##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780294_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 89557 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.71221680047344 34.0 34.0 34.0 34.0 34.0 2 33.72849693491296 34.0 34.0 34.0 34.0 34.0 3 33.73735163080496 34.0 34.0 34.0 34.0 34.0 4 33.763558404144845 34.0 34.0 34.0 34.0 34.0 5 33.747535089384414 34.0 34.0 34.0 34.0 34.0 6 37.509641904038766 38.0 38.0 38.0 37.0 38.0 7 37.51292472950188 38.0 38.0 38.0 37.0 38.0 8 37.42959232667463 38.0 38.0 38.0 37.0 38.0 9 37.43290865035676 38.0 38.0 38.0 37.0 38.0 10-11 37.399304353651864 38.0 38.0 38.0 37.0 38.0 12-13 37.362043168038234 38.0 38.0 38.0 37.0 38.0 14-15 37.298368636734146 38.0 38.0 38.0 36.0 38.0 16-17 37.30478354567482 38.0 38.0 38.0 36.0 38.0 18-19 37.22810612235783 38.0 38.0 38.0 36.0 38.0 20-21 37.292964257400314 38.0 38.0 38.0 36.0 38.0 22-23 37.29453309065735 38.0 38.0 38.0 36.0 38.0 24-25 37.28224482731668 38.0 38.0 38.0 36.0 38.0 26-27 37.15725738914881 38.0 38.0 38.0 36.0 38.0 28-29 37.16209788179595 38.0 38.0 38.0 36.0 38.0 30-31 37.142691246915376 38.0 38.0 38.0 36.0 38.0 32-33 37.01014996036044 38.0 38.0 38.0 36.0 38.0 34-35 37.04930937838472 38.0 38.0 38.0 36.0 38.0 36-37 37.02229864778856 38.0 38.0 38.0 36.0 38.0 38-39 35.99157519791865 38.0 37.0 38.0 33.0 38.0 40-41 36.530103732818205 38.0 37.0 38.0 34.0 38.0 42-43 36.978656051453264 38.0 37.0 38.0 36.0 38.0 44-45 37.10612235782797 38.0 38.0 38.0 36.0 38.0 46-47 37.04595955648358 38.0 38.0 38.0 36.0 38.0 48-49 37.05253637348281 38.0 38.0 38.0 36.0 38.0 50-51 36.875827685161404 38.0 37.5 38.0 35.0 38.0 52-53 36.8537076945409 38.0 38.0 38.0 35.0 38.0 54-55 36.83555165983675 38.0 37.5 38.0 35.0 38.0 56-57 36.89524548611499 38.0 37.5 38.0 35.0 38.0 58-59 36.74065120537758 38.0 37.0 38.0 34.5 38.0 60-61 36.712847683598156 38.0 37.0 38.0 34.0 38.0 62-63 36.72692810165593 38.0 37.0 38.0 34.0 38.0 64-65 36.59606172605156 38.0 37.0 38.0 34.5 38.0 66-67 36.17040544011077 38.0 37.0 38.0 33.5 38.0 68-69 36.001842402045625 38.0 37.0 38.0 32.5 38.0 70-71 34.9168183391583 38.0 36.0 38.0 26.5 38.0 72-73 33.05200598501513 38.0 32.5 38.0 16.0 38.0 74-75 32.551676585861514 38.0 31.0 38.0 11.0 38.0 76 31.947809774780307 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.015478442817936866 1101 2 0.1299606990523543 1101 3 0.06769962198117696 1101 4 0.11699027002914164 1101 5 0.15090984476006497 1101 6 0.1717418386962848 1101 7 0.2078152213707085 1101 8 0.22762521563634408 1101 9 0.17127073808646998 1101 10-11 0.1613164665035285 1101 12-13 0.12994697875400618 1101 14-15 0.25590038850086927 1101 16-17 0.2893400448457797 1101 18-19 0.1558571233792989 1101 20-21 -0.021949322282800665 1101 22-23 0.07249328951230893 1101 24-25 0.21771980014094083 1101 26-27 -0.020785174809560658 1101 28-29 -0.16679499590717484 1101 30-31 0.06039035891462419 1101 32-33 -0.03666888843967797 1101 34-35 0.1747786312726589 1101 36-37 0.27801665946342524 1101 38-39 0.6934235051251747 1101 40-41 0.6613155312426215 1101 42-43 0.18875918473333542 1101 44-45 0.17858347160056098 1101 46-47 0.2796021294801392 1101 48-49 0.2688042737629601 1101 50-51 0.02279372602775709 1101 52-53 0.11919430178050305 1101 54-55 0.19059368759703688 1101 56-57 0.33118132151232516 1101 58-59 0.3845458067414924 1101 60-61 0.2905858116723863 1101 62-63 0.2513653142714176 1101 64-65 -0.03216539494585646 1101 66-67 0.042237535126822934 1101 68-69 0.00824543858622917 1101 70-71 -0.01649018402218161 1101 72-73 0.058096159864170716 1101 74-75 0.3709685157510805 1101 76 -1.0871427553685074 1102 1 0.10068825548829352 1102 2 0.0936579854635724 1102 3 0.05266463873493876 1102 4 -0.2845271174255046 1102 