##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780296_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 143037 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.39909953368709 34.0 32.0 34.0 12.0 34.0 2 30.51502058907835 34.0 32.0 34.0 12.0 34.0 3 30.24285324776107 34.0 31.0 34.0 11.0 34.0 4 30.140732817382915 34.0 31.0 34.0 11.0 34.0 5 30.140201486328714 34.0 31.0 34.0 11.0 34.0 6 33.01396142256898 37.0 34.0 38.0 11.0 38.0 7 33.48662234247083 38.0 34.0 38.0 21.0 38.0 8 33.42802911134881 38.0 34.0 38.0 21.0 38.0 9 33.699427420877115 38.0 34.0 38.0 21.0 38.0 10-11 33.43839006690577 38.0 34.0 38.0 16.0 38.0 12-13 33.518600082496135 38.0 34.0 38.0 21.0 38.0 14-15 33.434100966882696 38.0 34.0 38.0 16.0 38.0 16-17 33.497909631773595 38.0 34.0 38.0 16.0 38.0 18-19 33.52135811013933 38.0 34.0 38.0 21.0 38.0 20-21 33.53360668917833 38.0 34.0 38.0 16.0 38.0 22-23 33.54984025112383 38.0 34.0 38.0 16.0 38.0 24-25 33.54757859854443 38.0 34.0 38.0 21.0 38.0 26-27 33.490722680145694 38.0 34.0 38.0 21.0 38.0 28-29 33.56145612673644 38.0 34.0 38.0 21.0 38.0 30-31 33.57979054370547 38.0 34.0 38.0 21.0 38.0 32-33 33.64867132280459 38.0 34.0 38.0 21.0 38.0 34-35 33.610331592525014 38.0 34.0 38.0 21.0 38.0 36-37 33.54302033739522 38.0 34.0 38.0 20.0 38.0 38-39 33.53873473297119 38.0 34.0 38.0 16.0 38.0 40-41 33.59522710906968 38.0 34.0 38.0 21.0 38.0 42-43 33.67053629480484 38.0 34.0 38.0 21.0 38.0 44-45 33.80021952361976 38.0 35.0 38.0 21.0 38.0 46-47 33.735481029384005 38.0 34.5 38.0 21.0 38.0 48-49 33.67725483616127 38.0 34.5 38.0 19.5 38.0 50-51 33.61616924292316 38.0 34.0 38.0 16.0 38.0 52-53 33.69417003991974 38.0 34.0 38.0 21.0 38.0 54-55 33.682173144011685 38.0 34.0 38.0 21.0 38.0 56-57 33.6492376098492 38.0 34.0 38.0 21.0 38.0 58-59 33.676220138845196 38.0 34.5 38.0 21.0 38.0 60-61 33.71393415689647 38.0 34.5 38.0 20.5 38.0 62-63 33.58434181365661 38.0 34.0 38.0 19.0 38.0 64-65 33.553300894174235 38.0 34.0 38.0 19.0 38.0 66-67 33.453410655984115 38.0 34.0 38.0 19.0 38.0 68-69 33.40243083957297 38.0 34.0 38.0 19.0 38.0 70-71 33.386263694009244 38.0 34.0 38.0 19.0 38.0 72-73 33.39041996126876 38.0 34.0 38.0 15.0 38.0 74-75 33.39610030971008 38.0 34.0 38.0 15.0 38.0 76 31.709152177408644 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14022746112024365 1101 2 0.42639856153978783 1101 3 0.42983050407898205 1101 4 0.11597301332503207 1101 5 -0.2748635591000692 1101 6 -0.18359535787297432 1101 7 -0.22773228054283123 1101 8 -0.2558106758910128 1101 9 -0.5097400133680807 1101 10-11 0.09763608185973283 1101 12-13 0.13123998108923018 1101 14-15 0.17883989615293672 1101 16-17 0.31340890921359943 1101 18-19 0.12246767728238694 1101 20-21 0.06587104466825622 1101 22-23 0.03855533876732409 1101 24-25 -0.31870942517310397 1101 26-27 -0.05511958379405257 1101 28-29 0.07394704171430533 1101 30-31 0.009894695338687143 1101 32-33 0.12050371228793466 1101 34-35 0.1271876811358439 1101 36-37 0.04603043582469013 1101 38-39 0.09259204403351617 1101 40-41 0.024198970074763793 1101 42-43 -0.10173891609768759 1101 44-45 -0.14386606233139076 1101 46-47 0.360240815778333 1101 48-49 0.04559790552462317 1101 50-51 0.14220949451345888 1101 52-53 0.12383444209188355 1101 54-55 -0.10929390773484471 1101 56-57 0.484067060565863 1101 58-59 0.3059436239593083 1101 60-61 0.1260789075498323 1101 62-63 -0.09568431521146437 1101 64-65 0.11723387962531717 1101 66-67 0.14722286751426594 1101 68-69 0.20096720861252493 1101 70-71 0.2515609745656562 1101 72-73 0.2564676098993246 1101 74-75 -0.22328240990021442 1101 