##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780294_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 89557 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.342318300077046 34.0 27.0 34.0 12.0 34.0 2 29.40828745938341 34.0 27.0 34.0 12.0 34.0 3 29.152763044764786 34.0 27.0 34.0 11.0 34.0 4 29.06259700525922 34.0 27.0 34.0 11.0 34.0 5 28.98315039583729 34.0 27.0 34.0 11.0 34.0 6 31.748149223399622 37.0 28.0 38.0 11.0 38.0 7 32.15510792009558 37.0 31.0 38.0 12.0 38.0 8 32.174615049633196 37.0 31.0 38.0 11.0 38.0 9 32.350625858391865 38.0 31.0 38.0 12.0 38.0 10-11 32.034944225465345 37.5 31.0 38.0 11.0 38.0 12-13 32.11674129325458 37.0 31.0 38.0 11.0 38.0 14-15 31.930669852719497 37.5 31.0 38.0 11.0 38.0 16-17 31.99950310975133 38.0 31.0 38.0 11.0 38.0 18-19 32.013466284042565 38.0 31.0 38.0 11.0 38.0 20-21 32.0324541911855 38.0 31.0 38.0 11.0 38.0 22-23 32.01729624708286 38.0 31.0 38.0 11.0 38.0 24-25 32.01636387998705 38.0 31.0 38.0 11.0 38.0 26-27 31.939329142333932 38.0 31.0 38.0 11.0 38.0 28-29 31.95999754346394 38.0 31.0 38.0 11.0 38.0 30-31 31.997264312114073 38.0 31.0 38.0 11.0 38.0 32-33 32.05155375905848 38.0 31.0 38.0 11.0 38.0 34-35 32.02336500776042 38.0 31.0 38.0 11.0 38.0 36-37 31.960935493596256 38.0 30.5 38.0 11.0 38.0 38-39 31.892308808914994 38.0 29.0 38.0 11.0 38.0 40-41 31.99766070770571 38.0 31.0 38.0 11.0 38.0 42-43 32.08779883202877 38.0 31.0 38.0 11.0 38.0 44-45 32.19330147280503 38.0 31.0 38.0 11.0 38.0 46-47 32.078838058443225 38.0 31.0 38.0 11.0 38.0 48-49 31.976657324385588 38.0 31.0 38.0 11.0 38.0 50-51 31.921128443337764 38.0 29.5 38.0 11.0 38.0 52-53 31.98905724845629 38.0 30.0 38.0 11.0 38.0 54-55 31.981274495572656 38.0 30.5 38.0 11.0 38.0 56-57 31.969980012729323 38.0 31.0 38.0 11.0 38.0 58-59 31.969008564377994 38.0 30.5 38.0 11.0 38.0 60-61 32.015900487957396 38.0 31.0 38.0 11.0 38.0 62-63 31.88209185211653 38.0 29.5 38.0 11.0 38.0 64-65 31.8646057817926 38.0 29.0 38.0 11.0 38.0 66-67 31.81527407126188 37.5 28.0 38.0 11.0 38.0 68-69 31.719363087195866 37.0 28.0 38.0 11.0 38.0 70-71 31.731528523733488 37.0 27.5 38.0 11.0 38.0 72-73 31.68933193385218 37.0 28.0 38.0 11.0 38.0 74-75 31.69041504293355 37.0 27.5 38.0 11.0 38.0 76 30.147180008262893 36.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47633247296313286 1101 2 0.40776298963168855 1101 3 0.5054743687430197 1101 4 0.6670150748700046 1101 5 -0.8467828423206214 1101 6 0.37379941916247716 1101 7 -0.1999778282309812 1101 8 -0.7340844264478754 1101 9 -0.30586690052746235 1101 10-11 -0.2171547176244708 1101 12-13 -0.23516043831528322 1101 14-15 0.0551111792780965 1101 16-17 -0.4147391673655285 1101 18-19 -0.14115107298516705 1101 20-21 0.1166123750089767 1101 22-23 -0.3043109123869172 1101 24-25 -0.1930344538448523 1101 26-27 -0.3227799741828328 1101 28-29 0.15744187271026533 1101 30-31 0.5692499623353058 1101 32-33 -0.0812226557553899 1101 34-35 -0.1300107693941399 1101 36-37 0.1099337676583545 1101 38-39 -0.18909482147430268 1101 40-41 0.05303259059008525 1101 42-43 -0.17843069415028623 1101 44-45 0.5817230445044501 1101 46-47 0.397021452661793 1101 48-49 0.21429486662073316 1101 50-51 0.3431184027719887 1101 52-53 0.8853748545567939 1101 54-55 -0.14169089643002053 1101 56-57 -0.09089853039573725 1101 58-59 0.1788858033583267 1101 60-61 0.13979888855983091 1101 62-63 0.153903877450567 1101 64-65 -0.027680936395267253 1101 66-67 -0.2922457004436474 1101 68-69 0.14173680733057736 1101 70-71 0.01166546159458548 1101 72-73 -0.18835404922614885 1101 74-75 0.24400491824383863 1101 76 -0.445265398096339 1102 1 0.6030013387420539 