##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780290_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 136383 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.463496183541938 34.0 32.0 34.0 12.0 34.0 2 30.516574646400212 34.0 32.0 34.0 12.0 34.0 3 30.280746134048965 34.0 32.0 34.0 11.0 34.0 4 30.206360030209044 34.0 31.0 34.0 11.0 34.0 5 30.1891291436616 34.0 31.0 34.0 11.0 34.0 6 33.25690885227631 38.0 34.0 38.0 21.0 38.0 7 33.39057653813158 38.0 34.0 38.0 21.0 38.0 8 33.3615553258104 38.0 34.0 38.0 11.0 38.0 9 33.64587228613537 38.0 34.0 38.0 21.0 38.0 10-11 33.4227286391999 38.0 34.0 38.0 16.0 38.0 12-13 33.49784063996246 38.0 34.0 38.0 21.0 38.0 14-15 33.43098480015838 38.0 34.0 38.0 11.0 38.0 16-17 33.47619937968808 38.0 34.0 38.0 16.0 38.0 18-19 33.485980657413315 38.0 34.0 38.0 16.0 38.0 20-21 33.50813517813803 38.0 34.0 38.0 16.0 38.0 22-23 33.51207628516751 38.0 34.0 38.0 16.0 38.0 24-25 33.52194555039851 38.0 34.0 38.0 16.0 38.0 26-27 33.51871200956131 38.0 34.0 38.0 16.0 38.0 28-29 33.56553236107139 38.0 34.0 38.0 11.0 38.0 30-31 33.593233760805965 38.0 34.0 38.0 16.0 38.0 32-33 33.67103304664071 38.0 34.0 38.0 21.0 38.0 34-35 33.620326580292264 38.0 34.0 38.0 16.0 38.0 36-37 33.5363864997837 38.0 34.0 38.0 16.0 38.0 38-39 33.54060623391479 38.0 34.0 38.0 16.0 38.0 40-41 33.59824538248902 38.0 34.0 38.0 11.0 38.0 42-43 33.667627196938035 38.0 34.0 38.0 21.0 38.0 44-45 33.74008857408914 38.0 34.0 38.0 21.0 38.0 46-47 33.68796697535616 38.0 34.5 38.0 16.0 38.0 48-49 33.61832486453591 38.0 34.0 38.0 16.0 38.0 50-51 33.57706972276603 38.0 34.0 38.0 15.0 38.0 52-53 33.625884457740334 38.0 34.0 38.0 21.0 38.0 54-55 33.60811464772003 38.0 34.0 38.0 20.5 38.0 56-57 33.565239069385484 38.0 34.0 38.0 15.0 38.0 58-59 33.632157233672814 38.0 34.0 38.0 20.5 38.0 60-61 33.63940520446097 38.0 34.0 38.0 20.0 38.0 62-63 33.52947214828828 38.0 34.0 38.0 19.0 38.0 64-65 33.49120124942258 38.0 34.0 38.0 19.0 38.0 66-67 33.402201887332 38.0 34.0 38.0 19.0 38.0 68-69 33.343664532969655 38.0 34.0 38.0 14.5 38.0 70-71 33.37350329586532 38.0 34.0 38.0 15.0 38.0 72-73 33.38789291920547 38.0 34.0 38.0 14.5 38.0 74-75 33.41541834392849 38.0 34.0 38.0 15.0 38.0 76 31.956937448215687 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5742243765498038 1101 2 0.39219785724566236 1101 3 0.6433654265544 1101 4 0.8069020938605114 1101 5 -0.3127660278463331 1101 6 0.5208692545576312 1101 7 0.3417906067415899 1101 8 0.05050512776885796 1101 9 0.42768331762287914 1101 10-11 0.09202316115386111 1101 12-13 0.4457867330873171 1101 14-15 0.2616834178462568 1101 16-17 0.4171577768785042 1101 18-19 0.4204551086998052 1101 20-21 0.04391147286733599 1101 22-23 0.27140484936258247 1101 24-25 0.029050302970425435 1101 26-27 -0.03570924870967929 1101 28-29 -0.13700967738758152 1101 30-31 -0.03315379374682692 1101 32-33 -0.5051660196712717 1101 34-35 0.012355125991668103 1101 36-37 -0.15708318990673575 1101 38-39 0.12374732892219242 1101 40-41 0.13206555258275188 1101 42-43 0.0037337268384618483 1101 44-45 0.03240622645539304 1101 46-47 0.27851634128510483 1101 48-49 -0.19741317888721 1101 50-51 -0.10530840071826475 1101 52-53 -0.058817163829907315 1101 54-55 -0.3310352716274707 1101 56-57 0.05878621697202391 1101 58-59 0.17271202634172766 1101 60-61 0.08188998985642826 1101 62-63 -0.20115216441144668 1101 64-65 -0.35537549562021553 1101 66-67 -0.0764567885154861 1101 68-69 -0.03538338265613561 1101 70-71 0.4164594961060999 1101 72-73 0.06999520446947827 1101 74-75 -0.15198816677708038 