##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780288_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 135681 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.907791068756865 34.0 31.0 34.0 12.0 34.0 2 29.934869288994037 34.0 31.0 34.0 12.0 34.0 3 29.734752839380604 34.0 31.0 34.0 11.0 34.0 4 29.6820557041885 34.0 31.0 34.0 11.0 34.0 5 29.64744511022177 34.0 31.0 34.0 11.0 34.0 6 32.43326626425218 37.0 31.0 38.0 11.0 38.0 7 32.87347528393806 37.0 33.0 38.0 12.0 38.0 8 32.76530243733463 37.0 33.0 38.0 11.0 38.0 9 33.08472814911447 38.0 34.0 38.0 12.0 38.0 10-11 32.75635129458067 38.0 33.0 38.0 11.0 38.0 12-13 32.86210302105674 38.0 34.0 38.0 11.0 38.0 14-15 32.75658345678467 38.0 33.0 38.0 11.0 38.0 16-17 32.81074358237336 38.0 33.5 38.0 11.0 38.0 18-19 32.811701712104124 38.0 33.5 38.0 11.0 38.0 20-21 32.811709082332825 38.0 33.5 38.0 11.0 38.0 22-23 32.83346968256425 38.0 33.5 38.0 11.0 38.0 24-25 32.860839026835 38.0 34.0 38.0 11.0 38.0 26-27 32.80841090499038 38.0 34.0 38.0 11.0 38.0 28-29 32.857470832319926 38.0 34.0 38.0 11.0 38.0 30-31 32.897074756229685 38.0 34.0 38.0 11.0 38.0 32-33 32.9370582469174 38.0 34.0 38.0 11.0 38.0 34-35 32.916498993963785 38.0 34.0 38.0 11.0 38.0 36-37 32.840755153632415 38.0 34.0 38.0 11.0 38.0 38-39 32.82713497099815 38.0 34.0 38.0 11.0 38.0 40-41 32.89076952557838 38.0 34.0 38.0 11.0 38.0 42-43 32.96221283746435 38.0 34.0 38.0 11.0 38.0 44-45 33.0410705994207 38.0 34.0 38.0 11.0 38.0 46-47 32.94877691054754 38.0 34.0 38.0 11.0 38.0 48-49 32.88839262682321 38.0 34.0 38.0 11.0 38.0 50-51 32.83360234668082 38.0 34.0 38.0 11.0 38.0 52-53 32.89602449864019 38.0 34.0 38.0 11.0 38.0 54-55 32.84575216868979 38.0 34.0 38.0 11.0 38.0 56-57 32.81242399451655 38.0 33.0 38.0 11.0 38.0 58-59 32.85985510130379 38.0 33.0 38.0 11.0 38.0 60-61 32.880395928685665 38.0 33.0 38.0 11.0 38.0 62-63 32.78329685070128 38.0 33.0 38.0 11.0 38.0 64-65 32.721744385728286 38.0 32.5 38.0 11.0 38.0 66-67 32.65162402989365 38.0 32.0 38.0 11.0 38.0 68-69 32.58329463963267 38.0 32.0 38.0 11.0 38.0 70-71 32.626296238972294 38.0 32.0 38.0 11.0 38.0 72-73 32.60168336023467 38.0 32.0 38.0 11.0 38.0 74-75 32.62108548728267 38.0 32.0 38.0 11.0 38.0 76 31.02570735769931 36.0 26.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03852916358780334 1101 2 0.36258932995317394 1101 3 0.13878632425867465 1101 4 0.20077057949708887 1101 5 0.36669758435208166 1101 6 0.3425264540826163 1101 7 -0.08415267238815005 1101 8 -0.23436310664875748 1101 9 0.27135770524609626 1101 10-11 -0.1406671119112346 1101 12-13 0.33378468334080225 1101 14-15 -0.1467081804364696 1101 16-17 0.15488375956461198 1101 18-19 0.14392925419805636 1101 20-21 0.2939712134263033 1101 22-23 0.24039037167295163 1101 24-25 0.02097735225191144 1101 26-27 0.17419184293943601 1101 28-29 -0.26437855170915014 1101 30-31 -0.17176586657613768 1101 32-33 -0.11305387428617308 1101 34-35 -0.238153779573139 1101 36-37 -0.020953235923720115 1101 38-39 0.20713985999906726 1101 40-41 0.2567395167688318 1101 42-43 -0.06108351788434874 1101 44-45 0.007425913131093864 1101 46-47 0.1018973125387106 1101 48-49 0.11271006004196948 1101 50-51 -0.07195882591122427 1101 52-53 0.25012191070629797 1101 54-55 0.3019211318705288 1101 56-57 0.07161527367433962 1101 58-59 0.5115036652076981 1101 60-61 0.047101100005583874 1101 62-63 0.14680615146664877 1101 64-65 0.3640612978320803 1101 66-67 0.06874179699536853 1101 68-69 -0.18684320514772423 1101 70-71 -0.1740303061794677 1101 72-73 0.39236119755203447 1101 74-75 -0.018262451251743528 1101 76 -0.7595706112145777 1102 1 0.513040891463703 1102 2 0.45038486308091663 1102 3 