FastQCFastQC Report
Wed 25 May 2016
SRR1780291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630546
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTG250.002335389135.250717
GTACAAG506.004484E-835.1556551
TTAGCGC353.230961E-431.4039231
CTTAGCG407.050861E-427.48497230
GACCTAT450.001407773124.4136471
ATTTTGA551.5983527E-423.98688330
AGCCTTA551.6052488E-423.9716646
GTATAAG1900.023.128721
AGAGACA2350.022.4415596
TTTAACG500.00256470522.02118134
GTAACAC500.002571815622.01068524
TTACATT602.8812952E-421.98797826
ATATAGA703.241629E-521.9740266
CTCTAAT500.00259802721.9722841
AAGAGAC2350.021.5064955
CAGAGTT755.4269367E-520.553134
GGTAAGT755.5004362E-520.51722731
CTGCCGA4900.020.23318318
TAAGAGA2500.020.2161064
CGAAACT550.004495469419.98906925
Produced by FastQC (version 0.11.5)