1101 76 -1.2730585049166443 1102 1 0.10420507934167489 1102 2 0.0653591756807188 1102 3 0.30392909177896144 1102 4 0.6888000676536556 1102 5 0.265317634888266 1102 6 -0.02356101093460694 1102 7 -0.6496423665846933 1102 8 -0.24564476796396661 1102 9 0.10549775317954158 1102 10-11 0.14625376279101232 1102 12-13 0.11660982126311126 1102 14-15 0.12298862497999608 1102 16-17 0.33406695550218046 1102 18-19 0.27499851185582713 1102 20-21 0.15650834796460344 1102 22-23 0.23116861418563417 1102 24-25 -0.06189557147322233 1102 26-27 -0.1225622475580721 1102 28-29 0.02086171605759546 1102 30-31 0.07895676058104328 1102 32-33 0.28836941560526697 1102 34-35 0.007266265460692978 1102 36-37 0.1716112917142283 1102 38-39 0.32190942547048707 1102 40-41 0.03862971579977881 1102 42-43 0.17009822620040893 1102 44-45 0.19988731830387962 1102 46-47 -0.1605068153646485 1102 48-49 0.04939266629938288 1102 50-51 -0.06083533899832361 1102 52-53 -0.0624665148834751 1102 54-55 0.008891274483517009 1102 56-57 -0.08995790827307815 1102 58-59 -0.0955697440214962 1102 60-61 -0.046895531012566494 1102 62-63 0.17500333373664034 1102 64-65 0.1510672819327752 1102 66-67 0.05052740022365754 1102 68-69 -0.030224491934362163 1102 70-71 0.09788826762093095 1102 72-73 0.0709250497805698 1102 74-75 -0.1957570360229326 1102 76 -0.10589643949288075 1103 1 0.4469392262309455 1103 2 0.3430936969055445 1103 3 0.6928188125246386 1103 4 0.4826385044283832 1103 5 0.4546041513215968 1103 6 0.06435543736965599 1103 7 0.7116812839356328 1103 8 0.3387529261290183 1103 9 0.13885853778686652 1103 10-11 0.19125638313989413 1103 12-13 0.5535380237037799 1103 14-15 -0.11559414061107987 1103 16-17 0.2977302032374496 1103 18-19 0.5441644922406468 1103 20-21 0.35313558461954386 1103 22-23 0.0541865074251362 1103 24-25 0.4600554654362625 1103 26-27 0.16377450470665877 1103 28-29 0.40049411854318606 1103 30-31 0.15582282331967434 1103 32-33 0.29079510999772396 1103 34-35 0.09899344970865087 1103 36-37 0.3449286533972469 1103 38-39 -0.01894930029128261 1103 40-41 0.03599813685240605 1103 42-43 -0.06312779760733633 1103 44-45 0.4002167335917406 1103 46-47 0.3350984852268013 1103 48-49 0.08413100574686183 1103 50-51 -0.2659749661601083 1103 52-53 0.12634833875132045 1103 54-55 0.14003973596437902 1103 56-57 0.20189280670783205 1103 58-59 -0.11479936145055802 1103 60-61 0.42276457230404674 1103 62-63 0.1545720991779831 1103 64-65 0.21384709775968247 1103 66-67 0.2755854222345917 1103 68-69 0.3506020409697328 1103 70-71 0.056157340017428226 1103 72-73 0.1398327386328475 1103 74-75 -0.13642485065196297 1103 76 0.452799375435621 1104 1 0.4634886523694135 1104 2 0.1944450482568989 1104 3 0.2757880831651427 1104 4 0.5347303222736244 1104 5 0.04405250378365011 1104 6 0.3362917572511819 1104 7 -0.4067933550869469 1104 8 0.12053745037929531 1104 9 -0.0478130653230906 1104 10-11 0.38007533908213276 1104 12-13 0.46003561718767116 1104 14-15 0.0807960852311993 1104 16-17 0.363205952959774 1104 18-19 0.4527353919074386 1104 20-21 0.15696749872543592 1104 22-23 0.36086921753250323 1104 24-25 -0.0769678592582963 1104 26-27 0.3729562892309062 1104 28-29 0.6804830115457747 1104 30-31 0.3982145300949469 1104 32-33 0.42233898263748415 1104 34-35 0.8942544641477426 1104 36-37 0.43767208175711403 1104 38-39 0.14200960381830185 1104 40-41 0.06545019164693144 1104 42-43 0.17552455337378348 1104 44-45 0.6190616058612761 1104 46-47 0.4098115544604184 1104 48-49 0.3836126792011427 1104 50-51 0.4535918609409677 1104 52-53 0.19139832023966363 1104 54-55 