FastQCFastQC Report
Wed 25 May 2016
SRR1780289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549067
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT196603.5806194872392623No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC68581.2490278964133703No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG51800.9434185627619216No Hit
CACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTTCTG38140.6946328954389901RNA PCR Primer, Index 34 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC10830.19724368792879554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTACC250.002352179535.1984710
TCTTCTG5000.034.75848844
TCTTTAT1550.034.118961
GTCTTCT5200.033.42162743
ATGCCGT27000.032.509744
CTTTATA1650.032.0015222
CGTCTTC5650.030.75972642
CCGTCTT5750.030.22477341
GGTATTG300.00574353829.33205623
CCAAACG300.00574353829.3320569
ATTTCCA300.00574353829.33205642
GGCCTTA407.0289953E-427.4988049
TATGCCG32750.026.73471643
GCCGTCT6900.025.18731140
AATACCC450.001397596824.4433845
TGCCGTC7550.023.31024639
TAATACC1105.2350515E-922.0010474
TTAATAC500.00257794822.0010473
TATTCCC500.00257794822.0010474
CTCTAGC602.869089E-422.0010472
Produced by FastQC (version 0.11.5)