0.45866297130100975 1102 4 0.4646945118398591 1102 5 -0.17248099708890408 1102 6 0.3033245103390385 1102 7 -0.2099820341300429 1102 8 -0.15264177219403052 1102 9 0.24871963403779773 1102 10-11 -0.06639835198225086 1102 12-13 0.29465282650499347 1102 14-15 0.023544599677535416 1102 16-17 -0.09830519473872101 1102 18-19 -0.2329864546141991 1102 20-21 -0.16092786142650795 1102 22-23 -0.24531189914285534 1102 24-25 -0.5005510095834254 1102 26-27 -0.1978177823292242 1102 28-29 -0.20378841994575936 1102 30-31 0.02159223079794259 1102 32-33 0.01800864323556084 1102 34-35 -0.3369179137662144 1102 36-37 -0.0755392559218464 1102 38-39 -0.3539058366648007 1102 40-41 0.05055551527364344 1102 42-43 0.3370217993757265 1102 44-45 0.12011397966693238 1102 46-47 -0.3149796148512536 1102 48-49 -0.3085356179320513 1102 50-51 -0.20242560471694304 1102 52-53 6.242002119520862E-4 1102 54-55 -0.1828816902956305 1102 56-57 0.11677460457799071 1102 58-59 -0.39803749916946174 1102 60-61 0.09348695179513555 1102 62-63 -0.7454585901462778 1102 64-65 -0.16704560806456215 1102 66-67 -0.2733173563547808 1102 68-69 -0.10030923635975597 1102 70-71 -0.30219589818021575 1102 72-73 -0.19083196196576324 1102 74-75 -0.49299612100223555 1102 76 -0.19850155224812838 1103 1 0.035640192523739955 1103 2 0.10239704003915051 1103 3 0.5995117256330573 1103 4 0.08991883378817178 1103 5 0.2634264987152761 1103 6 -1.0488482339085081 1103 7 -0.23732206624350027 1103 8 -0.05386019456592095 1103 9 0.20397149218640465 1103 10-11 -0.4027468629204449 1103 12-13 0.22852598561191684 1103 14-15 -0.5316077212826755 1103 16-17 -0.29413553105817414 1103 18-19 0.04881277088475855 1103 20-21 -0.3180476765924922 1103 22-23 -0.15512902680475094 1103 24-25 -0.11148774897763047 1103 26-27 -0.5724299868963101 1103 28-29 -0.16174303970642967 1103 30-31 -0.6074573706900352 1103 32-33 -0.3679304574423128 1103 34-35 -0.14452415336747748 1103 36-37 -0.06487715198177568 1103 38-39 -0.5713800623453977 1103 40-41 -0.4380195045248101 1103 42-43 -0.3289219512146033 1103 44-45 -0.581997371205297 1103 46-47 -0.06230409881995058 1103 48-49 -0.3593940353634153 1103 50-51 -0.8906816695832234 1103 52-53 -0.17674089783581337 1103 54-55 -0.5380260192188118 1103 56-57 -0.17336176863560837 1103 58-59 -0.10416303227466273 1103 60-61 -0.2044743522146888 1103 62-63 -0.24801604199426208 1103 64-65 -0.02180123336880513 1103 66-67 -0.31163554114521475 1103 68-69 -0.2709392268419677 1103 70-71 0.13232388937690587 1103 72-73 -0.03473658063661844 1103 74-75 -0.11093679102555853 1103 76 0.027338883099925937 1104 1 0.17157747682955815 1104 2 0.1224715839481263 1104 3 0.1686900715720121 1104 4 0.7953178180729203 1104 5 -0.15351080738700773 1104 6 0.2979044561384967 1104 7 0.7168472341626426 1104 8 -0.11199136568996693 1104 9 -0.11984405159905975 1104 10-11 0.4159864718659847 1104 12-13 0.07378561783290394 1104 14-15 0.3514578788103293 1104 16-17 0.30142380255124834 1104 18-19 0.15183739362428383 1104 20-21 -0.07149696711757514 1104 22-23 0.05685612253736139 1104 24-25 -0.03307133478667623 1104 26-27 0.3916131119797228 1104 28-29 0.29214653940383783 1104 30-31 0.3549255641312712 1104 32-33 0.17925525570168332 1104 34-35 0.06416609707389398 1104 36-37 0.2212087115578285 1104 38-39 0.33444104680403797 1104 40-41 0.14947150443353507 1104 42-43 0.6297047262049915 1104 44-45 0.35724135094064025 1104 46-47 0.5928794637395356 1104 48-49 0.03699283735249992 1104 50-51 0.04418686140771655 1104 52-53 0.2987271812417589 1104 54-55 0.20736221214339423 1104 56-57 0.2793762305942522 1104 58-59 0.24965897779530621 1104 60-61 0.22627827970841707 1104 62-63 0.5377392418320355 1104 64-65 