##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780292_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 167918 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.674198120511203 34.0 32.0 34.0 21.0 34.0 2 30.593825557712695 34.0 32.0 34.0 21.0 34.0 3 30.542526709465335 34.0 32.0 34.0 21.0 34.0 4 30.345120832787433 34.0 32.0 34.0 11.0 34.0 5 30.30809680915685 34.0 31.0 34.0 11.0 34.0 6 33.59352183804 37.0 34.0 38.0 21.0 38.0 7 33.89884348312867 38.0 34.0 38.0 21.0 38.0 8 33.89568122536 38.0 34.0 38.0 21.0 38.0 9 34.14653580914494 38.0 35.0 38.0 21.0 38.0 10-11 33.90509951285746 38.0 34.5 38.0 21.0 38.0 12-13 33.977762955728394 38.0 34.0 38.0 21.0 38.0 14-15 33.90984289951048 38.0 34.0 38.0 21.0 38.0 16-17 33.99457473290535 38.0 35.0 38.0 21.0 38.0 18-19 33.99672161412118 38.0 34.5 38.0 21.0 38.0 20-21 34.00940935456592 38.0 34.5 38.0 21.0 38.0 22-23 33.99426505794495 38.0 35.0 38.0 21.0 38.0 24-25 34.01999190080873 38.0 35.0 38.0 21.0 38.0 26-27 34.02431543967889 38.0 35.0 38.0 21.0 38.0 28-29 34.115922652723356 38.0 35.0 38.0 21.0 38.0 30-31 34.14025595826534 38.0 35.5 38.0 21.0 38.0 32-33 34.20439142914994 38.0 36.0 38.0 21.5 38.0 34-35 34.16416346073679 38.0 35.5 38.0 21.5 38.0 36-37 34.09523100560988 38.0 35.0 38.0 21.5 38.0 38-39 34.09689848616587 38.0 35.0 38.0 21.0 38.0 40-41 34.168638859443305 38.0 35.5 38.0 21.5 38.0 42-43 34.24340451887231 38.0 36.0 38.0 22.0 38.0 44-45 34.31046701366143 38.0 36.0 38.0 23.0 38.0 46-47 34.26032646887171 38.0 36.0 38.0 22.5 38.0 48-49 34.18793696923498 38.0 36.0 38.0 21.5 38.0 50-51 34.15870841720364 38.0 35.5 38.0 21.5 38.0 52-53 34.20039245345943 38.0 36.0 38.0 22.0 38.0 54-55 34.188961278719376 38.0 36.0 38.0 22.0 38.0 56-57 34.14893281244417 38.0 36.0 38.0 22.0 38.0 58-59 34.21029907454829 38.0 36.0 38.0 22.0 38.0 60-61 34.21320823258972 38.0 36.0 38.0 22.0 38.0 62-63 34.11134899176979 38.0 35.5 38.0 22.0 38.0 64-65 34.07955668838362 38.0 35.5 38.0 21.5 38.0 66-67 33.968332757655524 38.0 35.0 38.0 22.0 38.0 68-69 33.91836789385295 38.0 35.0 38.0 21.0 38.0 70-71 33.94294536619064 38.0 35.0 38.0 21.5 38.0 72-73 33.95674376779142 38.0 35.0 38.0 21.5 38.0 74-75 33.961159613620936 38.0 35.0 38.0 21.0 38.0 76 32.346937195535915 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.11346843773500481 1101 2 0.11428877533133885 1101 3 0.4302335650418776 1101 4 0.479730192809825 1101 5 -0.6305301585292611 1101 6 -0.19644082393283924 1101 7 -0.3533830412362704 1101 8 -0.4395634269192854 1101 9 -0.167895034919745 1101 10-11 -0.48656002862646375 1101 12-13 -0.13641644634037675 1101 14-15 -0.2119107230199262 1101 16-17 -0.5377710784736109 1101 18-19 -0.1226368446470758 1101 20-21 0.06855923796801733 1101 22-23 -0.23726526226130318 1101 24-25 -0.32359305013900297 1101 26-27 -0.574738511527876 1101 28-29 -0.23546658048136493 1101 30-31 -0.23180951534177296 1101 32-33 0.01151620535078024 1101 34-35 -0.1969283329985032 1101 36-37 -0.40726810009689984 1101 38-39 -0.12280019510639306 1101 40-41 0.0136327802360654 1101 42-43 -0.4711599795814365 1101 44-45 -0.12331696811278903 1101 46-47 0.48664160949874713 1101 48-49 0.14694109060152272 1101 50-51 0.09061410454484076 1101 52-53 -0.07000032641442289 1101 54-55 -0.20414657904531452 1101 56-57 0.1275277459594477 1101 58-59 0.12183098800289827 1101 60-61 0.2938397927919496 1101 62-63 0.12607386047437075 1101 64-65 0.01801061635750756 1101 66-67 -0.14392990255715432 1101 68-69 -0.14185424327448004 1101 70-71 1.7493153037406728E-4 1101 72-73 -0.053840061457357535 1101 74-75 0.10124477716065883 1101 76 -0.5671008240137425 1102 1 0.19710189389792276 1102 2 -0.09302190729135162 1102 3 0.32185130489023095 1102 4 0.582877550761637 1102 5 -0.16771988764045176 