##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780288_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 135681 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.73820947664006 34.0 34.0 34.0 34.0 34.0 2 33.74123864063502 34.0 34.0 34.0 34.0 34.0 3 33.74527752596163 34.0 34.0 34.0 34.0 34.0 4 33.77911424591505 34.0 34.0 34.0 34.0 34.0 5 33.76232486494056 34.0 34.0 34.0 34.0 34.0 6 37.516240298936474 38.0 38.0 38.0 37.0 38.0 7 37.5259616305894 38.0 38.0 38.0 37.0 38.0 8 37.43967836321961 38.0 38.0 38.0 37.0 38.0 9 37.45060104215034 38.0 38.0 38.0 37.0 38.0 10-11 37.40002653282332 38.0 38.0 38.0 37.0 38.0 12-13 37.38082708706452 38.0 38.0 38.0 37.0 38.0 14-15 37.31769370803576 38.0 38.0 38.0 36.0 38.0 16-17 37.33710689042681 38.0 38.0 38.0 36.5 38.0 18-19 37.2878995585233 38.0 38.0 38.0 36.0 38.0 20-21 37.323829423427014 38.0 38.0 38.0 36.5 38.0 22-23 37.31267826740664 38.0 38.0 38.0 36.0 38.0 24-25 37.31034927513801 38.0 38.0 38.0 36.0 38.0 26-27 37.196995157759744 38.0 38.0 38.0 36.0 38.0 28-29 37.167215748704685 38.0 38.0 38.0 36.0 38.0 30-31 37.14777308539884 38.0 38.0 38.0 36.0 38.0 32-33 37.07567382315874 38.0 38.0 38.0 36.0 38.0 34-35 37.14695867512769 38.0 38.0 38.0 36.0 38.0 36-37 37.158563100213 38.0 38.0 38.0 36.0 38.0 38-39 36.419015927064216 38.0 37.0 38.0 34.0 38.0 40-41 36.72657188552561 38.0 37.0 38.0 34.5 38.0 42-43 37.02495559437209 38.0 38.0 38.0 36.0 38.0 44-45 37.11752198170709 38.0 38.0 38.0 36.0 38.0 46-47 37.05600268276325 38.0 38.0 38.0 36.0 38.0 48-49 37.08019545846508 38.0 38.0 38.0 36.0 38.0 50-51 36.91756030689632 38.0 38.0 38.0 35.0 38.0 52-53 36.89923791835261 38.0 38.0 38.0 35.0 38.0 54-55 36.87873025699987 38.0 37.5 38.0 35.5 38.0 56-57 36.92050471326125 38.0 38.0 38.0 35.0 38.0 58-59 36.8097780824139 38.0 37.5 38.0 35.0 38.0 60-61 36.80135759612621 38.0 37.0 38.0 35.0 38.0 62-63 36.78307942895468 38.0 37.0 38.0 35.0 38.0 64-65 36.70523875855868 38.0 37.5 38.0 34.5 38.0 66-67 36.34564898548802 38.0 37.0 38.0 34.0 38.0 68-69 36.214289399400066 38.0 37.0 38.0 34.0 38.0 70-71 35.42776807364332 38.0 36.5 38.0 29.0 38.0 72-73 34.02063664035495 38.0 35.5 38.0 21.5 38.0 74-75 33.57158703134559 38.0 35.0 38.0 19.0 38.0 76 32.91058438543348 38.0 33.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03383183504295317 1101 2 0.05287913041772896 1101 3 0.10625025388768705 1101 4 0.07318138730465762 1101 5 0.09516067700279507 1101 6 -0.046458208252524 1101 7 0.14771379238448645 1101 8 -0.004806228107334221 1101 9 0.04071608166915297 1101 10-11 0.056526128304653867 1101 12-13 0.002382639004700593 1101 14-15 0.2216399952628123 1101 16-17 0.1125076756189074 1101 18-19 0.11171606781481813 1101 20-21 0.06108633208626202 1101 22-23 0.048050569413227606 1101 24-25 0.11282102809666839 1101 26-27 0.07457153054969723 1101 28-29 -0.01298289868229574 1101 30-31 0.03942018062898711 1101 32-33 0.02637270912331502 1101 34-35 0.057554071460067746 1101 36-37 0.16230380614543094 1101 38-39 0.1334711630623815 1101 40-41 0.17379483096284787 1101 42-43 0.089938430884267 1101 44-45 0.2212190460784953 1101 46-47 0.42956490755119603 1101 48-49 0.09623463250470365 1101 50-51 0.07378847962238666 1101 52-53 0.14284203334132428 1101 54-55 0.19738532983208756 1101 56-57 0.0318031275549302 1101 58-59 0.04258329791309734 1101 60-61 -0.03161195362509517 1101 62-63 0.13595559779160737 1101 64-65 -0.023604116554004406 1101 66-67 0.10321855044431061 1101 68-69 -0.2005080180858343 1101 70-71 0.40098742770794615 1101 72-73 0.039151101260365806 1101 74-75 0.2207283495751824 1101 76 -0.5790907786418202 1102 1 -0.05080978723534457 1102 2 0.042482905765830026 1102 3 -0.11066872564693853 1102 4 0.11100495519550435 1102 5 -0.10960990518578484 1102 6 0.05121957887017459 1102 7 -0.06990605622779356 1102 8 0.0543471220468561 