0.1893229556210798 1104 66-67 0.034270719525970605 1104 68-69 0.4213709804424042 1104 70-71 0.3143352671314332 1104 72-73 0.10185096486350176 1104 74-75 0.16689547791375503 1104 76 -0.07926035983620139 1105 1 -0.5952336253473973 1105 2 -0.43354556963000235 1105 3 -0.22587200591689438 1105 4 -0.18918142252388037 1105 5 0.5857830965410429 1105 6 0.1610183070572262 1105 7 0.15264788337792368 1105 8 -0.08961130672454232 1105 9 0.10617518661530312 1105 10-11 -0.35122666729836993 1105 12-13 -1.0653564054554607 1105 14-15 -0.7210576815101106 1105 16-17 -0.6905280125712991 1105 18-19 -0.12141746804520892 1105 20-21 -0.23405392235745381 1105 22-23 -0.40306674592209646 1105 24-25 -0.5253470130379654 1105 26-27 -0.27326300106712154 1105 28-29 -0.6250896796616487 1105 30-31 -0.3021182727906151 1105 32-33 -0.5628454620087879 1105 34-35 -0.01570525109013232 1105 36-37 -0.5455837777956418 1105 38-39 -0.28997078797365106 1105 40-41 -0.5763727732400099 1105 42-43 -0.07679338273214142 1105 44-45 -0.27072553312380165 1105 46-47 -0.12661411855893334 1105 48-49 -0.2475116148348775 1105 50-51 -0.7023585985523795 1105 52-53 -0.3175173465881329 1105 54-55 -0.20531014260212999 1105 56-57 -0.16783780198563392 1105 58-59 -0.38435335133897297 1105 60-61 -0.46475629547250463 1105 62-63 0.0023227612936054243 1105 64-65 -0.6683373380148296 1105 66-67 -0.3876968792708908 1105 68-69 -0.17227013454017737 1105 70-71 -0.053830104082713603 1105 72-73 0.1480293799443615 1105 74-75 -0.20112843584186635 1105 76 0.03694321978201032 1106 1 0.8565368264230528 1106 2 0.5838536977692641 1106 3 0.5428093127644225 1106 4 1.0557514224089637 1106 5 0.7175596533176005 1106 6 0.9576605536994762 1106 7 0.443337749067787 1106 8 0.23374034162710444 1106 9 0.5287331841732978 1106 10-11 0.5121585585868544 1106 12-13 0.6227693576703004 1106 14-15 1.0264240034715684 1106 16-17 0.7141270545837699 1106 18-19 0.6489579898302509 1106 20-21 1.0668160410125083 1106 22-23 0.7850742797189341 1106 24-25 1.147653597462643 1106 26-27 1.2182052529011287 1106 28-29 1.1488877318157549 1106 30-31 1.0682724476001084 1106 32-33 0.9416875050241771 1106 34-35 0.7843557989709211 1106 36-37 1.0214797142678478 1106 38-39 0.7652337297308591 1106 40-41 0.7339904746232406 1106 42-43 0.959989279050518 1106 44-45 0.7440638441655736 1106 46-47 0.6970122550674489 1106 48-49 1.0350280677048005 1106 50-51 0.9510296798358979 1106 52-53 0.990495473924689 1106 54-55 1.012409637617651 1106 56-57 0.9685022468544204 1106 58-59 1.094645427659806 1106 60-61 0.9544286862124736 1106 62-63 0.843836802807644 1106 64-65 1.1561589989815104 1106 66-67 0.6771230230490062 1106 68-69 1.2523330400630002 1106 70-71 0.9056631804105706 1106 72-73 0.869229013643988 1106 74-75 0.878480843767413 1106 76 0.8583412661800587 1107 1 0.3809005756471393 1107 2 0.02286673315101595 1107 3 0.15705512282028877 1107 4 0.018259181564769023 1107 5 -0.07578299100269703 1107 6 -0.5339905946555845 1107 7 0.1358113679380537 1107 8 -0.3155613343505479 1107 9 0.7114302667307157 1107 10-11 0.18362814269610084 1107 12-13 0.9572355277510027 1107 14-15 0.6543336868980205 1107 16-17 0.4847896864894139 1107 18-19 0.2794281946719579 1107 20-21 0.7051074749045014 1107 22-23 0.5071180413576641 1107 24-25 0.7886762541975898 1107 26-27 0.5434768233542613 1107 28-29 0.45437345496907966 1107 30-31 0.4528006909272335 1107 32-33 0.3762545441303189 1107 34-35 0.14081141659971763 1107 36-37 0.4794561264342079 1107 38-39 0.31843826107785134 1107 40-41 0.3540634106877931 1107 42-43 0.2957279506830517 1107 44-45 0.29837017563671253 1107 46-47 0.41391169640822767 1107 48-49 0.6528275711188343 1107 50-51 0.25247288281541813 