76 -0.869942567129165 1102 1 -0.34233656927660405 1102 2 -0.5503394063746505 1102 3 -0.03721143231279811 1102 4 0.20851452753837307 1102 5 -0.7257501338256525 1102 6 -0.46060070546655396 1102 7 -0.5879067809650067 1102 8 -0.24398967954989104 1102 9 0.15920172600709748 1102 10-11 -0.31578919255624527 1102 12-13 0.3036435836785927 1102 14-15 -0.1889267798954677 1102 16-17 -0.2818767648646414 1102 18-19 -0.5934836314707752 1102 20-21 -0.2786758171386623 1102 22-23 -0.30433799081331614 1102 24-25 -0.5757738777483965 1102 26-27 -0.24377002004511183 1102 28-29 -0.09063218710641507 1102 30-31 -0.08889505979781376 1102 32-33 -0.49521539832282 1102 34-35 -0.6176547055796107 1102 36-37 -0.010977726291834244 1102 38-39 -0.12215605616349734 1102 40-41 -0.4498159468968126 1102 42-43 -0.17137021359275906 1102 44-45 -0.12030146902996108 1102 46-47 -0.6713662768194766 1102 48-49 -0.3223657871294776 1102 50-51 -0.16487606698387225 1102 52-53 -0.3016440268076366 1102 54-55 -0.612755770949029 1102 56-57 -0.335205385254568 1102 58-59 -0.630455193941053 1102 60-61 -0.0986114949269492 1102 62-63 -0.19537471768823877 1102 64-65 -0.23542584455144322 1102 66-67 -0.11844698894505967 1102 68-69 -0.452360964762093 1102 70-71 -0.23955920556381471 1102 72-73 0.05662240866092816 1102 74-75 -0.06607019487066168 1102 76 0.5306766279172734 1103 1 1.0666128690845973 1103 2 0.5160811012223299 1103 3 0.5765027402249032 1103 4 1.1094567225982175 1103 5 0.5261667945618633 1103 6 0.07778030350268494 1103 7 0.6093882986830081 1103 8 0.4438551553537664 1103 9 0.7082354809232072 1103 10-11 0.39823480087950713 1103 12-13 0.4826323509026551 1103 14-15 0.4243912745990457 1103 16-17 0.46452280506434107 1103 18-19 0.7977183038488107 1103 20-21 0.7582338962416344 1103 22-23 0.4849908720449605 1103 24-25 0.5581493885278164 1103 26-27 0.25303274146353516 1103 28-29 0.5284861673060561 1103 30-31 0.5725513369426949 1103 32-33 0.5796933530565198 1103 34-35 0.1258022731714874 1103 36-37 0.7569466151619224 1103 38-39 0.1333188610761269 1103 40-41 0.22985895336631756 1103 42-43 0.33914106162439595 1103 44-45 0.34759174886325894 1103 46-47 0.6148077887663561 1103 48-49 0.4158638481589847 1103 50-51 -0.18834606104210394 1103 52-53 0.3463838712202545 1103 54-55 0.5112574707112714 1103 56-57 0.9131606278173265 1103 58-59 0.47903607731492315 1103 60-61 0.34077537093050125 1103 62-63 0.4926893968464583 1103 64-65 0.6459863162724844 1103 66-67 0.5737127032146319 1103 68-69 0.5507513912914419 1103 70-71 0.27477177935541164 1103 72-73 0.647347587621411 1103 74-75 0.6358863453211754 1103 76 0.9782334284152512 1104 1 0.13563078666648565 1104 2 0.03514529783221576 1104 3 0.1395872719778417 1104 4 0.7143926917639511 1104 5 0.370869572685006 1104 6 0.16966834969883138 1104 7 0.559089211830397 1104 8 0.18619839378055758 1104 9 -0.3311293221491738 1104 10-11 0.20288549782785736 1104 12-13 0.16390454066048932 1104 14-15 0.10187480064654864 1104 16-17 0.1438747605259536 1104 18-19 0.3525796739843301 1104 20-21 0.00498400330505433 1104 22-23 0.03887140307953274 1104 24-25 0.12599306211560446 1104 26-27 0.18385595557656842 1104 28-29 -0.03172837397897865 1104 30-31 0.4935074478465893 1104 32-33 0.44462766446602586 1104 34-35 0.46735876812223154 1104 36-37 0.020339904012132592 1104 38-39 0.421333283555299 1104 40-41 0.23605481726453093 1104 42-43 0.33369464168711716 1104 44-45 0.3367956114352708 1104 46-47 0.11653461816942468 1104 48-49 0.008546235880537267 1104 50-51 0.18052542140640782 1104 52-53 0.03392445171122915 1104 54-55 0.18678277221568607 1104 56-57 