FastQCFastQC Report
Wed 25 May 2016
SRR1780294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89557
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAA150.00221819370.023
CCGTAAA252.3791096E-455.99999616
GACGCTT404.5607845E-543.75000414
CGTTGCT350.001249579140.00000428
TCACATA400.0024075835.00000451
TGGTGTA400.0024075835.00000434
CTCTAAT400.0024075835.00000441
TCCACAT957.477865E-933.1578985
ATCCACA1001.2330929E-831.4999984
AATGATT450.004287482731.11111325
GATCTCT450.004287482731.11111341
TACAACG450.004287482731.11111322
TATCCAC901.7281855E-731.1111133
ACGCTTC703.455401E-530.00000215
TTATCCA1202.242814E-929.1666662
TGTAGAT853.905663E-628.8235337
CGACGAT500.007175474427.99999822
CTTATAA500.007175474427.9999981
TCTAATA500.007175474427.99999842
TAGATCT1109.994837E-725.45454839
Produced by FastQC (version 0.11.5)