1107 52-53 0.13730646088929888 1107 54-55 0.37449064320423986 1107 56-57 0.7591835007339895 1107 58-59 0.25994648290500066 1107 60-61 0.4210255204769098 1107 62-63 0.4294668462589186 1107 64-65 0.04459009767796829 1107 66-67 0.21496442590438392 1107 68-69 0.44095811765456716 1107 70-71 0.4150983138680502 1107 72-73 0.3308444698823507 1107 74-75 0.31629896915536904 1107 76 0.6711749098796211 1108 1 -0.32313384147476043 1108 2 -0.525795425037753 1108 3 0.034637567612069375 1108 4 0.16159937562533955 1108 5 0.5749234575650597 1108 6 0.164047134185779 1108 7 -0.4332627433914311 1108 8 -0.9551100738853577 1108 9 -0.567396394158493 1108 10-11 0.14666532990110426 1108 12-13 -0.30415431115087443 1108 14-15 -0.40542041019416786 1108 16-17 -0.16592449974064039 1108 18-19 -0.23775715329071545 1108 20-21 -0.36532846498933935 1108 22-23 -0.08541279322658824 1108 24-25 -0.416731597982114 1108 26-27 -0.15914547039835014 1108 28-29 -0.19926169877826538 1108 30-31 -0.4665767367643099 1108 32-33 -0.2689426888816442 1108 34-35 -0.30236319148768587 1108 36-37 -0.395356235255079 1108 38-39 0.13151181529564582 1108 40-41 -0.2929985989378565 1108 42-43 -0.02781466062322835 1108 44-45 0.03361832246658736 1108 46-47 0.021596742602071117 1108 48-49 -0.2976204395281883 1108 50-51 -0.35494908286030835 1108 52-53 -0.07329723152314216 1108 54-55 -0.3409490881441215 1108 56-57 -0.3958521698891957 1108 58-59 0.2536789764440428 1108 60-61 0.12473108695116508 1108 62-63 0.39140528541768305 1108 64-65 0.30566269242564204 1108 66-67 0.10088033299052768 1108 68-69 -0.019173577357740612 1108 70-71 0.058879554679570845 1108 72-73 -0.23223682199774487 1108 74-75 -0.39470321286540155 1108 76 0.06333511043983009 1109 1 -0.2600687901825651 1109 2 -0.23299611908054985 1109 3 0.26863348858859837 1109 4 -0.05456603790849712 1109 5 -0.3785026177446724 1109 6 -0.4351355390966205 1109 7 -0.020429039699003226 1109 8 -0.685765152878389 1109 9 -0.12459404415393038 1109 10-11 0.12267443160272506 1109 12-13 -0.03651025160930743 1109 14-15 0.22125001079758277 1109 16-17 -0.10124229828558384 1109 18-19 0.4019341803064407 1109 20-21 -0.05273524103876781 1109 22-23 0.14913105627570644 1109 24-25 -0.24100635369730838 1109 26-27 0.46574798794386396 1109 28-29 -0.15943033900231285 1109 30-31 0.2610685403962023 1109 32-33 -0.31696634112967104 1109 34-35 0.13127277088788958 1109 36-37 -0.2736057558176199 1109 38-39 0.09052371752084554 1109 40-41 0.1145612926940558 1109 42-43 -0.15371645965522163 1109 44-45 -0.016604654002925656 1109 46-47 0.26212214517733656 1109 48-49 -0.0895445818678482 1109 50-51 0.17401502782125533 1109 52-53 -0.05927558834637381 1109 54-55 0.10650304421105261 1109 56-57 -0.07168395583178722 1109 58-59 -0.25523247221808987 1109 60-61 0.17947447375826897 1109 62-63 -0.08421570024486158 1109 64-65 -0.0021285468060341373 1109 66-67 0.05598443941042319 1109 68-69 -0.19562178289181986 1109 70-71 0.167323742071126 1109 72-73 -0.2173552354402517 1109 74-75 0.24942101470757905 1109 76 -0.009760076921285332 1110 1 0.517403737289964 1110 2 0.6598610237765925 1110 3 0.7274246973798064 1110 4 0.5992801159376597 1110 5 0.09127760203555013 1110 6 0.5209084169473357 1110 7 0.17462590535594558 1110 8 0.6411579240446912 1110 9 0.5759554063955221 1110 10-11 0.5869601458884333 1110 12-13 0.772555682456634 1110 14-15 0.6086126481602179 1110 16-17 0.5580983610550732 1110 18-19 0.23297813635039688 1110 20-21 0.711001022697495 1110 22-23 0.5241310562757064 1110 24-25 0.4032244155334652 1110 26-27 0.32975897695484946 1110 28-29 0.6152949357229645 1110 30-31 0.5412883206159833 1110 32-33 0.45501168084835086 1110 34-35 0.319459584074707 