FastQCFastQC Report
Wed 25 May 2016
SRR1780292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167918
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAG150.00222158870.014
GGTTAAG200.006941753452.51
CGACAGC352.0797948E-550.036
GAAAACG352.0797948E-550.013
CAAGATG352.0797948E-550.017
CGATTTC451.677141E-646.66666442
AAAACGT404.577979E-543.7514
TGAATTG350.00125270940.032
GCGACAG459.168369E-538.88888535
AAACGTA459.168369E-538.88888515
GATAGTC459.168369E-538.88888552
TACTAGA556.66511E-638.181821
GCAAATT400.002413573735.038
GATTTTC400.002413573735.021
CCTACAG400.002413573735.02
GTCAGTA501.7042822E-435.056
TGTGAAA501.7042822E-435.010
TTGCGTT400.002413573735.033
AGCGATT501.7042822E-435.040
AAATTCG400.002413573735.040
Produced by FastQC (version 0.11.5)