FastQCFastQC Report
Wed 25 May 2016
SRR1780292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167918
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT83134.9506306649674245No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC23011.3703116997582154No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG22661.3494681928083947No Hit
CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG18291.0892221203206327RNA PCR Primer, Index 18 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC5430.323372122107219No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT3170.18878262008837646No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2620.15602853773865816RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT2010.11970128276897057No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCAGA150.00222158870.025
CTTTATA750.060.6666682
TTAATAC452.6850103E-854.4444433
GTACAAG407.4457785E-752.51
CGACATA200.006941753452.531
GATAGTC200.006941753452.552
TACCGGC200.006941753452.556
TCTTTAT1000.045.4999961
CTTAATA602.5821464E-740.8333322
GCTCTAA400.002413573735.01
GTCACTC400.002413573735.08
CAAGGAA601.2103814E-535.04
TATGCCG17650.032.52124843
CGTATGC17700.032.4293841
TCGTATG17700.032.4293840
CTTGAAA17400.032.38505657
GTATGCC17750.032.33802842
CTCGTAT17550.032.3076939
CCGTCTT17600.032.2159147
CGTCTTC17650.032.12464548