1110 36-37 0.6096360024241392 1110 38-39 0.48612811312524684 1110 40-41 0.7739019520347199 1110 42-43 0.6402395843008222 1110 44-45 0.9490546866564173 1110 46-47 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2116 20-21 -0.31823705224694976 2116 22-23 -0.5050803832542883 2116 24-25 -0.6286429553228317 2116 26-27 -0.34197095516289266 2116 28-29 -0.9665747181688502 2116 30-31 -0.32945762319090477 2116 32-33 -0.18168507115310462 2116 34-35 -0.3217537732051312 2116 36-37 -0.3870459859476014 2116 38-39 -0.3033912317957643 2116 40-41 -0.3567105102952013 2116 42-43 -0.3380639681024533 2116 44-45 -0.3071018991400578 2116 46-47 -0.40361101363061636 2116 48-49 -0.06354841736752803 2116 50-51 -0.051624983137315894 2116 52-53 -0.42648282709101437 2116 54-55 -0.32155850642666906 2116 56-57 -0.016350784847645627 2116 58-59 -0.20061465236481268 2116 60-61 -0.2229143424117126 2116 62-63 -0.07258363424990932 2116 64-65 -0.31068079787905845 2116 66-67 -0.4545213290593324 2116 68-69 -0.6105863197400154 2116 70-71 0.07550005329453313 2116 72-73 -0.34858031230245246 2116 74-75 -0.14678077519226918 2116 76 -0.32724660701169483 2117 1 -0.03066124120979552 2117 2 -0.0342622442597218 2117 3 -0.3477703973805042 2117 4 0.06686253352007654 2117 5 0.022994435926296575 2117 6 0.3936508918637216 2117 7 0.47751649302914245 2117 8 0.31671135611986045 2117 9 -0.3740445936044807 2117 10-11 0.43432039433564285 2117 12-13 0.12796259438528068 2117 14-15 0.26972110492084767 2117 16-17 0.02131304492193209 2117 18-19 0.37522610577227056 2117 20-21 -0.3257564227556031 2117 22-23 0.1551829753711047 2117 24-25 0.2596705056752029 2117 26-27 -0.21700006110853565 2117 28-29 -0.2461001908895568 2117 30-31 -0.26300774563660667 2117 32-33 -0.0624791616424929 2117 34-35 -0.050185263831011184 2117 36-37 0.14080338576543738 2117 38-39 0.2713327635472851 2117 40-41 0.07789144080680899 2117 42-43 -0.18216047940358493 2117 44-45 -0.06300137322572397 2117 46-47 -0.04537904928038472 2117 48-49 -0.16992362311645337 2117 50-51 0.09559489722720826 2117 52-53 -0.011134960857923204 2117 54-55 0.051358912979971194 2117 56-57 0.016267947337503585 2117 58-59 -0.053663751084151556 2117 60-61 -0.4852452268310046 2117 62-63 -0.4201728590281064 2117 64-65 -0.29909462420690147 2117 66-67 -0.3464880880621024 2117 68-69 0.12974849900020047 2117 70-71 -0.11510976644933635 2117 72-73 -1.3889231602690515 2117 74-75 -0.37284765864804825 2117 76 -0.5475766082160796 2118 1 0.4269125957784148 2118 2 0.38244456268666127 2118 3 1.0024303040180662 2118 4 0.21146894751424483 2118 5 0.24630114991623842 2118 6 0.9092802491967191 2118 7 0.6727757147302427 2118 8 0.3502855311314832 2118 9 0.06210700989406348 2118 10-11 -0.39736930017570415 2118 12-13 0.12140071497513105 2118 14-15 0.5358384835493197 2118 16-17 0.8945246898283372 2118 18-19 0.5141790782656201 2118 20-21 0.4576931061242746 2118 22-23 0.6427394886596502 2118 24-25 0.3594085375339091 2118 26-27 0.2919702350844773 2118 28-29 0.3984301678377875 2118 30-31 0.5460259299454222 2118 32-33 0.675324531263243 2118 34-35 0.5254474737360653 2118 36-37 0.3541975633122334 2118 38-39 0.436229032613916 2118 40-41 0.39764045842628093 2118 42-43 0.5158282813180932 2118 44-45 0.3329972746806362 2118 46-47 0.08192479151059473 2118 48-49 0.5567886767103118 2118 50-51 0.6515884747824572 2118 52-53 0.6566640842259446 2118 54-55 0.7895597833902457 2118 56-57 0.6581321464332959 2118 58-59 0.6442405037854968 2118 60-61 0.2747032245992642 2118 62-63 0.8396741853797209 2118 64-65 0.4739311078250523 2118 66-67 0.7730454396346857 2118 68-69 0.9060826351391285 2118 70-71 0.8290191894808672 2118 72-73 