1102 9 0.026301540972021087 1102 10-11 -0.026678712364436308 1102 12-13 0.07930302027748581 1102 14-15 0.06381596012590762 1102 16-17 0.09442525341537333 1102 18-19 0.0165964528255671 1102 20-21 -0.2724455808190811 1102 22-23 0.08783657072151385 1102 24-25 -0.039419416721571565 1102 26-27 0.04231987182621566 1102 28-29 0.05403186629293799 1102 30-31 -0.048281903288405204 1102 32-33 -0.05679708809189776 1102 34-35 0.2258327370053408 1102 36-37 0.03214073727336597 1102 38-39 0.17192551312312787 1102 40-41 -0.09829422613462668 1102 42-43 0.1298762871593837 1102 44-45 -0.17620876440650335 1102 46-47 0.0052354056354886325 1102 48-49 0.035878123767204784 1102 50-51 0.062294226748825565 1102 52-53 0.0929635173147858 1102 54-55 0.05791238337847915 1102 56-57 0.20724233884359222 1102 58-59 -0.46714710111503166 1102 60-61 -0.3119479035363213 1102 62-63 0.002369951590324604 1102 64-65 0.0541339453093741 1102 66-67 -0.07116935722787332 1102 68-69 -0.05156165793258083 1102 70-71 -0.3129178147927618 1102 72-73 -0.3926076128784999 1102 74-75 -0.7447188048963582 1102 76 -0.47255867883806246 1103 1 7.897099928726448E-4 1103 2 0.023272954953185376 1103 3 -0.15508992052912873 1103 4 -0.07555825091912993 1103 5 -0.011823699441691815 1103 6 -0.29003313660604135 1103 7 -0.07487565015965458 1103 8 -0.1761722877048797 1103 9 -0.04851845655770859 1103 10-11 0.021792992191748795 1103 12-13 -0.07043869161685024 1103 14-15 -0.0061688550037501955 1103 16-17 -0.07628465743709256 1103 18-19 0.1928499311780456 1103 20-21 -0.19632869364484407 1103 22-23 -0.06888727533731753 1103 24-25 -0.0459837853143199 1103 26-27 -0.021754468649092473 1103 28-29 -0.04914896444298478 1103 30-31 -0.056016015482072135 1103 32-33 -0.025936926943366245 1103 34-35 -0.09189432766476813 1103 36-37 -0.1309113490115763 1103 38-39 -0.03986710078157074 1103 40-41 0.05959573078103375 1103 42-43 -0.23497836674908257 1103 44-45 -0.0026762113865999027 1103 46-47 0.05825893855178066 1103 48-49 -0.08068210349727423 1103 50-51 -0.1481615373262386 1103 52-53 -0.04101208517582222 1103 54-55 -0.19577666825734497 1103 56-57 -0.2417285047522526 1103 58-59 -0.004175661749158621 1103 60-61 0.14646807472534107 1103 62-63 -0.07869110030706139 1103 64-65 0.09399241359914612 1103 66-67 -0.6576940562550249 1103 68-69 -0.461451440476516 1103 70-71 -0.26826775703213457 1103 72-73 -0.24758120282887575 1103 74-75 -0.2254289337134665 1103 76 -0.5237149812858064 1104 1 0.08198154067793695 1104 2 -0.07946831374636787 1104 3 0.06277300877040659 1104 4 -0.021786372717770064 1104 5 0.03673155822885121 1104 6 0.09729003957478 1104 7 0.09269556978846794 1104 8 1.4658004143797143E-4 1104 9 -0.03873910943448067 1104 10-11 0.0993375477981644 1104 12-13 0.09014312867856233 1104 14-15 -0.1109807878415694 1104 16-17 -0.01804087124587994 1104 18-19 0.08570214388247166 1104 20-21 0.12863843002102726 1104 22-23 -0.12896559730016577 1104 24-25 0.006651043983033844 1104 26-27 0.1425908745362463 1104 28-29 -0.18038547788049897 1104 30-31 -0.2569539900092721 1104 32-33 -0.12590277914880943 1104 34-35 -0.0018095394174224566 1104 36-37 0.015880574671740533 1104 38-39 0.08791467301472977 1104 40-41 0.05346729148055829 1104 42-43 0.009280081197324819 1104 44-45 -0.06374263974525718 1104 46-47 -0.21292177593633 1104 48-49 -0.0806530415444513 1104 50-51 0.004028644092997524 1104 52-53 -0.0344077563979468 1104 54-55 -0.2537407331526893 1104 56-57 -0.2670124037038377 1104 58-59 0.07079327828876814 1104 60-61 -0.02875007155800091 1104 62-63 0.10670599495075805 1104 64-65 0.12052960926601486 1104 66-67 0.0629769842355401 1104 68-69 0.15846544233841797 1104 70-71 -0.10289071452176302 1104 72-73 0.44479076110523863 1104 74-75 0.4409180514722024 1104 76 0.3810998577473015 1105 1 -0.2803654619129645 1105 2 0.03137477270647793 