1102 2 0.34297019809017115 1102 3 0.8746127218394264 1102 4 1.3504644403103754 1102 5 0.6755873377172286 1102 6 1.202667975908252 1102 7 0.6261302639233932 1102 8 0.8596758818908157 1102 9 0.36563781309603627 1102 10-11 0.5806716335274515 1102 12-13 0.8629749459795804 1102 14-15 0.21771816898278118 1102 16-17 0.7711668379470638 1102 18-19 0.30446430228176524 1102 20-21 0.8601582699029819 1102 22-23 0.7811593214713142 1102 24-25 0.5988456135174332 1102 26-27 0.7591485315203919 1102 28-29 0.2875569788753012 1102 30-31 0.33608619213096347 1102 32-33 0.45001920608248724 1102 34-35 0.06316098705996609 1102 36-37 0.29760195634219144 1102 38-39 0.937782381254916 1102 40-41 0.052164519963859846 1102 42-43 0.4898274353311294 1102 44-45 0.5317829413776636 1102 46-47 0.08668620378594838 1102 48-49 0.5669659199376298 1102 50-51 0.4400124460713535 1102 52-53 0.4251411699442116 1102 54-55 0.2797313504839849 1102 56-57 0.6771877211016815 1102 58-59 -0.259897856080201 1102 60-61 0.4951870029368095 1102 62-63 0.5101427010412536 1102 64-65 0.5602546180414407 1102 66-67 0.19112206635786322 1102 68-69 0.340290601667288 1102 70-71 0.16973244192410775 1102 72-73 0.39841829057141354 1102 74-75 0.630209539851851 1102 76 0.2537050701409562 1103 1 0.7809144058803419 1103 2 1.3338381662555996 1103 3 1.0112360807945997 1103 4 0.5841390202709249 1103 5 0.8160369669941474 1103 6 1.4163949332580934 1103 7 1.2599817026010385 1103 8 1.359114695576956 1103 9 1.3686478124158157 1103 10-11 0.8758386289699445 1103 12-13 1.0061369757734724 1103 14-15 0.9402370452496989 1103 16-17 1.4787377514903568 1103 18-19 0.8854196794669917 1103 20-21 1.0438107425238954 1103 22-23 1.0091965298244858 1103 24-25 1.0440874338017636 1103 26-27 0.9225557827277875 1103 28-29 0.9797547885481137 1103 30-31 1.1021140133676113 1103 32-33 0.841208580954941 1103 34-35 0.3377596538173293 1103 36-37 0.7895242745518303 1103 38-39 0.27173925493697126 1103 40-41 0.6000694243921245 1103 42-43 0.5823296460650909 1103 44-45 0.7073832406770322 1103 46-47 0.686050192630244 1103 48-49 1.0882059716347996 1103 50-51 0.5680309482140622 1103 52-53 0.7571338915277792 1103 54-55 0.7052636271998551 1103 56-57 0.9901341193058855 1103 58-59 0.8218482836381824 1103 60-61 1.0070994578718526 1103 62-63 0.41469851357101817 1103 64-65 0.7550287830642262 1103 66-67 0.6953734778264646 1103 68-69 1.0264854183464216 1103 70-71 1.1099660994984184 1103 72-73 0.8558276504789006 1103 74-75 0.5931372318455921 1103 76 1.52247930318633 1104 1 -0.38816837423075157 1104 2 0.11262830309342675 1104 3 -9.573889249523404E-4 1104 4 -0.11783225733373826 1104 5 -0.5168786270987944 1104 6 0.021055548350897624 1104 7 0.2964604995120226 1104 8 -0.1570416518014497 1104 9 0.48235191755061635 1104 10-11 0.36100324081115787 1104 12-13 0.21618642706734192 1104 14-15 -0.011903586101066566 1104 16-17 0.3113146380910905 1104 18-19 0.21046032436606055 1104 20-21 -0.19106596794894415 1104 22-23 0.02997623447155462 1104 24-25 -0.021486336690038854 1104 26-27 0.057869425865579416 1104 28-29 0.2061468763410943 1104 30-31 -0.055909967122829585 1104 32-33 0.3363142576925142 1104 34-35 -0.09041742245960194 1104 36-37 -0.226462719080466 1104 38-39 0.06241802475136282 1104 40-41 -0.1507434735891735 1104 42-43 -0.08514427345800613 1104 44-45 -0.5703569029349467 1104 46-47 -0.7442438054729941 1104 48-49 -0.20826469629637856 1104 50-51 -0.6732981399853699 1104 52-53 -0.3827014966310074 1104 54-55 -0.08964898626715723 1104 56-57 -0.27410853074152897 1104 58-59 -0.2854542498938599 1104 60-61 -0.2218837422365354 1104 62-63 -0.29741705342532754 1104 64-65 