0.41790491257499696 2118 74-75 0.397520364337538 2118 76 0.5637429506633751 2119 1 0.14291834068067288 2119 2 0.8028802464418305 2119 3 -0.2847718081795314 2119 4 0.6739421795377751 2119 5 0.8837100957954753 2119 6 0.6431571122048823 2119 7 0.20589920432836095 2119 8 0.40387308498993946 2119 9 0.5634185332391795 2119 10-11 0.5160056578985532 2119 12-13 0.4649768306299933 2119 14-15 0.8651039444745194 2119 16-17 0.4181708107292934 2119 18-19 0.40909810782434874 2119 20-21 -0.06861906223232239 2119 22-23 0.2826037782440025 2119 24-25 0.5063679534115053 2119 26-27 0.3353021063917936 2119 28-29 0.6085362077253009 2119 30-31 0.5216401958793639 2119 32-33 0.5118571215433505 2119 34-35 0.7575613053622661 2119 36-37 0.24871668575261197 2119 38-39 0.33528043572889743 2119 40-41 0.5617313792446765 2119 42-43 0.32884375299348534 2119 44-45 0.5357673839885848 2119 46-47 0.40151078014119435 2119 48-49 0.5315128268001814 2119 50-51 0.7567081459125973 2119 52-53 0.4805028485559575 2119 54-55 0.22277099454980487 2119 56-57 0.44983213869316074 2119 58-59 0.29041730573256785 2119 60-61 0.43359535287914497 2119 62-63 0.46591153240449756 2119 64-65 0.6328920373295901 2119 66-67 0.28625527476975776 2119 68-69 0.12045005087912841 2119 70-71 -0.17850594446651513 2119 72-73 0.5483219339979755 2119 74-75 0.6410422990279798 2119 76 0.7106765660817302 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 13.0 13 264.0 14 1143.0 15 2323.0 16 3233.0 17 3772.0 18 3815.0 19 3356.0 20 2548.0 21 1976.0 22 1398.0 23 1170.0 24 1110.0 25 1019.0 26 1148.0 27 1167.0 28 1418.0 29 1617.0 30 2015.0 31 2718.0 32 3396.0 33 4415.0 34 6058.0 35 9214.0 36 17322.0 37 58050.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.931899086828665 19.27535911439332 11.626535771404987 29.166206027373033 2 19.732313293681504 18.64520456069752 31.462769289731064 30.15971285588992 3 18.652574789395715 20.682335772879032 29.512606776188267 31.15248266153699 4 15.016103949705558 21.07000980240417 29.21337549104149 34.700510756848786 5 15.956545131595433 31.40233341440585 32.74592610608707 19.895195347911645 6 29.556828148377445 30.2039342280791 16.715678687509676 23.523558936033783 7 27.89852669128323 27.55949617116619 18.83019730102225 25.711779836528326 8 23.918779481132074 29.388266509433965 18.317364386792452 28.375589622641513 9 23.351267688679243 16.565448113207548 19.16568396226415 40.91760023584906 10-11 26.654161799226863 21.897398668194768 23.97728487144431 27.47115466113406 12-13 24.768481837847002 19.453421825538676 24.014504663529397 31.763591673084928 14-15 24.597769768795928 21.023208850170622 21.779762826040493 32.59925855499296 16-17 26.296607483730224 22.253300019899616 22.22824124232575 29.221851254044413 18-19 25.122161540672604 21.524752913082892 23.10972059463005 30.24336495161445 20-21 24.71606193940198 22.087101362755288 23.26375837442236 29.933078323420375 22-23 26.61057922627339 21.727802713718205 22.28757158334623 29.37404647666217 24-25 25.43069932672713 23.184245341077016 21.685135299471924 29.699920032723938 26-27 24.446680080482896 23.49186695263154 23.310190815220995 28.751262151664562 28-29 25.204339590657497 22.484725201022986 24.624302592109434 27.686632616210083 30-31 25.43392221460632 21.63346378638129 24.35418371032053 28.57843028869186 32-33 24.019206815987502 23.030122124689527 24.46326309505384 28.487407964269128 34-35 24.331057152649056 22.736502297677262 24.385597046001454 28.546843503672232 36-37 25.0057119272411 21.53175463034618 24.626513660718892 28.836019781693828 38-39 23.743647760732014 21.468818290026938 26.627997390929426 28.159536558311622 40-41 23.52871809612252 21.14371208938613 