1102 6 0.17737076236551275 1102 7 -0.2927726056539086 1102 8 -0.05947423573573474 1102 9 0.2590997355252469 1102 10-11 0.3167407937718636 1102 12-13 0.39330860098228015 1102 14-15 0.2495787911340983 1102 16-17 0.259244118249633 1102 18-19 0.4358480816151342 1102 20-21 0.13348218491317 1102 22-23 -0.29138955242139986 1102 24-25 0.18431544868995786 1102 26-27 0.15690457401652225 1102 28-29 0.32268949339545827 1102 30-31 0.060518866490603784 1102 32-33 -0.015291948049501514 1102 34-35 -0.008191126158372697 1102 36-37 0.1027525400690763 1102 38-39 0.4100823698352727 1102 40-41 0.27370956593125584 1102 42-43 0.10277298800388479 1102 44-45 0.11666773891449367 1102 46-47 -0.24859478991520234 1102 48-49 -0.11748028961599744 1102 50-51 -0.1940143189956416 1102 52-53 0.06522484389368088 1102 54-55 -0.3722409463119405 1102 56-57 -0.03590119518933932 1102 58-59 -0.047830106888390844 1102 60-61 0.17146927507669574 1102 62-63 -0.08041870157980213 1102 64-65 0.014492163193573049 1102 66-67 0.32350836409667494 1102 68-69 0.2848678203143393 1102 70-71 0.2726545319880955 1102 72-73 0.5109144489001594 1102 74-75 -0.16802383374547247 1102 76 0.05335539851505189 1103 1 0.00280913638551894 1103 2 -0.24927158224792478 1103 3 -0.04815732826315866 1103 4 0.28151808913756327 1103 5 0.37615837449536826 1103 6 -0.2429389910175388 1103 7 -0.0012060523623915742 1103 8 -0.08474961252607471 1103 9 -0.17590198070404028 1103 10-11 -0.09688866712411226 1103 12-13 0.21177314784574008 1103 14-15 0.03898965642569863 1103 16-17 -0.07787012021262996 1103 18-19 -0.40098271897982585 1103 20-21 -0.1217263109418738 1103 22-23 -0.07264052755166972 1103 24-25 -0.3434978671690345 1103 26-27 -0.1084062715124432 1103 28-29 0.10478038073800633 1103 30-31 -0.3611586696282174 1103 32-33 0.220468549552983 1103 34-35 0.15339966237097968 1103 36-37 -0.030153816220121143 1103 38-39 -0.5075516260665793 1103 40-41 -0.053879599981307535 1103 42-43 -0.09041202151338723 1103 44-45 0.05507070955137294 1103 46-47 0.2800688395456916 1103 48-49 0.023219307850219195 1103 50-51 -0.36634454231347746 1103 52-53 0.18691330147161977 1103 54-55 0.19258311418254692 1103 56-57 0.08670518502305669 1103 58-59 0.04415718261348189 1103 60-61 0.035559517533087615 1103 62-63 -0.2536772489217327 1103 64-65 0.11073362105233997 1103 66-67 0.06865611583631193 1103 68-69 -0.06151145595696761 1103 70-71 0.07357193455307254 1103 72-73 -0.008419134066521394 1103 74-75 -0.18432021094147188 1103 76 0.5634863520104361 1104 1 0.005194852870047839 1104 2 0.009504263013990766 1104 3 -0.5536932166218875 1104 4 0.292928483678633 1104 5 -0.02909533234368311 1104 6 0.2348404969512714 1104 7 0.248268063896397 1104 8 -0.11694908920762259 1104 9 0.12917728665012618 1104 10-11 0.122399722388991 1104 12-13 -0.5548370825169897 1104 14-15 0.08649619790638496 1104 16-17 -0.13078685101145737 1104 18-19 0.1183005399601953 1104 20-21 -0.27283708001478857 1104 22-23 -0.39110289479809524 1104 24-25 -0.17614883428205985 1104 26-27 -0.08188890452255038 1104 28-29 -0.29828770196682797 1104 30-31 -0.12224840335147746 1104 32-33 -0.39074944849740234 1104 34-35 -0.1703497604395494 1104 36-37 -0.28201621424998535 1104 38-39 -0.024654391445473323 1104 40-41 -0.36695486548846645 1104 42-43 0.058256594771044945 1104 44-45 0.0074025550185155 1104 46-47 -0.38303977468650885 1104 48-49 -0.21806128319180118 1104 50-51 -0.29202819117705303 1104 52-53 0.22073660453457222 1104 54-55 -0.2820333754793509 1104 56-57 -0.23974291923018853 1104 58-59 -0.15399324578937268 1104 60-61 -0.10455130284554315 1104 62-63 -0.02351204446839006 1104 64-65 -0.2909517722060855 1104 66-67 0.04317512460958994 1104 68-69 0.27539532153007684 1104 70-71 -0.06624644315971295 