FastQCFastQC Report
Wed 25 May 2016
SRR1780290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136383
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAC150.002220689870.056
ATCGAAT207.90362E-570.070
ACGAATA150.002220689870.066
CACGGTT200.006938968352.549
TTCCGGG200.006938968352.516
CGTTACT200.006938968352.52
AATGCAC200.006938968352.564
AAAGCGC200.006938968352.58
CATTGAA305.8610906E-446.66666426
CGGACTA305.8610906E-446.6666649
TAAGGAA350.001251881140.021
AGGGGAG556.655895E-638.18181639
ACTAGCC400.002411987635.039
CGCTCGG400.002411987635.010
GCTTCAA400.002411987635.062
AACTAGC400.002411987635.038
TTCATAC552.979735E-431.818183
GTCCTAC450.004295284431.1111091
ATCCCGG450.004295284431.11110911
ATGACAA450.004295284431.11110930
Produced by FastQC (version 0.11.5)