FastQCFastQC Report
Wed 25 May 2016
SRR1780287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486758
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT192913.9631603384022447No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC64891.333105978740976No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG50931.0463104869360134No Hit
CACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTTCTG37980.7802645256986018TruSeq Adapter, Index 2 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC11510.2364624721113983No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT5220.10724014808179835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTTA207.850287E-444.0019536
TTAATAC357.2793428E-637.7159582
TCTTCTG4700.035.57604644
TAGAACG250.002350904535.2015654
GTTTCGG250.002352089935.1979521
TCTTTAT1300.033.8998951
GTCTTCT5000.033.00146543
ATGCCGT26450.032.27364344
CCGTCTT5200.031.73217641
CTTTATA1550.031.2271942
CGTCTTC5600.029.46559142
AACGATG407.0284534E-427.4983967
TATGCCG31400.027.25598743
TAATACG450.001396654424.445534
CATGCTT450.001396654424.4455344
GATAATC450.001397490724.44301834
TGGTATC450.001397490724.44301822
GGTTTTC551.5923423E-423.99859838
GCCGTCT7000.023.57005340
TTTATAC2450.022.4499763
Produced by FastQC (version 0.11.5)