1105 3 0.03826364279762373 1105 4 0.0216782926981125 1105 5 0.12708564858294125 1105 6 0.045337926647057714 1105 7 0.09305293599949493 1105 8 0.1435769978620982 1105 9 0.043842265829823646 1105 10-11 0.06061542980775414 1105 12-13 0.0609433311860883 1105 14-15 0.13388579543964596 1105 16-17 0.07407771477515013 1105 18-19 -0.009305152344332157 1105 20-21 0.11666293938699823 1105 22-23 0.17114012353726338 1105 24-25 -0.047151183599680735 1105 26-27 -0.07684314672705028 1105 28-29 0.08901280676168 1105 30-31 0.10276488190471866 1105 32-33 0.003605246700679743 1105 34-35 0.013992747726327082 1105 36-37 0.08440182307103328 1105 38-39 -0.42251595326955993 1105 40-41 -0.08934145803551274 1105 42-43 -0.05353447551967605 1105 44-45 -0.249792700795318 1105 46-47 -0.2330124576123751 1105 48-49 -0.3017696620513135 1105 50-51 -0.24539808094348103 1105 52-53 0.10660671693359802 1105 54-55 -0.11370157177206153 1105 56-57 -0.004069046248282859 1105 58-59 0.21592863074118895 1105 60-61 0.0919354759568094 1105 62-63 0.018976312708872456 1105 64-65 0.031925124320061116 1105 66-67 -0.23300413491630678 1105 68-69 0.31138095501002994 1105 70-71 -0.042157071471045526 1105 72-73 -0.11477399663025523 1105 74-75 -0.4157185368717009 1105 76 -0.2538728930791123 1106 1 -0.10860111514862325 1106 2 -0.0960672297355245 1106 3 -0.08905625952227325 1106 4 -0.058236237216419795 1106 5 0.008953780451072646 1106 6 0.021070527379656312 1106 7 -0.008320690374134188 1106 8 0.06674549603059887 1106 9 0.16878121576877447 1106 10-11 0.0627521819445036 1106 12-13 0.1992217194644752 1106 14-15 0.16273194928579926 1106 16-17 0.13544828864572622 1106 18-19 0.22445553141635344 1106 20-21 0.21295414817821268 1106 22-23 0.06222681462395485 1106 24-25 0.16234210089360346 1106 26-27 -0.0326123774962781 1106 28-29 0.15047495572382985 1106 30-31 0.17773435687419692 1106 32-33 0.25424627234171027 1106 34-35 0.19361728985087012 1106 36-37 0.28864480231401046 1106 38-39 0.1861531553995448 1106 40-41 0.27494425625020824 1106 42-43 0.22801314352795288 1106 44-45 0.11542402692140996 1106 46-47 0.25106873896882576 1106 48-49 0.16108137579972492 1106 50-51 0.21646089341549413 1106 52-53 0.21501636284324377 1106 54-55 0.2097416516711661 1106 56-57 0.19210006242082756 1106 58-59 0.1354951753077387 1106 60-61 0.2853420693634021 1106 62-63 0.2725935288260928 1106 64-65 0.1306583355532709 1106 66-67 0.16456912515695166 1106 68-69 0.2956605642896406 1106 70-71 0.5326445158305404 1106 72-73 0.6880495686933088 1106 74-75 0.9248950162418552 1106 76 0.829338340132125 1107 1 -0.21401700589970574 1107 2 0.058417562319100114 1107 3 -0.037861349528483856 1107 4 -0.06255032784335413 1107 5 0.06621635028347583 1107 6 0.21404073222137043 1107 7 0.1035508180057576 1107 8 0.23997640850732438 1107 9 0.09123621266510895 1107 10-11 0.035905502863187166 1107 12-13 0.016726374957954704 1107 14-15 0.15714535710702648 1107 16-17 0.00952494910506374 1107 18-19 -0.21536445616899869 1107 20-21 0.04961615935139463 1107 22-23 -0.028058722309623363 1107 24-25 0.14384427345722628 1107 26-27 0.12465019233612651 1107 28-29 0.25434677446995124 1107 30-31 0.08456241398158681 1107 32-33 0.06881796694133158 1107 34-35 -0.013947887057874198 1107 36-37 0.13758955712469145 1107 38-39 0.3936329815944575 1107 40-41 0.11865934004908496 1107 42-43 0.0380457319823293 1107 44-45 0.14187498905673124 1107 46-47 0.0809647662810491 1107 48-49 -0.08196642060003256 1107 50-51 -0.048040968781897675 1107 52-53 0.006163166071054604 1107 54-55 0.17654798314229936 1107 56-57 -0.03778044657979507 1107 58-59 0.2286826365119552 1107 60-61 -0.2057193831505586 1107 62-63 -0.00629380823163217 1107 64-65 0.2667461691969777 1107 66-67 0.4274415646355365 1107 68-69 0.2862719857669802 1107 70-71 0.34022521105090675 1107 72-73 