5 -0.25907991200654124 1102 6 0.033301935225736656 1102 7 0.08576449132331021 1102 8 -0.03097302391642387 1102 9 -0.024704886490340527 1102 10-11 -0.08424335224331969 1102 12-13 -0.03806809095205921 1102 14-15 0.11030758307021671 1102 16-17 0.22419134434750987 1102 18-19 0.1465774483849458 1102 20-21 0.03003074681562623 1102 22-23 0.03849096308302791 1102 24-25 0.04124972253543291 1102 26-27 0.21278658858911115 1102 28-29 0.27511371778577143 1102 30-31 0.21634792762241517 1102 32-33 0.023826953502023684 1102 34-35 0.2562036560126728 1102 36-37 0.41858333596822916 1102 38-39 0.2598743115627826 1102 40-41 0.11614981656006762 1102 42-43 0.2102265713199074 1102 44-45 0.3069103522755725 1102 46-47 0.3475373366885961 1102 48-49 0.3450208747456145 1102 50-51 0.22485452569442543 1102 52-53 0.4078017428819152 1102 54-55 0.24012579752949392 1102 56-57 0.3146792989077696 1102 58-59 0.5473003684526319 1102 60-61 0.2777036435791942 1102 62-63 0.39662825286411163 1102 64-65 0.3364868385998605 1102 66-67 0.7228135867943948 1102 68-69 0.6679096688680062 1102 70-71 0.5523912201688645 1102 72-73 0.7512765970245354 1102 74-75 0.8024343398105316 1102 76 0.17506665798936183 1103 1 -0.14296863860054287 1103 2 0.04646731816202987 1103 3 0.042869386711267055 1103 4 -0.03634672153406626 1103 5 0.14468513799005933 1103 6 -0.17420095573406513 1103 7 0.09496454574137658 1103 8 0.02555374297018176 1103 9 -0.17330022014111535 1103 10-11 -0.12946136462343105 1103 12-13 -0.22652465853249737 1103 14-15 0.11155749804198223 1103 16-17 0.05572491349246178 1103 18-19 0.1216726800018435 1103 20-21 0.06951785653605214 1103 22-23 0.1473441023252562 1103 24-25 -0.0038917644382436833 1103 26-27 0.1696942819433005 1103 28-29 -0.08072942213667744 1103 30-31 -0.1036898249114202 1103 32-33 0.29816203274832986 1103 34-35 -0.013505887555794516 1103 36-37 0.06056347337383983 1103 38-39 0.01017191036558529 1103 40-41 0.09589609232362761 1103 42-43 -0.04204368240432643 1103 44-45 0.23008734703783063 1103 46-47 0.04161427023053221 1103 48-49 0.3340522331566049 1103 50-51 -0.3706831928281247 1103 52-53 -0.00524838976212294 1103 54-55 0.01986867216387367 1103 56-57 -0.001574697419016502 1103 58-59 0.0948311486716662 1103 60-61 0.04975996627832302 1103 62-63 -0.1522374705560665 1103 64-65 0.34944514075618116 1103 66-67 -0.030315452046501434 1103 68-69 -0.11901809114831963 1103 70-71 0.08085016853971894 1103 72-73 0.28510932949171774 1103 74-75 0.49989539688147033 1103 76 0.7959768001565592 1104 1 -0.29111929569307904 1104 2 -0.033772486742449814 1104 3 0.053504801966653304 1104 4 0.027533511496685037 1104 5 -0.029715349748734354 1104 6 0.051716941569821984 1104 7 -0.048884526200097866 1104 8 -0.11114860088566303 1104 9 -0.0181721887090589 1104 10-11 0.20152359676391285 1104 12-13 0.12380050066002468 1104 14-15 0.17684336716541083 1104 16-17 -0.106532910650607 1104 18-19 0.002532319617067458 1104 20-21 0.09288867349852126 1104 22-23 -0.23139861546366092 1104 24-25 -0.04485843562101621 1104 26-27 0.15147181886241867 1104 28-29 -0.09654706202287855 1104 30-31 -0.037218479565261475 1104 32-33 -0.06923398405156433 1104 34-35 -0.09615051565496913 1104 36-37 0.02828464936626318 1104 38-39 0.07677873870863294 1104 40-41 -0.1241282946158151 1104 42-43 0.217728028745924 1104 44-45 0.1481709400469171 1104 46-47 -0.16643680685928786 1104 48-49 0.09546667564407585 1104 50-51 -0.45532619513133454 1104 52-53 -0.1717908636816432 1104 54-55 -0.07839037132616511 1104 56-57 -0.5064859560560606 1104 58-59 0.043991154090981865 1104 60-61 -0.1274179228965835 1104 62-63 -0.09569025473165738 1104 64-65 -0.08271174855421037 1104 66-67 -0.5454556769482792 1104 68-69 -0.4113057215507041 