0.42392527405461067 1104 66-67 0.31307433136935003 1104 68-69 -0.1722003965838077 1104 70-71 -0.1375444004338391 1104 72-73 -0.009146607751688407 1104 74-75 -0.15222247551113455 1104 76 -0.14081157880798045 1105 1 -0.2936826460551849 1105 2 0.11703833156762755 1105 3 0.667318185767737 1105 4 0.5142602490903201 1105 5 0.031596961483746355 1105 6 0.8911159692134554 1105 7 0.11915652006900501 1105 8 0.4442848292041788 1105 9 0.18608134320518133 1105 10-11 0.2822651339981874 1105 12-13 -0.5190903282628909 1105 14-15 -0.14486420836650282 1105 16-17 0.22643027112115988 1105 18-19 0.7697873609658217 1105 20-21 0.4806994324260856 1105 22-23 0.690230418789902 1105 24-25 -0.3261081854295327 1105 26-27 0.41094950087078885 1105 28-29 0.0022785521519139706 1105 30-31 0.5717551485101922 1105 32-33 0.13930404865021728 1105 34-35 0.8509673959305672 1105 36-37 0.1021017645642246 1105 38-39 0.6332215527741418 1105 40-41 0.32658134063402855 1105 42-43 0.15339659743580114 1105 44-45 0.5135204496589836 1105 46-47 0.014229005138854944 1105 48-49 0.5935097324833976 1105 50-51 0.5923800321099186 1105 52-53 0.49864443016946325 1105 54-55 -0.21745300043480498 1105 56-57 0.2912258674805699 1105 58-59 -0.26597373041333583 1105 60-61 0.38633557854962675 1105 62-63 0.437824977271255 1105 64-65 0.3017466505390871 1105 66-67 0.495587005860628 1105 68-69 0.6914186047357269 1105 70-71 0.28352650726808903 1105 72-73 0.6479649972208819 1105 74-75 0.500149216967511 1105 76 0.1672306463465425 1106 1 0.4279649873571536 1106 2 0.7754452840922283 1106 3 0.8157057459200026 1106 4 0.9756550634263093 1106 5 0.056088809505006054 1106 6 0.9704092249772991 1106 7 -0.4231834928592413 1106 8 -0.023574109242993302 1106 9 0.6104510911043377 1106 10-11 0.132459057721519 1106 12-13 0.7568200358827681 1106 14-15 0.9184842079230897 1106 16-17 1.2091566950240562 1106 18-19 0.6623392535177146 1106 20-21 0.6326961285404309 1106 22-23 0.43502320770033975 1106 24-25 0.33107776587406335 1106 26-27 0.6628528263035243 1106 28-29 0.688876268161394 1106 30-31 0.8020754817717091 1106 32-33 1.0894046016919532 1106 34-35 0.6493226354622763 1106 36-37 0.7645487946583245 1106 38-39 0.40302691081479836 1106 40-41 0.6599685416682988 1106 42-43 0.3295798912661567 1106 44-45 0.8224876262732224 1106 46-47 0.6426496041476888 1106 48-49 0.4825243126744496 1106 50-51 -0.5110639095693195 1106 52-53 0.2049936365186653 1106 54-55 0.2871257541439505 1106 56-57 0.21135802290684325 1106 58-59 0.6857967213571143 1106 60-61 0.4341521410720688 1106 62-63 0.4802547697010411 1106 64-65 0.722399527074316 1106 66-67 0.2740830196507602 1106 68-69 0.702084409519184 1106 70-71 0.2937793263444348 1106 72-73 0.6117299903976345 1106 74-75 0.15559344594115387 1106 76 0.05418029800207691 1107 1 0.13681795885603876 1107 2 -0.6760329825611358 1107 3 0.2979299635527788 1107 4 -0.234145728086542 1107 5 -0.21635124503621483 1107 6 -0.07827879258231007 1107 7 -0.28624844347282874 1107 8 -0.11981941171397636 1107 9 0.6493615969409134 1107 10-11 0.23998674404298015 1107 12-13 0.37906387505267247 1107 14-15 0.4196814542565228 1107 16-17 0.3624208541247924 1107 18-19 0.5710658490147367 1107 20-21 0.8393790910599677 1107 22-23 0.7730623842609177 1107 24-25 0.9979689162282526 1107 26-27 0.3317061303708364 1107 28-29 0.9073238387490434 1107 30-31 0.17340475777024267 1107 32-33 0.6454839525183758 1107 34-35 0.665668373597903 1107 36-37 0.9661684028927127 1107 38-39 0.20855752373840275 1107 40-41 0.7285857221531771 1107 42-43 0.5587361651695133 1107 44-45 0.18748147375733737 1107 46-47 0.5891726012543437 1107 48-49 0.35415290025634505 1107 50-51 