1104 72-73 -0.05356048135501368 1104 74-75 1.264567513103998E-4 1104 76 0.10139102596252059 1105 1 -0.11547384767810698 1105 2 -0.14793800699618131 1105 3 -0.04483095008419724 1105 4 0.32225110192900175 1105 5 0.4060957781060033 1105 6 0.3944555476742906 1105 7 0.26818522043085835 1105 8 0.16345867922753143 1105 9 0.09669471106166583 1105 10-11 0.14759256907897367 1105 12-13 -0.9120437199156015 1105 14-15 -0.02562523970058095 1105 16-17 -0.027324291793263455 1105 18-19 0.17414050821180638 1105 20-21 0.15762897648743746 1105 22-23 -0.10456619811927226 1105 24-25 -0.08378333089639511 1105 26-27 -0.004494753172004096 1105 28-29 -0.3114856085571063 1105 30-31 0.017972843269056682 1105 32-33 -0.3313700303859335 1105 34-35 0.03513179820816248 1105 36-37 -0.2802898953274422 1105 38-39 0.06114564327933181 1105 40-41 0.06350374998391572 1105 42-43 -0.10448864917071177 1105 44-45 0.034356445436941385 1105 46-47 -0.09907023330198683 1105 48-49 0.1479282497595591 1105 50-51 -0.04229482807960494 1105 52-53 -0.012465270604955947 1105 54-55 0.11230400081353054 1105 56-57 -0.2275619533845159 1105 58-59 -0.22899138553191278 1105 60-61 -0.2890193932292533 1105 62-63 -0.03975333226262734 1105 64-65 -0.10615379616620402 1105 66-67 -0.1361958603347304 1105 68-69 -0.17098709779676113 1105 70-71 -0.1759669084721125 1105 72-73 0.18542555431232444 1105 74-75 0.0017982081217056134 1105 76 0.1855341913766111 1106 1 -0.29025346422803366 1106 2 0.21129802967484324 1106 3 0.16168584115234808 1106 4 0.3322020081809889 1106 5 0.07479863611591853 1106 6 0.3107367574460511 1106 7 -0.48229323558789616 1106 8 -0.3502782703033134 1106 9 -0.07267627223275497 1106 10-11 -0.269652894766395 1106 12-13 0.18743352711454264 1106 14-15 0.23484817478672682 1106 16-17 0.3033071722576821 1106 18-19 0.24046601493466113 1106 20-21 0.34384993261632246 1106 22-23 -0.02031388660158484 1106 24-25 0.27674735147243723 1106 26-27 0.3752500376533945 1106 28-29 0.483067090130632 1106 30-31 -0.33562940437293065 1106 32-33 0.3358007894258108 1106 34-35 0.16848030508275968 1106 36-37 0.2081977920780247 1106 38-39 0.24508077883778867 1106 40-41 0.13692166357304103 1106 42-43 -0.034964462056471746 1106 44-45 0.1294260234656548 1106 46-47 0.15017231330770642 1106 48-49 0.0776037807111365 1106 50-51 0.11145719709868729 1106 52-53 0.21357399460407578 1106 54-55 -0.2784832122964147 1106 56-57 -0.0013538213453401227 1106 58-59 0.11739119328484549 1106 60-61 0.17666148272459736 1106 62-63 -0.05459403455284928 1106 64-65 0.27315047249357605 1106 66-67 0.23149820191594728 1106 68-69 0.19078683601723867 1106 70-71 0.2524286608538375 1106 72-73 0.22403487478241146 1106 74-75 -0.1122505332085737 1106 76 0.33661861010761385 1107 1 -0.37604839173191706 1107 2 -0.5773645000254994 1107 3 -0.7888640182205613 1107 4 -0.5872263563284044 1107 5 -0.5720810297521233 1107 6 -1.440603788296201 1107 7 -0.5828155647892217 1107 8 -0.44523504049335116 1107 9 -0.38688233680713324 1107 10-11 -0.5292152890480537 1107 12-13 -0.153447041544716 1107 14-15 -0.482178864306114 1107 16-17 -0.4560840885249675 1107 18-19 -0.2425520855331058 1107 20-21 -0.1825120668181981 1107 22-23 0.17394247634032922 1107 24-25 -0.02606965473300704 1107 26-27 -0.07206812680342267 1107 28-29 -0.38077016935239527 1107 30-31 -0.4751453693922443 1107 32-33 -0.16205880173873766 1107 34-35 -0.4449117592832792 1107 36-37 -0.06345803613370293 1107 38-39 -0.21656299562313563 1107 40-41 -0.16841026100916423 1107 42-43 0.022057715852916715 1107 44-45 -0.2942137132367719 1107 46-47 -0.34688865486145914 1107 48-49 -0.07052590375838008 1107 50-51 -0.06684406730505543 1107 52-53 -0.43342472065457827 1107 54-55 -0.30544789927092353 1107 56-57 -0.0663547886216378 