FastQCFastQC Report
Wed 25 May 2016
SRR1780297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740824
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT202932.7392471086249905No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG56610.7641491096400764No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC55730.7522704448020043No Hit
CACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTG29350.396180469315249Illumina PCR Primer Index 4 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC11840.15982203600315323No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT250.002352826335.19811233
ATGCCGT27300.034.16666444
CTTTATA1650.032.00262
TCTTTAT1700.031.1180651
ATATCGT502.3608118E-630.79834737
TATATCG651.745866E-830.45990236
TTAATAC454.0651867E-529.3357183
TCGTTTA300.005745104529.33175532
CCTAACT300.005745104529.3317558
TCTTCTG3950.028.9604744
TATGCCG32800.028.37042843
TTTATAC2050.027.9047093
TTGCGAG407.0317497E-427.4985269
TACCGAA508.3401945E-526.39858211
GTCTTCT4400.025.99860643
ATCGTAT609.750391E-625.66528539
TATCGTA609.750391E-625.66528538
CCGTCTT4500.024.93199341
TTACTCG551.5933858E-423.99871319
CGTCTTC4750.023.61978342
Produced by FastQC (version 0.11.5)