0.47994011450863283 1107 52-53 0.3435582711922578 1107 54-55 0.13093499289482224 1107 56-57 1.0627165649268662 1107 58-59 0.5165443351786578 1107 60-61 0.6508370990021675 1107 62-63 0.6487755053423996 1107 64-65 0.2961798323721325 1107 66-67 0.25804490730915575 1107 68-69 0.2941227214018518 1107 70-71 -0.07453189118857395 1107 72-73 0.7968957757579744 1107 74-75 0.3264089037215889 1107 76 0.25927524628768595 1108 1 -0.3646717111372837 1108 2 -0.3119022823348381 1108 3 -0.05519508732392708 1108 4 0.2276845309369513 1108 5 0.5088656932442319 1108 6 0.030703184852850285 1108 7 -0.09537531777073127 1108 8 -0.3784358908830221 1108 9 -0.6656995365107257 1108 10-11 -0.5288701987960209 1108 12-13 0.10165964536672334 1108 14-15 -0.05158338139135665 1108 16-17 -0.4419358200851953 1108 18-19 -0.22901718177177344 1108 20-21 -0.12397339904959992 1108 22-23 0.419538459998023 1108 24-25 -0.2537039099117955 1108 26-27 -0.2809389699987328 1108 28-29 -0.5768677507207016 1108 30-31 -0.37217263180694715 1108 32-33 -0.6356159611947234 1108 34-35 -0.1425630706493699 1108 36-37 -0.536509746985999 1108 38-39 -0.5049944030642628 1108 40-41 -0.5076368654768615 1108 42-43 -0.01734282804149956 1108 44-45 -0.7299924366670965 1108 46-47 0.16061977902162639 1108 48-49 -0.5409352589022802 1108 50-51 -0.3744548189853347 1108 52-53 -0.3656711705478095 1108 54-55 -0.14587499684843408 1108 56-57 -0.06370187767607405 1108 58-59 -0.42780875731112644 1108 60-61 -0.24513834993389594 1108 62-63 0.022104714270653858 1108 64-65 -0.500484283675604 1108 66-67 -0.7713251983515974 1108 68-69 -0.5256272094058261 1108 70-71 -0.5531880948907677 1108 72-73 -0.7824963086403756 1108 74-75 -0.471476253304278 1108 76 0.18234966466516767 1109 1 -0.31889273008879826 1109 2 -0.47960032389455876 1109 3 -0.3662952596104141 1109 4 -0.17481538116178186 1109 5 -0.6994954754910445 1109 6 -0.43661512322351825 1109 7 -0.12454095892258721 1109 8 -0.2657992044092694 1109 9 -0.6668598332654092 1109 10-11 -0.3353819248253451 1109 12-13 -0.6640483114561704 1109 14-15 -0.38856168735809504 1109 16-17 -0.6160067036687522 1109 18-19 -0.3478596978577073 1109 20-21 -0.8050148532881991 1109 22-23 -0.29296285852101533 1109 24-25 -1.0656039837488613 1109 26-27 -0.2340084823224906 1109 28-29 -0.5376374142346414 1109 30-31 -0.40513560779232094 1109 32-33 -0.5182589765598635 1109 34-35 -0.7120578141534679 1109 36-37 -1.2067217648475363 1109 38-39 -0.4319044976460269 1109 40-41 -0.4591329450802526 1109 42-43 -0.26046894878268034 1109 44-45 -0.26589131504690044 1109 46-47 -0.004678557886357737 1109 48-49 -0.33506169608803305 1109 50-51 -0.21854433763798298 1109 52-53 -0.8059774185700306 1109 54-55 -0.4380412357289245 1109 56-57 -0.6058329560488431 1109 58-59 -0.37521742789232704 1109 60-61 -0.30274005170247165 1109 62-63 -0.7617548546615218 1109 64-65 -0.22346343349452624 1109 66-67 -0.3972701369965108 1109 68-69 -0.4955561851802379 1109 70-71 -0.7584902994546034 1109 72-73 -0.6692618935354204 1109 74-75 -0.4872457410156805 1109 76 -0.9546180931492536 1110 1 -0.37482329903768985 1110 2 -0.21124380095751505 1110 3 -0.029747525762779503 1110 4 -0.27651671743399575 1110 5 -0.41347122972716477 1110 6 -0.16557227985666145 1110 7 0.21899809604366283 1110 8 -0.19006563229189766 1110 9 -0.10676202364975751 1110 10-11 -0.2532597316857945 1110 12-13 0.06556320528166992 1110 14-15 -0.2544350858978426 1110 16-17 -0.3532294616243945 1110 18-19 -0.2600721238116961 1110 20-21 -0.2799597492793815 1110 22-23 -0.6383444537820715 1110 24-25 -0.19361748423101943 1110 26-27 -0.18605077464926012 1110 28-29 -0.9034419327633643 1110 