##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780298_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176165 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.71111741832941 34.0 34.0 34.0 34.0 34.0 2 33.69937842363693 34.0 34.0 34.0 34.0 34.0 3 33.69969630743905 34.0 34.0 34.0 34.0 34.0 4 33.73082053756421 34.0 34.0 34.0 34.0 34.0 5 33.72778928845117 34.0 34.0 34.0 34.0 34.0 6 37.44888031107201 38.0 38.0 38.0 37.0 38.0 7 37.45413107030341 38.0 38.0 38.0 37.0 38.0 8 37.34467686543865 38.0 38.0 38.0 36.0 38.0 9 37.36612266908864 38.0 38.0 38.0 37.0 38.0 10-11 37.317338858456566 38.0 38.0 38.0 36.0 38.0 12-13 37.30640308801408 38.0 38.0 38.0 36.0 38.0 14-15 37.224411773053674 38.0 38.0 38.0 36.0 38.0 16-17 37.27116055970255 38.0 38.0 38.0 36.0 38.0 18-19 37.22150256861465 38.0 38.0 38.0 36.0 38.0 20-21 37.24738171600488 38.0 38.0 38.0 36.0 38.0 22-23 37.214089064229555 38.0 38.0 38.0 36.0 38.0 24-25 37.23751880339454 38.0 38.0 38.0 36.0 38.0 26-27 37.12925950103596 38.0 38.0 38.0 36.0 38.0 28-29 37.07713507223342 38.0 38.0 38.0 36.0 38.0 30-31 37.053154712911194 38.0 38.0 38.0 36.0 38.0 32-33 37.005645275735816 38.0 38.0 38.0 36.0 38.0 34-35 37.005052081855084 38.0 38.0 38.0 35.5 38.0 36-37 37.032742031618085 38.0 38.0 38.0 36.0 38.0 38-39 36.44013283001731 38.0 37.0 38.0 34.0 38.0 40-41 36.72575142621974 38.0 37.0 38.0 34.5 38.0 42-43 36.94760877586354 38.0 37.5 38.0 35.0 38.0 44-45 37.02817529021088 38.0 38.0 38.0 36.0 38.0 46-47 36.97475662021401 38.0 38.0 38.0 35.5 38.0 48-49 37.01664632588766 38.0 38.0 38.0 36.0 38.0 50-51 36.88408026566003 38.0 37.5 38.0 35.0 38.0 52-53 36.90080038600176 38.0 38.0 38.0 35.0 38.0 54-55 36.880640308801404 38.0 37.5 38.0 35.0 38.0 56-57 36.91682513552635 38.0 38.0 38.0 35.0 38.0 58-59 36.807211988760535 38.0 37.5 38.0 35.0 38.0 60-61 36.81712598983907 38.0 37.0 38.0 35.0 38.0 62-63 36.76381233502683 38.0 37.0 38.0 34.5 38.0 64-65 36.72160474555105 38.0 37.0 38.0 34.5 38.0 66-67 36.45269207844918 38.0 37.0 38.0 34.0 38.0 68-69 36.37868475576874 38.0 37.0 38.0 34.0 38.0 70-71 35.77602815542247 38.0 37.0 38.0 31.0 38.0 72-73 34.865580563676104 38.0 36.5 38.0 24.0 38.0 74-75 34.59125535719354 38.0 36.5 38.0 23.0 38.0 76 33.551772486021626 37.0 34.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03358446029665174 1101 2 0.05613580401323759 1101 3 0.025319330167761223 1101 4 0.14877032389501466 1101 5 -0.14198389800854017 1101 6 -0.09033037236123675 1101 7 0.13707043876542002 1101 8 0.0064679785667891565 1101 9 0.06269353174279502 1101 10-11 0.08694951713652443 1101 12-13 0.2623612502902475 1101 14-15 0.311610247531533 1101 16-17 0.1560734378820925 1101 18-19 0.21770274460751438 1101 20-21 0.2045892816199668 1101 22-23 0.1772068737904462 1101 24-25 0.05163803215876328 1101 26-27 0.27751438450194854 1101 28-29 0.14963046053286178 1101 30-31 0.23001989681805668 1101 32-33 0.09414351266874377 1101 34-35 0.27732978892573357 1101 36-37 0.2019368621432065 1101 38-39 0.2173767229930803 1101 40-41 0.18748250675384526 1101 42-43 0.296086516378125 1101 44-45 0.09747883640603305 1101 46-47 0.20672765138181148 1101 48-49 0.23649358762268946 1101 50-51 0.04712176574982152 1101 52-53 0.19717838232048734 1101 54-55 0.10633643973745421 1101 56-57 0.22180807179856998 1101 58-59 0.32191501035352843 1101 60-61 0.26263748166388723 1101 62-63 0.2514189987631781 1101 64-65 0.24779308886623141 1101 66-67 0.3010731750937481 1101 68-69 -0.28157686967437456 1101 70-71 -0.22782659420111884 1101 72-73 -0.36434127779816805 1101 74-75 -0.23455726333598648 1101 76 -0.9091920881197666 1102 1 0.043549236399584856 1102 2 0.0027803369517229726 1102 3 -0.21117740955246944 1102 4 -0.06470672539084887 1102 5 0.05093361553380049 1102 6 0.17531575163056345 1102 7 0.026929083335140547 1102 8 -0.15157051940530408 1102 9 -0.1461023260004879 1102 10-11 -0.011284229748035557 1102 12-13 0.02614501418896964 1102 14-15 0.03257812296953233 1102 16-17 -0.006965841821397589 1102 18-19 0.06561920051326098 1102 20-21 0.11636169661817775 1102 22-23 0.06744258879876952 1102 24-25 -0.002216363470900262 1102 26-27 0.1514027164710754 1102 28-29 0.18680178846458517 1102 30-31 0.20492401976141394 1102 32-33 0.2423574863473874 1102 34-35 0.16235779330343547 1102 36-37 0.2483878016004084 1102 38-39 0.16121621974200195 1102 40-41 0.2928644975018315 1102 42-43 0.2749176899868715 1102 44-45 0.2951741083106114 1102 46-47 0.2355873351944524 1102 48-49 0.0969683285017311 1102 50-51 -0.02214423445251157 1102 52-53 0.1325062036810607 1102 54-55 0.1710936891465593 1102 56-57 0.3037519158937343 1102 58-59 0.2694148494088253 1102 60-61 0.27484628671847133 1102 62-63 0.40363819624692354 1102 64-65 0.41454099428592883 1102 66-67 0.20463281200250805 1102 68-69 -0.12520299973608928 1102 70-71 0.10236065346058609 1102 72-73 -0.08563564105495658 1102 74-75 0.011016757430475366 1102 76 -0.04735621492579867 1103 1 -0.06715367392436633 1103 2 -0.006483486979895758 1103 3 0.035314897744129325 1103 4 0.09115786245257596 1103 5 0.10183106187349722 1103 6 0.07074299781850613 1103 7 0.08885652457352222 1103 8 -0.1813853715313556 1103 9 -0.1786564578545793 1103 10-11 0.08158764140721786 1103 12-13 0.1312836286021266 1103 14-15 0.17295227685789882 1103 16-17 0.14755911391715415 1103 18-19 0.14825842579865878 1103 20-21 0.0015820178726713152 1103 22-23 0.07699791667640454 1103 24-25 0.1396646086090314 1103 26-27 0.09119429114967659 1103 28-29 0.07123762115846688 1103 30-31 0.18840260493843886 1103 32-33 0.1499807432180944 1103 34-35 0.00937395033832189 1103 36-37 0.07924692374484721 1103 38-39 0.15173162443949906 1103 40-41 0.2886548375754714 1103 42-43 -0.15690039440352166 1103 44-45 -0.035706447947504216 1103 46-47 0.03253340267761473 1103 48-49 0.12179331896354739 1103 50-51 -0.10740699525209152 1103 52-53 -0.009743435154121016 1103 54-55 -0.06030685870938868 1103 56-57 -0.16174130449611113 1103 58-59 0.2349852326079116 1103 60-61 0.05282102018526302 1103 62-63 0.05335976711024415 1103 64-65 0.35348377806287345 1103 66-67 -0.16438331931144745 1103 68-69 0.20177440442126482 1103 70-71 -0.06579211175441912 1103 72-73 0.22395441008684003 1103 74-75 0.22632885955974302 1103 76 0.47545182543893105 1104 1 0.02053391119960679 1104 2 0.09072132286303969 1104 3 0.07700325757436133 1104 4 0.10179549568331225 1104 5 0.1546402050733704 1104 6 0.1517414525710734 1104 7 0.061975050465783 1104 8 -7.788515035329624E-4 1104 9 0.031901205697991486 1104 10-11 -0.006162420027827409 1104 12-13 -0.054779155925409384 1104 14-15 0.10105453808385079 1104 16-17 0.23658160955422858 1104 18-19 0.15193619797670266 1104 20-21 0.06043678543996833 1104 22-23 0.05302783866432037 1104 24-25 0.0011671222392735103 1104 26-27 0.20908500758635995 1104 28-29 0.02150856009411939 1104 30-31 0.023219403568958796 1104 32-33 0.22617476964973804 1104 34-35 0.23511544002348472 1104 36-37 0.019888180601626004 1104 38-39 0.2586639959153132 1104 40-41 0.00972518173664838 1104 42-43 -0.07019785245067567 1104 44-45 -0.03163715755344754 1104 46-47 0.028076759154949116 1104 48-49 0.12314905491366801 1104 50-51 -0.012590863035555344 1104 52-53 -0.06015557055278009 1104 54-55 -0.03952723764903254 1104 56-57 -0.2326914858856597 1104 58-59 0.04838515180521341 1104 60-61 -0.0032389737337013003 1104 62-63 0.0549570781298101 1104 64-65 -0.023158828282220156 1104 66-67 -0.20027387747480674 1104 68-69 -0.01076792327948084 1104 70-71 -0.12772696145724183 1104 72-73 0.2249082151024382 1104 74-75 0.14362271897138612 1104 76 0.32447573720571654 1105 1 0.11142013953450203 1105 2 0.16140603072431503 1105 3 0.17412309794112701 1105 4 0.12375331161035774 1105 5 0.1340717590237972 1105 6 0.18749814380938545 1105 7 0.08334743457502469 1105 8 0.050726780185641474 1105 9 0.14452262095879576 1105 10-11 0.18446572357613178 1105 12-13 0.25674271943533 1105 14-15 0.26433848076100475 1105 16-17 0.14415140786883995 1105 18-19 0.3813430153714492 1105 20-21 0.23483980989617947 1105 22-23 0.1775784579027686 1105 24-25 0.16934065742290016 1105 26-27 0.2961741746240776 1105 28-29 0.3784591199648304 1105 30-31 0.23271947777862323 1105 32-33 0.28254113453042606 1105 34-35 0.28442570239588605 1105 36-37 0.3259206019806058 1105 38-39 0.3760162774503044 1105 40-41 0.3384307177778112 1105 42-43 0.2364674124778361 1105 44-45 0.14214951439034706 1105 46-47 0.1846385428255246 1105 48-49 0.1488400293690546 1105 50-51 0.18128601488129448 1105 52-53 0.4689943989388041 1105 54-55 0.18468696443213162 1105 56-57 0.38453505149127665 1105 58-59 0.5083696502303212 1105 60-61 0.5329403003308499 1105 62-63 0.22627215588504868 1105 64-65 0.34402442012227397 1105 66-67 0.08884492395660715 1105 68-69 0.3994651775302387 1105 70-71 0.4275448108345188 1105 72-73 0.7759026246408567 1105 74-75 0.802592194061674 1105 76 0.7328515349596358 1106 1 0.10246875410228284 1106 2 0.06486546327782605 1106 3 0.09608261345464797 1106 4 -0.01777647103440927 1106 5 -0.14405168547595082 1106 6 0.0023065061787264085 1106 7 -0.18793180882965999 1106 8 0.009470765584680407 1106 9 0.13849142799380587 1106 10-11 0.13234194723631987 1106 12-13 0.1254377584265356 1106 14-15 -0.10709423866823187 1106 16-17 -0.1397999775633778 1106 18-19 0.1429657132310851 1106 20-21 0.1316997733937697 1106 22-23 -0.04526458175212156 1106 24-25 0.21999189352710147 1106 26-27 -0.02245864435717948 1106 28-29 0.020200450643443446 1106 30-31 0.04306044343318405 1106 32-33 -0.05607675164843329 1106 34-35 0.11324186300647199 1106 36-37 0.3182633927554477 1106 38-39 0.22236188886559205 1106 40-41 0.2682341025463515 1106 42-43 0.2055938435380682 1106 44-45 0.34287126650583133 1106 46-47 0.27212817282386936 1106 48-49 0.302577377967026 1106 50-51 0.21529132432215192 1106 52-53 0.2480594395892055 1106 54-55 0.17055472617096967 1106 56-57 0.025525594049760514 1106 58-59 0.1951956001224744 1106 60-61 0.18329288038725622 1106 62-63 0.37304086336804687 1106 64-65 0.2553360179321231 1106 66-67 0.13184724683698335 1106 68-69 0.3035260701799771 1106 70-71 0.18774474446650657 1106 72-73 0.39892402832428075 1106 74-75 0.5847226072025364 1106 76 0.926032221868546 1107 1 0.06761229579569061 1107 2 0.00986182660302859 1107 3 -0.007844995324717274 1107 4 0.09938863400131481 1107 5 0.0101647180665978 1107 6 0.01437598805952689 1107 7 0.10231762427692104 1107 8 0.2768989947379765 1107 9 0.0980558827065039 1107 10-11 0.01820811760384089 1107 12-13 0.11251686679944584 1107 14-15 0.10917025455044183 1107 16-17 0.10112972765203665 1107 18-19 0.06994556177427569 1107 20-21 0.1461822959625465 1107 22-23 0.05085273309835259 1107 24-25 0.05748173222988129 1107 26-27 0.0792441751354005 1107 28-29 0.08212660475287947 1107 30-31 -0.022515220794780078 1107 32-33 0.1511559527540811 1107 34-35 -0.07060702245966155 1107 36-37 -0.0721721525318415 1107 38-39 0.0768970362578898 1107 40-41 0.17444212034466489 1107 42-43 -0.0176423691860208 1107 44-45 0.1466619651752339 1107 46-47 0.016450681701627445 1107 48-49 0.05866132097818877 1107 50-51 0.04547786434696377 1107 52-53 0.08757725929570626 1107 54-55 -0.0447385609552029 1107 56-57 -0.16286485647007254 1107 58-59 0.08192387633862097 1107 60-61 -0.3956850000731009 1107 62-63 -0.07168381895771603 1107 64-65 0.21995758983132419 1107 66-67 0.2157496128148253 1107 68-69 0.08431587037678412 1107 70-71 0.09470362864911408 1107 72-73 0.02664404714942492 1107 74-75 0.05223426595408398 1107 76 -0.22954044745075208 1108 1 -0.22910387494933815 1108 2 -0.1861549448854376 1108 3 -0.03368929530886078 1108 4 0.07796182428371878 1108 5 -8.944225707949727E-4 1108 6 -0.020382569451527388 1108 7 0.0467620687213639 1108 8 0.03660161792561922 1108 9 0.027627082255506252 1108 10-11 0.07874050666008969 1108 12-13 -0.02942418635137045 1108 14-15 0.06997658966309928 1108 16-17 0.175476132880803 1108 18-19 0.0705467456440445 1108 20-21 -0.003204188190863988 1108 22-23 0.012568551501175307 1108 24-25 0.056006299289400374 1108 26-27 0.14482827040873048 1108 28-29 0.18124486841055187 1108 30-31 0.07838457308503877 1108 32-33 0.062200559275936484 1108 34-35 0.01858399986121384 1108 36-37 0.23555030752135764 1108 38-39 0.18625744708889158 1108 40-41 -0.003314516901433251 1108 42-43 0.017928777511656335 1108 44-45 -0.02417555222165646 1108 46-47 0.20556154617320033 1108 48-49 0.05776945479775719 1108 50-51 -0.092241688883135 1108 52-53 0.14742918748837042 1108 54-55 -0.07980541788665363 1108 56-57 -0.05310585853127492 1108 58-59 -0.06440824141414225 1108 60-61 0.19760860667777536 1108 62-63 0.1114262175097096 1108 64-65 0.25965774662360275 1108 66-67 0.11840186868572289 1108 68-69 0.08731958758693992 1108 70-71 0.5273005667954394 1108 72-73 0.5169612432102397 1108 74-75 0.4548006384150227 1108 76 1.550642065806123 1109 1 -0.04730279630892653 1109 2 0.019601984752085855 1109 3 0.0893509655716116 1109 4 0.06400848175267271 1109 5 0.15917706871228887 1109 6 -0.3040900823074111 1109 7 -0.005476737248784502 1109 8 -0.2072212843696306 1109 9 0.014488991198192025 1109 10-11 -0.2013924420417723 1109 12-13 -0.041025841938981955 1109 14-15 -0.06726786035571308 1109 16-17 -0.14810031262277334 1109 18-19 0.012993127181736952 1109 20-21 0.0014835077830497312 1109 22-23 -0.07129260490167866 1109 24-25 -0.004604512199371413 1109 26-27 -0.044968543839353003 1109 28-29 -0.07684493467485964 1109 30-31 -0.028429047885730085 1109 32-33 0.05575693705092277 1109 34-35 -0.12041637640869851 1109 36-37 -0.18025929990370315 1109 38-39 -0.2771659819842327 1109 40-41 -0.06094652492851793 1109 42-43 -0.10835681135075959 1109 44-45 0.05807077914671055 1109 46-47 -0.028039356874586474 1109 48-49 -0.22789779717766123 1109 50-51 -0.1100361200884521 1109 52-53 0.011705495026305357 1109 54-55 -0.3646417404753848 1109 56-57 0.10251310547223369 1109 58-59 -0.060363339871436494 1109 60-61 0.004660531925480882 1109 62-63 -0.28110168917535816 1109 64-65 -0.10588847186379269 1109 66-67 0.08312656453421141 1109 68-69 0.09697718196797922 1109 70-71 0.20627962110373232 1109 72-73 -0.012528911060421422 1109 74-75 0.1651825523746311 1109 76 0.19676021455816084 1110 1 -0.022103332796994835 1110 2 -0.07123559004672586 1110 3 0.021864377773276544 1110 4 0.14330812409396287 1110 5 0.09488017759185396 1110 6 -0.18248612054938462 1110 7 -0.16493147966573218 1110 8 0.04353100258270359 1110 9 -0.10767381229585737 1110 10-11 -0.1520639118815197 1110 12-13 -0.056404736636004316 1110 14-15 -0.06629848488284296 1110 16-17 0.04591019360186266 1110 18-19 0.06402397524556847 1110 20-21 0.10193101105011948 1110 22-23 -0.0776913427791115 1110 24-25 -0.023478317137808347 1110 26-27 0.08702127664348325 1110 28-29 0.08429278112402727 1110 30-31 -0.10525259145819632 1110 32-33 -0.13914126777747526 1110 34-35 -0.2624288084879325 1110 36-37 -0.04604700882453727 1110 38-39 0.22597857747652483 1110 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0.01910282936684382 2115 62-63 0.09048346540885888 2115 64-65 -0.06905811472093148 2115 66-67 0.10684724683698477 2115 68-69 0.0295464783432422 2115 70-71 0.010034285282834787 2115 72-73 0.34659622220183195 2115 74-75 0.15831954597804554 2115 76 0.42367252799099475 2116 1 0.02010590070404561 2116 2 -0.05312742194966091 2116 3 -0.054582056082892905 2116 4 -0.04089948170656754 2116 5 -0.12017975138121528 2116 6 -0.039174489889425956 2116 7 0.1453859219323732 2116 8 0.025422834485638646 2116 9 0.11757774141825905 2116 10-11 0.13713037636944847 2116 12-13 0.14090770787421292 2116 14-15 0.19310703450158684 2116 16-17 -0.13638301444785839 2116 18-19 0.0028187370093988307 2116 20-21 -0.009612714904299935 2116 22-23 0.1009580357352391 2116 24-25 0.017815334830224572 2116 26-27 0.2339212470489329 2116 28-29 -0.0018366210659692683 2116 30-31 -0.08751435529365637 2116 32-33 0.09254710156257318 2116 34-35 -0.06337768389486342 2116 36-37 0.023037779574401895 2116 38-39 -0.11283283122427434 2116 40-41 0.00563814673282792 2116 42-43 0.2064551054740349 2116 44-45 0.02265969563895709 2116 46-47 -0.10697218291475963 2116 48-49 -0.08447092759372765 2116 50-51 0.15847144040527894 2116 52-53 -0.2184543218786814 2116 54-55 -0.08456603773091587 2116 56-57 -0.10310200377836765 2116 58-59 -0.16765779811755976 2116 60-61 -0.20596474927010888 2116 62-63 0.02940671245998061 2116 64-65 -0.2243353919162132 2116 66-67 -0.22654069549591327 2116 68-69 -0.19063700789529037 2116 70-71 -0.04620957120471303 2116 72-73 -0.7576899016513821 2116 74-75 -0.4191296676523564 2116 76 -0.559719510016869 2117 1 0.00821327413251538 2117 2 0.003659869929379056 2117 3 -0.002254499168181212 2117 4 -0.016264755236981898 2117 5 0.02200084664800528 2117 6 0.07333825221046908 2117 7 -0.04583052387123132 2117 8 -0.4387718648008061 2117 9 -0.06996473224806721 2117 10-11 -0.10307944354220666 2117 12-13 -6.251667435250852E-4 2117 14-15 0.03966029647916258 2117 16-17 0.04325935728166996 2117 18-19 0.006004261983918013 2117 20-21 0.0029913455782022425 2117 22-23 0.13176206223880627 2117 24-25 -0.04923996588333068 2117 26-27 0.09269630651206029 2117 28-29 -0.7879817111101488 2117 30-31 -0.02535414462426644 2117 32-33 -0.09866934424103135 2117 34-35 0.07400338983942589 2117 36-37 0.08272389918024459 2117 38-39 0.06648036959121839 2117 40-41 -0.3302966986630267 2117 42-43 -0.015480419500484288 2117 44-45 0.022033209060630554 2117 46-47 0.040188452491285886 2117 48-49 0.0873562895316482 2117 50-51 0.20646668335464824 2117 52-53 0.030929809959580723 2117 54-55 0.07995570878624392 2117 56-57 -0.034305282745400234 2117 58-59 0.10753970443086303 2117 60-61 -0.01524736904585211 2117 62-63 0.23437400773690342 2117 64-65 -0.016437413662728773 2117 66-67 0.21994248493222557 2117 68-69 0.028389070935837424 2117 70-71 0.11721847840452426 2117 72-73 -0.2266097963166871 2117 74-75 0.08457615973465948 2117 76 -0.7005834243899542 2118 1 0.06077380733086102 2118 2 -0.31257863509044626 2118 3 -0.32782608442085603 2118 4 -0.18208775363400775 2118 5 0.03285780269288807 2118 6 -0.044195283603244206 2118 7 0.006898742981043426 2118 8 0.07302894512073266 2118 9 -0.03387154908769219 2118 10-11 0.035087054390800176 2118 12-13 -0.10827987441247444 2118 14-15 -0.05013500850692054 2118 16-17 -0.09755414984732624 2118 18-19 -0.3301131766392089 2118 20-21 -0.18548678232190952 2118 22-23 0.05886121435219849 2118 24-25 0.07299965699695576 2118 26-27 -0.15473050526554744 2118 28-29 -0.07197099652312744 2118 30-31 -0.05081710758722835 2118 32-33 -0.025438938436593617 2118 34-35 -0.22712818022722558 2118 36-37 0.08177740884024587 2118 38-39 -0.06521888376235552 2118 40-41 0.10095224675163905 2118 42-43 -0.014428470626782541 2118 44-45 -0.07226517531463372 2118 46-47 0.06687737090855705 2118 48-49 -0.12614978487988537 2118 50-51 -0.20753188240929177 2118 52-53 -0.014696824647693063 2118 54-55 0.04913545231108429 2118 56-57 -0.12796564299175373 2118 58-59 -0.1182021963537494 2118 60-61 -0.31824819162241624 2118 62-63 0.0025629683375925083 2118 64-65 -0.30032387700534713 2118 66-67 -0.1460408104360411 2118 68-69 -0.20194554899160977 2118 70-71 -0.18932047797782303 2118 72-73 0.11743548757768707 2118 74-75 0.059342569082502905 2118 76 -0.5485807668349096 2119 1 0.09005735597659736 2119 2 -8.402595707508453E-4 2119 3 -0.07891857583225459 2119 4 0.016498777526550157 2119 5 -0.059017671870513766 2119 6 -0.14218233831012128 2119 7 -0.18051065855136272 2119 8 -0.0291953302242689 2119 9 -0.1587208835042233 2119 10-11 -0.014323292286057665 2119 12-13 -0.12074463061299667 2119 14-15 -0.5050415294726633 2119 16-17 -0.18985706333475605 2119 18-19 -0.14978439380473674 2119 20-21 0.08748208631894272 2119 22-23 -0.09067869145194152 2119 24-25 -0.07354552143888071 2119 26-27 -0.19589473207897612 2119 28-29 0.15707785894942816 2119 30-31 -0.031003587773710706 2119 32-33 -0.5270557726274561 2119 34-35 -0.18054530220926068 2119 36-37 -0.07253866233231321 2119 38-39 0.03416199352284366 2119 40-41 -0.13764097475729642 2119 42-43 -0.29682754459761185 2119 44-45 -0.11276833199091385 2119 46-47 -0.21360752005468697 2119 48-49 -0.11005047037510707 2119 50-51 0.035882637770605186 2119 52-53 -0.10357226204249059 2119 54-55 -0.1058284144978856 2119 56-57 0.004439537249417924 2119 58-59 -0.028556837915679978 2119 60-61 0.042712032663551724 2119 62-63 -0.30315674854384866 2119 64-65 -0.3684592344345532 2119 66-67 -0.3756286106388771 2119 68-69 0.11958144337820897 2119 70-71 0.11445850689455739 2119 72-73 -0.048490461947352514 2119 74-75 0.06001160392010263 2119 76 -1.088258249564781 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 2.0 21 6.0 22 5.0 23 16.0 24 26.0 25 84.0 26 147.0 27 335.0 28 530.0 29 895.0 30 1485.0 31 2381.0 32 3728.0 33 5976.0 34 9639.0 35 16131.0 36 25523.0 37 109255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46663639201885 12.772117049357137 9.756194476768938 34.005052081855084 2 24.273266539891576 15.855022280248631 30.89035847075185 28.981352709107938 3 22.459625918882868 14.798626287855136 28.316067323248088 34.42568047001391 4 17.672068799137172 18.353816024749527 26.998552493401075 36.97556268271223 5 19.17179916555502 26.78511622626515 33.46464961825561 20.57843498992422 6 35.36343768626004 31.48695824936849 14.431356966480289 18.71824709789118 7 33.549797065251326 21.672863508642465 16.97556268271223 27.80177674339398 8 28.62600403031249 32.609769250418644 13.949422416484547 24.81480430278432 9 24.467970368688448 14.371753753583288 14.280362160474555 46.879913717253714 10-11 30.304260210598017 22.054607896006583 20.233587829591578 27.40754406380382 12-13 28.54795220389975 16.11159992052905 22.726988902449406 32.61345897312179 14-15 25.531461981664915 15.68784945931371 17.80631794056708 40.974370618454294 16-17 31.402094627196092 21.190928958646722 16.73147333465785 30.675503079499332 18-19 32.597280958192606 19.664519058836884 16.95824936848977 30.779950614480743 20-21 28.25163908835467 18.273209774926915 17.295149433769478 36.180001702948935 22-23 27.57727130814861 21.9549853830216 17.033179121845997 33.43456418698379 24-25 31.433315357761188 21.342207589475777 17.35106292396333 29.873414128799702 26-27 31.21987909062527 23.261714869582494 17.605369965657196 