FastQCFastQC Report
Wed 25 May 2016
SRR1780289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549067
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG353.2413538E-431.3858346
ATACCGC353.2413538E-431.3858347
GACTTAC300.00578062829.2934471
TTAAGCC407.06825E-427.47261431
AATTATC450.001403884824.4245530
TATCCAC450.001408362524.4112053
CAGCAAA551.5733832E-424.04180520
TAGCTCG602.864579E-422.00615710
TCTTTCA602.8965433E-421.9700858
ATGCAGT856.1562732E-620.68526331
GCAGCTA654.9242016E-420.3096722
GTGTTAC654.9728656E-420.2800771
AAACCTT550.004435632420.03483819
CTCATTA550.0044732920.00559811
TGCGAGT550.004501704619.98372529
GATACCA550.004515967319.9728055
TACATCG901.012149E-519.5289655
GCAGTGT809.052457E-519.22907633
GTATAGA708.190195E-418.8315011
GATACTC600.007333072418.36526918
Produced by FastQC (version 0.11.5)