1104 70-71 -0.7431404831334945 1104 72-73 -0.4880413276008184 1104 74-75 -0.2634327429369847 1104 76 -0.8999993548359875 1105 1 -0.26715929847105 1105 2 -0.08065074217685719 1105 3 -0.3070410692744048 1105 4 -0.09778769136462273 1105 5 0.1379004569045037 1105 6 0.006540193184520149 1105 7 -0.024507833374208587 1105 8 -0.2191769361867273 1105 9 -0.02362395619291391 1105 10-11 0.15119024028081895 1105 12-13 0.029089062365699192 1105 14-15 -0.06114190958991372 1105 16-17 0.010350206232509152 1105 18-19 0.11118569832392211 1105 20-21 -0.07767872701540313 1105 22-23 0.09706169428007172 1105 24-25 -0.1372606857772709 1105 26-27 0.15192323909992922 1105 28-29 0.07900332342055805 1105 30-31 0.13058831257307446 1105 32-33 0.030390989904958587 1105 34-35 -0.0807327783120968 1105 36-37 -0.08201814943921448 1105 38-39 -0.015640873764461105 1105 40-41 0.08649397081928356 1105 42-43 0.18104145328929633 1105 44-45 -0.04080051630422332 1105 46-47 -0.28000372122834705 1105 48-49 0.12937528040828994 1105 50-51 -0.2339219309463232 1105 52-53 0.09386994609680954 1105 54-55 -0.2042324436923053 1105 56-57 0.2082338161503472 1105 58-59 0.290588139992785 1105 60-61 0.03240220350108558 1105 62-63 0.15891075922764486 1105 64-65 -0.20831074848475595 1105 66-67 -0.2256591633014935 1105 68-69 0.0797179606494467 1105 70-71 -0.20997845799285386 1105 72-73 0.6745741318060823 1105 74-75 0.047474264494091756 1105 76 0.845226008036434 1106 1 -0.05434596801065794 1106 2 0.016167472288415752 1106 3 -0.004699619874131145 1106 4 0.02474337234291113 1106 5 0.1488535560929023 1106 6 0.01964800040997261 1106 7 -0.14032339036623398 1106 8 0.1819219649121706 1106 9 0.18868660905882706 1106 10-11 0.07135830750771532 1106 12-13 -0.12227917723783577 1106 14-15 -0.3521352299949996 1106 16-17 -0.02048007459777068 1106 18-19 0.12312637202930432 1106 20-21 1.9601262404478348E-4 1106 22-23 -0.2363255067541914 1106 24-25 0.11546861106966588 1106 26-27 -0.5463278562099845 1106 28-29 -0.06423089334307264 1106 30-31 -0.36039782547188537 1106 32-33 -0.366578054122904 1106 34-35 0.06128043723053622 1106 36-37 -0.1448099328192356 1106 38-39 -0.14540816529645895 1106 40-41 -0.43286248971364927 1106 42-43 -0.17100192816585746 1106 44-45 -0.10375390278701957 1106 46-47 -0.18164129933651196 1106 48-49 -0.32487308560477857 1106 50-51 -0.3062303414900356 1106 52-53 -0.20830990020068185 1106 54-55 -0.10360757836155443 1106 56-57 0.10978161475696879 1106 58-59 -0.2236149771515059 1106 60-61 -0.24889493073722946 1106 62-63 -0.3042796451392249 1106 64-65 -0.4621353552505454 1106 66-67 -0.30091234149585233 1106 68-69 0.06402450377952107 1106 70-71 0.05081303809275539 1106 72-73 0.673945675295279 1106 74-75 0.40319860551255005 1106 76 0.7868693320327012 1107 1 -0.28094146503417505 1107 2 -0.1880960549323092 1107 3 -0.10977461405418154 1107 4 0.03959255256174288 1107 5 0.00979006878038291 1107 6 -0.05501361121661574 1107 7 -0.26583471432113726 1107 8 0.014008166648181941 1107 9 0.19823132288370005 1107 10-11 -0.05120978587749647 1107 12-13 0.09716609862767456 1107 14-15 0.13998289830989563 1107 16-17 -0.028977197880884376 1107 18-19 -0.20650713756528916 1107 20-21 -0.030724536778997447 1107 22-23 0.08902618435626408 1107 24-25 0.09801708291775668 1107 26-27 0.08862009683135597 1107 28-29 0.06277698852861846 1107 30-31 0.08868594851461609 1107 32-33 0.2949088593095297 1107 34-35 0.1032256299539398 1107 36-37 0.03244903821406098 1107 38-39 0.27759392988462395 1107 40-41 -0.29574452769296045 1107 42-43 0.09950922950483232 1107 44-45 0.23438504430134088 1107 46-47 -0.13148166649205706 1107 48-49 0.09298251318467265 1107 50-51 0.16781269286428824 1107 52-53 0.16584620741846123 