FastQCFastQC Report
Wed 25 May 2016
SRR1780288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135681
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT107377.913414553253588No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC35442.6120090506408413No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG28672.113044567772938No Hit
CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG27282.0105983888680066RNA PCR Primer, Index 34 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC6580.4849610483413301No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT3650.26901334748417244RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT3070.22626602103463267No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1480.10907938473330826No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1470.10834236186348861No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT1380.10170915603511177No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT207.903476E-569.9999937
TCTATCG207.903476E-569.9999929
CTTTATA850.061.7647062
ATCGTAT252.3835005E-456.00000439
TATCGCC252.3835005E-456.00000431
GCATATC252.3835005E-456.00000435
TTTATAC1000.052.5000043
CAAACGC200.00693889452.49999619
CCCACGT200.00693889452.49999659
TTCTATC305.860984E-446.66666428
GTTTGGC305.860984E-446.66666459
CATATCG305.860984E-446.66666436
CTATATC305.860984E-446.6666642
TATTGCG305.860984E-446.6666649
AAATTCA305.860984E-446.66666470
TAAATGG305.860984E-446.66666453
ACTTTAT305.860984E-446.66666441
TATCGTA305.860984E-446.66666438
TCTTTAT1150.045.6521721
GTTGTCT350.001251858140.070
Produced by FastQC (version 0.11.5)