0.532209302791486 1104 56-57 0.25507681263155746 1104 58-59 0.4644120417334534 1104 60-61 0.4616575748956748 1104 62-63 0.4052200073601355 1104 64-65 0.5015738656234348 1104 66-67 1.024678350779574 1104 68-69 0.512430993209648 1104 70-71 0.5320551556412738 1104 72-73 0.2809656523685078 1104 74-75 0.24269399422030347 1104 76 0.6974124816925631 1105 1 0.14607749493443123 1105 2 -0.017149154641654007 1105 3 0.4230185775070119 1105 4 0.8915300046242294 1105 5 1.0451642766643339 1105 6 0.9550330715255484 1105 7 0.8009096427292093 1105 8 0.3873232355688927 1105 9 1.0006681735095455 1105 10-11 0.7871827442836477 1105 12-13 0.03138761242293242 1105 14-15 0.6305280685546109 1105 16-17 0.26872511118888553 1105 18-19 0.7329815701881586 1105 20-21 0.6270290432956074 1105 22-23 0.7069283797322328 1105 24-25 0.41348280582011654 1105 26-27 0.5181419155958125 1105 28-29 0.09753681341196341 1105 30-31 0.63369199684103 1105 32-33 0.5909909580196597 1105 34-35 0.7652292507270531 1105 36-37 0.7601656295037884 1105 38-39 0.8933695402884183 1105 40-41 0.8787517600408208 1105 42-43 0.5268447836695955 1105 44-45 0.9390338115392538 1105 46-47 0.7535081992886887 1105 48-49 0.4466065247441193 1105 50-51 0.5462263656064366 1105 52-53 0.4704533132910811 1105 54-55 0.4580381691802131 1105 56-57 0.26873573659423755 1105 58-59 0.2607392206097643 1105 60-61 0.6111116149989115 1105 62-63 0.615245816373438 1105 64-65 0.7112423190679493 1105 66-67 0.6251349717841421 1105 68-69 0.5675233100649137 1105 70-71 0.32286317628848593 1105 72-73 0.5182894562200033 1105 74-75 0.6168849809187407 1105 76 0.8540136331716148 1106 1 -0.38279050349878574 1106 2 -0.15690630309445552 1106 3 0.7198458395516667 1106 4 0.5285844503743284 1106 5 -0.3562066594892137 1106 6 0.18597705899127703 1106 7 -0.13156195930761072 1106 8 0.009023196399283506 1106 9 0.41723691616524405 1106 10-11 -0.14387875199523847 1106 12-13 0.0062407264064745505 1106 14-15 0.32089234587540716 1106 16-17 0.44502750053474927 1106 18-19 -0.09097064289449008 1106 20-21 0.038270719754677884 1106 22-23 -0.2093270060883725 1106 24-25 0.2641095194903116 1106 26-27 0.03674747767963282 1106 28-29 -0.1468031787541193 1106 30-31 -0.10633933884248137 1106 32-33 0.3097140289166447 1106 34-35 3.4480106000245314E-4 1106 36-37 0.10033405880265178 1106 38-39 0.1634831321411525 1106 40-41 0.3184305692848426 1106 42-43 0.15038571590618233 1106 44-45 -0.28221569884069453 1106 46-47 -0.060847460719145374 1106 48-49 0.6192661408819973 1106 50-51 0.09618719600205594 1106 52-53 0.412834825237816 1106 54-55 0.15625595218060084 1106 56-57 0.30054145535648047 1106 58-59 0.35141685476565954 1106 60-61 0.15789970743917792 1106 62-63 0.05862615323203357 1106 64-65 0.046279530192109064 1106 66-67 -0.08522538857622663 1106 68-69 0.345196635564335 1106 70-71 -0.14789671403661941 1106 72-73 -0.006113207313092062 1106 74-75 0.3487102844831682 1106 76 0.8392858619221037 1107 1 0.082012936304654 1107 2 -0.29475949719764927 1107 3 0.15651660122242816 1107 4 0.11573125627113257 1107 5 0.47828351250095835 1107 6 -0.45120045943624376 1107 7 -0.11751097650662246 1107 8 -0.15822188959580075 1107 9 -0.378432617004286 1107 10-11 -0.29593649155298607 1107 12-13 0.1459750630158112 1107 14-15 -0.07935335437029778 1107 16-17 0.11373910987136071 1107 18-19 0.28184108929224294 1107 20-21 0.20692858216253995 1107 22-23 0.050723669587299014 1107 24-25 0.4988377135935096 1107 26-27 0.4685042344363879 1107 28-29 0.08914583844489954 1107 30-31 0.13168277417963026 1107 32-33 0.33269468002229274 1107 34-35 