Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780296_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 143037 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT | 9311 | 6.509504533791956 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC | 3001 | 2.0980585442927353 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG | 2304 | 1.6107720380041528 | No Hit |
CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG | 2219 | 1.5513468543104232 | Illumina PCR Primer Index 8 (95% over 23bp) |
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC | 635 | 0.44394107818256817 | No Hit |
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT | 330 | 0.23070953669330313 | RNA PCR Primer, Index 15 (95% over 21bp) |
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT | 214 | 0.14961163894656626 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTGCAAA | 20 | 7.904938E-5 | 70.0 | 10 |
TTCGTTC | 15 | 0.002220912 | 70.0 | 22 |
CAAAGGT | 20 | 7.904938E-5 | 70.0 | 13 |
CTTTATA | 95 | 0.0 | 58.947365 | 2 |
TAACCGT | 25 | 2.3839394E-4 | 56.000004 | 5 |
CACTTGT | 25 | 2.3839394E-4 | 56.000004 | 28 |
TCTTTAT | 100 | 0.0 | 52.500004 | 1 |
CTTCCGG | 20 | 0.006939658 | 52.5 | 15 |
AGACCCG | 20 | 0.006939658 | 52.5 | 5 |
AACCGTG | 30 | 5.862055E-4 | 46.666664 | 6 |
AAACAAG | 30 | 5.862055E-4 | 46.666664 | 18 |
CTTAATA | 70 | 1.9081199E-8 | 40.0 | 1 |
ATAGGGA | 35 | 0.001252086 | 40.0 | 42 |
CATGACT | 35 | 0.001252086 | 40.0 | 29 |
ACCTGAA | 35 | 0.001252086 | 40.0 | 13 |
TGAAACA | 35 | 0.001252086 | 40.0 | 16 |
CTGAAAC | 35 | 0.001252086 | 40.0 | 15 |
GAAACAA | 35 | 0.001252086 | 40.0 | 17 |
GTTCCTT | 35 | 0.001252086 | 40.0 | 33 |
TCACTTG | 35 | 0.001252086 | 40.0 | 27 |