30-31 -1.0105909046651718 1110 32-33 0.20391349898773115 1110 34-35 -0.48806001831381707 1110 36-37 -0.3252298238088258 1110 38-39 -0.5376869745712227 1110 40-41 -0.590504346099678 1110 42-43 -0.32750641950867987 1110 44-45 -0.784420038011497 1110 46-47 -0.8241957090090999 1110 48-49 -0.9261382906602869 1110 50-51 -1.3459034780154475 1110 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-0.04047410249006589 2116 22-23 0.06690466927612704 2116 24-25 -0.46908458996514923 2116 26-27 -0.3892596657653016 2116 28-29 -1.0556092317826185 2116 30-31 -0.5641022512182516 2116 32-33 -0.7587180422822222 2116 34-35 -0.6901503612159559 2116 36-37 -0.764040762315517 2116 38-39 -0.1383831589795541 2116 40-41 -0.4489498325339234 2116 42-43 -0.4175182313778194 2116 44-45 -0.028854142099113744 2116 46-47 -0.14684765259847765 2116 48-49 -0.63272967157517 2116 50-51 -0.7300576765980225 2116 52-53 -0.21033872641194762 2116 54-55 -0.10375992495134057 2116 56-57 -0.586723565567759 2116 58-59 -0.34410847776205955 2116 60-61 -0.09925210630597192 2116 62-63 -0.3931170065091578 2116 64-65 -0.8724946617063338 2116 66-67 -0.5540717222594367 2116 68-69 -0.28078425020537523 2116 70-71 -0.254582185990877 2116 72-73 -0.08381548789893145 2116 74-75 0.10698933292655965 2116 76 -0.4201973379476094 2117 1 -0.64363084451265 2117 2 -1.1349282084730454 2117 3 -1.4471440374777274 2117 4 -0.2330441521222184 2117 5 -1.3258336820065253 2117 6 -1.1447997669440184 2117 7 -0.9028111711170546 2117 8 -0.6151910159283958 2117 9 -1.508270370082876 2117 10-11 -0.4169789471959362 2117 12-13 -0.315097772336415 2117 14-15 -0.5927556937277672 2117 16-17 -0.6386363034538718 2117 18-19 -0.8691558875664711 2117 20-21 -1.871723728619152 2117 22-23 -0.6078874584979914 2117 24-25 -0.3205730946809098 2117 26-27 -0.3613539410539275 2117 28-29 -0.5894869211606988 2117 30-31 -0.6023268130415431 2117 32-33 -1.0095006787201797 2117 34-35 -0.45441229019045437 2117 36-37 -1.092259541602072 2117 38-39 -0.6218530798186208 2117 40-41 -0.6040813353952856 2117 42-43 -0.05515257565809151 2117 44-45 -0.7340045877532404 2117 46-47 0.13632715946907936 2117 48-49 -0.6347336196519748 2117 50-51 -0.5108297449515966 2117 52-53 -0.47004441523170826 2117 54-55 -0.7655612848444591 2117 56-57 -0.9314823939061796 2117 58-59 -0.862353378162851 2117 60-61 -0.670636525360429 2117 62-63 -0.5951169666295968 2117 64-65 -0.7739123801964958 2117 66-67 -0.3215439561131994 2117 68-69 -0.7875538061464304 2117 70-71 -0.5497108972827753 2117 72-73 -1.6683601629801856 2117 74-75 -1.62244776703157 2117 76 -1.2832509161414656 2118 1 0.8291326772572383 2118 2 0.280982782587472 2118 3 1.235472207939985 2118 4 0.008579103392605703 2118 5 0.8875562466062448 2118 6 0.7548079248689596 2118 7 1.4000378867001686 2118 8 1.0393848410398974 2118 9 0.9766482742029297 2118 10-11 -0.14950193902820885 2118 12-13 0.5326416321268965 2118 14-15 0.6351689316500675 2118 16-17 0.5268405760882189 2118 18-19 0.668177703018138 2118 20-21 0.23384576138020208 2118 22-23 0.6849931125998268 2118 24-25 0.3987014567371929 2118 26-27 0.15054393019180523 2118 28-29 0.8351384566771003 2118 30-31 0.5997762160921596 2118 32-33 0.3794310853804035 2118 34-35 -0.027696745782456844 2118 36-37 0.43169698293326775 2118 38-39 0.2357583421377747 2118 40-41 0.6197915824772551 2118 42-43 0.7973781337122858 2118 44-45 0.5651143266464018 2118 46-47 0.4570230241549105 2118 48-49 0.3179643030440147 2118 50-51 0.33809944663628855 2118 52-53 0.32627689160474915 2118 54-55 0.7981405609334118 2118 56-57 0.5034008783299768 2118 58-59 0.5671919302171062 2118 60-61 0.7974000043199396 