0.18641666838186666 1107 36-37 -0.2733529805093866 1107 38-39 -0.1365629366549257 1107 40-41 0.04849813685240889 1107 42-43 -0.047817601047142944 1107 44-45 -0.1811407602657482 1107 46-47 0.2233739766272933 1107 48-49 0.08816202540288742 1107 50-51 0.32951029182515157 1107 52-53 -0.13593667353368488 1107 54-55 0.1295590848587409 1107 56-57 0.04360810154812356 1107 58-59 -0.3026295211557155 1107 60-61 -0.11017461688514629 1107 62-63 0.21352480188068768 1107 64-65 0.38337258916017447 1107 66-67 0.1633925475417115 1107 68-69 0.33083852745622266 1107 70-71 0.3590827085677972 1107 72-73 0.4473573086574163 1107 74-75 -0.10329370814582006 1107 76 0.3636562550424962 1108 1 -0.993765566960036 1108 2 -1.134836719698903 1108 3 -0.49598214312714717 1108 4 -0.40183215407542505 1108 5 -0.42926922481697005 1108 6 0.16107038436084764 1108 7 -0.3722667524985894 1108 8 -0.4696388911025835 1108 9 -0.5236171976170425 1108 10-11 -0.27374287025715205 1108 12-13 -0.5354271845984471 1108 14-15 -0.24710825140695647 1108 16-17 -0.4855906867132731 1108 18-19 -0.42018521658420127 1108 20-21 -0.590958785994161 1108 22-23 0.004193973454704292 1108 24-25 -0.5947003953969485 1108 26-27 -0.8719653788232833 1108 28-29 -0.10913705959628572 1108 30-31 -0.13960050055668205 1108 32-33 0.11323052884198148 1108 34-35 -0.12826502423491348 1108 36-37 -0.4101482467072799 1108 38-39 -0.3170155986338301 1108 40-41 -0.4021234101089206 1108 42-43 -0.24635162917370934 1108 44-45 -0.4033594945701253 1108 46-47 -0.25707711578858294 1108 48-49 -0.4979206591777299 1108 50-51 -0.28993345503572954 1108 52-53 -0.6866649507812994 1108 54-55 -0.29773090760735954 1108 56-57 -0.5178324434041599 1108 58-59 -0.37292240161779944 1108 60-61 -0.06752958926083608 1108 62-63 -0.42200696607510935 1108 64-65 -0.28302463585238513 1108 66-67 -0.5360989140353851 1108 68-69 -0.34716561619018904 1108 70-71 -0.3382506038380271 1108 72-73 -0.3830357099262045 1108 74-75 -0.3397248357199061 1108 76 -0.53876401115539 1109 1 0.3488582183938398 1109 2 0.580894912755543 1109 3 0.2563872590199132 1109 4 0.5113721160888218 1109 5 0.25034867633441493 1109 6 0.3956599125765692 1109 7 0.43241665345149016 1109 8 0.19964796751186498 1109 9 0.7940698466567113 1109 10-11 0.9543560681907977 1109 12-13 0.7526554337967966 1109 14-15 0.6411443051517551 1109 16-17 0.7846671401743848 1109 18-19 0.6659142908197069 1109 20-21 0.6519966407976696 1109 22-23 0.9593025991265947 1109 24-25 0.4299668402256813 1109 26-27 0.7777860772548166 1109 28-29 0.6930122464271662 1109 30-31 0.5736943533863297 1109 32-33 0.2878214045849887 1109 34-35 0.6563215094757382 1109 36-37 0.4391570281134278 1109 38-39 0.8454138433401539 1109 40-41 0.5740740176112169 1109 42-43 0.4464158924241559 1109 44-45 0.96410745358002 1109 46-47 0.6981176019044497 1109 48-49 0.4138387622838877 1109 50-51 0.4948065470604277 1109 52-53 0.5766156792625665 1109 54-55 0.6837480784532204 1109 56-57 0.6197313769854276 1109 58-59 0.7209253876888226 1109 60-61 0.4293748411094356 1109 62-63 0.46784390757174066 1109 64-65 0.30247597053489983 1109 66-67 0.605080771242136 1109 68-69 0.4904760613315631 1109 70-71 0.8226628699375951 1109 72-73 0.1788644532743291 1109 74-75 0.5388479831574529 1109 76 0.17436240187669583 1110 1 0.19871969751002538 1110 2 0.020628892268039323 1110 3 0.3739450641244275 1110 4 -0.4459694907534306 1110 5 0.039359101257606 1110 6 -0.08950287776999488 1110 7 0.2561516596037805 1110 8 0.1085940764715616 1110 9 0.13747330854648254 1110 10-11 0.23142803111097976 1110 12-13 0.3104114709671961 