FastQCFastQC Report
Wed 25 May 2016
SRR1780296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143037
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGAG150.00222091270.054
TTGGCAA200.00693965852.528
ATAGACA200.00693965852.527
ATGTAGG200.00693965852.51
GTAGCAA200.00693965852.535
CGATATG200.00693965852.565
ACGAATT200.00693965852.524
GTTTATC400.002412380235.038
GTAATAC400.002412380235.03
TGCTTAC450.00429597931.1111097
GCCGTAT450.00429597931.11110954
TTTTAGT450.00429597931.11110936
CCGTATC604.962758E-429.16666655
TCTACGA500.00718960528.0000022
TGTATAT750.001825230223.33333438
AGAGCAC750.001825230223.33333418
TAGATCT1202.1469295E-623.33333239
AGCACAC952.7649442E-422.10526320
AGATCTC1355.9641206E-620.74074240
GTATTAG850.003774794520.5882361
Produced by FastQC (version 0.11.5)