2118 62-63 0.1628417445415451 2118 64-65 0.43944539200750654 2118 66-67 0.4054481334102462 2118 68-69 0.13906526903525318 2118 70-71 0.7899572485786912 2118 72-73 0.6339100794791364 2118 74-75 0.5492674558123447 2118 76 0.09053517942738054 2119 1 1.0674417163535352 2119 2 0.7094019145554071 2119 3 -0.08322307877301327 2119 4 0.6383841344523269 2119 5 0.050788917742231376 2119 6 -0.9746137287792749 2119 7 0.40063242920995634 2119 8 -0.04186246814271044 2119 9 -0.18430873422317262 2119 10-11 0.12616077085554878 2119 12-13 0.31613590196718633 2119 14-15 0.2802578055045508 2119 16-17 0.4214322893957245 2119 18-19 -0.37462935768670746 2119 20-21 -0.34396755227172093 2119 22-23 0.20227584831543055 2119 24-25 0.369343357633106 2119 26-27 -0.3499583557216326 2119 28-29 -0.051242419555549645 2119 30-31 -0.721307491392924 2119 32-33 -0.48360887197654634 2119 34-35 0.00417278288884404 2119 36-37 0.26216011104692427 2119 38-39 0.3769006838152045 2119 40-41 0.1362030058940249 2119 42-43 0.3685079694955462 2119 44-45 0.054115290215293044 2119 46-47 -0.1293804036901811 2119 48-49 0.19259783934186459 2119 50-51 0.2109903583364776 2119 52-53 -0.4330461499927303 2119 54-55 -0.15179485944915427 2119 56-57 -0.18279343456339348 2119 58-59 -0.1454185214301802 2119 60-61 0.05140291552808662 2119 62-63 0.2946559192550353 2119 64-65 -0.13231998921725108 2119 66-67 0.1577186707536633 2119 68-69 -0.2917012807730579 2119 70-71 -0.6825297842441529 2119 72-73 -0.16241810798626943 2119 74-75 -0.23362202078522998 2119 76 -0.3852628401310163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 15.0 13 255.0 14 1000.0 15 1903.0 16 2574.0 17 3015.0 18 3144.0 19 2836.0 20 2359.0 21 1658.0 22 1131.0 23 837.0 24 776.0 25 736.0 26 734.0 27 774.0 28 962.0 29 1108.0 30 1308.0 31 1606.0 32 2039.0 33 2644.0 34 3730.0 35 5482.0 36 10276.0 37 36653.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.722020612570766 19.794097613810198 11.964447223555947 33.51943455006309 2 18.585928514800628 18.802550331074062 34.749935795080226 27.861585359045076 3 17.90368145426935 20.412698058219906 31.269470839800352 30.4141496477104 4 14.69248291571754 21.51949618116039 29.157175398633257 34.63084550448881 5 16.56375269381511 31.660283394932836 31.269470839800352 20.5064930714517 6 28.056991636611322 30.01663744877564 17.162254206818 24.764116707795036 7 26.206773339884098 28.26691380908248 19.181080205902386 26.345232645131034 8 22.998760565896582 33.33854416740177 18.284444543698427 25.378250723003227 9 22.82233661243677 18.201815603470415 19.13976573578838 39.836082048304434 10-11 25.113056489163327 23.53696528467903 24.095268934868297 27.25470929128935 12-13 23.850173632435208 20.44061324072937 24.332547986198733 31.37666514063669 14-15 23.8507319360854 21.510881338142188 22.312605379813974 32.32578134595844 16-17 25.286130620722 22.62581372757015 22.48232968947151 29.605725962236342 18-19 23.77536094330985 21.735319405518272 23.801601214868743 30.687718436303136 20-21 24.070145270609782 22.165771519814196 23.807184251370636 29.956898958205393 22-23 26.037049030226562 21.91118505532789 22.37625199593555 29.67551391851 24-25 24.391728173118796 23.66816664247351 21.710195741259756 30.22990944314794 26-27 23.70668959433657 23.75135388635171 23.592237346047767 28.949719173263954 28-29 24.377212278213875 22.91557332201838 25.04606005114061 27.661154348627132 30-31 24.295277283055945 22.135746707385838 25.00321026391161 28.565765745646605 32-33 23.10483825943254 23.220407115021718 25.178936319885658 28.49581830566008 34-35 23.383990084527174 23.16625166095336 25.021494690532286 