1110 14-15 0.3429054453258331 1110 16-17 0.38540994425980557 1110 18-19 0.04885093355661496 1110 20-21 -0.32074456293748455 1110 22-23 -0.27626971481381446 1110 24-25 0.01679716535344511 1110 26-27 0.004367744185692857 1110 28-29 0.06158348088602139 1110 30-31 -0.3744263673751931 1110 32-33 -0.3514619102537324 1110 34-35 0.05636000846796918 1110 36-37 -0.0590463522490694 1110 38-39 -0.14722228079267552 1110 40-41 0.041708443259089734 1110 42-43 0.03502773954541283 1110 44-45 -0.04732523734855931 1110 46-47 0.0022143476072855606 1110 48-49 0.23116104414180683 1110 50-51 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9 -0.42919640239922074 2116 10-11 0.09654363760747486 2116 12-13 0.07101697806797347 2116 14-15 0.220323744487132 2116 16-17 0.09067806913613197 2116 18-19 -0.09599205775196396 2116 20-21 -0.19162146751455822 2116 22-23 -0.12080980035806022 2116 24-25 -0.3429795367790689 2116 26-27 -0.03764330654721704 2116 28-29 0.03370930093868907 2116 30-31 0.11969817408027694 2116 32-33 -0.02037753806017406 2116 34-35 0.3807317453813752 2116 36-37 0.21400264542205605 2116 38-39 0.1307774756649991 2116 40-41 -0.3446029560437651 2116 42-43 0.10377517093498057 2116 44-45 0.20432247868109243 2116 46-47 0.13650891204706284 2116 48-49 0.1514770403060126 2116 50-51 0.24198545327571708 2116 52-53 0.03097837862726749 2116 54-55 0.01166135758600717 2116 56-57 -0.17726093720001757 2116 58-59 -0.03138479934747096 2116 60-61 -0.1284464748086407 2116 62-63 0.17066278002275936 2116 64-65 0.20241599452529613 2116 66-67 -0.010384153898918669 2116 68-69 0.3709683088769893 2116 70-71 0.20742007816541275 2116 72-73 0.28084588292468027 2116 74-75 0.1100306212133404 2116 76 0.7015070995531794 2117 1 0.2847770640687841 2117 2 0.005255859067709423 2117 3 -0.001775782069955767 2117 4 -0.052496630706752256 2117 5 0.2005500972675449 2117 6 0.38327435628857387 2117 7 0.41708668934104054 2117 8 0.13875292612901546 2117 9 -0.1341144351861061 2117 10-11 0.14260773449124287 2117 12-13 0.004889375055128653 2117 14-15 0.4952166701997385 2117 16-17 0.1382707437779942 2117 18-19 0.05092124899740469 2117 20-21 -0.21443198294802812 2117 22-23 0.24067299391162322 2117 24-25 0.11951492489571791 2117 26-27 0.24080153173368757 2117 28-29 -0.10220858415951994 2117 30-31 0.10987687737372909 2117 32-33 -0.015961646759038217 2117 34-35 0.06115561187080942 2117 36-37 -0.09696323849465216 2117 38-39 0.14456421322222468 2117 40-41 0.21437651523078927 2117 42-43 0.1247100402305037 2117 44-45 0.247514030889036 2117 46-47 0.19455794468625953 2117 48-49 0.26656343817929695 2117 50-51 0.588079087893945 2117 52-53 0.20878077118375415 2117 54-55 0.1130127089373616 2117 56-57 0.13973064454566497 2117 58-59 -0.014799361450556603 2117 60-61 0.0592510587905295 2117 62-63 -0.03191438730850393 2117 64-65 -0.21588263197004665 2117 66-67 0.255315151964318 2117 68-69 -0.222370932003237 2117 70-71 0.027778961639050692 2117 72-73 -0.5466537478536324 2117 74-75 -0.32291133713845 2117 76 -0.32828170564546255 2118 1 -0.40476561212239304 2118 2 -0.29928792177035035 2118 3 -0.2012676445045969 2118 4 -0.5751050702089273 2118 5 -1.0116378399885129 2118 6 -0.4295027259707993 2118 7 -0.18517415460917874 2118 8 -0.004541867302016556 2118 9 -0.44610353600354813 2118 10-11 -0.98108325895452 2118 12-13 -0.6455819409475225 2118 14-15 -0.4079389377242677 2118 16-17 -0.3438523875277042 2118 18-19 -0.719385842835571 2118 20-21 -0.39117180178593713 2118 22-23 -0.29196195711570283 2118 24-25 -0.533972077092649 2118 26-27 -0.2607191988061217 2118 