1107 58-59 -0.48479534718946127 1107 60-61 -0.2944640796597682 1107 62-63 -0.4291928629084296 1107 64-65 -0.2041433364420655 1107 66-67 -0.29058049965203026 1107 68-69 -0.2090628641271124 1107 70-71 -0.2506443015414348 1107 72-73 -0.21001424301200444 1107 74-75 -0.10515469348873552 1107 76 -0.17818567765460358 1108 1 -0.33280730197064656 1108 2 -0.23100674206527927 1108 3 -0.21254088990699316 1108 4 -0.14085634008618086 1108 5 0.1798113425318988 1108 6 0.3392527898043838 1108 7 0.18372545448692534 1108 8 -0.1189961878685466 1108 9 -0.4224773231697938 1108 10-11 0.3608738899535098 1108 12-13 0.49145351314254526 1108 14-15 0.10405814957638171 1108 16-17 0.09792896654536065 1108 18-19 0.11271591115716006 1108 20-21 0.18763441965172944 1108 22-23 0.5095512532702458 1108 24-25 -0.05126042424666366 1108 26-27 -0.12552955918366848 1108 28-29 -0.07558491606565099 1108 30-31 0.24614726644484364 1108 32-33 0.2113361294616567 1108 34-35 0.019838018535359936 1108 36-37 -0.22650084818359062 1108 38-39 0.1556903830658456 1108 40-41 0.3022847835803333 1108 42-43 0.04200806981081229 1108 44-45 0.13726249037328841 1108 46-47 0.3451373326963818 1108 48-49 -0.23363000721827376 1108 50-51 0.11653217001529015 1108 52-53 0.11271238822960328 1108 54-55 -0.05170912326038035 1108 56-57 0.0045134042011412134 1108 58-59 0.2850247625221556 1108 60-61 0.10976043077510411 1108 62-63 -0.12696492015460592 1108 64-65 -0.19748555702985726 1108 66-67 -0.10029365585318573 1108 68-69 -0.25215072536335725 1108 70-71 0.29389156925627447 1108 72-73 7.132860247978101E-4 1108 74-75 -0.046192357060192535 1108 76 -0.548385794108281 1109 1 0.5719292266648175 1109 2 0.36264235984375404 1109 3 0.541239033397396 1109 4 0.4021203691078874 1109 5 0.7646310451535534 1109 6 0.4378024695427385 1109 7 0.4495896384728013 1109 8 0.5397449174259634 1109 9 0.7761214668367913 1109 10-11 0.7198542771931997 1109 12-13 0.19531075899691075 1109 14-15 0.6066129284234449 1109 16-17 0.43473136563420667 1109 18-19 0.45744125936985824 1109 20-21 0.385055085670551 1109 22-23 0.4640432754639079 1109 24-25 0.2795912731922954 1109 26-27 0.33788329098820924 1109 28-29 0.12271607763361203 1109 30-31 0.3033346064446292 1109 32-33 0.3131432828424252 1109 34-35 0.9427322851727737 1109 36-37 0.5594436885602931 1109 38-39 0.48823481740959096 1109 40-41 0.3950640068021798 1109 42-43 0.6068231748754371 1109 44-45 0.10531991804013785 1109 46-47 0.4679025542672832 1109 48-49 0.22062691262750178 1109 50-51 0.5806738374656248 1109 52-53 0.3217444979858328 1109 54-55 0.47509515664724944 1109 56-57 0.26936926033939557 1109 58-59 0.3756959013579291 1109 60-61 0.3544053217398542 1109 62-63 0.5139188556935892 1109 64-65 0.14448648146424148 1109 66-67 0.2005093492772616 1109 68-69 0.46123339007711905 1109 70-71 0.6436010872442139 1109 72-73 0.20671257549326327 1109 74-75 0.4938168380538599 1109 76 -0.26500909745300305 1110 1 0.2569709399361031 1110 2 -0.03683019559436573 1110 3 0.3542424915455946 1110 4 -0.3432029215715282 1110 5 0.3131528663765266 1110 6 0.15869296835752777 1110 7 -0.3554251894920242 1110 8 -0.2713704123295031 1110 9 0.005098080724792453 1110 10-11 0.06828323123163926 1110 12-13 0.20292227658010376 1110 14-15 0.18156598175281147 1110 16-17 -0.046602843352872014 1110 18-19 -0.10513121294955852 1110 20-21 0.07175404206915204 1110 22-23 0.022098092756692722 1110 24-25 0.05713075457267536 1110 26-27 0.07942262870665928 1110 28-29 0.11665662211237304 1110 30-31 0.16110928294872195 1110 32-33 0.2586056177594642 1110 34-35 0.13432600919817617 1110 36-37 0.14084870236209923 1110 38-39 0.3236828887480101 1110 40-41 0.14341445985037637 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-0.21802868442915013 