FastQCFastQC Report
Wed 25 May 2016
SRR1780290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136383
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT90776.655521582601938No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC29302.1483615993195633No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG24001.7597501154836013No Hit
CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG20701.517784474604606Illumina PCR Primer Index 10 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC5870.43040554907869744No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT2990.21923553522066533Illumina PCR Primer Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT1760.12904834180213076No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1470.10778469457337059No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAA150.002220689870.033
GTATCTT150.002220689870.043
TAACTCG150.002220689870.036
CTTTATA1000.055.9999962
ACCCTTT200.006938968352.510
TCTTTAT1100.050.909091
TTTATAC1450.043.4482773
GCACCAG350.001251881140.05
TCAGATG350.001251881140.04
TGTATAA400.002411987635.011
GTGTATA400.002411987635.010
ATCTTAC400.002411987635.053
ATAGGGG552.979735E-431.818183
CCTCGCG450.004295284431.11110926
GTCTTGT450.004295284431.1111091
CGTCAGA450.004295284431.1111092
AGTACTT450.004295284431.11110927
TATGCCG21550.030.69605443
TCGTATG21800.030.66513840
CTCGTAT21700.030.6451639