FastQCFastQC Report
Wed 25 May 2016
SRR1780288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135681
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTC150.002220665370.05
AAGGGTT207.903476E-569.9999942
AAGAGTT200.00693889452.49999621
ATTGGGG200.00693889452.4999963
GCCGTAT200.00693889452.49999654
CTCTTTG305.860984E-446.66666411
TATCCAC501.7025576E-435.0000043
TGCTTGC400.002411944834.99999634
TCAAGGG400.002411944834.9999963
TCGACCA552.979655E-431.818189
CACCGGT450.00429520731.1111097
TTCGAGT450.00429520731.11110930
CCATCTG703.4684315E-530.08
TTCTAGA500.00718832128.00000227
GAGAGAG500.00718832128.0000025
TTGACCC500.00718832128.00000246
AAAGATA500.00718832128.00000225
TTATCCC1253.5015546E-928.02
TCCACAT657.9230045E-426.9230795
CGACGAT808.61548E-526.24999822
Produced by FastQC (version 0.11.5)