0.2391687508228415 1104 58-59 0.23311484836496987 1104 60-61 0.2853924983152609 1104 62-63 0.15272849226414564 1104 64-65 0.3416395412990738 1104 66-67 0.058920682547935144 1104 68-69 -0.12625898349961062 1104 70-71 0.2361287909735168 1104 72-73 0.36252779953791503 1104 74-75 0.17645992897569585 1104 76 0.4736193402502522 1105 1 0.731502404203141 1105 2 0.534922734567779 1105 3 0.7876582796247185 1105 4 0.9086206629835303 1105 5 0.9041909473951755 1105 6 0.8578804690990367 1105 7 0.72441717707067 1105 8 0.23253805854048437 1105 9 0.5702452551467161 1105 10-11 0.8041982889040682 1105 12-13 -0.03700697217178828 1105 14-15 0.4318431102951621 1105 16-17 0.24715740258442764 1105 18-19 0.6451374241686949 1105 20-21 0.8078926062056908 1105 22-23 0.8957001090900789 1105 24-25 0.37100531970435924 1105 26-27 0.6512919603334453 1105 28-29 0.605619734897644 1105 30-31 0.7829293812902094 1105 32-33 0.4774275103326602 1105 34-35 0.6651112113353861 1105 36-37 0.42905574719471673 1105 38-39 0.4931879995712123 1105 40-41 0.8853781585038476 1105 42-43 0.4440826990087814 1105 44-45 0.4508875200979645 1105 46-47 0.4697292314740551 1105 48-49 0.7275484880878977 1105 50-51 0.530059367286988 1105 52-53 0.4379465353020038 1105 54-55 0.37557953541501377 1105 56-57 0.15637521645176378 1105 58-59 0.2231493290617621 1105 60-61 0.3862335881444494 1105 62-63 0.7862897038055436 1105 64-65 0.29561877315684626 1105 66-67 0.8892444290517005 1105 68-69 0.808041669574088 1105 70-71 0.7495789201930805 1105 72-73 0.7281161970159644 1105 74-75 0.4402605122907559 1105 76 0.452020738080428 1106 1 0.568929397307862 1106 2 0.6007508931460066 1106 3 0.9419384942616951 1106 4 0.6869301990354231 1106 5 0.6542337956858582 1106 6 0.5614823623342815 1106 7 0.10570104370042088 1106 8 0.5565700167732928 1106 9 1.007917472315043 1106 10-11 0.4593486534441311 1106 12-13 0.8281857132953903 1106 14-15 0.8693703024796378 1106 16-17 1.022312645237598 1106 18-19 0.757871276955143 1106 20-21 1.0538516827920645 1106 22-23 0.7749338498117737 1106 24-25 0.895389293125227 1106 26-27 0.6198940513300286 1106 28-29 0.9626844292245238 1106 30-31 0.7299655979838988 1106 32-33 0.8375476173874645 1106 34-35 0.6364391128238722 1106 36-37 0.559815546269192 1106 38-39 0.6776070426700045 1106 40-41 0.7114569466223486 1106 42-43 0.4670230146358847 1106 44-45 0.7128356011537491 1106 46-47 0.5294663407306075 1106 48-49 0.7130031414122655 1106 50-51 0.6780903235289983 1106 52-53 0.9492980363105943 1106 54-55 0.3849929752757859 1106 56-57 0.922947616878929 1106 58-59 0.61219532834982 1106 60-61 0.7246131317112727 1106 62-63 0.504792914131329 1106 64-65 0.7439041823250676 1106 66-67 0.688312313928229 1106 68-69 1.1720675631257507 1106 70-71 1.1056515117895458 1106 72-73 0.683260628715793 1106 74-75 0.7034083454856628 1106 76 1.2341206998465637 1107 1 -0.0772215043012281 1107 2 -0.05130592394833755 1107 3 0.5478280044261581 1107 4 -0.3740839232115576 1107 5 -0.48622309249820717 1107 6 -0.8751520861051603 1107 7 -0.1163449732244004 1107 8 0.25404906581152886 1107 9 0.27178338615976116 1107 10-11 0.09252568052658461 1107 12-13 0.32032483041570714 1107 14-15 0.1410014014994232 1107 16-17 0.5675263928964185 1107 18-19 0.7573551929052229 1107 20-21 0.43950317909403225 1107 22-23 0.6180442986340111 1107 24-25 0.4404629120022818 1107 26-27 0.6506608633660633 1107 28-29 0.8263425423392192 1107 30-31 0.6869187197797331 1107 32-33 0.6661154328822931 1107 34-35 0.6489242455019024 1107 36-37 0.5529253115363986 1107 38-39 0.6999729633502838 1107 40-41 0.9282088298164624 