1107 54-55 0.23119737025661635 1107 56-57 -0.11742913385455722 1107 58-59 -0.04730382613218609 1107 60-61 -0.43486719453050426 1107 62-63 0.09601634075986709 1107 64-65 0.16863886404507156 1107 66-67 0.25106810348069786 1107 68-69 0.2721042536880631 1107 70-71 0.5337190209987384 1107 72-73 0.635949732630074 1107 74-75 0.5960633499337149 1107 76 0.238663538690723 1108 1 -0.19791067852097655 1108 2 2.5358608164083307E-4 1108 3 0.009122216123437 1108 4 0.033308261660941696 1108 5 -0.05829517616043489 1108 6 -0.08767238724514215 1108 7 0.002794300868615096 1108 8 -0.06107771323394928 1108 9 -0.10487988952858984 1108 10-11 0.014872951859182137 1108 12-13 -0.1880446530451536 1108 14-15 0.07389202030002906 1108 16-17 0.052938371205613066 1108 18-19 0.22698683049227242 1108 20-21 0.040812183993352846 1108 22-23 0.12688659495163535 1108 24-25 0.07127628549658738 1108 26-27 0.19469599673855242 1108 28-29 0.036133874385711806 1108 30-31 0.18800542167613088 1108 32-33 0.12023487725069515 1108 34-35 -0.03233433943864128 1108 36-37 0.12529699419145857 1108 38-39 -0.003229214540105829 1108 40-41 -0.02278488716742544 1108 42-43 0.07160437301784839 1108 44-45 0.027955856537801083 1108 46-47 0.3423636357562927 1108 48-49 -0.08893631201109997 1108 50-51 0.13576443732483767 1108 52-53 0.21333656119472977 1108 54-55 0.12992423153009014 1108 56-57 0.1617252545667185 1108 58-59 0.08119852640675873 1108 60-61 0.18179920079372636 1108 62-63 -0.062442217109328624 1108 64-65 0.20214422848510338 1108 66-67 -0.22345284144221012 1108 68-69 -0.27627338986565064 1108 70-71 -0.12527679490084864 1108 72-73 -1.0653348024789224 1108 74-75 -0.8821695595627261 1108 76 -0.810992127753444 1109 1 0.06897515531046139 1109 2 -0.06384401948778162 1109 3 0.03749674585164797 1109 4 -0.36249357371756474 1109 5 -0.05537685401986181 1109 6 -0.45144300009278737 1109 7 0.01750897334848389 1109 8 -0.3662357597161403 1109 9 -0.09505252762148331 1109 10-11 -0.01687698660993675 1109 12-13 -0.02553278637380174 1109 14-15 -0.025427623875629024 1109 16-17 -0.3635993735994205 1109 18-19 0.06286637059283606 1109 20-21 0.06727925617786923 1109 22-23 -0.3924341040392534 1109 24-25 0.05391578481096104 1109 26-27 -0.21152213905132555 1109 28-29 -0.021837012713895376 1109 30-31 -0.05638647734289037 1109 32-33 -0.1586846403471327 1109 34-35 -0.11854790436251506 1109 36-37 -0.09042151078374872 1109 38-39 -0.11017868889050675 1109 40-41 -0.06686737371853013 1109 42-43 0.0907053188355107 1109 44-45 -0.013489591934472855 1109 46-47 0.04982821154476369 1109 48-49 -0.1542381649698612 1109 50-51 -0.2276194099379225 1109 52-53 -0.3788191295334471 1109 54-55 -0.22103916638138088 1109 56-57 0.04016658925959149 1109 58-59 -0.2808404314357844 1109 60-61 -0.05163140994429938 1109 62-63 -0.19613125757907568 1109 64-65 -0.23352083251837286 1109 66-67 -0.2907852137216693 1109 68-69 -0.3845677536362686 1109 70-71 0.24380305461217944 1109 72-73 -0.9472746076897138 1109 74-75 -0.19832405483363402 1109 76 -0.24300928608121097 1110 1 -0.011821097616937948 1110 2 -0.211729403149441 1110 3 -0.05549126902643309 1110 4 0.1111942620724875 1110 5 0.029931039629403244 1110 6 -0.182397677045536 1110 7 0.08676782442989861 1110 8 0.14440620198657683 1110 9 0.1791587962523309 1110 10-11 0.09799765177000808 1110 12-13 0.23454091523799292 1110 14-15 0.0808197931239576 1110 16-17 0.15818392988590801 1110 18-19 0.308148089837907 1110 20-21 0.1453375286671985 1110 22-23 0.051032626915421986 1110 24-25 0.20307544867650762 1110 26-27 0.20043198686133223 1110 28-29 0.15492631556823966 1110 30-31 -0.06680457900979064 1110 32-33 0.23053908192865435 1110 34-35 0.2610842763786323 1110 36-37 0.21425199796400562 