FastQCFastQC Report
Wed 25 May 2016
SRR1780287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486758
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCA353.2401312E-431.3869672
AATTGCC300.005698182629.37900419
TATCTCA300.00574423829.3306622
ACAGTAG300.005750015429.32462511
TACAGTA300.005750015429.32462510
ATAGCAA454.1015024E-529.2945063
TAGACCG300.00577897229.2945025
TATCCAC300.00577897229.2945023
TATAGCA655.98784E-727.041082
TAATACA609.837951E-625.632694
AAAGCAT450.001381913924.49007221
ATGCAGT450.001402788324.42714331
CTTGTAC901.5292244E-824.4120883
GTTCTAC450.001407836624.4120881
TTAATAC450.001407836624.4120883
GTACAAG551.6060755E-423.9682311
ACGAGAG703.197243E-522.01157824
TATTTCG500.002584230621.99120930
AAACTCG602.8771258E-421.99120927
CGAAACT602.8771258E-421.99120925
Produced by FastQC (version 0.11.5)