FastQCFastQC Report
Wed 25 May 2016
SRR1780296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143037
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT93116.509504533791956No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC30012.0980585442927353No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG23041.6107720380041528No Hit
CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG22191.5513468543104232Illumina PCR Primer Index 8 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC6350.44394107818256817No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT3300.23070953669330313RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT2140.14961163894656626No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAA207.904938E-570.010
TTCGTTC150.00222091270.022
CAAAGGT207.904938E-570.013
CTTTATA950.058.9473652
TAACCGT252.3839394E-456.0000045
CACTTGT252.3839394E-456.00000428
TCTTTAT1000.052.5000041
CTTCCGG200.00693965852.515
AGACCCG200.00693965852.55
AACCGTG305.862055E-446.6666646
AAACAAG305.862055E-446.66666418
CTTAATA701.9081199E-840.01
ATAGGGA350.00125208640.042
CATGACT350.00125208640.029
ACCTGAA350.00125208640.013
TGAAACA350.00125208640.016
CTGAAAC350.00125208640.015
GAAACAA350.00125208640.017
GTTCCTT350.00125208640.033
TCACTTG350.00125208640.027