2116 7 -0.3203515187681276 2116 8 0.08480151271854197 2116 9 -0.5025592703322204 2116 10-11 0.28666788049493164 2116 12-13 0.2629799319292445 2116 14-15 0.07802157912628616 2116 16-17 -0.16954086385255351 2116 18-19 -0.028913043506904046 2116 20-21 -0.13318770200515218 2116 22-23 0.06952862606867427 2116 24-25 0.03842279493142087 2116 26-27 -0.17935579858353634 2116 28-29 -0.3168245197838502 2116 30-31 -0.06838102774955246 2116 32-33 -0.09200596936417327 2116 34-35 0.3467399265588469 2116 36-37 -0.5112723338326504 2116 38-39 -0.06020063943905285 2116 40-41 -0.03911076436487093 2116 42-43 0.1407982279973723 2116 44-45 -0.12593161432338462 2116 46-47 -0.23071985093049818 2116 48-49 -0.2293950512495826 2116 50-51 -0.08726043435522968 2116 52-53 -0.25998921809785003 2116 54-55 -0.0029026571807975188 2116 56-57 -0.2765582282189527 2116 58-59 -0.14518255156785642 2116 60-61 -0.19514620653735903 2116 62-63 0.40280717704042956 2116 64-65 -0.08346280751909063 2116 66-67 -0.008796183576613714 2116 68-69 -0.28895560142337473 2116 70-71 -0.10394457718861361 2116 72-73 -0.06003871169209418 2116 74-75 -0.10945169332894267 2116 76 -0.43534151817873123 2117 1 0.0389027180385888 2117 2 -0.12757438867140536 2117 3 0.23731801933316987 2117 4 0.1895576971617814 2117 5 -0.21561218627626744 2117 6 0.14944723852430286 2117 7 0.49995345715482387 2117 8 0.4538374276463131 2117 9 0.08648065743663835 2117 10-11 0.3021750032878643 2117 12-13 0.13167977141559817 2117 14-15 0.1876197934120043 2117 16-17 0.1366288793256203 2117 18-19 -0.0536095723993526 2117 20-21 -0.32901685529568425 2117 22-23 0.31339148722437926 2117 24-25 0.20250285111119126 2117 26-27 0.40350435390441675 2117 28-29 0.2893527474713693 2117 30-31 0.3103358663114406 2117 32-33 -0.019962931643469517 2117 34-35 0.07571765529078789 2117 36-37 -0.005611719867957277 2117 38-39 0.02028942877925033 2117 40-41 0.013944010916027594 2117 42-43 0.2256723251081283 2117 44-45 0.20178457749042167 2117 46-47 0.46302764104382277 2117 48-49 0.2886802898419063 2117 50-51 0.5371852919690099 2117 52-53 0.15893885172557987 2117 54-55 0.3999890964307653 2117 56-57 0.4018301144776757 2117 58-59 0.004433720502767358 2117 60-61 -0.0011805163286950915 2117 62-63 0.02817334879003397 2117 64-65 -0.012300086812828681 2117 66-67 -0.062442852918501046 2117 68-69 -0.023481082964302402 2117 70-71 0.10341647818859911 2117 72-73 -1.2322121667482762 2117 74-75 -0.15942410504644045 2117 76 -0.2424291987565823 2118 1 -0.32967260399879805 2118 2 -0.6167626744811905 2118 3 -0.28942458216324596 2118 4 -0.8381542304344443 2118 5 -0.46420190679152995 2118 6 -0.01528677530851752 2118 7 -0.0607078443647282 2118 8 -0.18565554023994224 2118 9 -0.5854345972364356 2118 10-11 -1.3831205511820883 2118 12-13 -0.75663432109463 2118 14-15 -0.502082723750469 2118 16-17 -0.573277787683871 2118 18-19 -0.6718493584791077 2118 20-21 -0.7385147083797889 2118 22-23 -0.5004876169846142 2118 24-25 -0.1826512897367678 2118 26-27 -0.7410640774328456 2118 28-29 -0.32694067293847695 2118 30-31 -0.45867912353248386 2118 32-33 -0.5175203327182132 2118 34-35 -0.632782588969107 2118 36-37 -0.7323183251696861 2118 38-39 -0.31039218487873654 2118 40-41 -0.44704125635571756 2118 42-43 -0.1506138960627723 2118 44-45 -0.376373445804262 2118 46-47 -0.7449120910416411 2118 48-49 -0.27479642833360174 2118 50-51 -0.1195549954863182 2118 52-53 -0.39450264160226567 2118 54-55 -0.4585432872886699 2118 56-57 -0.5006536229557952 2118 58-59 -0.3645786981844026 2118 60-61 -0.1524596668184941 2118 62-63 -0.6920308010569869 2118 64-65 -0.6163830837531563 2118 66-67 -0.26630987269069806 2118 68-69 -0.285906198023568 2118 70-71 -0.1287101255704357 2118 72-73 -0.5221203764518165 