28.428263563987183 36-37 23.866364438290695 21.787241644985876 25.613296559732905 28.733097356990523 38-39 22.56285138432163 21.42613880622847 27.818751291084396 28.1922585183655 40-41 22.343312080574382 21.283093448864967 28.2244827316681 28.14911173889255 42-43 20.796368772785893 21.41218113704756 27.99629284306555 29.79515724710099 44-45 19.936018044474718 21.006850424033992 29.864387286238298 29.192744245252992 46-47 20.480811103542994 22.098216778141293 29.663231238205835 27.75774088010988 48-49 20.021885503087418 22.80782071753185 28.794510758511336 28.375783020869388 50-51 20.216733477003473 23.755820315553223 27.012405507107207 29.015040700336098 52-53 18.9359849034693 23.954018111370413 27.773931685965366 29.336065299194924 54-55 19.06941947586453 23.01271815715131 27.6834864946347 30.234375872349457 56-57 19.5858503522896 23.716739060039973 26.885112274864053 29.81229831280637 58-59 19.95153924316357 23.554831001485088 26.201748607032393 30.29188114831895 60-61 19.722634746585975 22.365085922931762 26.781826099579035 31.13045323090322 62-63 19.74887501814487 22.844110454794155 26.119677970454568 31.287336556606405 64-65 20.033051576091204 23.269537836238374 25.0935158614067 31.60389472626372 66-67 20.379871924427594 24.82511040516322 23.310982452418305 31.48403521799088 68-69 19.965496834418303 25.783579173040632 23.42642116194156 30.824502830599503 70-71 20.33174402894246 26.573020534408254 22.980894849090525 30.114340587558765 72-73 21.040789664682826 26.298893442165326 22.994852440345255 29.665464452806596 74-75 20.243532052212558 27.21395312482553 24.534653907567247 28.007860915394666 76 20.97681897360311 26.135602304703205 24.496404484344993 28.3911742373487 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 1.5 14 2.0 15 3.5 16 7.0 17 10.0 18 13.0 19 20.0 20 24.0 21 24.0 22 34.0 23 65.5 24 107.0 25 127.0 26 155.0 27 234.5 28 346.0 29 406.0 30 461.0 31 619.0 32 862.0 33 1002.0 34 1195.5 35 1509.5 36 1775.5 37 1921.0 38 2210.5 39 2821.0 40 3395.5 41 3734.5 42 3820.0 43 4024.0 44 4325.0 45 4519.5 46 4617.0 47 4598.5 48 4481.5 49 4066.5 50 3750.0 51 3590.0 52 3107.0 53 2603.0 54 2422.0 55 2428.0 56 2371.5 57 2306.5 58 2304.0 59 2294.5 60 2349.5 61 2439.0 62 2464.0 63 2524.0 64 2546.0 65 2503.5 66 2441.5 67 2384.0 68 2327.0 69 2157.5 70 1845.0 71 1645.0 72 1440.5 73 1057.5 74 734.5 75 590.0 76 445.0 77 209.5 78 80.5 79 42.0 80 30.5 81 17.5 82 12.0 83 8.0 84 8.0 85 5.5 86 4.0 87 5.0 88 5.0 89 5.0 90 3.5 91 1.5 92 1.0 93 2.5 94 5.0 95 5.5 96 5.0 97 3.5 98 4.5 99 16.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0011166073003785297 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 5.583036501892649E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.583036501892649E-4 40-41 0.0 42-43 5.583036501892649E-4 44-45 5.583036501892649E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.583036501892649E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0011166073003785297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 89557.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.8286342776109 #Duplication Level Percentage of deduplicated Percentage of total 1 79.62069617858494 56.39425170561765 2 11.748013620885358 16.64191520484161 3 4.265985622398789 9.064618064472905 4 1.7956236599823434 5.087262860524582 5 0.9443183251355782 3.3442388646336965 6 0.5407365367637785 2.2979778241790147 7 0.340522133938706 1.6883102381723372 8 0.2364737041241014 1.3399287604542358 9 0.16080211880438897 1.0250455017474904 >10 0.3468280993820154 3.116450975356477 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005583036501892649 