FastQCFastQC Report
Wed 25 May 2016
SRR1780293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376373
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT197295.241874417134067No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC56661.5054214834751696No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG51741.374700098041039No Hit
CACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTG30950.8223225364199875RNA PCR Primer, Index 14 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC11430.3036880966488032No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT4570.12142209988495456RNA PCR Primer, Index 35 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT1700.037.616761
TTCTGTT301.2999635E-436.6641238
TCTTCTG3600.036.0530544
CTTATAT250.002324076835.281931
GATGTAT250.002351542535.19755624
TGAAGTT250.002351542535.19755639
GAAGTGT250.002351542535.19755637
ATAAATC250.002351542535.19755638
ATGCCGT24750.034.75314344
GCTTATA401.8159713E-533.0020943
CGTCTTC3950.032.8584842
GTCTTCT3950.032.8584843
CCGTCTT4000.032.44774641
CTTTATA2000.031.9020212
ACTCGCA353.214994E-431.42639221
TTTATAC2500.029.9218963
ATGTAGG300.00573827329.3351963
CATTAGT300.005742000429.33129713
ACGATTC300.005742000429.33129714
TCTGTTG300.005742000429.33129739