FastQCFastQC Report
Wed 25 May 2016
SRR1780295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386931
Sequences flagged as poor quality0
Sequence length50
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGA407.069891E-427.4751661
TTAATAC407.0728717E-427.4731853
TATACAG407.0728717E-427.4731853
CTTAATA508.391661E-526.377112
CGCAAAG703.2068834E-522.00790612
TATCCAC602.8920898E-421.9785483
GTTATAC654.962806E-420.2893541
TAAACGG550.00448678919.99780326
TACAGTA550.004489600719.99563810
ACGCTAA550.004509322319.98056
TAATACA1203.195364E-718.3154564
TAACACG600.007454463718.3154564
TACGCAA1154.282687E-617.21363610
ACGCAAA1307.3354204E-716.91938611
CAAAGGC1053.7540653E-516.77277414
GGATTGT1053.8066908E-516.745566
TCGTTAC800.001982342516.50771713
GAGTATA1206.463175E-616.48511
GTAATAC800.002001557316.4839123
ATAACAA953.3495593E-416.20874430
Produced by FastQC (version 0.11.5)