FastQCFastQC Report
Wed 25 May 2016
SRR1780295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386931
Sequences flagged as poor quality0
Sequence length50
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT329812.377984196762492No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC110390.7959300066117204No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG85660.6176226502976717No Hit
CACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTG65370.4713284222502777Illumina PCR Primer Index 1 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC18730.1350463721699205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT207.86104E-443.9954625
TCTTTAT2750.038.4862251
CTTTATA2900.036.4192282
CTCGCTA250.002354103835.19636513
TCTTCTG9900.034.22485744
GTCTTCT10400.032.79098543
CCTTATA651.7096681E-830.5299361
ATGCCGT49250.030.51199744
TTTATAC3800.030.1097563
CCGTCTT11650.029.46149841
CGTCTTC13000.026.74052242
TATGCCG58300.025.73783343
GCTATAA551.592951E-424.00184843
TTAATAC1205.984475E-1022.003283
ACTTGAA904.0768464E-722.003282
GCTGCAA500.002579473422.00169642
GCCGTCT15500.022.000940
TAAAGTC500.00258217521.99772828
AACGATT500.00258217521.99772822
TGCAAAA500.00258217521.99772810
Produced by FastQC (version 0.11.5)