FastQCFastQC Report
Wed 25 May 2016
SRR1780297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740824
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTG250.002345462335.22060436
ATAAGCC502.3752073E-630.7743473
GAATTGG300.00572719129.350537
TGTGTTC300.00572719129.350537
GTATAAG1800.026.8664931
GCATAAG701.1395241E-625.1219161
TATAAGA1850.024.9521712
TTTTCAC551.5785101E-424.03191620
CAGATTA551.5799594E-424.02866415
GACAATT651.8039706E-523.70617534
TAAGAGA1950.023.6725734
TAGCCCG752.0774696E-623.447125
AAGAGAC2000.023.0807615
ACGAAAT500.002583896321.99355130
AGAGACA2100.021.9816746
ATAAGAG2150.021.4704723
CGTCAGA755.452884E-520.54118512
CATAAGC755.5041644E-520.516232
TTCGGTA654.939435E-420.3017426
GGCTTAT654.957459E-420.2907771
Produced by FastQC (version 0.11.5)