FastQCFastQC Report
Wed 25 May 2016
SRR1780291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630546
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT183722.9136652995974917No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC50180.7958182273775427No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG48900.7755183602782351No Hit
CACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCTG31300.49639518766275575TruSeq Adapter, Index 5 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC11410.1809542840649215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT25800.033.76549544
CGTCTTC4150.033.39566442
TCTTCTG4200.032.99809644
CCGTCTT4450.031.14426841
GTCTTCT4450.031.14426843
TCTTTAT1750.030.2320211
GCTTATA454.063692E-529.3362963
CTTTATA1800.029.3362962
TATCAGT300.005744575529.3316430
CTAACTG300.005744575529.3316442
AAACAGG300.005744575529.3316438
TATGCCG31650.027.52447943
CAATAGT407.0307456E-427.49841515
GCCGTCT5800.024.65375140
CATTGAA450.001397942124.44303726
TAAGGTG551.5930625E-423.99861520
GTATGCC38600.022.56864542
CTCGTAT38700.022.28295339
CGTATGC39200.022.27932741
TCGTATG39150.022.2515940
Produced by FastQC (version 0.11.5)