0.6241218840254064 1107 74-75 0.6685868225733245 1107 76 0.4819764531054318 1108 1 -0.11311788954073876 1108 2 -0.10341564463241326 1108 3 0.1334814443866179 1108 4 0.12811340882851852 1108 5 -0.04021519461817746 1108 6 -0.046911928760692945 1108 7 0.10379842319927235 1108 8 0.06533737045349852 1108 9 0.13936447215534997 1108 10-11 -0.031276750034564316 1108 12-13 -0.1458844670544579 1108 14-15 -0.11012123630976589 1108 16-17 0.08985349024622025 1108 18-19 0.11220560836310511 1108 20-21 0.14366744457710467 1108 22-23 0.0446675656242661 1108 24-25 0.21791304579972604 1108 26-27 0.20796933995524824 1108 28-29 0.0304110899189709 1108 30-31 0.05772820113396904 1108 32-33 0.10934784205546322 1108 34-35 0.10540168504939373 1108 36-37 0.14747828954085662 1108 38-39 -0.00952243709044609 1108 40-41 -0.15171779361129722 1108 42-43 0.09990834205057553 1108 44-45 0.20064640303714043 1108 46-47 0.0798737558971041 1108 48-49 0.07591670974863263 1108 50-51 0.06506430985131573 1108 52-53 0.16899066262779172 1108 54-55 0.1569484845428164 1108 56-57 0.16827350040174593 1108 58-59 0.05258504911506634 1108 60-61 0.431879465485288 1108 62-63 -0.06142078326993783 1108 64-65 0.11719650114269342 1108 66-67 0.3098484418697893 1108 68-69 0.06278121679810766 1108 70-71 0.2056169688930254 1108 72-73 -0.2726344879394986 1108 74-75 -0.14053665504162183 1108 76 0.1594706885470245 1109 1 0.03831585676834948 1109 2 0.028627519959222525 1109 3 0.06298891752290103 1109 4 0.0546453256651489 1109 5 0.03917356067085365 1109 6 -0.21565108434194968 1109 7 0.03810788105443663 1109 8 -0.08719223290712819 1109 9 -0.18143245944490616 1109 10-11 -0.30202193282961076 1109 12-13 -0.042078646835896905 1109 14-15 0.050094586648441464 1109 16-17 0.09330848400592373 1109 18-19 0.15415638611720794 1109 20-21 0.11254206026612223 1109 22-23 -0.025288447891313126 1109 24-25 0.18636529991680106 1109 26-27 0.02205795217405182 1109 28-29 0.023078740827621402 1109 30-31 0.036830815970652964 1109 32-33 0.08190194999738054 1109 34-35 0.07443230816588908 1109 36-37 0.05555566922487998 1109 38-39 0.09396756321395827 1109 40-41 0.20873546504141416 1109 42-43 3.695305176165675E-5 1109 44-45 0.14443806843544849 1109 46-47 0.1021523775524571 1109 48-49 0.183120447838796 1109 50-51 0.33976675422135116 1109 52-53 0.15056276088964182 1109 54-55 0.16926546119496777 1109 56-57 0.18411776693852744 1109 58-59 0.18158797140053196 1109 60-61 0.0672102012315392 1109 62-63 -0.03734236860980644 1109 64-65 0.10335369574863762 1109 66-67 0.07743542552324811 1109 68-69 -0.14054212191305027 1109 70-71 0.21471106039709298 1109 72-73 -0.10790586476212383 1109 74-75 0.00461113345796349 1109 76 0.5043688651626468 1110 1 -0.12651930806681122 1110 2 -0.11972412839242708 1110 3 -0.12931877478479237 1110 4 0.1535464245662439 1110 5 0.08862411012140115 1110 6 -0.2568598755507452 1110 7 -0.11573827279171667 1110 8 0.03643414071924411 1110 9 0.019116991104546344 1110 10-11 -0.12207687788455246 1110 12-13 -0.0146061193633642 1110 14-15 -0.16419112763728094 1110 16-17 -0.02207613137870368 1110 18-19 -0.07386559190477726 1110 20-21 -0.15943596171189967 1110 22-23 -0.20248625008910892 1110 24-25 -0.047151183599680735 1110 26-27 -0.04250248738638618 1110 28-29 0.0189578617067383 1110 30-31 -0.04558676644693094 1110 32-33 -0.019746401650969858 1110 34-35 -0.282710548976965 1110 36-37 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0.2176950105398774 2115 62-63 0.14783862686530824 2115 64-65 0.22828905450752046 2115 66-67 0.19275539966675126 2115 68-69 0.43503964925696437 2115 70-71 0.05364124785668878 2115 72-73 0.18698747719004416 2115 74-75 0.5746499182026312 2115 76 0.6012337649687254 2116 1 -0.05217606128589125 2116 2 -0.03970373327507559 2116 3 0.0420687020043502 2116 4 0.03393058334375354 2116 5 