26.9370803576035 28.390489456887845 42-43 22.240033608242864 21.18903899588004 26.942976540562054 29.62795085531504 44-45 21.325388226796676 21.143343577951224 28.27956751497999 29.25170068027211 46-47 21.327599295406134 22.241507653982502 28.352901290527043 28.077991760084313 48-49 21.418621619828862 22.579432639794813 27.69768795925738 28.304257781118945 50-51 21.40314413956265 23.661750724124968 26.155836115594667 28.779269020717713 52-53 20.384947044906802 23.390157796596426 26.6971794134772 29.527715745019567 54-55 20.64548462938805 22.945364494660268 26.78009448633191 29.62905638961977 56-57 21.02726247595463 23.36841562193675 26.36736167923291 29.23696022287572 58-59 21.452524671840568 23.00543185855057 25.79506342081795 29.746980048790917 60-61 21.019523735821522 22.28757158334623 25.963841658006647 30.7290630228256 62-63 20.85848423876593 22.684089887309202 25.931412651734583 30.526013222190286 64-65 21.428202917136517 23.037492353387726 24.68731804747901 30.84698668199674 66-67 21.267163420080927 24.252843065720327 23.479706075279516 31.000287438919226 68-69 20.98083003515599 25.074623565569237 23.570728399702244 30.373817999572527 70-71 21.565289170922973 25.739786705581473 23.036755330517906 29.658168792977648 72-73 21.839461678495883 25.38748977380768 23.309453792351174 29.463594755345255 74-75 21.258687657078 26.371046793582003 24.256159668634517 28.114105880705477 76 21.703112447579247 25.615229840581954 24.239945165498487 28.441712546340312 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.0 15 5.0 16 4.5 17 3.0 18 6.0 19 9.0 20 14.5 21 20.0 22 30.0 23 57.5 24 75.0 25 75.0 26 132.0 27 272.5 28 394.5 29 433.0 30 564.0 31 793.0 32 1113.0 33 1335.0 34 1568.5 35 2040.5 36 2502.5 37 2726.0 38 3124.5 39 4024.5 40 4974.0 41 5657.5 42 5893.0 43 6085.5 44 6524.0 45 6788.0 46 6806.0 47 6722.0 48 6438.0 49 5882.0 50 5526.0 51 5272.5 52 4627.0 53 3871.0 54 3507.0 55 3512.5 56 3417.0 57 3266.0 58 3216.0 59 3250.0 60 3389.5 61 3626.5 62 3758.0 63 3855.0 64 3977.5 65 4012.0 66 3971.0 67 3921.0 68 3827.0 69 3648.5 70 3375.5 71 3187.0 72 2861.5 73 2270.5 74 1670.5 75 1336.0 76 1088.5 77 589.0 78 231.5 79 126.0 80 88.5 81 37.0 82 17.0 83 11.0 84 11.0 85 14.0 86 12.5 87 8.0 88 8.5 89 6.5 90 7.0 91 5.5 92 1.0 93 2.0 94 4.0 95 4.5 96 4.0 97 6.0 98 8.5 99 19.5 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 7.370228698196505E-4 9 7.370228698196505E-4 10-11 3.6851143490982524E-4 12-13 3.6851143490982524E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.6851143490982524E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.6851143490982524E-4 36-37 0.0 38-39 3.6851143490982524E-4 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 135681.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.58047184204126 #Duplication Level Percentage of deduplicated Percentage of total 1 75.31746316709155 46.38084919775061 2 12.735628882266345 15.685320715501803 3 5.171567747417806 9.55402746147213 4 2.2847773269661174 5.627906633942851 5 1.2818211195289217 3.9467574678842285 6 0.7671777195313155 2.8345899573263758 7 0.6103910092994866 2.631171645256152 8 0.39974626883535 1.969325108158106 9 0.31477026557993126 1.7445331328631126 >10 1.1166564934831784 9.625518679844635 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009581297307655456 0.0 2 0.0 0.0 0.0 0.011055343047294757 0.0 3 0.0 0.0 0.0 0.011792365917114408 0.0 4 0.0 0.0 0.0 0.02874389192296637 0.0 5 0.0 0.0 0.0 0.033903052011703926 0.0 6 0.0 0.0 0.0 0.03537709775134323 0.0 7 0.0 0.0 0.0 0.03537709775134323 0.0 8 0.0 0.0 0.0 0.03611412062116288 0.0 9 0.0 0.0 0.0 0.042010303579720076 0.0 10 0.0 0.0 0.0 0.04790648653827728 