1107 42-43 0.7889637640856222 1107 44-45 0.6031933738580264 1107 46-47 0.6230723669517815 1107 48-49 0.897128942880542 1107 50-51 0.7088981488670143 1107 52-53 0.693258827593894 1107 54-55 0.6652026899599193 1107 56-57 0.9130873355276776 1107 58-59 0.34410846217623003 1107 60-61 0.49633651066051243 1107 62-63 0.9477263222000047 1107 64-65 1.0319679443385468 1107 66-67 0.8124117301722364 1107 68-69 0.548221062243968 1107 70-71 0.6160381305162232 1107 72-73 0.7515238520879066 1107 74-75 0.4169099244977872 1107 76 0.2113378105961523 1108 1 -0.11853065464160295 1108 2 0.22180538568677122 1108 3 0.38069388299872386 1108 4 0.07852308930237939 1108 5 0.465570202469209 1108 6 0.829790468898679 1108 7 0.060415253646972644 1108 8 0.21863330808467651 1108 9 0.8834427466816521 1108 10-11 0.5371028590389031 1108 12-13 0.34341017034189036 1108 14-15 0.6403642779736316 1108 16-17 0.2732238007732022 1108 18-19 0.519784295599834 1108 20-21 0.33724485320534825 1108 22-23 0.6432106779025659 1108 24-25 0.5848960451957268 1108 26-27 0.628631624936439 1108 28-29 0.47379508702442763 1108 30-31 0.6185768337555899 1108 32-33 0.6145015020378963 1108 34-35 0.5137017024094988 1108 36-37 0.6494079741587129 1108 38-39 0.5225202109073948 1108 40-41 0.6971130466046702 1108 42-43 0.7169584177882058 1108 44-45 0.6195577531127299 1108 46-47 0.8166367268157657 1108 48-49 0.6237336977611179 1108 50-51 0.26353495383103365 1108 52-53 0.346113071736859 1108 54-55 0.5914037024323093 1108 56-57 0.2856453172311504 1108 58-59 0.537007997491564 1108 60-61 0.35356543883868596 1108 62-63 0.6577208512479871 1108 64-65 0.34772151603918644 1108 66-67 0.6141347337941383 1108 68-69 0.534013900144501 1108 70-71 0.8115692630032854 1108 72-73 0.5645350225253765 1108 74-75 0.7015656354099136 1108 76 -0.05315848092941167 1109 1 0.7152652514184439 1109 2 0.5677823735502336 1109 3 0.3171329638838465 1109 4 0.5864553549493294 1109 5 0.613689416027043 1109 6 0.3707127361218028 1109 7 0.4990369925092537 1109 8 0.13136810657429265 1109 9 0.37122054730252785 1109 10-11 0.6924416335980865 1109 12-13 0.18348678609128655 1109 14-15 0.6619270199676919 1109 16-17 0.2718120973405789 1109 18-19 0.5288198828616117 1109 20-21 0.5363155959943526 1109 22-23 0.4383492098680506 1109 24-25 -0.05938552796271779 1109 26-27 0.21518754826586672 1109 28-29 0.1223186234100524 1109 30-31 0.5274500065933196 1109 32-33 0.3845754451448755 1109 34-35 0.6827634678611432 1109 36-37 0.2566323322105575 1109 38-39 0.6615421608398933 1109 40-41 0.41621147522798196 1109 42-43 0.8519714040892055 1109 44-45 0.3890149325840966 1109 46-47 0.7979967591615988 1109 48-49 0.1847864447578189 1109 50-51 0.42334526474844125 1109 52-53 0.5234805874814583 1109 54-55 0.5235973936256073 1109 56-57 0.6738945993868128 1109 58-59 0.20569970476183386 1109 60-61 0.6091522894379722 1109 62-63 0.4059921998359428 1109 64-65 0.5466003768082146 1109 66-67 0.4766752517385342 1109 68-69 0.4063780922184037 1109 70-71 0.6395230468481898 1109 72-73 0.21109176820631603 1109 74-75 0.47541554255271734 1109 76 0.4605645099630422 1110 1 0.04157433223503659 1110 2 -0.15089802638672012 1110 3 -0.07074968655508229 1110 4 -0.07108320991022055 1110 5 0.04299802399151886 1110 6 0.07913430091967655 1110 7 0.015795831074797206 1110 8 -0.2354349333851431 1110 9 -0.2477908491188714 1110 10-11 0.02135482754284368 1110 12-13 -0.04241660131165048 1110 14-15 -0.4318141997166549 1110 16-17 -0.3698208186493659 1110 18-19 -0.8529191164736574 1110 20-21 -0.6050123646424197 1110 22-23 -0.213744702674866 1110 24-25 0.011405231547435335 