0.045657381559102816 2116 6 0.014029899494154563 2116 7 -0.02916556572039042 2116 8 0.20384152557815582 2116 9 0.0839628101054899 2116 10-11 0.1598589840374558 2116 12-13 -0.028155935198505233 2116 14-15 0.20982336202818175 2116 16-17 -0.1137476177870198 2116 18-19 0.07619452803069748 2116 20-21 0.1879887742169899 2116 22-23 0.1374653495577789 2116 24-25 0.024007228625208654 2116 26-27 -0.049648388576606806 2116 28-29 0.004095300445293049 2116 30-31 0.09235423991513869 2116 32-33 -0.04023664547916184 2116 34-35 0.09155126953692871 2116 36-37 0.12393258316966183 2116 38-39 0.04172410030567164 2116 40-41 0.018642317192032465 2116 42-43 0.005725516565810551 2116 44-45 0.024746126028830417 2116 46-47 0.15189591656192647 2116 48-49 -0.054277196068547084 2116 50-51 0.022128021762675587 2116 52-53 -0.015219863687995883 2116 54-55 0.21373518814392867 2116 56-57 0.2790768245014661 2116 58-59 0.31989806850564406 2116 60-61 -0.21077981429309744 2116 62-63 0.16849611423698718 2116 64-65 0.038249132721638546 2116 66-67 0.17549941139887437 2116 68-69 0.2068255381277453 2116 70-71 0.3524313733251603 2116 72-73 -0.14798074832730634 2116 74-75 0.1184661150414783 2116 76 0.17332110405948953 2117 1 -0.002924786646204325 2117 2 0.10332890553017648 2117 3 -0.04859732232420555 2117 4 0.0922468545710231 2117 5 0.02996827320469464 2117 6 0.19244733897386368 2117 7 0.10725901977079388 2117 8 -0.41550088078099634 2117 9 0.0507860466866461 2117 10-11 0.12555411431165453 2117 12-13 0.1782072267108461 2117 14-15 0.2236015145031871 2117 16-17 0.18876954468436935 2117 18-19 0.158755048324565 2117 20-21 -0.04447338805367451 2117 22-23 0.24072923008289138 2117 24-25 -0.08379316480687748 2117 26-27 0.09419921670662035 2117 28-29 -0.3896820491070869 2117 30-31 0.1946425694345848 2117 32-33 0.09228975060258193 2117 34-35 0.22169699999579962 2117 36-37 0.22173658975363253 2117 38-39 0.02262658535124018 2117 40-41 0.1369007779893323 2117 42-43 0.16768705657143101 2117 44-45 0.24591919600353407 2117 46-47 0.3844624587755803 2117 48-49 0.10214417773209306 2117 50-51 0.0057724876183939955 2117 52-53 -0.03069619754322872 2117 54-55 0.28039382558420556 2117 56-57 0.13998653585077392 2117 58-59 0.3571135327956583 2117 60-61 0.033168156319931086 2117 62-63 0.3137167172318911 2117 64-65 0.23615350463540352 2117 66-67 -0.07945261397348702 2117 68-69 -0.11001576421277548 2117 70-71 0.06410586848754463 2117 72-73 -0.2580253105337391 2117 74-75 0.03449646551721486 2117 76 -0.485637505278504 2118 1 -0.08609544621437948 2118 2 -0.17450098418968452 2118 3 -0.2952192008892993 2118 4 -0.09213646397378739 2118 5 -0.12588586969469873 2118 6 -0.05995473987010058 2118 7 -0.04935567579687472 2118 8 -0.007185504941219278 2118 9 -0.12704806832581994 2118 10-11 -0.09160479894308793 2118 12-13 -0.04591919404378331 2118 14-15 0.01258617669104467 2118 16-17 -0.28054215515084024 2118 18-19 -0.307970772438523 2118 20-21 -0.09983739701129934 2118 22-23 -0.21835303636405712 2118 24-25 -0.17159051977371576 2118 26-27 0.02584243299934741 2118 28-29 -0.06430071644980018 2118 30-31 -0.15469194698067668 2118 32-33 -0.22318327090416545 2118 34-35 -0.18865919217051186 2118 36-37 -0.2774225770186689 2118 38-39 -0.10142378013657094 2118 40-41 -0.4278983093474693 2118 42-43 -0.03822835307068573 2118 44-45 -0.4108433848051902 2118 46-47 -0.15527641814163928 2118 48-49 -0.04691991995667166 2118 50-51 -0.04120431552522774 2118 52-53 0.08516132559024925 2118 54-55 0.14703514049526234 2118 56-57 0.12423887032493752 2118 58-59 -0.350453966253653 2118 60-61 -0.1776316915695375 2118 62-63 0.012668844666720247 2118 64-65 -0.20428254556107817 2118 66-67 0.24305551971477257 2118 68-69 0.23204702363528185 2118 70-71 0.45237645622574973 2118 72-73 0.3082188268727748 2118 74-75 0.10726867999306222 2118 