FastQCFastQC Report
Wed 25 May 2016
SRR1780294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89557
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT940610.502808267360452No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC25602.8585146889690365No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG25032.79486807284746No Hit
CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG21192.366090869502105RNA PCR Primer, Index 47 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5820.6498654488203044No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT3120.3483814777181013No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2980.3327489755128019Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT1900.21215538707192066No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1760.19652288486662126No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC1680.18759002646359302No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1240.1384593052469377No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG1040.1161271592393671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT950.066.3157961
CTTTATA1100.057.272732
TCTAGCA200.00693122652.5000043
AGGCATA200.00693122652.50000439
TCGCTAA200.00693122652.50000414
CCGAGAG200.00693122652.50000429
CTCGCTA200.00693122652.50000413
TGCAGAA200.00693122652.50000418
TGTAAAG200.00693122652.50000448
TTTATAC1250.050.43
AACTCGT350.001249579140.00000437
TGCTTGA21050.036.57957555
CTTGAAA20800.036.34615357
GCTTGAA21000.036.33333256
CTGCTTG21100.036.32701554
TTCTGCT21100.036.32701552
TCTGCTT21100.036.32701553
TTGAAAA20950.036.25298358
GTCTTCT21300.036.15023449
CCGTCTT21250.036.07058747
Produced by FastQC (version 0.11.5)