27.913036074135043 28-29 27.97689665938183 22.34183861720546 18.424772230579286 31.25649249283342 30-31 27.286067039423266 19.47861379956291 21.618936792211844 31.616382368801975 32-33 26.119547015581983 21.568132148837737 19.737178213606562 32.57514262197372 34-35 26.543297476797324 18.392132375897596 22.68157693071836 32.382993216586726 36-37 30.498964039394888 19.79167257968382 19.99063378083047 29.718729600090825 38-39 26.26628444923793 19.50415803366162 24.181591121959524 30.047966395140918 40-41 25.13382340419493 16.34149802741748 25.89702835409985 32.62765021428774 42-43 28.047001390741634 15.824936848976812 26.03581869270286 30.09224306757869 44-45 23.030113813754152 20.260551187806886 29.092612039849 27.616722958589957 46-47 25.21073993131439 17.983992279964806 27.58039338120512 29.22487440751568 48-49 24.326057957028922 18.002157068657223 24.890869355433825 32.78091561888003 50-51 21.726790225073085 19.44597394488122 27.71237192404848 31.11486390599722 52-53 18.540288933670137 19.602929072176654 33.055090398206225 28.80169159594698 54-55 18.403769193653677 19.649192518377657 29.95174978003576 31.995288507932905 56-57 21.467941986206114 20.198677376323335 25.752277694207137 32.58110294326342 58-59 19.456475463344024 20.516277353617348 29.743138534896264 30.284108648142364 60-61 22.587063264553116 23.69454772514404 24.706667045099763 29.011721965203073 62-63 18.89762438622882 29.718161950444188 21.150909658558735 30.233304004768257 64-65 18.236312547895437 31.73814321800585 19.613430590639457 30.412113643459254 66-67 18.614934862203047 31.535208469332726 18.98759685522096 30.862259813243263 68-69 18.693554338262423 32.629069338404335 18.716827973774585 29.960548349558653 70-71 18.65836006017086 32.1888570374365 18.747197229869723 30.405585672522918 72-73 18.03366162404564 30.54182158771606 19.646070445321147 31.77844634291715 74-75 17.633468623165783 29.468679930746745 20.278999801322623 32.61885164476485 76 17.50574745267221 29.16072999744558 20.429710782505037 32.90381176737717 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.5 16 2.5 17 3.0 18 6.0 19 16.0 20 18.5 21 14.0 22 17.0 23 33.0 24 45.5 25 45.0 26 94.0 27 174.0 28 257.5 29 310.0 30 383.5 31 528.5 32 737.5 33 875.0 34 1028.5 35 1378.0 36 1665.5 37 1757.0 38 2019.5 39 2784.0 40 5602.0 41 9029.0 42 10140.0 43 9491.5 44 8052.5 45 6592.5 46 5923.0 47 5620.0 48 5000.0 49 4482.0 50 4281.0 51 4120.5 52 3658.0 53 3240.5 54 3125.0 55 3259.5 56 3550.5 57 4147.0 58 4587.0 59 5130.5 60 6327.0 61 7488.0 62 7996.0 63 8484.5 64 9035.0 65 8934.0 66 8578.0 67 8385.0 68 7878.5 69 7069.5 70 6113.5 71 5460.0 72 4893.0 73 3686.0 74 2597.0 75 2148.0 76 1657.5 77 820.5 78 311.5 79 149.0 80 89.5 81 19.0 82 8.0 83 8.0 84 4.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 176165.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.97913887548606 #Duplication Level Percentage of deduplicated Percentage of total 1 66.14801660230863 36.36760991116283 2 21.82769942387511 24.00136235915193 3 6.779276023705784 11.181562739477195 4 2.2879798459537035 5.031646467800074 5 0.9581431845871106 2.633894360400761 6 0.5441179507299647 1.7949081826696565 7 0.3469139116608504 1.3351119688927995 8 0.24882813306626467 1.094428518718247 9 0.19926900282900037 0.986007436210371 >10 0.6370413199248354 4.911872392359435 >50 0.01032481879943007 0.4018959498197712 >100 0.007227373159601049 0.7322680441631425 >500 0.0010324818799430072 0.3530780802088951 >1k 0.0030974456398290212 3.7862231430760938 >5k 0.0010324818799430072 5.388130445888797 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 9492 5.388130445888797 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 