76 -0.5030879637227486 2119 1 0.11335070392708957 2119 2 0.05351487449259906 2119 3 0.11431148875697517 2119 4 0.06649335963707159 2119 5 -0.07732940538963362 2119 6 0.02217672206992205 2119 7 0.00945217098279727 2119 8 0.00973959632558774 2119 9 0.08326007515913858 2119 10-11 -0.035084592883421806 2119 12-13 0.0229680321488388 2119 14-15 -0.4906603810976833 2119 16-17 -0.06173706042015681 2119 18-19 0.03851649503483401 2119 20-21 0.11785827797172033 2119 22-23 0.118618161723262 2119 24-25 0.07301073412860148 2119 26-27 0.15957051335917782 2119 28-29 0.23662293999128536 2119 30-31 0.10329974527144259 2119 32-33 -0.23402806394148 2119 34-35 0.17816743734349672 2119 36-37 0.09302041686662221 2119 38-39 -0.007308004875774543 2119 40-41 0.08475251583722354 2119 42-43 -0.06354290366969195 2119 44-45 -0.026377841318513617 2119 46-47 0.16649400937196646 2119 48-49 0.2942180522992359 2119 50-51 -0.04390046351666399 2119 52-53 0.14398637267608194 2119 54-55 0.12043486701039541 2119 56-57 0.0848114685171808 2119 58-59 0.1370930120825662 2119 60-61 0.20746596316871546 2119 62-63 0.14770414829511935 2119 64-65 0.20308950566143125 2119 66-67 0.10892753223659213 2119 68-69 -0.05401986835538963 2119 70-71 0.18513959940970182 2119 72-73 0.339807189145624 2119 74-75 0.576923039840672 2119 76 -0.6772496638010139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 2.0 20 2.0 21 5.0 22 11.0 23 13.0 24 30.0 25 56.0 26 125.0 27 235.0 28 442.0 29 719.0 30 1161.0 31 1730.0 32 2877.0 33 4781.0 34 7887.0 35 13320.0 36 19808.0 37 82476.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.82064548462939 15.81503674059006 12.600880005306564 36.76343776947399 2 18.377665258952984 17.719503836204037 37.76505184955889 26.13777905528408 3 17.941347720019753 17.222750421945594 33.34144058490135 31.494461273133307 4 14.80310434032768 24.127180666416077 31.47972081573691 29.58999417751933 5 15.702272241507654 29.16473198163339 39.317222013399075 15.81577376345988 6 28.966472829651902 37.76357780381925 15.924116125323367 17.345833241205476 7 26.921234365902375 23.8692226619792 21.917586102696767 27.291956869421657 8 23.30097802934825 37.123841952815795 15.0175779954452 24.557602022390753 9 21.67584260139592 14.873858535830367 16.3678038929548 47.08249496981891 10-11 26.182368938908173 26.33603820726557 22.89930056529654 24.582292288529715 12-13 24.886774444374836 18.013642343593958 25.782997556760183 31.316585655271023 14-15 21.89215881368799 17.136887257611605 19.852816532897016 41.11813739580339 16-17 28.48335433848512 24.177666732998727 17.90597062226841 29.433008306247743 18-19 31.218814719820752 22.718729962190725 18.082487599590216 27.979967718398303 20-21 25.424340917298665 19.995798969642028 18.32754770380525 36.25231240925406 22-23 24.490532941237166 24.217097456534077 18.016892564176263 33.27547703805249 24-25 29.744400468746544 24.808926820999254 19.03656370457175 26.410109005682447 26-27 29.86011305930823 27.31333053264643 19.751844399731723 23.074712008313618 28-29 30.018941487754365 23.018329758772417 19.01334748417243 27.949381269300787 30-31 30.267686706318496 19.50494173834214 24.458103934965102 25.769267620374258 32-33 25.555899499561473 24.472844392361495 25.92330540016657 24.047950707910466 34-35 26.874433413668825 26.00732600732601 23.447645580442362 23.670594998562805 36-37 26.658485712811668 24.477635041015322 20.05070717344359 28.813172072729415 38-39 21.751755956987346 22.889719267988887 26.354095267576156 29.004429507447615 40-41 22.429079974351605 18.07216927941274 28.19333583921109 31.305414907024563 42-43 27.826298450040905 17.974145237726727 26.99088302710033 27.20867328513204 44-45 21.99165690111364 24.028419601860247 29.82731554160126 24.152607955424855 46-47 25.87429337932356 20.46233444623787 26.775303837678084 