1110 26-27 -0.5340980338562176 1110 28-29 -0.31229415182072273 1110 30-31 -0.7095071557457189 1110 32-33 -0.16896306489123702 1110 34-35 -0.3366919983779084 1110 36-37 -0.265290188570944 1110 38-39 -0.5549076106149116 1110 40-41 -0.06272947308037402 1110 42-43 -0.4182121858863326 1110 44-45 -0.506426498855781 1110 46-47 -0.4681401760713726 1110 48-49 -0.1728542287480721 1110 50-51 -0.3742603217595146 1110 52-53 -0.28118351618964965 1110 54-55 -0.3219821243393497 1110 56-57 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0.7638355199660225 2116 20-21 0.3658855038932387 2116 22-23 0.7158109778650683 2116 24-25 0.26541434579277023 2116 26-27 0.34969533812613207 2116 28-29 0.07127208509197658 2116 30-31 0.6444377838291402 2116 32-33 0.3734967718599407 2116 34-35 0.1486451710143868 2116 36-37 0.33946594768125493 2116 38-39 0.5518170295742877 2116 40-41 -0.228779630272264 2116 42-43 0.1639782614615939 2116 44-45 0.04753069880221261 2116 46-47 0.2415884155469783 2116 48-49 0.42934573664087594 2116 50-51 0.5718798241837888 2116 52-53 0.12517915001553348 2116 54-55 0.517209851663651 2116 56-57 -0.17230945765275862 2116 58-59 0.2865248848037325 2116 60-61 0.09652425167938361 2116 62-63 0.09179399595105764 2116 64-65 0.36022115550731826 2116 66-67 0.34970985421178113 2116 68-69 0.42417426471430986 2116 70-71 0.3441144736981059 2116 72-73 0.021635336909156422 2116 74-75 0.11360307303790051 2116 76 0.5280909782728003 2117 1 -0.6790470765753476 2117 2 -0.34792576278453424 2117 3 -0.5804113166891547 2117 4 -0.27677236363086877 2117 5 -0.2398706714756038 2117 6 -0.07548724976486199 2117 7 0.06999175928647361 2117 8 -0.16292305142444263 2117 9 -0.1233246506369241 2117 10-11 0.14664034928505743 2117 12-13 -0.5535395352692305 2117 14-15 -0.03172198151420247 2117 16-17 -0.04306335252181981 2117 18-19 0.0784776346081415 2117 20-21 -0.7407079027001657 2117 22-23 0.09467198172607283 2117 24-25 -0.18961980924138544 2117 26-27 -0.23374689531609505 2117 28-29 -0.5177314789115854 2117 30-31 -0.09977813538677793 2117 32-33 -0.3136356902725268 2117 34-35 -0.15015940279018736 2117 36-37 -0.2603707021553987 2117 38-39 -0.4263979986407307 2117 40-41 0.005813479320849524 2117 42-43 -0.28481146232812193 2117 44-45 0.08817518944669445 2117 46-47 0.04843427239283926 2117 48-49 0.043713126008263714 2117 50-51 -0.08954726224330756 2117 52-53 -0.15692658209019328 2117 54-55 -0.1272882178344119 2117 56-57 -0.4146171499604492 2117 58-59 0.07255102082986298 2117 60-61 -0.35695807680293967 2117 62-63 -0.10101859686153603 2117 64-65 -0.31427394398777153 2117 66-67 -0.03638310688118196 2117 68-69 -0.4352703447302986 2117 70-71 0.14309724768087762 2117 72-73 -1.0847130444392263 2117 74-75 -0.266987957553134 2117 76 -0.7698003196184935 2118 1 0.17500697747870575 2118 2 -0.4019798168385904 2118 3 0.2952643589865218 2118 4 -0.006502093360598593 2118 5 0.024994193389261454 2118 6 0.29208031780270005 2118 7 0.11593770523241886 2118 8 -0.025085213586599764 2118 9 -0.1152165425288203 2118 10-11 -0.7466028939581904 2118 12-13 -0.023809805539507067 2118 14-15 0.08044018064795466 2118 16-17 0.0501798907214237 2118 18-19 -0.1715223653918656 2118 20-21 -0.03395114594340498 2118 22-23 -0.08776045070636229 2118 24-25 0.3252450556234763 2118 26-27 -0.14320635477555754 2118 28-29 -0.3880017491818535 2118 30-31 -0.5065348921435344 2118 32-33 -0.2298519064887401 2118 34-35 -0.08799724062802738 2118 36-37 0.046386054601356363 2118 38-39 -0.1872088094515476 2118 40-41 -0.032024358516991924 2118 42-43 0.10437772686105973 2118 44-45 -0.2010139997424929 2118 46-47 -0.22453870058013337 2118 48-49 -0.26574633345119736 2118 50-51 0.06991219721615494 2118 52-53 -0.1623319874956053 2118 54-55 -0.21512605567225052 2118 56-57 -0.21731985266315235 2118 58-59 -0.2382597899809511 2118 60-61 -0.6204715903164555 2118 62-63 -0.11047805632099994 2118 64-65 -0.06021988993371963 2118 66-67 -0.12557229607036646 2118 68-69 -0.3568919663519097 2118 70-71 -0.1987946442110129 2118 72-73 -0.17660493633111685 2118 74-75 -0.5332041737693416 2118 76 0.051821302003126846 2119 1 0.16333299983614324 2119 2 -0.1885229044287975 2119 3 -0.10535825315441372 2119 4 0.07268285074581016 2119 5 0.22306975586273126 2119 6 0.0027920766819988785 2119 7 -0.16079924113803656 2119 8 0.3188655434531569 2119 9 -0.11286759672437796 2119 10-11 0.3486143127773431 2119 12-13 0.524364808865478 2119 14-15 0.16229952690521543 2119 16-17 0.10497683426182647 2119 18-19 0.39931391591562715 2119 20-21 0.15562717583387098 2119 22-23 -0.33252484861211684 2119 24-25 0.09502714137415325 2119 26-27 0.15697052367357145 2119 28-29 0.4317591111549177 2119 30-31 0.07616639553157967 2119 32-33 -0.009270330148005712 2119 34-35 -0.13453042302508322 2119 36-37 0.06899819553810715 2119 38-39 -0.2107973194274919 2119 40-41 -0.14920279349647103 2119 42-43 -0.05109135478603122 2119 44-45 -0.10007300639311723 2119 46-47 -0.16260294283285504 2119 48-49 -0.17812074975231695 2119 50-51 -0.03462296621953698 2119 52-53 -0.03355740139117103 2119 54-55 -0.34769291572602157 2119 56-57 -0.4235176735206636 2119 58-59 -0.2776400078951937 2119 60-61 -0.27485110071690144 2119 62-63 -0.2904624910174718 2119 64-65 0.059986704094860954 2119 66-67 -0.3690419437284973 2119 68-69 -0.8899187103734221 2119 70-71 -0.393134533838861 2119 72-73 -0.24288482878901618 2119 74-75 0.13596945011632044 2119 76 0.5420717618870938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 16.0 13 333.0 14 1136.0 15 2032.0 16 2534.0 17 3076.0 18 3213.0 19 2787.0 20 2188.0 21 1590.0 22 1175.0 23 1025.0 24 900.0 25 948.0 26 1031.0 27 1278.0 28 1402.0 29 1826.0 30 2257.0 31 2804.0 32 3583.0 33 4770.0 34 6640.0 35 9853.0 36 18545.0 37 66093.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.59814593426876 18.24003579493418 13.244824765620084 28.91699350517698 2 20.535945244936624 20.3220145836392 31.138796255514308 28.00324391590987 3 19.7641169767263 21.880352636031237 25.859043464278475 32.49648692296399 4 16.555856177073064 17.656270755119305 27.290840831393275 38.497032236414356 5 17.884183812579963 30.06354999056188 30.46274740102211 21.589518795836042 6 32.38672511308263 28.909303187287204 17.656969874927466 21.0470018247027 7 30.51588050644239 25.51787299789565 18.651118242133155 25.31512825352881 8 25.46264253305089 29.17426959458042 20.101092724260155 25.26199514810853 9 24.441228493326904 16.141278130833282 19.205520250005243 40.211973125834575 10-11 28.329033746513137 21.40355292686508 22.89162943853688 27.375783888084904 12-13 26.68994735627844 18.885323377867266 22.95000594251837 31.47472332333592 14-15 25.99047798821284 19.932255290589147 20.632773338367 33.44449338283101 16-17 27.061879094220377 21.605598551423757 21.530792731950473 29.801729622405393 18-19 26.502932807595236 20.861035955731733 22.063871585673635 30.572159650999392 20-21 26.21000160797556 21.183679747198276 22.315205156707705 30.29111348811846 22-23 27.72010039360445 20.974992484462064 21.324552388542823 29.980354733390662 24-25 26.926599411341122 21.81638317358446 20.701636639470905 30.555380775603513 26-27 25.978592951474095 21.993959604857487 