2118 74-75 -0.5565264113740582 2118 76 -0.17347591667497397 2119 1 0.14122108704565406 2119 2 -0.20543803196796162 2119 3 -0.1958153377261631 2119 4 0.15976970590650552 2119 5 0.46428002990147377 2119 6 -0.13193463142332718 2119 7 0.548588364877638 2119 8 0.44779238214323414 2119 9 0.07718176069557359 2119 10-11 0.24323199652595662 2119 12-13 0.3504072105329854 2119 14-15 0.0679365354934518 2119 16-17 0.27727151494512015 2119 18-19 0.10122290510331311 2119 20-21 -0.05917853102906889 2119 22-23 -0.09667511504315485 2119 24-25 0.252294401266262 2119 26-27 0.13002324149402256 2119 28-29 0.10398490975720165 2119 30-31 -0.10558004197061877 2119 32-33 -0.020471345899402138 2119 34-35 -0.17522799522524934 2119 36-37 0.07365514677498197 2119 38-39 -0.47618217628455994 2119 40-41 -0.12685114898025773 2119 42-43 -0.13852768506015423 2119 44-45 -0.0836976534313294 2119 46-47 -0.09625091912700157 2119 48-49 0.3907114047943736 2119 50-51 0.009474172949232695 2119 52-53 -0.07368609107781054 2119 54-55 -0.27762288180910843 2119 56-57 -0.17188486849044438 2119 58-59 -0.015864718665469013 2119 60-61 -0.2842198167506709 2119 62-63 -0.2259803956809634 2119 64-65 -0.21505823415127878 2119 66-67 -0.28763223722884135 2119 68-69 -0.497615507693574 2119 70-71 -0.23505014441549577 2119 72-73 0.2143179059549638 2119 74-75 -0.006454965791782286 2119 76 -0.3319660333694259 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 15.0 13 188.0 14 1033.0 15 1845.0 16 2485.0 17 2942.0 18 2872.0 19 2618.0 20 2074.0 21 1582.0 22 1203.0 23 1097.0 24 1010.0 25 1144.0 26 1289.0 27 1532.0 28 1808.0 29 2209.0 30 2762.0 31 3389.0 32 4506.0 33 5680.0 34 8090.0 35 12164.0 36 22849.0 37 79532.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.22645577007825 17.80809680915685 10.029895544253744 28.935551876511155 2 22.768851463214187 19.15696947319525 28.91411284079134 29.160066222799223 3 20.554079967603233 20.486785216593812 27.600376374182638 31.358758441620317 4 16.586071773127358 17.37097869198061 31.09553472528258 34.94741480960945 5 15.578437094296024 30.254052573279814 32.12103526721376 22.046475065210398 6 29.576936361795635 33.525887635631676 17.485915744589622 19.411260257983063 7 29.554901797305828 28.751533486582737 19.034290546576305 22.65927416953513 8 26.178253671434863 30.695339391846023 19.814433235269597 23.311973701449517 9 24.736478519277266 17.751521576007338 19.496420872092333 38.015579032623066 10-11 26.279195797949 22.90224990769304 24.99910670684501 25.81944758751295 12-13 25.432949415786275 20.701771102561963 24.871365785681107 28.99391369597065 14-15 24.412213104015056 21.948212818161245 22.38592646410748 31.25364761371622 16-17 25.497266522945722 24.06650865303303 23.37420645791398 27.062018366107267 18-19 24.377374670970354 23.46055812956324 24.335092128300715 27.826975071165688 20-21 23.97420169368382 23.943234197644088 24.716528305482438 27.366035803189654 22-23 25.70361724174895 23.49420550506795 24.031372455603332 26.77080479757977 24-25 24.720101478102407 24.443776128824783 23.728546076060937 27.107576317011873 26-27 23.733012541835897 24.547398134803892 24.645958146238044 27.073631177122166 28-29 24.493800545504353 23.94531854833907 25.831060398527857 25.729820507628727 30-31 24.465810693314594 23.42869734036851 25.655379411379364 26.450112554937526 32-33 23.47872175704808 24.493502781119357 25.627091794804606 26.400683667027952 34-35 23.957526888123965 24.228492478471637 25.73130932955371 26.08267130385069 36-37 24.273752664991246 23.387903619623867 25.735478030943675 26.60286568444122 38-39 23.278921854714802 23.451327433628318 27.180528591336245 26.08922212032063 40-41 23.181850665205637 22.662549577770104 27.581914982312796 