26.88806833676049 48-49 25.865449104885723 18.8095606606673 22.699935879010326 32.62505435543665 50-51 23.53314023334144 19.460720366152962 26.931921197514757 30.07421820299084 52-53 19.137904349171954 19.19023297292915 35.457064732718656 26.21479794518024 54-55 19.710939630456732 20.37168063325005 30.715796611168845 29.201583125124376 56-57 24.173981618649627 21.461737457713312 24.48684782688807 29.87743309674899 58-59 20.887965153558717 21.587031345582652 30.479949292826557 27.045054208032077 60-61 24.721221099490716 25.997376198583446 25.023400476116777 24.258002225809065 62-63 20.154258886653253 34.83096380480686 20.566254670882437 24.448522637657447 64-65 19.026613895829186 37.25429500077387 19.37743678186334 24.3416543215336 66-67 19.113214083032997 36.77265055534673 19.31736941797304 24.79676594364723 68-69 19.077468473846743 37.21633832297816 19.52484135582727 24.181351847347823 70-71 19.497940021078854 35.73160575172648 19.80196195487946 24.96849227231521 72-73 19.78058829165469 32.489073635954924 20.81057775222765 26.919760320162734 74-75 19.97368828354744 31.14879754718789 22.16522578695617 26.7122883823085 76 20.232751822289046 31.31094257854821 21.925693354264784 26.53061224489796 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 2.5 20 5.5 21 6.0 22 15.0 23 27.0 24 39.5 25 49.0 26 106.0 27 199.5 28 277.0 29 318.0 30 419.0 31 605.5 32 849.5 33 1008.0 34 1188.5 35 1568.5 36 1865.0 37 1962.0 38 2281.5 39 3201.5 40 6666.5 41 10903.5 42 12276.0 43 11394.5 44 9339.5 45 7376.0 46 6586.0 47 6223.5 48 5463.5 49 4645.5 50 4225.0 51 4088.0 52 3416.0 53 2635.0 54 2389.0 55 2381.0 56 2346.0 57 2331.5 58 2344.0 59 2424.5 60 2762.0 61 3224.0 62 3429.0 63 3620.0 64 3897.0 65 4004.0 66 4004.5 67 3984.0 68 3968.5 69 3783.0 70 3430.0 71 3247.0 72 2921.5 73 2270.0 74 1624.0 75 1304.0 76 1040.5 77 554.0 78 217.0 79 103.0 80 60.5 81 10.0 82 2.0 83 2.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.6851143490982524E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 135681.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.709178145797864 #Duplication Level Percentage of deduplicated Percentage of total 1 56.21180099388816 21.759126185685542 2 22.24824355971897 17.224224467685232 3 9.236305477808877 10.725893824485373 4 4.124064659850346 6.385566144117452 5 2.122960339673654 4.108902499244552 6 1.3956322233011558 3.241426581466823 7 1.0186401629824262 2.760150647474591 8 0.7768321242931399 2.4056426470913395 9 0.5712000913920147 1.9899617485130563 >10 2.2524323603891774 12.732070076134464 >50 0.019040003046400487 0.5431858550570825 >100 0.013328002132480341 0.9898217141677906 >500 0.0019040003046400487 0.4849610483413301 >1k 0.005712000913920146 6.735652007281787 >5k 0.0 0.0 >10k+ 0.0019040003046400487 7.913414553253588 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 10737 7.913414553253588 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 3544 2.6120090506408413 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 2867 2.113044567772938 No Hit CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG 2728 2.0105983888680066 RNA PCR Primer, Index 34 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 658 0.4849610483413301 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT 365 0.26901334748417244 RNA PCR Primer, Index 24 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT 307 0.22626602103463267 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 148 0.10907938473330826 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 147 0.10834236186348861 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT 138 0.10170915603511177 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010318320177475106 0.0 2 0.0 0.0 0.0 0.01326641165675371 0.0 3 0.0 0.0 0.0 0.01400343452657336 0.0 4 0.0 0.0 