22.06037598663283 29.967071457035594 28-29 26.496640729321786 21.63006774470941 23.08913078434251 28.78416074162629 30-31 26.634017771625523 21.059236421345524 22.79410222529835 29.5126435817306 32-33 25.524165076169105 22.109663933108216 23.209379391346292 29.156791599376387 34-35 25.67762187406056 21.253941287918508 23.774967316148967 29.293469521871963 36-37 25.67902011367688 20.385634486181896 24.190244482196917 29.745100917944306 38-39 24.325873724980248 20.352775855198306 26.54033571733188 28.781014702489564 40-41 24.025601767374873 20.156323189104917 26.974489118200186 28.84358592532002 42-43 22.516202101554146 20.08955724742549 27.261128239546412 30.133112411473956 44-45 21.045327591209237 20.274545308365347 29.041188787477324 29.638938312948092 46-47 21.00959891496606 21.397260848591625 29.19454406901711 28.39859616742521 48-49 20.622286541244573 22.03415899382677 28.7586428686284 28.584911596300255 50-51 19.943510922037383 23.21715086708637 27.63490437755398 29.204433833322263 52-53 19.069891007221905 23.51908946636185 27.96619056607731 29.44482896033893 54-55 19.23453372204395 22.832903374651316 27.869362472646937 30.0632004306578 56-57 19.803617245887427 23.218817508756477 27.339779217964583 29.637786027391517 58-59 20.115424680327468 23.19924215412795 26.512720484909497 30.17261268063508 60-61 20.173451624404876 22.39001097618099 26.774540853065993 30.661996546348146 62-63 20.312226906324938 22.41028545061767 26.212098967400046 31.065388675657346 64-65 20.808252410215538 23.109754818683278 25.040374168921325 31.041618602179856 66-67 20.907876982878555 24.121730740997087 23.586904087753517 31.38348818837084 68-69 20.902633584317346 25.23787551472696 23.34465907422555 30.51483182673015 70-71 21.172144270363614 25.82059187482959 23.116396456860812 29.890867397945986 72-73 21.560155763893256 26.032425176702535 22.947209463285724 29.460209596118485 74-75 21.50562441885666 26.854939631004566 23.83369338003454 27.805742570104236 76 21.72095331977041 26.399463075987335 24.190244482196917 27.689339122045343 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 2.0 15 5.5 16 7.0 17 7.0 18 8.0 19 17.5 20 33.0 21 40.0 22 46.0 23 73.0 24 115.0 25 136.0 26 198.0 27 334.0 28 465.5 29 523.0 30 609.0 31 790.0 32 1141.5 33 1398.0 34 1597.5 35 2017.5 36 2411.5 37 2585.0 38 2906.0 39 3705.5 40 4555.0 41 4955.0 42 4984.0 43 5218.5 44 5658.0 45 5933.0 46 6003.0 47 5958.0 48 5772.0 49 5269.5 50 4908.0 51 4653.5 52 4132.5 53 3836.5 54 3807.0 55 3765.0 56 3952.5 57 4507.0 58 4832.0 59 5232.5 60 5922.0 61 6483.5 62 6756.0 63 6928.5 64 6925.0 65 6641.5 66 6194.0 67 5854.0 68 5146.5 69 3742.0 70 2462.5 71 1880.0 72 1459.0 73 703.5 74 255.5 75 142.0 76 95.0 77 37.5 78 19.5 79 12.0 80 13.0 81 13.0 82 11.5 83 11.0 84 8.0 85 5.0 86 4.5 87 4.0 88 4.0 89 6.0 90 5.5 91 6.0 92 9.0 93 5.0 94 2.0 95 3.5 96 4.0 97 4.5 98 6.0 99 22.5 100 38.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.495599040807623E-4 46-47 0.0 48-49 0.0 50-51 3.495599040807623E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 143037.