1110 38-39 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0.3793711949117835 2115 72-73 0.15155622899278143 2115 74-75 0.01694814100122244 2115 76 -0.021343228353771337 2116 1 -0.057350484115616496 2116 2 0.14194659370072316 2116 3 0.12497977399740279 2116 4 0.08130665379702151 2116 5 0.08120528975467778 2116 6 -0.05611805788883828 2116 7 0.055287403497835896 2116 8 0.06872075564109537 2116 9 -0.25601909765561004 2116 10-11 0.01677315466928775 2116 12-13 0.01841055515495782 2116 14-15 0.1115306528848592 2116 16-17 -0.19099102895152242 2116 18-19 -0.024570787676587713 2116 20-21 -0.03346016622865022 2116 22-23 -0.1961844251283864 2116 24-25 -0.0676669094020923 2116 26-27 -0.3066103926934005 2116 28-29 -0.09255918804691277 2116 30-31 -0.23624226245168956 2116 32-33 -0.13010770739055033 2116 34-35 -0.22529628056889806 2116 36-37 -0.1559695640662042 2116 38-39 -0.3090796466535295 2116 40-41 -0.546436930799004 2116 42-43 -0.03992291499076828 2116 44-45 -0.3416518612089945 2116 46-47 -0.13682548923686255 2116 48-49 -0.4160909410147795 2116 50-51 -0.2063908311701681 2116 52-53 -0.34050860881860956 2116 54-55 -0.04384383409999515 2116 56-57 -0.33962681281740004 2116 58-59 -0.05265614462062729 2116 60-61 -0.13071107674551286 2116 62-63 -0.030476786183534443 2116 64-65 -0.10302103434582932 2116 66-67 0.12697927198528447 2116 68-69 -0.1135685242501907 2116 70-71 0.3131950035314901 2116 72-73 -0.004272516023355877 2116 74-75 -0.6264826545180568 2116 76 -0.6633975287145084 2117 1 0.027553107039096858 2117 2 0.018040789230227006 2117 3 0.09616678921145905 2117 4 -0.03982752099510378 2117 5 0.050797122420028984 2117 6 0.005715205655263844 2117 7 0.07604471641283084 2117 8 -0.5521841116596562 2117 9 -0.2520112015019933 2117 10-11 -0.017682788382693104 2117 12-13 -0.26312919480018593 2117 14-15 -0.02547558826686469 2117 16-17 -0.137562383460903 2117 18-19 0.006834368750993747 2117 20-21 0.20055311366607498 2117 22-23 0.18411706437331787 2117 24-25 -0.294641446300659 2117 26-27 -0.1801822058177578 2117 28-29 -0.7124570963637993 2117 30-31 -0.089644986225899 2117 32-33 -0.15673728606296322 2117 34-35 -0.1527752939484941 2117 36-37 -0.04739745184510724 2117 38-39 -0.23875203603758877 2117 40-41 -0.29599327065264447 2117 42-43 0.13745141453406262 2117 44-45 -0.11487672212901856 2117 46-47 0.13702747481170974 2117 48-49 -0.13613640371740843 2117 50-51 -0.054679000897593255 2117 52-53 -0.04310676239480671 2117 54-55 0.06332252350005518 2117 56-57 -0.09224390918636516 2117 58-59 0.1300321367695787 2117 60-61 0.12412930530070554 2117 62-63 0.09344895806882647 2117 64-65 -0.14153472300608172 2117 66-67 0.027679158328524522 2117 68-69 0.0548628131109723 2117 70-71 -0.954865453887102 2117 72-73 -0.444483080119781 2117 74-75 -0.3565777672775212 2117 76 -1.2283513681195082 2118 1 0.1565003062158965 2118 2 -0.24686370623572174 2118 3 -0.08673886388520202 2118 4 0.08391033996058184 2118 5 0.04057138443045005 2118 6 0.053113752185588226 2118 7 0.10164121779556012 2118 8 0.12052795985619724 2118 9 0.03038638033606844 2118 10-11 0.2145507957706272 2118 12-13 0.26558647823345183 2118 14-15 0.08520677072727523 2118 16-17 0.1560577850386764 2118 18-19 0.020050652756403053 2118 20-21 0.03977106980472911 2118 22-23 0.009250513469716282 2118 24-25 0.05049328905209194 2118 26-27 0.15395503773487462 2118 28-29 0.26278246888156076 2118 30-31 0.36031964172445896 2118 32-33 -0.2527884790657282 2118 34-35 0.20715138943950961 2118 36-37 0.07193498110878238 2118 38-39 0.07515882639729199 2118 40-41 0.22706287443706685 2118 42-43 0.09600180362660637 2118 44-45 0.09150310937139494 2118 46-47 0.14654577180830586 2118 48-49 -1.4695101948802858E-4 2118 