0.0 0.030217937662605672 0.0 5 0.0 0.0 0.0 0.03537709775134323 0.0 6 0.0 0.0 0.0 0.03685114349098253 0.0 7 0.0 0.0 0.0 0.03685114349098253 0.0 8 0.0 0.0 0.0 0.03758816636080218 0.0 9 0.0 0.0 0.0 0.04495839505899868 0.0 10 0.0 0.0 0.0 0.050854578017555885 0.0 11 0.0 0.0 0.0 0.051591600887375536 0.0 12 0.0 0.0 0.0 0.052328623757195186 0.0 13 0.0 0.0 0.0 0.05527671523647379 0.0 14 0.0 0.0 0.0 0.05896182958557204 0.0 15 0.0 0.0 0.0 0.05969885245539169 0.0 16 0.0 0.0 0.0 0.060435875325211344 0.0 17 0.0 0.0 0.0 0.06485801254412925 0.0 18 0.0 0.0 0.0 0.0670690811535882 0.0 19 0.0 0.0 0.0 0.0714912183725061 0.0 20 0.0 0.0 0.0 0.07591335559142401 0.0 21 0.0 0.0 0.0 0.08107251568016155 0.0 22 0.0 0.0 0.0 0.08696869863871876 0.0 23 0.0 7.370228698196505E-4 0.0 0.09433892733691526 0.0 24 0.0 7.370228698196505E-4 0.0 0.10244617890493142 0.0 25 0.0 7.370228698196505E-4 0.0 0.10834236186348863 0.0 26 0.0 7.370228698196505E-4 0.0 0.12381984212970129 0.0 27 0.0 7.370228698196505E-4 0.0 0.21521067798733795 0.0 28 0.0 7.370228698196505E-4 0.0 0.3169198340224497 0.0 29 0.0 7.370228698196505E-4 0.0 0.43410647032377414 0.0 30 0.0 7.370228698196505E-4 0.0 0.560874403932754 0.0 31 0.0 7.370228698196505E-4 0.0 0.7031198178079466 0.0 32 0.0 7.370228698196505E-4 0.0 0.8888495810024986 0.0 33 0.0 7.370228698196505E-4 0.0 1.0981640760312792 0.0 34 0.0 7.370228698196505E-4 0.0 1.3929732239591395 0.0 35 0.0 7.370228698196505E-4 0.0 1.8101281682770616 0.0 36 0.0 7.370228698196505E-4 0.0 2.395324326913864 0.0 37 0.0 7.370228698196505E-4 0.0 3.0984441447218107 0.0 38 0.0 7.370228698196505E-4 0.0 3.8479964033283953 0.0 39 0.0 7.370228698196505E-4 0.0 4.614500187940831 0.0 40 0.0 7.370228698196505E-4 0.0 5.401640612908219 0.0 41 0.0 7.370228698196505E-4 0.0 6.124660048201296 0.0 42 0.0 7.370228698196505E-4 0.0 6.881582535506077 0.0 43 0.0 7.370228698196505E-4 0.0 7.586176399053663 0.0 44 0.0 7.370228698196505E-4 0.0 8.314354994435478 0.0 45 0.0 7.370228698196505E-4 0.0 9.136135494284387 0.0 46 0.0 7.370228698196505E-4 0.0 9.935805308038708 0.0 47 0.0 7.370228698196505E-4 0.0 10.694938863952949 0.0 48 0.0 7.370228698196505E-4 0.0 11.417221276376205 0.0 49 0.0 7.370228698196505E-4 0.0 12.075382699125154 0.0 50 0.0 7.370228698196505E-4 0.0 12.65247160619394 0.0 51 0.0 7.370228698196505E-4 0.0 13.250197153617677 0.0 52 0.0 7.370228698196505E-4 0.0 13.884036821662576 0.0 53 0.0 7.370228698196505E-4 0.0 14.53040587849441 0.0 54 0.0 7.370228698196505E-4 0.0 15.14287188331454 0.0 55 0.0 7.370228698196505E-4 0.0 15.743545522217554 0.0 56 0.0 7.370228698196505E-4 0.0 16.358959618516963 0.0 57 0.0 7.370228698196505E-4 0.0 16.959633257419977 0.0 58 0.0 7.370228698196505E-4 0.0 17.552199644754978 0.0 59 0.0 7.370228698196505E-4 0.0 18.121918323125566 0.0 60 0.0 7.370228698196505E-4 0.0 18.699007230194354 0.0 61 0.0 7.370228698196505E-4 0.0 19.31884346371268 0.0 62 0.0 7.370228698196505E-4 0.0 19.873821684686877 0.0 63 0.0 7.370228698196505E-4 0.0 20.43469608861963 0.0 64 0.0 7.370228698196505E-4 0.0 21.005888812729857 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCGT 20 7.903476E-5 69.99999 37 TCTATCG 20 7.903476E-5 69.99999 29 CTTTATA 85 0.0 61.764706 2 ATCGTAT 25 2.3835005E-4 56.000004 39 TATCGCC 25 2.3835005E-4 56.000004 31 GCATATC 25 2.3835005E-4 56.000004 35 TTTATAC 100 0.0 52.500004 3 CAAACGC 20 0.006938894 52.499996 19 CCCACGT 20 0.006938894 52.499996 59 TTCTATC 30 5.860984E-4 46.666664 28 GTTTGGC 30 5.860984E-4 46.666664 59 CATATCG 30 5.860984E-4 46.666664 36 CTATATC 30 5.860984E-4 46.666664 2 TATTGCG 30 5.860984E-4 46.666664 9 AAATTCA 30 5.860984E-4 46.666664 70 TAAATGG 30 5.860984E-4 46.666664 53 ACTTTAT 30 5.860984E-4 46.666664 41 TATCGTA 30 5.860984E-4 46.666664 38 TCTTTAT 115 0.0 45.652172 1 GTTGTCT 35 0.0012518581 40.0 70 >>END_MODULE