391 27 TAGTTAG 20 0.006944656 52.49553 9 ATACGTA 20 0.006944656 52.49553 70 AGAGCGG 20 0.006944656 52.49553 5 TATGGCA 30 5.868245E-4 46.66269 48 CTTCCGA 40 4.5812034E-5 43.746277 18 GGATAAT 40 0.0024149036 34.99702 6 TGTAAGT 40 0.0024149036 34.99702 3 AGTTGAA 45 0.0043004514 31.108463 20 ACGCTTC 60 4.9698586E-4 29.16418 15 AGGTAGT 50 0.007197057 27.997616 6 TGGAATT 50 0.007197057 27.997616 23 CCCTATA 65 7.936411E-4 26.920784 2 GGGTTAG 75 0.0018278098 23.331347 1 CTAGGGA 75 0.0018278098 23.331347 4 TGACTCT 75 0.0018278098 23.331347 10 ACCCTAT 90 0.005262086 19.44279 1 AATATCT 90 0.005262086 19.44279 38 GGGACGA 90 0.005262086 19.44279 5 CTCCTAG 90 0.005262086 19.44279 1 >>END_MODULE