2523 1.4321800584679136 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 2326 1.3203530780802089 No Hit CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG 1821 1.033690006527971 RNA PCR Primer, Index 32 (96% over 26bp) TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 622 0.3530780802088951 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT 324 0.18391848551074277 RNA PCR Primer, Index 42 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT 279 0.1583742514120285 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.67649646638095E-4 0.0 3 0.0 0.0 0.0 5.67649646638095E-4 0.0 4 0.0 0.0 0.0 0.00567649646638095 0.0 5 0.0 0.0 0.0 0.008514744699571425 0.0 6 0.0 0.0 0.0 0.00908239434620952 0.0 7 0.0 0.0 0.0 0.009650043992847614 0.0 8 0.0 0.0 0.0 0.009650043992847614 0.0 9 0.0 0.0 0.0 0.013055941872676185 0.0 10 0.0 0.0 0.0 0.015326540459228565 0.0 11 0.0 0.0 0.0 0.015894190105866658 0.0 12 0.0 0.0 0.0 0.015894190105866658 0.0 13 0.0 0.0 0.0 0.017597139045780943 0.0 14 0.0 0.0 0.0 0.017597139045780943 0.0 15 0.0 0.0 0.0 0.018732438339057134 0.0 16 0.0 0.0 0.0 0.019867737632333324 0.0 17 0.0 0.0 0.0 0.022138336218885703 0.0 18 0.0 0.0 0.0 0.0227059858655238 0.0 19 0.0 0.0 0.0 0.031788380211733316 0.0 20 0.0 0.0 0.0 0.03462662844492379 0.0 21 0.0 0.0 0.0 0.03746487667811427 0.0 22 0.0 0.0 0.0 0.0448443220844095 0.0 23 0.0 0.0 0.0 0.049953168904152356 0.0 24 0.0 0.0 0.0 0.053359066783980925 0.0 25 0.0 0.0 0.0 0.06187381148355235 0.0 26 0.0 0.0 0.0 0.08060624982260949 0.0 27 0.0 0.0 0.0 0.21911276360230467 0.0 28 0.0 0.0 0.0 0.39621945335339026 0.0 29 0.0 0.0 0.0 0.6000056764964664 0.0 30 0.0 0.0 0.0 0.7839241620072092 0.0 31 0.0 0.0 0.0 0.9831691879771804 0.0 32 0.0 0.0 0.0 1.2102290466324184 0.0 33 0.0 0.0 0.0 1.4730508330258565 0.0 34 0.0 0.0 0.0 1.9078704623506373 0.0 35 0.0 0.0 0.0 2.5442057162319416 0.0 36 0.0 0.0 0.0 3.5898163653393125 0.0 37 0.0 0.0 0.0 5.169585331933131 0.0 38 0.0 0.0 0.0 6.959952317429682 0.0 39 0.0 0.0 0.0 8.674254250276729 0.0 40 0.0 0.0 0.0 10.444753498140948 0.0 41 0.0 0.0 0.0 12.268044163142509 0.0 42 0.0 0.0 0.0 14.178752873726337 0.0 43 0.0 0.0 0.0 15.954928618056936 0.0 44 0.0 0.0 0.0 17.546050577583514 0.0 45 0.0 0.0 0.0 19.127522493117247 0.0 46 0.0 0.0 0.0 20.709562058297617 0.0 47 0.0 0.0 0.0 22.22632191411461 0.0 48 0.0 0.0 0.0 23.67950500950813 0.0 49 0.0 0.0 0.0 25.118496863735704 0.0 50 0.0 0.0 0.0 26.559759316549826 0.0 51 0.0 0.0 0.0 28.007833565123605 0.0 52 0.0 0.0 0.0 29.423551783839013 0.0 53 0.0 0.0 0.0 30.848352396900633 0.0 54 0.0 0.0 0.0 32.17494962109386 0.0 55 0.0 0.0 0.0 33.42775239122414 0.0 56 0.0 0.0 0.0 34.590298867538955 0.0 57 0.0 0.0 0.0 35.74887179632731 0.0 58 0.0 0.0 0.0 36.85919450515142 0.0 59 0.0 0.0 0.0 37.94908182669656 0.0 60 0.0 0.0 0.0 38.98788068004428 0.0 61 0.0 0.0 0.0 39.99602645247354 0.0 62 0.0 0.0 0.0 40.97465444327761 0.0 63 0.0 0.0 0.0 41.88629977577839 0.0 64 0.0 0.0 0.0 42.82292169273125 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTAAG 15 0.0022217694 70.00001 1 CTTAATA 45 2.6861017E-8 54.444443 2 CTCACTA 20 0.0069423188 52.499996 15 TTAATAC 40 7.4478885E-7 52.499996 3 TAAGTCC 20 0.0069423188 52.499996 4 CGATTAT 20 0.0069423188 52.499996 16 CGACAAC 20 0.0069423188 52.499996 14 GGCCAAT 20 0.0069423188 52.499996 30 GTAAGTC 20 0.0069423188 52.499996 3 TTTCTCG 20 0.0069423188 52.499996 39 TCTTTAT 100 0.0 42.0 1 CGTAGGA 35 0.0012528766 40.000004 2 ATAAGAG 55 6.666978E-6 38.181816 3 TAATACA 65 4.83893E-7 37.69231 4 TATGCCG 2000 0.0 35.7 43 TCGTATG 1995 0.0 35.614037 40 CGTATGC 2005 0.0 35.610973 41 CTCGTAT 1970 0.0 35.532993 39 CCGTCTT 2005 0.0 35.43641 47 GTATGCC 2015 0.0 35.434242 42 >>END_MODULE