26.573684774711463 42-43 22.097095010659963 22.187913148084185 27.69834085684679 28.016650984409058 44-45 21.166581307542966 21.936897771531346 29.663288033444896 27.233232887480796 46-47 21.01769911504425 22.48925070570159 29.811574697173622 26.68147548208054 48-49 20.26614180731071 23.214306983170356 29.785966960063842 26.733584249455088 50-51 19.951107087983424 24.430674495884897 28.488309770245003 27.12990864588668 52-53 19.22932621874963 24.684071987517715 28.249502733477055 27.8370990602556 54-55 19.201691306742894 24.12583560379353 28.34248961543615 28.329983474027426 56-57 19.32193094248383 24.328839076215772 28.163448826212793 28.185781155087604 58-59 19.562822329946762 23.905418120749413 27.775164068176135 28.756595481127693 60-61 19.793291963934777 23.338772496099285 28.115806524613202 28.752129015352736 62-63 20.10237139556212 23.811622339475218 27.156111911766455 28.9298943531962 64-65 20.750604461701545 23.763682273490634 25.79890184494813 29.686811419859694 66-67 20.76191950833145 24.311270977500925 24.617075000893294 30.30973451327434 68-69 20.816472374826926 24.492980183721173 24.663301919097176 30.02724552235473 70-71 21.23834252432735 25.2909158041425 24.451220238449718 29.01952143308043 72-73 21.920520730356483 25.876618349432462 23.464429066568208 28.738431853642847 74-75 21.37597332023166 26.968600652105945 23.847425074813525 27.80800095284887 76 21.891172424471613 26.4297242089842 23.911813574563624 27.76728979198056 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 1.0 7 1.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 1.0 15 2.0 16 4.5 17 6.0 18 8.0 19 14.5 20 24.0 21 29.0 22 39.5 23 64.5 24 99.0 25 119.0 26 187.5 27 321.5 28 470.0 29 553.0 30 710.5 31 1056.5 32 1496.0 33 1747.0 34 2032.5 35 2662.5 36 3337.5 37 3668.0 38 4138.5 39 5251.5 40 6562.5 41 7760.0 42 8289.0 43 8596.0 44 9271.5 45 9887.0 46 10134.0 47 10022.0 48 9633.5 49 9032.0 50 8707.0 51 8143.5 52 6934.0 53 5783.5 54 5279.0 55 5025.0 56 4457.5 57 4046.0 58 3948.0 59 3793.5 60 3608.5 61 3662.5 62 3747.0 63 3656.0 64 3569.0 65 3589.0 66 3487.0 67 3369.0 68 3299.0 69 3032.0 70 2639.5 71 2444.0 72 2201.0 73 1699.5 74 1171.0 75 901.0 76 710.0 77 368.0 78 152.5 79 88.0 80 55.5 81 19.0 82 16.0 83 17.0 84 15.5 85 11.0 86 6.5 87 5.0 88 5.0 89 4.5 90 4.5 91 5.0 92 5.0 93 4.5 94 4.0 95 4.5 96 5.0 97 7.0 98 7.5 99 16.5 100 27.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.977643849974392E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.977643849974392E-4 70-71 0.0 72-73 0.0 74-75 2.977643849974392E-4 76 5.955287699948784E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 167918.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.603604140116005 #Duplication Level Percentage of deduplicated Percentage of total 1 74.21905168384042 43.49503924534594 2 13.562181167814972 15.895853928703296 3 5.004776131536695 8.798937576674328 4 2.1939719122817714 5.142986457675771 5 1.2550047761315366 3.677390154718375 6 0.840395910818446 2.955013756714587 7 0.6066703249801841 2.488714729808597 8 0.4298518383025425 2.015269357662669 9 0.34042639676442493 1.7955192415345587 >10 1.544621262931122 13.627484843792804 >50 0.0030485945978903726 0.10779070736907301 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0035731726199692707 0.0 2 0.0 0.0 0.0 0.0053597589299539065 0.0 3 0.0 0.0 0.0 0.005955287699948785 0.0 4 0.0 0.0 0.0 0.009528460319918055 0.0 5 0.0 0.0 0.0 0.011315046629902691 0.0 6 0.0 0.0 0.0 0.011315046629902691 0.0 7 0.0 0.0 0.0 0.011315046629902691 0.0 8 0.0 0.0 0.0 0.01191057539989757 0.0 9 0.0 0.0 0.0 0.01548374801986684 0.0 10 0.0 0.0023821150799795137 0.0 0.01905692063983611 0.0 11 0.0 0.0023821150799795137 0.0 0.020247978179825866 