0.0 2 0.0 0.0 0.0 0.00893285840302824 0.0 3 0.0 0.0 0.0 0.00893285840302824 0.0 4 0.0 0.0 0.0 0.04578089931551973 0.0 5 0.0 0.0 0.0 0.05359715041816943 0.0 6 0.0 0.0 0.0 0.05583036501892649 0.0 7 0.0 0.0 0.0 0.056946972319305025 0.0 8 0.0 0.0 0.0 0.059180186920062085 0.0 9 0.0 0.0 0.0 0.0669964380227118 0.0 10 0.0 0.0 0.0 0.0669964380227118 0.0 11 0.0 0.0 0.0 0.0669964380227118 0.0 12 0.0 0.0 0.0 0.06922965262346885 0.0 13 0.0 0.0 0.0 0.07146286722422591 0.0 14 0.0 0.0 0.0 0.07816251102649709 0.0 15 0.0 0.0 0.0 0.08151233292763269 0.0 16 0.0 0.0 0.0 0.08151233292763269 0.0 17 0.0 0.0 0.0 0.08151233292763269 0.0 18 0.0 0.0 0.0 0.09379501323179651 0.0 19 0.0 0.0 0.0 0.10942751543709593 0.0 20 0.0 0.0 0.0 0.11389394463861005 0.0 21 0.0 0.0 0.0 0.1194769811405027 0.0 22 0.0 0.0 0.0 0.130643054144288 0.0 23 0.0 0.0 0.0 0.13622609064618063 0.0 24 0.0 0.0 0.0 0.1529752001518586 0.0 25 0.0 0.0 0.0 0.1607914512545083 0.0 26 0.0 0.0 0.0 0.1797737753609433 0.0 27 0.0 0.0 0.0 0.30036736380182455 0.0 28 0.0 0.0 0.0 0.43547684714762663 0.0 29 0.0 0.0 0.0 0.558303650189265 0.0 30 0.0 0.0 0.0 0.6733142021282535 0.0 31 0.0 0.0 0.0 0.8408052971850329 0.0 32 0.0 0.0 0.0 1.0462610404546826 0.0 33 0.0 0.0 0.0 1.3399287604542358 0.0 34 0.0 0.0 0.0 1.679377379769309 0.0 35 0.0 0.0 0.0 2.14946905322867 0.0 36 0.0 0.0 0.0 2.84064897216298 0.0 37 0.0 0.0 0.0 3.6580055160400637 0.0 38 0.0 0.0 0.0 4.5613408220462945 0.0 39 0.0 0.00223321460075706 0.0 5.456859876949875 0.0 40 0.0 0.00223321460075706 0.0 6.199403731701598 0.0 41 0.0 0.00223321460075706 0.0 7.058074745692688 0.0 42 0.0 0.00223321460075706 0.0 8.015007202117088 0.0 43 0.0 0.00223321460075706 0.0 8.910526257020669 0.0 44 0.0 0.00223321460075706 0.0 9.779246736715164 0.0 45 0.0 0.00223321460075706 0.0 10.697097937626316 0.0 46 0.0 0.00223321460075706 0.0 11.631698248043145 0.0 47 0.0 0.00223321460075706 0.0 12.51716783724332 0.0 48 0.0 0.00223321460075706 0.0 13.346807061424567 0.0 49 0.0 0.00223321460075706 0.0 14.000022332146008 0.0 50 0.0 0.00223321460075706 0.0 14.724700470091673 0.0 51 0.0 0.00223321460075706 0.0 15.412530567124849 0.0 52 0.0 0.00223321460075706 0.0 16.098127449557264 0.0 53 0.0 0.00223321460075706 0.0 16.732360396172272 0.0 54 0.0 0.00223321460075706 0.0 17.375526201190304 0.0 55 0.0 0.00223321460075706 0.0 18.006409325904173 0.0 56 0.0 0.00223321460075706 0.0 18.67525709883091 0.0 57 0.0 0.00223321460075706 0.0 19.27040878993267 0.0 58 0.0 0.00223321460075706 0.0 19.875609946737832 0.0 59 0.0 0.00223321460075706 0.0 20.510959500653215 0.0 60 0.0 0.00223321460075706 0.0 21.192089953884118 0.0 61 0.0 0.00223321460075706 0.0 21.750393604073384 0.0 62 0.0 0.00223321460075706 0.0 22.372343870384224 0.0 63 0.0 0.00223321460075706 0.0 22.913898411067812 0.0 64 0.0 0.00223321460075706 0.0 23.488951170762753 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATTAA 15 0.002218193 70.0 23 CCGTAAA 25 2.3791096E-4 55.999996 16 GACGCTT 40 4.5607845E-5 43.750004 14 CGTTGCT 35 0.0012495791 40.000004 28 TCACATA 40 0.00240758 35.000004 51 TGGTGTA 40 0.00240758 35.000004 34 CTCTAAT 40 0.00240758 35.000004 41 TCCACAT 95 7.477865E-9 33.157898 5 ATCCACA 100 1.2330929E-8 31.499998 4 AATGATT 45 0.0042874827 31.111113 25 GATCTCT 45 0.0042874827 31.111113 41 TACAACG 45 0.0042874827 31.111113 22 TATCCAC 90 1.7281855E-7 31.111113 3 ACGCTTC 70 3.455401E-5 30.000002 15 TTATCCA 120 2.242814E-9 29.166666 2 TGTAGAT 85 3.905663E-6 28.82353 37 CGACGAT 50 0.0071754744 27.999998 22 CTTATAA 50 0.0071754744 27.999998 1 TCTAATA 50 0.0071754744 27.999998 42 TAGATCT 110 9.994837E-7 25.454548 39 >>END_MODULE