28-29 -0.46376319053701565 2118 30-31 -0.21997068135666353 2118 32-33 -0.7767577289447587 2118 34-35 -1.2389843100674724 2118 36-37 -0.8504097367640355 2118 38-39 -0.6746713711359718 2118 40-41 -0.986753906744319 2118 42-43 -0.7209892012453025 2118 44-45 -0.6413989965798521 2118 46-47 -1.1666394925329726 2118 48-49 -1.113453499739549 2118 50-51 -0.46574299031505717 2118 52-53 -0.7539079393251171 2118 54-55 -0.5373849639398784 2118 56-57 -0.6413025685038889 2118 58-59 -0.6307467802590097 2118 60-61 -0.5205118103456385 2118 62-63 -0.8637061245498288 2118 64-65 -0.5787628146049997 2118 66-67 -0.3599694787154064 2118 68-69 -0.38007768507783624 2118 70-71 -0.34977829589095677 2118 72-73 -0.3786737787837495 2118 74-75 -0.7221822701968534 2118 76 -0.4633579262802634 2119 1 0.5688031069183559 2119 2 0.36195205105801875 2119 3 -0.7037138545962627 2119 4 0.6810562499049517 2119 5 0.3794962703313658 2119 6 0.3620236938614383 2119 7 0.8611702687187375 2119 8 0.5004154289679903 2119 9 0.4835326236374229 2119 10-11 0.5570524312888807 2119 12-13 0.7542837273906571 2119 14-15 0.4644459835466961 2119 16-17 0.3855074763088453 2119 18-19 0.7109257913548888 2119 20-21 0.6714450705760839 2119 22-23 0.29463998611390707 2119 24-25 0.5697609692594128 2119 26-27 0.44504485068288346 2119 28-29 0.5075234167489526 2119 30-31 0.18111618390715734 2119 32-33 0.4175041720009034 2119 34-35 0.3763119446741996 2119 36-37 0.6572914363482596 2119 38-39 0.2341319322017057 2119 40-41 0.08123623209050379 2119 42-43 0.42085282166740257 2119 44-45 0.29232817276200507 2119 46-47 0.3908483527413722 2119 48-49 0.20348144862671802 2119 50-51 -0.012560627451655648 2119 52-53 -0.005493587208249551 2119 54-55 0.24555491500897375 2119 56-57 -0.07437716073860656 2119 58-59 0.07279621732149621 2119 60-61 0.16704877247059358 2119 62-63 0.19312914361737654 2119 64-65 0.015395284601986248 2119 66-67 -0.054696960988970034 2119 68-69 -0.05422799915442056 2119 70-71 -0.07710028735784391 2119 72-73 0.21472107234471594 2119 74-75 0.31815157451269727 2119 76 0.21565046181612146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 13.0 13 204.0 14 967.0 15 2045.0 16 2682.0 17 3029.0 18 3168.0 19 2695.0 20 2091.0 21 1561.0 22 1152.0 23 980.0 24 888.0 25 881.0 26 977.0 27 1178.0 28 1384.0 29 1657.0 30 2031.0 31 2642.0 32 3303.0 33 4217.0 34 5932.0 35 9011.0 36 17217.0 37 64478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.55880864917182 23.850479898521076 12.110013711386317 27.48069774092079 2 18.970839474128006 22.788030766297855 31.79648489914432 26.44464486042982 3 17.92305492620048 22.783631391009145 29.665720800979596 29.627592881810784 4 14.990138067061142 18.944443222395755 31.0287939112646 35.036624799278506 5 15.479935182537414 32.30754566184935 33.09650029695783 19.116018858655405 6 28.386235821180062 35.225797936692985 17.12970091580329 19.258265326323663 7 26.914644787106894 26.327328186064246 20.718124692960266 26.039902333868593 8 23.67834464958719 30.552418940915953 21.574694607792814 24.194541801704037 9 22.99993400937067 17.598967613265586 21.415425676220643 37.9856727011431 10-11 25.94861529662788 23.325121166127744 24.972687211749264 25.753576325495114 12-13 24.305082011687674 21.228085611843117 24.732921258514622 29.73391111795458 14-15 23.797320780449176 22.45074532749683 22.766033889854306 30.98590000219969 16-17 24.640534377451733 23.99199313697455 23.738295828659 27.629176656914716 18-19 23.704200670171502 23.23676704574617 24.656665420177003 28.402366863905325 20-21 23.64480910377393 23.832515782758847 