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.24789390157791 #Duplication Level Percentage of deduplicated Percentage of total 1 75.3775366983611 49.93603053755322 2 14.635021475532668 19.390786999168046 3 5.005329309089374 9.947775750330335 4 1.8963897888327232 5.025273181065039 5 0.9772158845070125 3.2369247117878595 6 0.5698667145073291 2.2651481784433396 7 0.4463955930307411 2.0700937519662745 8 0.28493335725366453 1.5100987856288932 9 0.21211705484439472 1.264707732964198 >10 0.594138815310419 5.3175052608765565 >50 0.001055308730569128 0.035655110216237754 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003495599040807623 0.0 2 0.0 0.0 0.0 0.0055929584652921974 0.0 3 0.0 0.0 0.0 0.006292078273453722 0.0 4 0.0 0.0 0.0 0.01817711501219964 0.0 5 0.0 0.0 0.0 0.026566552710137935 0.0 6 0.0 0.0 0.0 0.027964792326460985 0.0 7 0.0 0.0 0.0 0.027964792326460985 0.0 8 0.0 0.0 0.0 0.02866391213462251 0.0 9 0.0 0.0 0.0 0.037053349832560804 0.0 10 0.0 0.0 0.0 0.042646308297853 0.0 11 0.0 0.0 0.0 0.04404454791417605 0.0 12 0.0 0.0 0.0 0.04404454791417605 0.0 13 0.0 0.0 0.0 0.0454427875304991 0.0 14 0.0 0.0 0.0 0.0482392667631452 0.0 15 0.0 0.0 0.0 0.051734865803952826 0.0 16 0.0 0.0 0.0 0.05453134503659892 0.0 17 0.0 0.0 0.0 0.05592958465292197 0.0 18 0.0 0.0 0.0 0.05802694407740654 0.0 19 0.0 0.0 0.0 0.06851374119982942 0.0 20 0.0 0.0 0.0 0.07900053832225229 0.0 21 0.0 0.0 0.0 0.08109789774673686 0.0 22 0.0 0.0 0.0 0.09158469486915972 0.0 23 0.0 0.0 0.0 0.09787677314261345 0.0 24 0.0 0.0 0.0 0.10626621084055174 0.0 25 0.0 0.0 0.0 0.11465564853849004 0.0 26 0.0 0.0 0.0 0.12933716450988206 0.0 27 0.0 0.0 0.0 0.28244440249725594 0.0 28 0.0 0.0 0.0 0.48728650628858267 0.0 29 0.0 0.0 0.0 0.7361731579940854 0.0 30 0.0 0.0 0.0 0.9696791739200347 0.0 31 0.0 0.0 0.0 1.2437341387193523 0.0 32 0.0 0.0 0.0 1.5667274900899768 0.0 33 0.0 0.0 0.0 1.9764116976726303 0.0 34 0.0 0.0 0.0 2.5168313093814887 0.0 35 0.0 0.0 0.0 3.1768004082859678 0.0 36 0.0 0.0 0.0 4.18632941127121 0.0 37 0.0 0.0 0.0 5.497878171382229 0.0 38 0.0 0.0 0.0 6.926179939456224 0.0 39 0.0 0.0 0.0 8.310437159616043 0.0 40 0.0 0.0 0.0 9.645755993204556 0.0 41 0.0 0.0 0.0 11.085942798017296 0.0 42 0.0 0.0 0.0 12.452022903164915 0.0 43 0.0 0.0 0.0 13.850262519487964 0.0 44 0.0 0.0 0.0 15.201661108664192 0.0 45 0.0 0.0 0.0 16.581723609975043 0.0 46 0.0 0.0 0.0 17.910051245481938 0.0 47 0.0 0.0 0.0 19.22579472444193 0.0 48 0.0 0.0 0.0 20.38703272579822 0.0 49 0.0 0.0 0.0 21.549668966770835 0.0 50 0.0 0.0 0.0 22.63819850807833 6.991198081615247E-4 51 0.0 0.0 0.0 23.686878220320615 6.991198081615247E-4 52 0.0 0.0 0.0 24.787991918175017 6.991198081615247E-4 53 0.0 0.0 0.0 25.854149625621343 6.991198081615247E-4 54 0.0 0.0 0.0 26.858085670141293 6.991198081615247E-4 55 0.0 0.0 0.0 27.871809391975503 6.991198081615247E-4 56 0.0 0.0 0.0 28.806532575487463 6.991198081615247E-4 57 0.0 0.0 0.0 29.706998888399504 6.991198081615247E-4 58 0.0 0.0 0.0 30.62564231632375 6.991198081615247E-4 59 0.0 0.0 0.0 31.475772003048164 6.991198081615247E-4 60 0.0 0.0 0.0 32.334990247278675 6.991198081615247E-4 61 0.0 0.0 0.0 33.16904017841537 6.991198081615247E-4 62 0.0 0.0 0.0 34.00099275012759 6.991198081615247E-4 63 0.0 0.0 0.0 34.81336996721128 6.991198081615247E-4 64 0.0 0.0 0.0 35.6180568664052 6.991198081615247E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTGAG 15 0.002220912 70.0 54 TTGGCAA 20 0.006939658 52.5 28 ATAGACA 20 0.006939658 52.5 27 ATGTAGG 20 0.006939658 52.5 1 GTAGCAA 20 0.006939658 52.5 35 CGATATG 20 0.006939658 52.5 65 ACGAATT 20 0.006939658 52.5 24 GTTTATC 40 0.0024123802 35.0 38 GTAATAC 40 0.0024123802 35.0 3 TGCTTAC 45 0.004295979 31.111109 7 GCCGTAT 45 0.004295979 31.111109 54 TTTTAGT 45 0.004295979 31.111109 36 CCGTATC 60 4.962758E-4 29.166666 55 TCTACGA 50 0.007189605 28.000002 2 TGTATAT 75 0.0018252302 23.333334 38 AGAGCAC 75 0.0018252302 23.333334 18 TAGATCT 120 2.1469295E-6 23.333332 39 AGCACAC 95 2.7649442E-4 22.105263 20 AGATCTC 135 5.9641206E-6 20.740742 40 GTATTAG 85 0.0037747945 20.588236 1 >>END_MODULE