0.0 11 0.0 0.0 0.0 0.04864350940809693 0.0 12 0.0 0.0 0.0 0.04938053227791658 0.0 13 0.0 0.0 0.0 0.052328623757195186 0.0 14 0.0 0.0 0.0 0.05601373810629344 0.0 15 0.0 0.0 0.0 0.05675076097611309 0.0 16 0.0 0.0 0.0 0.05748778384593274 0.0 17 0.0 0.0 0.0 0.061909921064850645 0.0 18 0.0 0.0 0.0 0.0641209896743096 0.0 19 0.0 0.0 0.0 0.0685431268932275 7.370228698196505E-4 20 0.0 0.0 0.0 0.07222824124232576 7.370228698196505E-4 21 0.0 0.0 0.0 0.0773874013310633 7.370228698196505E-4 22 0.0 0.0 0.0 0.0862316757688991 7.370228698196505E-4 23 0.0 0.0 0.0 0.09139083585763666 7.370228698196505E-4 24 0.0 0.0 0.0 0.10244617890493142 7.370228698196505E-4 25 0.0 0.0 0.0 0.10834236186348863 7.370228698196505E-4 26 0.0 0.0 0.0 0.12381984212970129 7.370228698196505E-4 27 0.0 0.0 0.0 0.21373663224769865 7.370228698196505E-4 28 0.0 0.0 0.0 0.3132347196733515 7.370228698196505E-4 29 0.0 0.0 0.0 0.4274732644953973 7.370228698196505E-4 30 0.0 0.0 0.0 0.5512931066250986 7.370228698196505E-4 31 0.0 0.0 0.0 0.6913274518908322 7.370228698196505E-4 32 0.0 0.0 0.0 0.8770572150853841 7.370228698196505E-4 33 0.0 0.0 0.0 1.0863717101141648 7.370228698196505E-4 34 0.0 0.0 0.0 1.3797068123023857 7.370228698196505E-4 35 0.0 0.0 0.0 1.7887545050522917 7.370228698196505E-4 36 0.0 0.0 0.0 2.3503659318548653 7.370228698196505E-4 37 0.0 0.0 0.0 3.039482315136239 7.370228698196505E-4 38 0.0 0.0 0.0 3.7927196880919216 7.370228698196505E-4 39 0.0 0.0 0.0 4.5599604955741775 0.0022110686094589517 40 0.0 0.0 0.0 5.33457153175463 0.0022110686094589517 41 0.0 0.0 0.0 6.05316882982879 0.0022110686094589517 42 0.0 0.0 0.0 6.822620705920505 0.0022110686094589517 43 0.0 0.0 0.0 7.538269912515386 0.0022110686094589517 44 0.0 0.0 0.0 8.264237439287742 0.0022110686094589517 45 0.0 0.0 0.0 9.085280916266832 0.0022110686094589517 46 0.0 0.0 0.0 9.878317524192775 0.0022110686094589517 47 0.0 0.0 0.0 10.62492169132008 0.0022110686094589517 48 0.0 0.0 0.0 11.330252577737488 0.0022110686094589517 49 0.0 0.0 0.0 11.985465909007157 0.0022110686094589517 50 0.0 0.0 0.0 12.556658633117385 0.0022110686094589517 51 0.0 0.0 0.0 13.155858226280762 0.0022110686094589517 52 0.0 0.0 0.0 13.788223848586021 0.0022110686094589517 53 0.0 0.0 0.0 14.430907791068757 0.0022110686094589517 54 0.0 0.0 0.0 15.041899750149247 0.0022110686094589517 55 0.0 0.0 0.0 15.635940183223886 0.0022110686094589517 56 0.0 0.0 0.0 16.245458096564736 0.0022110686094589517 57 0.0 0.0 0.0 16.838024483899737 0.0022110686094589517 58 0.0 0.0 0.0 17.44017216854239 0.0022110686094589517 59 0.0 0.0 0.0 18.006942755433702 0.0022110686094589517 60 0.0 0.0 0.0 18.58034654815339 0.0022110686094589517 61 0.0 0.0 0.0 19.195760644452797 0.0022110686094589517 62 0.0 0.0 0.0 19.749264819687355 0.0022110686094589517 63 0.0 0.0 0.0 20.30424304066155 0.0022110686094589517 64 0.0 0.0 0.0 20.868065536073583 0.0022110686094589517 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTAGTC 15 0.0022206653 70.0 5 AAGGGTT 20 7.903476E-5 69.99999 42 AAGAGTT 20 0.006938894 52.499996 21 ATTGGGG 20 0.006938894 52.499996 3 GCCGTAT 20 0.006938894 52.499996 54 CTCTTTG 30 5.860984E-4 46.666664 11 TATCCAC 50 1.7025576E-4 35.000004 3 TGCTTGC 40 0.0024119448 34.999996 34 TCAAGGG 40 0.0024119448 34.999996 3 TCGACCA 55 2.979655E-4 31.81818 9 CACCGGT 45 0.004295207 31.111109 7 TTCGAGT 45 0.004295207 31.111109 30 CCATCTG 70 3.4684315E-5 30.0 8 TTCTAGA 50 0.007188321 28.000002 27 GAGAGAG 50 0.007188321 28.000002 5 TTGACCC 50 0.007188321 28.000002 46 AAAGATA 50 0.007188321 28.000002 25 TTATCCC 125 3.5015546E-9 28.0 2 TCCACAT 65 7.9230045E-4 26.923079 5 CGACGAT 80 8.61548E-5 26.249998 22 >>END_MODULE