24.599473541423787 27.92320157204344 22-23 25.359832237155654 23.375347367340503 23.667172594824866 27.59764780067897 24-25 24.436696655741553 24.144871428257186 23.185807615318623 28.232624300682634 26-27 23.383046274095744 24.36080743201132 24.66106479546571 27.59508149842722 28-29 23.982094542574956 23.89190734915642 25.77154044125734 26.354457667011282 30-31 24.20646268229911 23.352617261682173 25.356166091081732 27.08475396493698 32-33 23.18654084453341 24.486922856954312 25.411891511405383 26.914644787106894 34-35 23.608600810221255 23.9513867248364 25.747071655087712 26.692940809854637 36-37 24.04038626515035 23.067757711738267 25.52554203969703 27.366313983414354 38-39 22.75393560781036 23.27196204805584 27.52652456684484 26.44757777728896 40-41 22.81369378881532 22.52553470740488 27.73402843462895 26.92674306915085 42-43 21.64199350358916 22.60252377495729 27.707998797504086 28.047483923949468 44-45 20.901798611263867 22.575760908617642 28.927725596298657 27.594714883819833 46-47 21.181892171311674 23.281494027848044 28.764582096009033 26.772031704831246 48-49 20.73828849636685 23.541790399096662 28.63956651488822 27.08035458964827 50-51 20.869169911205944 24.354941598293042 27.340284346289494 27.435604144211524 52-53 19.81881906102667 24.51551879633092 27.653006606395223 28.012655536247188 54-55 20.002126364722876 24.111876113591872 27.547788214073606 28.33820930761165 56-57 20.324013990013416 24.226259871098303 27.406274975620125 28.043451163268152 58-59 20.78228224925394 23.92123651774781 26.678911594553572 28.617569638444674 60-61 20.58064421518811 23.42923971462719 26.964504373712266 29.025611696472435 62-63 20.525285409471856 23.467367633796 26.72253873283327 29.28480822389887 64-65 21.085839144174862 23.875043077216368 25.629293973589085 29.409823805019684 66-67 21.050644141865188 24.5455811941371 24.598007082994215 29.805767581003494 68-69 20.849006107799358 25.154528057015902 24.656665420177003 29.339800415007733 70-71 21.189591078066915 25.860261176246308 24.249356591364027 28.70079115432275 72-73 21.30837421086206 25.66668866354311 24.34797592075259 28.676961204842243 74-75 21.13313242852848 26.578092577520657 24.872601423931133 27.416173570019726 76 21.314240044580337 26.233474846571784 24.825674754184906 27.626610354662972 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 3.0 14 5.5 15 7.5 16 11.0 17 12.0 18 15.0 19 20.0 20 31.0 21 40.0 22 57.5 23 89.0 24 139.0 25 175.0 26 263.5 27 419.0 28 569.0 29 652.0 30 800.0 31 1115.5 32 1503.5 33 1724.0 34 1983.0 35 2579.0 36 3170.5 37 3425.0 38 3805.5 39 4800.5 40 5869.5 41 6526.5 42 6729.0 43 6919.0 44 7396.5 45 7644.0 46 7604.0 47 7722.5 48 7457.5 49 6702.0 50 6330.0 51 5824.0 52 4849.0 53 4040.0 54 3700.0 55 3576.0 56 3256.0 57 2974.0 58 2888.0 59 2803.5 60 2675.5 61 2650.0 62 2668.0 63 2706.5 64 2771.0 65 2763.0 66 2773.5 67 2818.0 68 2810.5 69 2707.0 70 2408.0 71 2205.0 72 2100.5 73 1779.0 74 1335.5 75 1109.0 76 918.5 77 543.5 78 253.0 79 147.0 80 95.5 81 32.5 82 17.0 83 13.0 84 13.0 85 10.0 86 5.0 87 3.0 88 2.0 89 1.5 90 3.0 91 3.5 92 3.0 93 4.5 94 6.0 95 5.5 96 5.0 97 5.0 98 4.5 99 16.0 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 7.332292147848339E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.6661460739241693E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 136383.