Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780293_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 376373 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 61 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT | 19729 | 5.241874417134067 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC | 5666 | 1.5054214834751696 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG | 5174 | 1.374700098041039 | No Hit |
CACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTG | 3095 | 0.8223225364199875 | RNA PCR Primer, Index 14 (96% over 25bp) |
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC | 1143 | 0.3036880966488032 | No Hit |
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT | 457 | 0.12142209988495456 | RNA PCR Primer, Index 35 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCTTTAT | 170 | 0.0 | 37.61676 | 1 |
TTCTGTT | 30 | 1.2999635E-4 | 36.66412 | 38 |
TCTTCTG | 360 | 0.0 | 36.05305 | 44 |
CTTATAT | 25 | 0.0023240768 | 35.28193 | 1 |
GATGTAT | 25 | 0.0023515425 | 35.197556 | 24 |
TGAAGTT | 25 | 0.0023515425 | 35.197556 | 39 |
GAAGTGT | 25 | 0.0023515425 | 35.197556 | 37 |
ATAAATC | 25 | 0.0023515425 | 35.197556 | 38 |
ATGCCGT | 2475 | 0.0 | 34.753143 | 44 |
GCTTATA | 40 | 1.8159713E-5 | 33.002094 | 3 |
CGTCTTC | 395 | 0.0 | 32.85848 | 42 |
GTCTTCT | 395 | 0.0 | 32.85848 | 43 |
CCGTCTT | 400 | 0.0 | 32.447746 | 41 |
CTTTATA | 200 | 0.0 | 31.902021 | 2 |
ACTCGCA | 35 | 3.214994E-4 | 31.426392 | 21 |
TTTATAC | 250 | 0.0 | 29.921896 | 3 |
ATGTAGG | 30 | 0.005738273 | 29.335196 | 3 |
CATTAGT | 30 | 0.0057420004 | 29.331297 | 13 |
ACGATTC | 30 | 0.0057420004 | 29.331297 | 14 |
TCTGTTG | 30 | 0.0057420004 | 29.331297 | 39 |