50-51 0.1492890999449017 2118 52-53 0.21446607187106537 2118 54-55 0.22494063398946906 2118 56-57 0.17249980423571998 2118 58-59 0.3309775351874791 2118 60-61 0.19368361747512353 2118 62-63 0.13583110483067884 2118 64-65 -0.039148717475178785 2118 66-67 0.027003873744305906 2118 68-69 -0.45996129133303754 2118 70-71 -0.02496642501065338 2118 72-73 0.4278881334630853 2118 74-75 0.6278585308767077 2118 76 0.37528873735351453 2119 1 0.14352287832980437 2119 2 0.09827847142997825 2119 3 0.12061296177032688 2119 4 0.10314071493239396 2119 5 0.09867253866297432 2119 6 0.17532299014609976 2119 7 0.1906585825371394 2119 8 0.09055481677646782 2119 9 0.22433024732255546 2119 10-11 -0.0050609064660847025 2119 12-13 0.0053101184737300855 2119 14-15 -0.3644518798662375 2119 16-17 0.038266612969877656 2119 18-19 0.296389911013371 2119 20-21 0.2962341569154603 2119 22-23 0.20556229976244111 2119 24-25 0.039319990161224894 2119 26-27 0.08421930162974434 2119 28-29 0.1519822966692459 2119 30-31 0.1305699646225449 2119 32-33 -0.053688493416750305 2119 34-35 0.2965377805664815 2119 36-37 0.21957628746218205 2119 38-39 -0.250127681588026 2119 40-41 -0.007135620755335026 2119 42-43 0.19806835138477652 2119 44-45 0.025160365865239953 2119 46-47 0.15356752386357897 2119 48-49 0.3262671375878483 2119 50-51 0.1429541469957698 2119 52-53 0.20315951345213534 2119 54-55 0.07624350214454267 2119 56-57 0.08613737932233789 2119 58-59 0.2923220213182347 2119 60-61 0.11601381408418376 2119 62-63 -0.09379556347610674 2119 64-65 -0.3169948334724708 2119 66-67 0.044009839670870576 2119 68-69 0.16590745828256104 2119 70-71 -0.0994687519270272 2119 72-73 -0.8339668391674522 2119 74-75 0.22941254198849492 2119 76 -0.5498604108105809 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 3.0 23 14.0 24 18.0 25 46.0 26 95.0 27 198.0 28 325.0 29 559.0 30 892.0 31 1251.0 32 2127.0 33 3663.0 34 5848.0 35 10347.0 36 13560.0 37 50610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.87515213774468 14.9312728206617 13.153633998459084 39.03994104313454 2 17.794253938832252 17.45592192681756 39.38943912815302 25.360385006197173 3 17.244883147046018 16.223187467199658 35.8073629085387 30.724566477215625 4 13.87719552910437 26.61545161182264 31.14106100025682 28.366291858816172 5 14.75149904530076 28.07262413881662 41.99448395993613 15.181392855946493 6 27.155889545205845 39.49216699978784 15.945152249405407 17.406791205600904 7 25.11919782931541 23.921078196009248 21.69902966825597 29.260694306419378 8 21.921234521031298 38.8914322721842 14.614156347354198 24.57317685943031 9 20.410464843619145 15.951851893207678 15.416996996326363 48.22068626684681 10-11 24.864053061178915 28.158602900945766 23.236039617227018 23.7413044206483 12-13 23.778152461560794 17.526826490391596 26.830398517145504 31.86462253090211 14-15 21.053072344986994 16.236586754804204 19.433991759438126 43.276349140770684 16-17 29.049655526647832 25.00474558102661 16.995879719061605 28.949719173263954 18-19 32.03323023325926 22.75701508536463 17.2716817222551 27.93807295912101 20-21 24.27392610292886 19.283808077537213 17.38780888149447 39.054456938039465 22-23 21.935192112286032 25.155487566577712 18.424578759895933 34.48474156124033 24-25 30.41694116596134 25.508893777147517 18.348091159820004 25.72607389707114 26-27 30.598389852272856 27.341246357068684 19.380952912670143 22.67941087798832 28-29 23.526357515325437 24.12988376118003 23.808300858671014 28.53545786482352 30-31 28.112822001630246 18.636734146967854 29.5141641636053 23.7362796877966 32-33 24.43471755418337 18.060006476322343 27.413825831593286 30.091450137901 34-35 21.164174771374654 31.04949920162578 23.577721451142846 