FastQCFastQC Report
Wed 25 May 2016
SRR1780298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176165
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT94925.388130445888797No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG25231.4321800584679136No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC23261.3203530780802089No Hit
CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG18211.033690006527971RNA PCR Primer, Index 32 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC6220.3530780802088951No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT3240.18391848551074277RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT2790.1583742514120285No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTAAG150.002221769470.000011
CTTAATA452.6861017E-854.4444432
CTCACTA200.006942318852.49999615
TTAATAC407.4478885E-752.4999963
TAAGTCC200.006942318852.4999964
CGATTAT200.006942318852.49999616
CGACAAC200.006942318852.49999614
GGCCAAT200.006942318852.49999630
GTAAGTC200.006942318852.4999963
TTTCTCG200.006942318852.49999639
TCTTTAT1000.042.01
CGTAGGA350.001252876640.0000042
ATAAGAG556.666978E-638.1818163
TAATACA654.83893E-737.692314
TATGCCG20000.035.743
TCGTATG19950.035.61403740
CGTATGC20050.035.61097341
CTCGTAT19700.035.53299339
CCGTCTT20050.035.4364147
GTATGCC20150.035.43424242