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.03741668683047 #Duplication Level Percentage of deduplicated Percentage of total 1 74.2128991393122 43.813378500252966 2 13.152501956108647 15.529794769142782 3 5.0026702435510515 8.860341831459932 4 2.4280586708396985 5.733852459617401 5 1.3549933554404658 3.9997653666512685 6 0.9091247811021275 3.2203427113349905 7 0.6334066097842692 2.617628296781857 8 0.5203869990188408 2.4577843279587634 9 0.38873778208328674 2.065506698048877 >10 1.3972204627594174 11.701605038751165 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007332292147848339 0.0 2 0.0 0.0 0.0 0.012464896651342176 0.0 3 0.0 0.0 0.0 0.01319812586612701 0.0 4 0.0 0.0 0.0 0.06819031697498955 0.0 5 0.0 0.0 0.0 0.07845552598197722 0.0 6 0.0 0.0 0.0 0.07845552598197722 0.0 7 0.0 0.0 0.0 0.08065521362633173 0.0 8 0.0 0.0 0.0 0.08065521362633173 0.0 9 0.0 0.0 0.0 0.08798750577418006 0.0 10 0.0 0.0 0.0 0.09531979792202841 0.0 11 0.0 0.0 0.0 0.09605302713681324 0.0 12 0.0 0.0 0.0 0.0975194855663829 0.0 13 0.0 0.0 0.0 0.09825271478116775 0.0 14 0.0 0.0 0.0 0.10191886085509191 0.0 15 0.0 0.0 0.0 0.10191886085509191 0.0 16 0.0 0.0 0.0 0.10265209006987674 0.0 17 0.0 0.0 0.0 0.10338531928466158 0.0 18 0.0 0.0 0.0 0.10485177771423125 0.0 19 0.0 0.0 0.0 0.11585021593600375 0.0 20 0.0 0.0 0.0 0.11878313279514309 0.0 21 0.0 0.0 0.0 0.12024959122471275 0.0 22 0.0 0.0 0.0 0.12611542494299144 0.0 23 0.0 0.0 0.0 0.13124802944648525 0.0 24 0.0 0.0 0.0 0.13344771709083977 0.0 25 0.0 0.0 0.0 0.1363806339499791 0.0 26 0.0 0.0 0.0 0.1510452182456758 0.0 27 0.0 0.0 0.0 0.23023397344243784 0.0 28 0.0 0.0 0.0 0.33215283429752973 0.0 29 0.0 0.0 0.0 0.43627138279697614 0.0 30 0.0 0.0 0.0 0.5623868077399676 0.0 31 0.0 0.0 0.0 0.7082994214821495 0.0 32 0.0 0.0 0.0 0.8468797430764832 0.0 33 0.0 0.0 0.0 1.0360528804909703 0.0 34 0.0 0.0 0.0 1.3300777956196888 0.0 35 0.0 0.0 0.0 1.6717626097094214 0.0 36 0.0 0.0 0.0 2.201887331998856 0.0 37 0.0 0.0 0.0 2.8933224815409546 0.0 38 0.0 0.0 0.0 3.6346172176884215 0.0 39 0.0 0.0 0.0 4.396442371849864 0.0 40 0.0 0.0 0.0 5.121606065272065 0.0 41 0.0 0.0 0.0 5.961153516200699 0.0 42 0.0 0.0 0.0 6.720778982717787 0.0 43 0.0 0.0 0.0 7.577190705586474 0.0 44 0.0 0.0 0.0 8.455599304898705 0.0 45 0.0 0.0 0.0 9.22402352199321 0.0 46 0.0 0.0 0.0 10.112697330312429 0.0 47 0.0 0.0 0.0 10.938313426160152 0.0 48 0.0 0.0 0.0 11.637814097064883 0.0 49 0.0 0.0 0.0 12.373976228708857 0.0 50 0.0 0.0 0.0 13.050013564740473 0.0 51 0.0 0.0 0.0 13.7062537119729 0.0 52 0.0 0.0 0.0 14.399888549159353 0.0 53 0.0 0.0 0.0 15.07739234362054 0.0 54 0.0 0.0 0.0 15.736565407712105 0.0 55 0.0 0.0 0.0 16.375208053789695 0.0 56 0.0 0.0 0.0 17.05124538982131 0.0 57 0.0 0.0 0.0 17.686955119039762 0.0 58 0.0 0.0 0.0 18.302867659459025 0.0 59 0.0 0.0 0.0 18.87551967620598 0.0 60 0.0 0.0 0.0 19.50829648856529 0.0 61 0.0 0.0 0.0 20.119076424481058 0.0 62 0.0 0.0 0.0 20.787048239150042 0.0 63 0.0 0.0 0.0 21.402227550354517 0.0 64 0.0 0.0 0.0 22.040870196432106 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGTAC 15 0.0022206898 70.0 56 ATCGAAT 20 7.90362E-5 70.0 70 ACGAATA 15 0.0022206898 70.0 66 CACGGTT 20 0.0069389683 52.5 49 TTCCGGG 20 0.0069389683 52.5 16 CGTTACT 20 0.0069389683 52.5 2 AATGCAC 20 0.0069389683 52.5 64 AAAGCGC 20 0.0069389683 52.5 8 CATTGAA 30 5.8610906E-4 46.666664 26 CGGACTA 30 5.8610906E-4 46.666664 9 TAAGGAA 35 0.0012518811 40.0 21 AGGGGAG 55 6.655895E-6 38.181816 39 ACTAGCC 40 0.0024119876 35.0 39 CGCTCGG 40 0.0024119876 35.0 10 GCTTCAA 40 0.0024119876 35.0 62 AACTAGC 40 0.0024119876 35.0 38 TTCATAC 55 2.979735E-4 31.81818 3 GTCCTAC 45 0.0042952844 31.111109 1 ATCCCGG 45 0.0042952844 31.111109 11 ATGACAA 45 0.0042952844 31.111109 30 >>END_MODULE