24.208604575856718 36-37 29.660998023605078 28.93743649295979 19.684670098373104 21.716895385062028 38-39 22.40416717844501 22.380160121486874 27.940864477371953 27.274808222696162 40-41 21.072054669093426 17.15108813381422 29.65318177250243 32.123675424589926 42-43 28.22392442801791 16.56542760476568 28.440546244291344 26.770101722925066 44-45 20.71194881472135 24.64743124490548 31.83559074109227 22.805029199280906 46-47 25.844434270911265 19.533369809171813 27.61425684201123 27.00793907790569 48-49 25.863416595017696 17.559208102102573 22.457764328863185 34.119610974016545 50-51 22.51247808658173 18.045490581417422 28.361825429614658 31.080205902386187 52-53 17.055618209631852 18.06335629822348 39.137644181917665 25.743381310227004 54-55 17.830543676094553 19.50545462666235 32.71045256093884 29.953549136304254 56-57 24.332547986198733 20.747680248333463 24.568710430228794 30.35106133523901 58-59 19.36085398126333 21.361814263541657 32.95554786337193 26.321783891823085 60-61 24.580993110532955 27.144165168551872 25.341402682090735 22.933439038824435 62-63 18.477059303013725 38.60334758868654 19.401610147727148 23.517982960572596 64-65 17.004254273814443 41.08054088457631 18.408946257690634 23.506258583918623 66-67 17.019886776019742 40.50492982123117 18.70819701419208 23.766986388557008 68-69 16.804939870696874 41.13748785689561 18.652366649173153 23.405205623234366 70-71 17.30350503031589 38.8651920006253 19.438458188639636 24.392844780419175 72-73 17.46262157061983 34.460176200632 21.238987460500017 26.838214768248154 74-75 17.698225710999697 32.69482005873354 22.212110722779904 27.394843507486854 76 17.381109237692197 33.284947016983594 22.357827975479303 26.976115769844906 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 2.5 16 3.0 17 2.0 18 3.0 19 5.0 20 8.0 21 10.0 22 12.5 23 22.5 24 32.5 25 35.0 26 78.0 27 146.5 28 213.0 29 254.0 30 289.0 31 399.5 32 567.5 33 660.0 34 759.5 35 947.0 36 1144.5 37 1254.0 38 1568.5 39 4189.5 40 7688.0 41 8265.5 42 7651.0 43 6632.0 44 5023.5 45 4126.0 46 3818.0 47 3701.0 48 3325.5 49 2875.5 50 2684.0 51 2572.5 52 2169.5 53 1814.5 54 1751.0 55 1772.0 56 1763.0 57 1720.5 58 1708.0 59 1772.0 60 1952.5 61 2126.5 62 2184.0 63 2278.5 64 2428.5 65 2422.5 66 2397.5 67 2434.0 68 2376.5 69 2195.0 70 1837.0 71 1603.0 72 1415.0 73 1072.0 74 750.0 75 583.0 76 422.5 77 180.5 78 65.0 79 31.0 80 16.5 81 3.0 82 3.0 83 2.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 89557.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.36169143673862 #Duplication Level Percentage of deduplicated Percentage of total 1 63.41966825241008 28.134037540337438 2 20.49384580533112 18.18283327936398 3 7.830551989730424 10.42129593443282 4 3.4408114978982605 6.105608718469801 5 1.7065619572604396 3.785298748283216 6 0.896070880213446 2.3850731936085396 7 0.65695084195424 2.0400415377915744 8 0.37000679604319264 1.3131301852451511 9 0.2869440459110474 1.1456390901883717 >10 0.8406957134586825 4.940987304174994 >50 0.027687583377381763 0.8419219044854116 >100 0.01761937124015203 1.531985216119343 >500 0.0025170530343074328 0.6498654488203044 >1k 0.007551159102922299 8.019473631318602 >5k 0.0025170530343074328 10.502808267360452 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 9406 10.502808267360452 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 2560 2.8585146889690365 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 2503 2.79486807284746 No Hit CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG 2119 2.366090869502105 RNA PCR Primer, Index 47 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 582 0.6498654488203044 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT 312 0.3483814777181013 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 298 0.3327489755128019 Illumina PCR Primer Index 9 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT 190 0.21215538707192066 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 176 0.19652288486662126 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 168 0.18759002646359302 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 124 0.1384593052469377 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG 104 0.1161271592393671 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00446642920151412 0.0 2 0.0 0.0 0.0 0.00781625110264971 0.0 3 0.0 0.0 0.0 0.00781625110264971 0.0 4 0.0 0.0 0.0 0.05136393581741237 0.0 5 0.0 0.0 0.0 0.059180186920062085 0.0 6 0.0 0.0 0.0 0.06253000882119768 0.0 7 0.0 0.0 0.0 0.06476322342195473 0.0 8 0.0 0.0 0.0 0.0669964380227118 0.0 9 0.0 0.0 0.0 0.0748126891253615 0.0 10 0.0 0.0 0.0 0.0748126891253615 0.0 11 0.0 0.0 0.0 0.0748126891253615 0.0 12 0.0 0.0 0.0 0.07704590372611857 0.0 13 0.0 0.0 0.0 0.07927911832687562 0.0 14 0.0 0.0 0.0 0.0859787621291468 0.0 15 0.0 0.0 0.0 0.08821197672990386 0.0 16 0.0 0.0 0.0 0.08821197672990386 0.0 17 0.0 0.0 0.0 0.08821197672990386 0.0 18 0.0 0.0 0.0 0.1004946570340677 0.0 19 0.0 0.0 0.0 0.1161271592393671 0.0 20 0.0 0.0 0.0 0.12059358844088122 0.0 21 0.0 0.0 0.0 0.1272932322431524 0.0 22 0.0 0.0 0.0 0.13175966144466653 0.0 23 0.0 0.0 0.0 0.13622609064618063 0.0 24 0.0 0.0 0.0 0.14850877095034448 0.0 25 0.0 0.0 0.0 0.15632502205299417 0.0 26 0.0 0.0 0.0 0.17865716806056478 0.0 27 0.0 0.0 0.0 0.30148397110220304 0.0 28 0.0 0.0 0.0 0.43994327634914077 0.0 29 0.0 0.0 0.0 0.562770079390779 0.0 30 0.0 0.0 0.0 0.6788972386301462 0.0 31 0.0 0.0 0.0 0.8486215482876827 0.0 32 0.0 0.0 0.0 1.0630101499603604 0.0 33 0.0 0.0 0.0 1.3577944772602923 0.0 34 0.0 0.0 0.0 1.7072925622787722 0.0 35 0.0 0.0 0.0 2.185200486840783 0.0 36 0.0 0.0 0.0 2.8920129079803925 0.0 37 0.0 0.0 0.0 3.7272351686635328 0.0 38 0.0 0.0 0.0 4.641736547673549 0.0 39 0.0 0.0 0.0 5.530555958774858 0.0 40 0.0 0.0 0.0 6.2730998135265805 0.0 41 0.0 0.0 0.0 7.136237256719184 0.0 42 0.0 0.0 0.0 8.095402927744342 0.0 43 0.0 0.0 0.0 9.0076710921536 0.0 44 0.0 0.0 0.0 9.879741393749232 0.0 45 0.0 0.0 0.0 10.79312616545887 0.0 46 0.0 0.0 0.0 11.740009156179863 0.0 47 0.0 0.0 0.0 12.621012316178524 0.0 48 0.0 0.0 0.0 13.462934220663934 0.0 49 0.0 0.0 0.0 14.121732527887268 0.0 50 0.0 0.0 0.0 14.85534352423596 0.0 51 0.0 0.0 0.0 15.549873265071406 0.0 52 0.0 0.0 0.0 16.23100371830231 0.0 53 0.0 0.0 0.0 16.8607702357158 0.0 54 0.0 0.0 0.0 17.496119789631184 0.0 55 0.0 0.0 0.0 18.128119521645434 0.0 56 0.0 0.0 0.0 18.810366582176716 0.0 57 0.0 0.0 0.0 19.3988186294762 0.0 58 0.0 0.0 0.0 20.007369608182497 0.0 59 0.0 0.0 0.0 20.64606898399902 0.0 60 0.0 0.0 0.0 21.324966222629165 0.0 61 0.0 0.0 0.0 21.88103665821767 0.0 62 0.0 0.0 0.0 22.51303639023192 0.0 63 0.0 0.0 0.0 23.055707538215884 0.0 64 0.0 0.0 0.0 23.627410476009693 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTTAT 95 0.0 66.315796 1 CTTTATA 110 0.0 57.27273 2 TCTAGCA 20 0.006931226 52.500004 3 AGGCATA 20 0.006931226 52.500004 39 TCGCTAA 20 0.006931226 52.500004 14 CCGAGAG 20 0.006931226 52.500004 29 CTCGCTA 20 0.006931226 52.500004 13 TGCAGAA 20 0.006931226 52.500004 18 TGTAAAG 20 0.006931226 52.500004 48 TTTATAC 125 0.0 50.4 3 AACTCGT 35 0.0012495791 40.000004 37 TGCTTGA 2105 0.0 36.579575 55 CTTGAAA 2080 0.0 36.346153 57 GCTTGAA 2100 0.0 36.333332 56 CTGCTTG 2110 0.0 36.327015 54 TTCTGCT 2110 0.0 36.327015 52 TCTGCTT 2110 0.0 36.327015 53 TTGAAAA 2095 0.0 36.252983 58 GTCTTCT 2130 0.0 36.150234 49 CCGTCTT 2125 0.0 36.070587 47 >>END_MODULE