0.0 12 0.0 0.0023821150799795137 0.0 0.020843506949820744 0.0 13 0.0 0.0023821150799795137 0.0 0.021439035719815626 0.0 14 0.0 0.0023821150799795137 0.0 0.021439035719815626 0.0 15 0.0 0.0023821150799795137 0.0 0.022630093259805382 0.0 16 0.0 0.0023821150799795137 0.0 0.025607737109779774 0.0 17 0.0 0.0023821150799795137 0.0 0.028585380959754166 0.0 18 0.0 0.0023821150799795137 0.0 0.029180909729749044 0.0 19 0.0 0.0023821150799795137 0.0 0.03334961111971319 0.0 20 0.0 0.0023821150799795137 0.0 0.03692278373968246 0.0 21 0.0 0.0023821150799795137 0.0 0.03811384127967222 0.0 22 0.0 0.0023821150799795137 0.0 0.04585571528960564 0.0 23 0.0 0.0023821150799795137 0.0 0.0470467728295954 0.0 24 0.0 0.0023821150799795137 0.0 0.05419311806953394 0.0 25 0.0 0.0023821150799795137 0.0 0.059552876999487844 0.0 26 0.0 0.0023821150799795137 0.0 0.07682321132933932 0.0 27 0.0 0.0023821150799795137 0.0 0.20843506949820745 0.0 28 0.0 0.0023821150799795137 0.0 0.3263497659571934 0.0 29 0.0 0.0023821150799795137 0.0 0.4728498433759335 0.0 30 0.0 0.0023821150799795137 0.0 0.6104169892447504 0.0 31 0.0 0.0023821150799795137 0.0 0.7610857680534546 0.0 32 0.0 0.0023821150799795137 0.0 0.9242606510320514 0.0 33 0.0 0.0023821150799795137 0.0 1.1404375945401923 0.0 34 0.0 0.0023821150799795137 0.0 1.3685251134482306 0.0 35 0.0 0.0023821150799795137 0.0 1.7181005014352244 0.0 36 0.0 0.0023821150799795137 0.0 2.2826617753903693 0.0 37 0.0 0.0023821150799795137 0.0 3.0991317190533474 0.0 38 0.0 0.0023821150799795137 0.0 4.019223668695434 0.0 39 0.0 0.0023821150799795137 0.0 4.935742445717553 0.0 40 0.0 0.0023821150799795137 0.0 5.992806012458462 0.0 41 0.0 0.0023821150799795137 0.0 7.362522183446682 0.0 42 0.0 0.0023821150799795137 0.0 8.857894924903823 0.0 43 0.0 0.0023821150799795137 0.0 10.393168093950619 0.0 44 0.0 0.0023821150799795137 0.0 11.724174894889172 0.0 45 0.0 0.0023821150799795137 0.0 12.883669410069201 0.0 46 0.0 0.0023821150799795137 0.0 14.05567002941912 0.0 47 0.0 0.0023821150799795137 0.0 15.192534451339345 0.0 48 0.0 0.0023821150799795137 0.0 16.14418942579116 0.0 49 0.0 0.0023821150799795137 0.0 17.1047773317929 0.0 50 0.0 0.0023821150799795137 0.0 18.022487166355006 0.0 51 0.0 0.0023821150799795137 0.0 19.045605593206208 0.0 52 0.0 0.0023821150799795137 0.0 20.236663133195965 0.0 53 0.0 0.0023821150799795137 0.0 21.683202515513525 0.0 54 0.0 0.0023821150799795137 0.0 22.935599518812754 0.0 55 0.0 0.0023821150799795137 0.0 24.005169189723556 0.0 56 0.0 0.0023821150799795137 0.0 24.926452196905633 0.0 57 0.0 0.0023821150799795137 0.0 25.78520468323825 0.0 58 0.0 0.0023821150799795137 0.0 26.590955109041317 0.0 59 0.0 0.0023821150799795137 0.0 27.356805107254733 0.0 60 0.0 0.0023821150799795137 0.0 28.085732321728464 0.0 61 0.0 0.0023821150799795137 0.0 28.87659452828166 0.0 62 0.0 0.0023821150799795137 0.0 29.66388356221489 0.0 63 0.0 0.0023821150799795137 0.0 30.578615752927025 0.0 64 0.0 0.0023821150799795137 0.0 31.459402803749448 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGTAG 15 0.002221588 70.0 14 GGTTAAG 20 0.0069417534 52.5 1 CGACAGC 35 2.0797948E-5 50.0 36 GAAAACG 35 2.0797948E-5 50.0 13 CAAGATG 35 2.0797948E-5 50.0 17 CGATTTC 45 1.677141E-6 46.666664 42 AAAACGT 40 4.577979E-5 43.75 14 TGAATTG 35 0.001252709 40.0 32 GCGACAG 45 9.168369E-5 38.888885 35 AAACGTA 45 9.168369E-5 38.888885 15 GATAGTC 45 9.168369E-5 38.888885 52 TACTAGA 55 6.66511E-6 38.18182 1 GCAAATT 40 0.0024135737 35.0 38 GATTTTC 40 0.0024135737 35.0 21 CCTACAG 40 0.0024135737 35.0 2 GTCAGTA 50 1.7042822E-4 35.0 56 TGTGAAA 50 1.7042822E-4 35.0 10 TTGCGTT 40 0.0024135737 35.0 33 AGCGATT 50 1.7042822E-4 35.0 40 AAATTCG 40 0.0024135737 35.0 40 >>END_MODULE