FastQCFastQC Report
Wed 25 May 2016
SRR1780293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376373
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGC250.002344537335.21895237
TAGCAAC250.002364508335.1581236
GAATACT300.00573241429.34131624
TTATCAG300.005762329.31011831
TAATTCT300.005762329.31011841
AGCATTT300.00577353929.2984391
CTTTAGT300.00577353929.2984391
GTTACTA609.6891035E-625.68048735
GAACTAA609.819982E-625.6361331
TATCTTC450.001382362724.48693518
TAGACAC450.001406144224.4153633
TATACCG450.001406144224.4153636
GGACTCA500.00259504121.9738276
CATGGCA654.9056305E-420.31862437
TTATATC550.004433171420.03476515
TTTTTAG550.004491617419.98948728
AGACACA550.004508927519.9762084
GAAAATT1051.7950115E-618.84722926
GTACTGA600.00745385818.3115236
AAATTTG851.433277E-418.10812228
Produced by FastQC (version 0.11.5)